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An effective inducer of dopaminergic neuron-like differentiation

Neural Regeneration Research 2013年第5期8卷 427-434页

作者： Wenyu Fu Cui Lv Wenxin Zhuang Dandan Chen E Lv Fengjie Li Xiaocui Wang Department of Histology and Embryology Weifang Medical University Stem Cell Research and Transplantation Center Affiliated Hospital of Weifang Medical University

Rat bone marrow-derived mesenchymal stem cells were cultured and passaged in vitro. After induction with basic fibroblast growth factor for 24 hours, passage 3 bone marrow-derived mesenchymal stem cells were additionally induced into dopaminergic neurons using three different combinations with basic fibroblast growth factor as follows： 20% Xiangdan injection; ali-trans retinoic acid ＋ glial-derived neurotrophic factor; or sonic hedgehog ＋ fibroblast growth factor 8. Results suggest that the bone marrow-derived mesenchymal stem cells showed typical neuronal morphological characteristics after induction. In particular, after treatment with sonic hedgehog ＋ fibroblast growth factor 8, the expressions of nestin, neuron-specific enolase, microtubule- associated protein 2, tyrosine hydroxylase and vesicular monoamine transporter-2 in cells were significantly increased. Moreover, the levels of catecholamines in the culture supernatant were significantly increased. These findings indicate that Xiangdan injection, all-trans retinoic acid ＋ glial-derived neurotrophic factor, and sonic hedgehog ＋ fibroblast growth factor 8 can all induce dopaminergic neuronal differentiation from bone marrow-derived mesenchymal stem cells. In particular, the efficiency of sonic hedgehog ＋ fibroblast growth factor 8 was highest.

关键词： neural regeneration stem cells mesenchymal stem cells dopaminergic neuron induction differentiation Xiangdan injection all-trans retinoic acid sonic hedgehog fibroblast growth factor 8 catecholamine grants-supported paper photographs-containing paper neuroregeneration

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