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Identification of biomarkers of human pancreatic adenocarcinomas by expression profiling and validation with gene expression analysis in endoscopic ultrasound-guided fine needle aspiration samples

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World Journal of Gastroenterology 2006年第21期12卷 3344-3351页

作者： Henrik Laurell Michèle Bouisson Philippe Berthelémy Philippe Rochaix Sébastien Déjean Philippe Besse Christiana Susini Lucien Pradayrol Nicole Vaysse Louis Buscail INSERM U531 IFR31 Institut Louis BugnardBP 8422531432 Toulouse Cedex 4France Service de Gastroentérologie Centre Hospitaller de Pau PauFrance Laboratoire d'Anatomo-Pathologie Institut Claudius Regaud ToulouseFrance Laboratoire de Statistique et Probabilités Université Paul Sabatier ToulouseFrance INSERM U531 IFR31 Institut Louis BugnardBP 8422531432 Toulouse Cedex 4France Service de Gastroenterologie et de la NutritionCHU RangueilToulouseFrance

AIM： To compare gene expression profiles of pancreatic adenocarcinoma tissue specimens, human pancreatic and colon adenocarcinoma and leukemia cell lines and normal pancreas samples in order to distinguish differentially expressed genes and to validate the differential expression of a subset of genes by quantitative real-time RT-PCR （RT-QPCR） in endoscopic ultrasound-guided fine needle aspiration （EUS-guided FNA） ***： Commercially dedicated cancer cDNA macroarrays （Atlas Human Cancer 1.2） containing 1176 genes were used. Different statistical approaches （hierarchical clustering, principal component analysis （PCA） and SAM） were used to analyze the expression data. RT-QPCR and immunohistochemical studies were used for validation of ***： RT-QPCR validated the increased expression of LCN2 （lipocalin 2） and for the first time PLAT （tissue-type plasminogen activator or tPA） in malignant pancreas as compared with normal pancreas. Immunohistochemical analysis confirmed the increased expression of LCN2 protein localized in epithelial cells of ducts invaded by carcinoma. The analysis of PLAT and LCN2 transcripts in 12 samples obtained through EUS-guided FNA from patients with pancreatic adenocarcinoma showed significantly increased expression levels in comparison with those found in normal tissues, indicating that a sufficient amount of high quality RNA can be obtained with this ***： Expression profiling is a useful method to identify biomarkers and potential target genes. Molecular analysis of EUS-guided FNA samples in pancreatic cancer appears as a valuable strategy for the diagnosis of pancreatic adenocarcinomas.

关键词： Pancreas Colon Adenocarcinoma Gene expression profiling Endoscopic ultrasonography Ultrasound fine needle aspiration

Large leiomyoma of lower esophagus diagnosed by endoscopic ultrasonography–fine needle aspiration:A case report

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World Journal of Clinical Cases 2020年第22期8卷 5809-5815页

作者： Min Rao Qing-Qing Meng Pu-Jun Gao Department of Hepatology and Gastroenterology The Second Part of First Hospital of Jilin UniversityChangchun 130021Jilin ProvinceChina Department of Hepatology The First Hospital of Jilin UniversityChangchun 130021Jilin ProvinceChina

BACKGROUND Benign esophageal tumors are rare accounting for5 cm are *** describe a case of a large esophageal leiomyoma involving the cardia and *** SUMMARY A 35-year-old woman presented to the doctor because of a choking sensation after *** examination showed no positive *** indicated an uplifted change in the *** computed tomography revealed spaceoccupying lesions in the lower part of the esophagus and cardia,which were likely to be *** emission tomography–computed tomography showed increased metabolism of soft tissue masses in the lower esophagus and near the *** lesions were considered,and mesenchymal tumors were not *** ultrasonography was performed to examine a hypoechoic mass in the lower esophagus,which was unclear from the esophageal *** evaluation suggested diagnosis of esophageal and cardiac stromal ***,histological specimens obtained by endoscopic ultrasonography–fine needle aspiration suggested *** patient underwent laparoscopic local resection of the *** postoperative pathological diagnosis was *** Endoscopic ultrasonography-fine needle aspiration is necessary for the diagnosis of gastrointestinal *** provides a strong basis for diagnosis of gastrointestinal tumors of unknown nature and origin.

关键词： Esophageal tumor Leiomyoma Endoscopic ultrasonography fine needle aspiration Endoscopic ultrasonography-fine needle aspiration Case report

Endoscopic ultrasound fine needle aspiration vs fine needle biopsy in solid lesions:A multi-center analysis

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World Journal of Clinical Cases 2021年第34期9卷 10507-10517页

作者： Diogo Turiani Hourneaux Moura Thomas R McCarty Pichamol Jirapinyo Igor Braga Ribeiro Galileu Ferreira Ayala Farias Antonio Coutinho Madruga-Neto Marvin Ryou Christopher C Thompson Gastrointestinal Endoscopy Unit University of Sao Paulo School of MedicineSão PauloSP 05403-010Brazil Division of Gastroenterology Hepatology and EndoscopyBrigham and Women’s HospitalHarvard Medical SchoolBostonMA 02115United States Division of Gastrointestinal Endoscopy University of São Paulo Medical SchoolSão PauloSP 01246-903Brazil

BACKGROUND While endoscopic ultrasound(EUS)-guided fine needle aspiration(FNA)is considered a preferred technique for tissue sampling for solid lesions,fine needle biopsy(FNB)has recently been *** To compare the accuracy of FNB vs FNA in determining the diagnosis of solid *** A retrospective,multi-center study of EUS-guided tissue sampling using FNA vs FNB *** outcomes included diagnostic test characteristics(i.e.,sensitivity,specificity,accuracy),use of rapid on-site evaluation(ROSE),and adverse *** analyses were performed by type of lesion and diagnostic yield with or without ROSE.A multivariable logistic regression was also *** A total of 1168 patients with solid lesions(n=468 FNA;n=700 FNB)underwent EUS-guided *** age was 65.02±12.13 ***,sensitivity,specificity and accuracy were superior for FNB vs FNA(84.70%vs 74.53%;99.29%vs 96.62%;and 87.62%vs 81.55%,respectively;P0.05).Multivariate analysis showed no significant predictor for better *** subgroup analyses,FNB was superior to FNA for non-pancreatic lesions;however,there was no difference between the techniques among pancreatic *** adverse event was reported in each *** FNB is superior to FNA with equivalent diagnostic test characteristics compared to FNA+ROSE in the diagnosis of non-pancreatic solid *** results suggest that EUS-FNB may eliminate the need of ROSE and should be employed as a first-line method in the diagnosis of solid lesions.

关键词： Endoscopic ultrasound-guided tissue acquisition fine needle aspiration fine needle biopsy Solid lesions Endoscopic ultrasound Cancer

Resected tumor seeding in stomach wall due to endoscopic ultrasonography-guided fine needle aspiration of pancreatic adenocarcinoma

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World Journal of Gastroenterology 2015年第27期21卷 8458-8461页

作者： Akiko Tomonari Akio Katanuma Tomoaki Matsumori Hajime Yamazaki Itsuki Sano Ryuki Minami Manabu Sen-yo Satoshi Ikarashi Toshifumi Kin Kei Yane Kuniyuki Takahashi Toshiya Shinohara Hiroyuki Maguchi Center for Gastroenterology Teine-Keijinkai Hospital Department of Gastroenterology and Hepatology Kyoto University Graduate School of Medicine Department of Pathology Teine-Keijinkai Hospital

Endoscopic ultrasonography-guided fine needle aspiration(EUS-FNA) is a useful and relatively safe tool for the diagnosis and staging of pancreatic cancer. However, there have recently been several reports of tumor seeding after EUS-FNA of adenocarcinomas. A 78-year-old man was admitted to our hospital due to upper gastric pain. Examinations revealed a 20 mm mass in the pancreatic body, for which EUS-FNA was performed. The cytology of the lesion was adenocarcinoma, and the stage of the cancer was T3N0M0. The patient underwent surgery with curative intent, followed by adjuvant chemotherapy with S-1. An enlarging gastric submucosal tumor was found on gastroscopy at 28 mo after surgery accompanied by a rising level of CA19-9. Biopsy result was adenocarcinoma, consistent with a pancreatic primary tumor. Tumor seeding after EUS-FNA was strongly suspected. The patient underwent surgical resection of the gastric tumor with curative intent. The pathological result of the resected gastric specimen was adenocarcinoma with a perfectly matched mucin special stain result with the previously resected pancreatic cancer. This is the first case report of tumor seeding after EUS-FNA which was surgically resected and inspected pathologically.

关键词： Endoscopic ultrasonography-guided fine needle aspiration Tumor seeding Pancreatic cancer

Ultrasound-guided fine needle aspiration of retropharyngeal lymph nodes after radiotherapy for nasopharyngeal carcinoma: a novel technique for accurate diagnosis

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Cancer Communications 2018年第1期38卷 229-236页

作者： Long-Jun He Chuanbo Xie Yin Li Lin-Na Luo Ke Pan Xiao-Yan Gao Li-Zhi Liu Jian-Ming Gao Guang-Yu Luo Hong-Bo Shan Ming-Yuan Chen Chong Zhao Wei-Jun Fan Ping Yang Guo-Liang Xu Jian-Jun Li State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer MedicineGuangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and TherapySun Yat-sen University Cancer CenterGuangzhou 510060GuangdongP.R.China Department of Endoscopy Sun Yat-sen University Cancer CenterGuangzhou 510060GuangdongP.R.China Department of Cancer Prevention Research Sun Yat-sen University Cancer CenterGuangzhou 510060GuangdongP.R.China Department of Melanoma Medical Oncology The University of Texas MD Anderson Cancer CenterHoustonTX 77054USA Department of Imaging and Invention Radiology Sun Yat-sen University Cancer CenterGuangzhou 510060Guang-dongP.R.China Department of Radiation Oncology Sun Yat-sen University Cancer CenterGuangzhou 510060GuangdongP.R.China Department of Nasopharyngeal Oncology Sun Yat-sen University Cancer CenterGuangzhou 510060GuangdongP.R.China Department of Pathology Sun Yat-sen University Cancer CenterGuangzhou 510060GuangdongP.R.China

Background:Enlarged retropharyngeal lymph nodes(RLNs)are very common in patients with nasopharyngeal carcinoma(NPC)undergoing *** most suitable treatment option for enlarged RLNs depends on the pathological ***,RLN sampling is difficult and imminent in the clinic *** recently developed a novel minimally invasive technique termed endoscopic ultrasound-guided fine needle aspiration(EUS-FNA)for sam-pling RLN tissues sufficient for pathological or cytological ***:We enrolled 30 post-radiotherapy patients with NPC with suspected RLN metastasis detected via mag-netic resonance imaging(MRI).The EUS probe was introduced into the nasopharynx via the nostrils,and EUS was then used to scan the retropharyngeal space and locate the RLN in the anterior carotid ***-FNA was subsequently *** safety and efficacy of using EUS-FNA to sample the RLN tissues were ***:Strips of tissue were successfully sampled from all patients using *** the 30 patients,23 were confirmed to have cancer cells in the biopsied tissues via pathology or cytology examinations with 1 EUS-FNA biopsy *** seven cases without confirmed cancer cells were subsequently reanalyzed by using another EUS-FNA biopsy session,and two more cases were confirmed possessing cancer *** other five patients without con-firmed cancer cells were closely followed with MRI every month for 3 *** follow-up for 3 months,three patients were still considered cancer-free due to the presence of RLNs with stable or shrinking *** rest two patients who showed progressive disease underwent a third EUS-FNA biopsy procedure and were further confirmed to be cancer *** the whole cohort reported here,the EUS-FNA procedure was not associated with any severe ***:EUS-FNA is a safe and effective diagnostic approach for sampling tissues from the RLNs in patients with suspected recurrent NPC.

关键词： fine needle aspiration Endoscopic ultrasonography Retropharyngeal lymph node Nasopharyngeal carcinoma

Randomized controlled study of the safety and efficacy of nitrous oxide-sedated endoscopic ultrasound-guided fine needle aspiration for digestive tract diseases

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World Journal of Gastroenterology 2016年第46期22卷 10242-10248页

作者： Cai-Xia Wang Jian Wang Yuan-Yuan Chen Jia-Ni Wang Xin Yu Feng Yang Si-Yu Sun Endoscopy Center Shengjing Hospital of China Medical University Endoscopic Center Liao Dongwan Branch Courts Shengjing Hospital of China Medical University

AIM To evaluate the efficacy and safety of nitrous oxidesedated endoscopic ultrasound-guided fine needle aspiration. METHODS Enrolled patients were divided randomly into an experimental group(inhalation of nitrous oxide) and a control group(inhalation of pure oxygen) and heart rate, blood oxygen saturation, blood pressure, electrocardiogram(ECG) changes, and the occurrence of complications were monitored and recorded. All patients and physicians completed satisfaction questionnaires about the examination and scored the process using a visual analog scale. RESULTS There was no significant difference in heart rate, blood oxygen saturation, blood pressure, ECG changes, or complication rate between the two groups of patients(P > 0.05). However, patient and physician satisfaction were both significantly higher in the nitrous oxide compared with the control group(P fine needle aspiration.

关键词： Endoscopic ultrasonography Nitrous oxide Sedation fine needle aspiration

The efficacy of rapid on-site evaluation during endoscopic ultrasound-guided fine needle aspiration of pancreatic masses

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Gastroenterology Report 2018年第1期6卷 45-48,I0002页

作者： Abhinav Koul Anand C.Baxi Ruilian Shang Xianmei Meng Lianyong Li Steven A.Keilin Field F.Willingham Qiang Cai Department of Medicine Medical College of GeorgiaAthensGAUSA Department of Medicine University of Washington School of MedicineSeattleWAUSA Department of Digestive Diseases General Hospital of Jinan Military RegionJinanShandongChina Department of Gastroenterology Baotou Medical College Second Affiliated HospitalBaotouNeimengChina Department of Gastroenterology PLA Hospital 306BeijingChina Division of Digestive Diseases Emory University School of MedicineAtlantaGAUSA

Background:Endoscopic ultrasound(EUS)-guided fine needle aspiration(FNA)has become the preferred method to diagnose pancreatic masses due to its minimally invasive approach and diagnostic *** studies have shown that rapid on-site evaluation(ROSE)improves diagnostic yield by 10–30%;however,more recent studies have demonstrated effective diagnostic accuracy rates without *** study aims to examine whether the current standard of performing ROSE after each FNA pass adds diagnostic value during EUS-guided FNA of pancreatic ***:We conducted a retrospective case series on patients who underwent EUS-guided FNA of pancreatic masses between February 2011 and October *** cases were performed by one of three endoscopists at Emory University *** demographics,radiologic details of pancreatic masses and pathology reports of the biopsied pancreatic masses were ***:A total of 184 procedures performed in 171 patients were *** final pathology reports of the biopsied pancreatic masses showed 128(70%)with confirmed *** 64(50%)of these 128 cases initially showed malignant cells during *** these 64 cases,23%required 5 or more FNA passes to first detect malignant ***:The use of ROSE during EUS-guided FNA of pancreatic masses may increase the diagnostic yield,since malignant cells were often detected during later FNA passes that would otherwise be missed if tissue sampling stopped *** addition,sample preparation for ROSE may be suboptimal,since malignant cells were only detected in 50%of cases.

关键词： endoscopic ultrasound fine needle aspiration rapid on-site evaluation pancreatic mass

The Value of Clinical Breast Examination, Imaging, and fine needle aspiration and the Challenge of Diagnosing Breast Cancer in a Low Resource Setting: A Hospital-Based Analytical Study in Yaounde

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Advances in Breast Cancer Research 2022年第3期11卷 173-182页

作者： C. E. Ebong N. J. D. Kemfang E. Atenguena F. Essiben J. H. Fouedjio A. Ngassam S. Nyada N. F. Mangala A. N. Ngalame J. T. Fouogue S. Dohbit Faculty of Medicine and Biomedical Sciences University of Yaoundé 1 Yaoundé Cameroon Central Hospital of Yaoundé Yaoundé Cameroon General Hospital of Yaoundé Yaoundé Cameroon Gynecological Endoscopic Surgery and Human Reproduction Teaching Hospital of Yaoundé Yaoundé Cameroon Faculty of Medicine and Pharmaceutical Sciences University of Douala Douala Cameroon Gyneco-Obstetric and Pediatric Hospital of Douala Douala Cameroon Faculty of Medicine and Pharmaceutical Sciences of the University of Dschang Dschang Cameroon Faculty of Health Sciences University of Buea Buea Cameroon

Background: Breast cancer is the most common cancer in the world, as well as in Cameroon, where it represents about 20.1% of all cancers recorded in 2020. The number of pathologists in the country is as few as seven for a population of about 26 million. The diagnostic performances of diagnostic modalities other than histology—clinical breast examination (CBE), imaging and fine needle aspiration and cytology (FNA)—in our context are not known. Study Objectives: Our objectives were to estimate the proportions of cases managed with mammography, breast ultrasound and FNA and to estimate the sensitivity, specificity, positive and negative predictive values, and accuracy of CBE, mammography, breast ultrasound and FNA;using histology as reference. Study Methodology: The study was cross-sectional and analytical, and was carried out at the Yaoundé General Hospital. It lasted twelve months, April 2015 through March 2016 and covered the period January 2010 to February 2016. Using histology as reference, we calculated measures of diagnostic accuracy for all four modalities using the statistical methods of Galen and Gambino. Results: We recruited 107 cases, 105 females (98.1%) and 02 males. We had 112 breast lumps, 106 malignant (94.6%) and 6 benign. The mean lump size was 61.1 mm. The most frequently used diagnostic tool after CBE was FNA (49.1%), while the diagnostic accuracies were 76.8%, 79.1%, 82.9%, and 82.0% for CBE, breast US, mammography and FNA. Conclusion: The four baseline diagnostic modalities for breast cancer are used sub-optimally and FNA appears to be the most commonly used in our setting after CBE. We recommend that FNA should be considered for diagnosis as appropriate but a negative result should not stop the quest for histological elimination of presence of malignancy.

关键词： Breast Cancer Lump Clinical Breast Examination Breast Ultrasound Mam-mography fine needle aspiration Accuracy Cameroon

Endoscopic ultrasound-guided fine-needle aspiration for suspected malignancies adjacent to the gastrointestinal tract

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World Journal of Gastroenterology 2014年第26期20卷 8599-8605页

作者： Pietro Gambitta Antonio Armellino Edoardo Forti Maurizio Vertemati Paola Enrica Colombo Paolo Aseni Unità di Chirurgia Endoscopica ed Ecoendoscopia Ospedale Niguarda Ca’ Granda Dipartimento di Scienze Biomediche e Cliniche "L. Sacco" Università degli Studi di Milano Servizio di Fisica Sanitaria Ospedale Niguarda Ca’ Granda HPB and Transplantation Surgery Ospedale Niguarda Ca’ Granda

AIM: To investigate the impact of endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) in association with a multidisciplinary team evaluation for the detection of gastrointestinal malignancies.

关键词： Gastrointestinal neoplasm Endoscopic ultrasonography Clinical scoring system fine needle aspiration Clinical decision support system