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An exploratory study of South African women’s experiences of In Vitro fertilisation and Embryo Transfer (IVE-ET) at fertility clinics

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Open Journal of Preventive Medicine 2013年第8期3卷 470-478页

作者： Athena Pedro Kelvin Mwaba Department of Psychology University of the Western Cape Bellville South Africa

Infertility is considered to be a growing problem worldwide. In sub-Saharan Africa, at least 20%-50% of couples of reproductive age experience a fertility problem and 30% are diagnosed with infertility. This study explores the experiences of women in South Africa who are involuntary childless and explores their psychological and emotional experiences of In Vitro fertilisation and Embryo Transfer (IVF-ET). Utilising a qualitative methodology, a diverse group of 21 married women diagnosed with infertility and who had undergone at least two cycles of IVF-ET were recruited. Semi-structured, in-depth individual interviews were conducted and the data were analysed using thematic analysis. The results of the study indicated that the women perceived themselves as not conforming to a dominant belief system and as a result felt compelled to explore all the medical options available. They reported emotional turmoil characterised by primary binary emotions of anxiety-excitement and nervousness-optimistic. These emotions were experienced throughout the five stages of the IVF-ET treatment cycles. A synopsis of the psychological and emotional responses to the IVF-ET treatment is discussed. The findings of this study suggest the need for the incorporation of a mandatory psychosocial intervention as part of infertility management. Greater attention to the psychological and emotional repercussions of infertility treatment could lead to a more personalised client-approach which, in turn, would prepare infertile women and couples for the emotional demands of the treatment.

关键词： Infertility In Vitro fertilisation and Embryo Transfer Social Constructionist Psychological and Emotional

Acrosome reaction： relevance of zona pellucida glycoproteins

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Asian Journal of Andrology 2011年第1期13卷 97-105页

作者： Satish K Gupta Beena Bhandari Reproductive Cell Biology Laboratory National Institute of Immunology New Delhi-110 067 India

During mammalian fertilisation, the zona pellucida （ZP） matrix surrounding the oocyte is responsible for the binding of the spermatozoa to the oocyte and induction of the acrosome reaction （AR） in the ZP-bound spermatozoon. The AR is crucial for the penetration of the ZP matrix by spermatozoa. The ZP matrix in mice is composed of three glycoproteins designated ZP1, ZP2 and ZP3, whereas in humans, it is composed of four （ZP1, ZP2, ZP3 and ZP4）. ZP3 acts as the putative primary sperm receptor and is responsible for AR induction in mice, whereas in humans （in addition to ZP3）, ZP1 and ZP4 also induce the AR. The ability of ZP3 to induce the AR resides in its C-terminal fragment. O-linked glycans are critical for the murine ZP3-mediated AR. However, N-linked glycans of human ZP1, ZP3 and ZP4 have important roles in the induction of the AR. Studies with pharmacological inhibitors showed that the ZP3-induced AR involves the activation of the Gi-coupled receptor pathway, whereas ZP1- and ZP4-mediated ARs are independent of this pathway. The ZP3-induced AR involves the activation of T-type voltage-operated calcium channels （VOCCs）, whereas ZP1- and ZP4-induced ARs involve both T- and L-type VOCCs. To conclude, in mice, ZP3 is primarily responsible for the binding of capacitated spermatozoa to the ZP matrix and induction of the AR, whereas in humans （in addition to ZP3）, ZP1 and ZP4 also participate in these stages of fertilisation.

关键词： acrosome reaction fertilisation oocyte signalling pathways spermatozoa zona pellucida glycoproteins

Heat shock protein family D member 1 in boar spermatozoa is strongly related to the litter size of inseminated sows

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Journal of Animal Science and Biotechnology 2022年第4期13卷 1045-1055页

作者： Won-Ki Pang Ji-Hyun Son Do-Yeal Ryu Md Saidur Rahman Yoo-Jin Park Myung-Geol Pang Department of Animal Science&Technology and BET Research Institute Chung-Ang UniversityAnseongGyeonggi-do 17546Republic of Korea

Background:Sperm quality evaluation is the logical first step in increasing field *** contain cytoplasmic organelles and biomolecules known as sperm-intrinsic factors,which play key roles in sperm maturation,sperm-oocyte fusion,and embryo *** particular,sperm membrane proteins[e.g.,arginine vasopressin receptor 2,beta-actin,prohibitin,and heat shock protein family D member 1(HSPD1)]and RNA could be used as functional indicators of male *** sought to clarify the effects of differential mRNA expression of selected genes on several fertilisation parameters,including sperm motility,motion kinematics,capacitation,and litter size,in a porcine ***:Our results demonstrated that HSPD1 expression was significantly correlated with male fertility,as measured by the litter size of inseminated *** expression of HSPD1 mRNA was linked to sperm motility and other motion kinematic ***,HSPD1 had a 66.7%overall accuracy in detecting male fertility,and the high-litter size group which was selected with the HSPD1 marker had a 1.34 greater litter size than the lowlitter size ***:Our findings indicate that HSPD1 might be a helpful biomarker for superior boar selection for artificial insemination,which could boost field fertility.

关键词： fertilisation HSPD1 Male fertility Sperm motility Sperm RNA

Biomass Accumulation and Nutrient Uptake of Jerusalem Artichoke (<i>Helianthus tuberosus</i>L.)

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American Journal of Plant Sciences 2013年第8期4卷 1629-1640页

作者： Zoltán Izsáki Gabriella Németh Kádi Institute of Environmental Sciences Faculty of Economics Agricultural and Health Studies Szent István University Szarvas Hungary.

The dynamics of biomass accumulation during the growing period, the yield of leafy stalks and tubers, and the nutrient concentration and nutrient uptake of the yield were investigated for two Jerusalem artichoke varieties (Tápiói Korai and Tápiói Sima) in a field experiment involving mineral fertilisation. Considerable differences were observed between the dynamics of leafy stalk and tuber development in Tápiói Korai which has a short vegetation period and Tápiói Sima where the vegetation period is long. The maximum dry matter ratio between the tuber yield and the leafy stalk yield was 1:1 for Tápiói Korai and 1:4.5 for Tápiói Sima. During the period when the maximum aboveground biomass developed in Tápiói Korai, 100 ***-1 N and P fertiliser resulted in the highest leafy stalk yield (38.34 ***-1), while for Tápiói Sima, which developed a much greater leafy stalk mass, the highest aboveground biomass yield (78-80 ***-1) was given in response to 200 ***-1 N supplemented by P and K fertiliser. Both artichoke varieties produced the great-est tuber yield at a N rate of 200 ***-1, supplemented with P and K fertiliser. The nutrient concentration in the leafy stalks was highest on the 85th day of the vegetation period, prior to intensive dry matter accumulation in the leafy stalks and before tuber formation began. In both varieties the maximum nutrient uptake was recorded on the 155th day. Great differences were observed between the varieties in terms of specific nutrient uptake. For a tuber yield of 10 t, together with the corresponding leafy stalk yield, the specific nutrient uptake of the Tápiói Korai variety amounted to 48 kg N, 10 kg P, 83 kg K, 30 kg Ca and 10 kg Mg, while for Tápiói Sima these figures were 162 kg N, 30 kg P, 300 kg K, 84 kg Ca and 45 kg Mg.

关键词： Jerusalem Artichoke Mineral fertilisation Biomass Accumulation Yield Nutrient Uptake