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Asian Pacific Journal of Tropical Biomedicine 2012年第9期2卷 679-683页

作者： Ernieenor Faraliana Che Lah Raja Noor Azreen Raja Musa Ho Tze Ming Acarology Unit Infectious Disease Research CentreInstitute for Medical Research

Objective:To examined the immediate and 24 hours post-irradiation germicidal effects of UV-C lamp *** and adults of house dust mites Dermatophagoides pteronyssinus(***)and Dermatophagoides farinae(***).Methods:This study investigated the immediate and24 hours post irradiation mortalities of adult mites exposed to UV-C at different exposure times(5mins,10 mins,15 mins,20 mins,30 mins and 60 mins)and distances(10 cm,25 cm,33 cm,45 cm and 55 cm).Fresh eggs of the 2 dust mites were also irradiated at 10,35 and 55 cm for 0.5,1,2,3,and 5 minutes,and observed daily post-irradiation for up to 7 ***:Highest immediate mortality of 100%occurred with direct irradiation al 10 cm distance from UV-C lamp and for 60mins,for both species of *** post 24 hours mean mortality rates were(58.4±17.4)%for *** and(27.7±9.7)%for *** when irradiated for 1 hour at 55 cm distance under UV-C *** mites were irradiated in the presence of culture media,the highest mortality rates were lower compared to the direct irradiation;at 10 cm distance and 60 mins exposure,the mean mortality was(74.0±6.8)%for *** and(70.3±6.7)%for *** hatchability for both species of mites was also notably reduced by greater than 50%following ***:Ultraviolet C irradiation is lethal to an array of organisms by damaging their nucleic acids(DNA and RNA).This study demonstrates the increasing mite mortalities with increasing exposure times and decreasing distances.

关键词： Physical control UV-irradiation Dermatophagoides farinae Dermatophagoides pteronyssinus Mortality

Effect of a commercial air ionizer on dust mites Dermatophagoides pteronyssinus and Dermatophagoides farinae(Acari:Pyroglyphidae) in the laboratory

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Asian Pacific Journal of Tropical Biomedicine 2012年第2期2卷 156-158页

作者： Suhaili Zainal Abidin Ho Tze Ming Acarology Unit Infectious Diseases Research CentreInstitute for Medical Research

Objective:To investigate the short and long tenn efficacy of a commercial air ionizer in killing Dermatophagoides pteronyssinus(***)and Dermalophagoides farinae(***)***:The effect of a commercial ionizer on *** and *** was evaluated in the laboratory,using a specially designed *** was assessed after 6,16and 24 hours for direct exposure and after 24,36,48,60 and 72 hours for exposure in simulated *** batches of mites were used for each exposure ***:LT_(50)for direct exposure of ionizer was 10 hours for *** and 18 hours for *** LT_(50)for exposure in simulated mattress was 132 hours or 5.5 days for *** and 72 hours or 3days for ***_(95)for direct exposure of ionizer was 36 hours for *** and ***,the LT_(95)for exposure in simulated mattress was 956 hours or 39.8 days for *** and 403 hours or 16.8 days for ***:This study demonstrates the increasing mite mortalities with increasing exposure time of a commercial ionizer and suggests that negative ions produced by an ionizer kill dust mites and can be used to reduce natural mile populations on exposed surfaces such as floors,clothes,curtains,***,there is reduced efficacy on mites inside stuffed materials as in mattresses and furniture.

关键词： Dermatophagoides pteronyssinus Dermatophagoides farinae Direct exposure Simulated mattress Air ionizer Dust mite Negative ions Natural mite

Acaricidal activity of Cymbopogon citratus and Azadirachta indica against house dust mites

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Asian Pacific Journal of Tropical Biomedicine 2011年第5期1卷 365-369页

作者： Azima Laili Hanifah Siti Hazar Awang Ho Tze Ming Suhaili Zainal Abidin Maizatul Hashima Omar Acarology Unit Infectious Disease Research CenterInstitute for Medical ResearchJalan Pahang Phytochemistry Unit Herbal Medical Research CenterInstitute for Medical ResearchJalan Pahang

Objective:To examine the acaricidal effects of the essential oil of Cymbopogon citratits leaf extract(lemongrass)and ethanolic Azadirachta indica leaf extract(neem)against house dust mites Dermatophagoides farinae(***)and Dermalophagoides pteronyssinus(***).Methods:Twenty-five adults mites were placed onto treated filter paper that is soaked with plant extract and been tested at different concentrations(50.00%,25.00%,12.50%,6.25%and 3.13%)and exposure times(24hrs,48hrs,72hrs and 96 hrs).All treatments were replicated 7 times,and the experiment repeated *** topical and contact activities of the two herbs were ***:Mortalities from lemongrass extract were higher than neem for both topical and contact *** 50%concentration,both 24 hrs topical and contact exposures to lemongrass resulted in more than 91%mortalities for both species of *** the same concentration and exposure time,neem resulted in topical mortalities of 40.3%and 15.7%against *** and *** respectively;contact mortalities were 8.0%and 8.9%against the 2 mites,*** was no difference in topical mortalities of *** from exposure to concentrations oflemongrass and neem up to 12.50%;lemongrass was more effective than neem at the higher ***:Generally,topical mortalities of *** due to lemongrass are higher than that due to *** mortalities of lemongrass are always higher that neem against both species of mites.

关键词： Acaricidal Lemongrass Neem House dust mites Cymbopogon citratus Azadirachta indica Dermatophagoides farinae Dermatophagoides pteronyssinus

T cell responses to der f2 mite allergens in Thai allergic patients

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Health 2011年第7期3卷 423-431页

作者： Duangthep Thongdee Jundee Rabablert Tharit Muninnobpamas Piyalarp Wasuwat Rachanoo Pipatchaipaisan Supathra Tiewchareon Nat Malainual 不详

Dermatophagoides farinae group 2 (Der f2) is a highly polymorphic allergen that shows a distinct pattern of sequence divergence. The effect of the variants on antibody and T cell responses has not been compared. The aim of the present study was to evaluate IgE binding, transcription and translations for IL-5, IFN-γ and TGF-β induced by mite allergens. Sera from 24 HDM-allergic patients and 20 non-allergic subjects were measured for IgE reactivity by ELISA. PBMC was cultured with mite allergens (Df, rDerf2) and mitogen (PHA). The supernatants and cell pellet obtained were evaluated for cytokine production by ELISA and cytokine gene expression by RT-PCR, respectively. Four patients showed IgE reactivity to both allergens. Five patients showed IgE reactivity to Df. Other allergic patients and all non-allergic subjects did not show IgE reactivity to mite allergens. Both allergens showed similar levels of IL-5 and IFN-γ transcriptions in allergic patients and non-allergic subjects. The rDer f2 induced IL-5 protein from allergic patients higher than non-allergic subjects, while Df showed IL-5 protein from allergic patients similar to non-allergic subjects. Df induced IFN-protein from allergic patients higher than non-allergic subjects whereas rDer f2 induced IFN-protein from allergic patients similar to non-allergic subjects. The ratio of IFN-γ to IL-5 production after stimulation with rDer f2 was higher in non-allergic subjects than in allergic patients. Our data demonstrated that the changes in the sequence of rDer f2 compared with native Df had effect on cytokine production in both allergic patients and non-allergic subjects.

关键词： Dermatophagoides farinae IgE IL-5 IFN-γ TGF-β