检索结果-南通市图书馆

Chinese Journal of Chemistry 2000年第4期18卷 500-505页

作者：陆长元韩镇辉屠铁城苗金玲姚思德林念芸 Laboratory of Radiation Chemistry Shanghai Institute of Nuclear Research Chinese Academy of Sciences Shanghai 201800 China

Rapid electron transfer from electron adduct and reducing OH adduct of 2-deoxyadenosine-5′-monophosphate acid (dAMP) to riboflavin (RF) and flavin adenine dinucleotide (fad) was studied using the time-resolved pulse radiolysis techniques. Both spectroscope and kinetic analyses showed that transient absorption spectrum of electron adduct or OH adduct of dAMP formed at first, and men changed to that of radical anion of RF or fad after several microseconds of pulse. The evidence indicated that electron transfer from electron adduct and reducing OH adduct of dAMP to RF or fad did occur. From buildup or bleaching kinetics of radical anions of RF and fad, the rate constants for electron transfer were determined, respectively.

关键词： Electron adduct of dAMP reducing OH adduct of dAMP riboflavin (RF) fad electron transfer pulse radiolysis

亚油酸与核黄素或fad激发态之间电荷转移的直接证据

在线全文

学校读者我要写书评

暂无评论

化学学报 2001年第11期59卷 1858-1863页

作者：陆长元刘官树韩镇辉唐红飞姚思德林念芸陈君男毛裕民中国科学院上海原子核研究所复旦大学遗传所

根据荧光显微镜方法,我们首次发现核黄素(维生素B2)主要分布在细胞核的膜上和核的内部,故核黄素光敏化与辐射敏化的靶位置主要集中在细胞核内;当核黄素的浓度较大时,细胞膜上也有药物的分布,即在高浓度时,细胞膜也是光敏化与辐射敏化的... 详细信息

根据荧光显微镜方法,我们首次发现核黄素(维生素B2)主要分布在细胞核的膜上和核的内部,故核黄素光敏化与辐射敏化的靶位置主要集中在细胞核内;当核黄素的浓度较大时,细胞膜上也有药物的分布,即在高浓度时,细胞膜也是光敏化与辐射敏化的作用位点之一.应用308nm激光光解时间分辨吸收方法,以亚油酸作为脂质的模型化合物,研究了亚油酸与核黄素和黄素腺嘌呤二核苷酸(fad)的激发三重态之间的电荷转移过程,首次给出了电荷转移的直接证据.

关键词：亚油酸核黄素 DNA 辐射损伤光敏化黄素腺嘌呤二核苷酸荧光显微镜电荷转移 fad 激发态

fad为辅基的葡萄糖脱氢酶发酵、纯化及酶学性质

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

食品与生物技术学报 2020年第2期39卷 104-111页

作者：林荣宋祖坤张玲王男杨海麟江南大学工业生物技术教育部重点实验室江苏无锡214122

细菌来源的fad为辅基的葡萄糖脱氢酶(fad-GDH)较为罕见,作者利用乳糖为诱导剂,发酵*** BL21(DE3)生产fad-GDH。7.5 L发酵罐中培养该菌,通过分批补料,分阶段温度控制,酶活达到1341 U/L,菌体量达12.4 g/L。通过镍柱、脱盐柱、阴离子交换... 详细信息

细菌来源的fad为辅基的葡萄糖脱氢酶(fad-GDH)较为罕见,作者利用乳糖为诱导剂,发酵*** BL21(DE3)生产fad-GDH。7.5 L发酵罐中培养该菌,通过分批补料,分阶段温度控制,酶活达到1341 U/L,菌体量达12.4 g/L。通过镍柱、脱盐柱、阴离子交换柱三步层析对粗酶液分离纯化,获得比酶活109 U/mg的重组酶。SDS-PAGE凝胶电泳显示,重组蛋白质纯化后呈单一条带,相对分子质量约60000。等电聚焦电泳检测酶的等电点pI为5.3。酶标仪全波长扫描吸收光谱表明,该酶的辅基为fad,与酶非共价键结合。相比其它糖类,以葡萄糖为底物酶的Km最小,为2.56 mmol/L,kcat/Km为3487.7 L/(mmol·s)。该酶在pH 5.5~8.0内稳定,最适反应pH 7.0,当pH高于8.0时酶活迅速降低。差式扫描量热分析(DSC)测得酶的Tm值为77℃,热稳定性良好。本研究为该酶的进一步工业化生产应用提供参考与借鉴。

关键词： fad 葡萄糖脱氢酶乳糖诱导分离纯化酶学性质

SAD和fad家族基因调控山核桃不饱和脂肪酸组分配比

在线全文

学校读者我要写书评

暂无评论

园艺学报 2018年第2期45卷 250-260页

作者：黄春颖黄有军吴建峰黄仁栾雨濛张深梅王正加张启香黄坚钦浙江农林大学省部共建亚热带森林培育国家重点实验室浙江临安311300

以山核桃胚为试材,测定5个山核桃油脂合成积累关键时期(授粉后75、85、100、109和127 d)的油脂组分和油含量,并利用实时荧光定量PCR检测山核桃Δ9–硬脂酰–ACP脱氢酶(SAD)基因(CcSAD-1、CcSAD-2、CcSAD-3)和山核桃脂肪酸去饱和酶(fad)... 详细信息

以山核桃胚为试材,测定5个山核桃油脂合成积累关键时期(授粉后75、85、100、109和127 d)的油脂组分和油含量,并利用实时荧光定量PCR检测山核桃Δ9–硬脂酰–ACP脱氢酶(SAD)基因(CcSAD-1、CcSAD-2、CcSAD-3)和山核桃脂肪酸去饱和酶(fad)基因(Ccfad2、Ccfad3-1、Ccfad3-2、Ccfad3-3、Ccfad6、Ccfad7、Ccfad8-1、Ccfad8-2、Ccfad8-3)的表达模式。结果表明:成熟的山核桃种仁含油率超过种仁干质量的70%,同时不饱和脂肪酸比例达到90%以上;山核桃5个时期的脂肪酸组分,主要由棕榈酸(C16︰0)、硬脂酸(C18︰0)、油酸(C18︰1)、亚油酸(C18︰2)以及亚麻酸(C18︰3)组成;随着山核桃SAD家族基因表达上升,山核桃油中的油酸含量上升并维持一定水平,其中CcSAD-3可能是影响油酸含量的主效基因;同时随着山核桃油酸含量上升,Ccfads基因表达上升,山核桃不饱和脂肪酸比例增加。

关键词：山核桃 SAD fad 油脂合成

Use of Quality Control Circles-A fad or Need

在线全文

学校读者我要写书评

暂无评论

Computer Aided Drafting,Design and Manufacturing 2006年第1期16卷 44-49页

作者： AMIR Manzur Wain SHAHID Ikramullah Butt Superior Group of Colleges Department of Business Administration 30-L Gulberg III Lahore Pakistan School of Mechanical and Vehicular Engineering Beijing Institute of Technology Beijing 100081 China

Different techniques including Quality Control Circles, Business Process Reengineering and Statistical Process Control （SPC）, Total Quality Management have been in use to reduce waste in the industry. Waste is considered as necessary evil and there are many types of waste in the industry. There are many ways and means to reduce waste but the less costly is to use Quality Control Circles technique effectively and efficiently. In quality control circles human resources that consist of small groups of employees are selected, who voluntarily meet at regular intervals to identify, analyze and solve quality and other problems in their work areas. In the Quality Control Circles different techniques of the quality improvement and total quality management are utilized which could be numeric or nonnumeric such as statistical quality control techniques and fish bone diagram or brain storming respectively. This article discusses that how reduction of waste in Pakistan industry is possible by using Quality Control Circles implementation.

关键词： Quality Control Circles (QCCs) fad waste reduction continuous improvement processes

Molecular dynamics simulations of a DNA photolyase protein: High-mobility and conformational changes of the fad molecule at low temperatures

在线全文

学校读者我要写书评

暂无评论

Advances in Bioscience and Biotechnology 2012年第3期3卷 169-180页

作者： Kholmirzo Kholmurodov Ermuhammad Dushanov Kenji Yasuoka Department of Mechanical Engineering Keio University Yokohama Japan Laboratory of Radiation Biology Joint Institute for Nuclear Research Dubna Russia

A molecular dynamics (MD) simulation is performed on a DNA photolyase to study the conformational behavior of the photoactive cofactor flavin adenine dinucleotide (fad) inside the enzyme pocket. A DNA photolyase is a highly efficient light-driven enzyme that repairs the UV-induced cyclobutane pyrimidine dimer in damaged DNA. In this work, the fad conformational and dynamic changes were studied within the total complex structure of a DNA photolyase protein (containing fadH–, MTHF, and DNA molecules) embedded in a water solvent. We aimed to compare the conformational changes of the fad cofactor and other constituent fragments of the molecular system under consideration. The obtained results were discussed to gain insight into the light-driven mechanism of DNA repair by a DNA photolyase enzyme—based on the enzyme structure, the fad mobility, and conformation shape.

关键词： Molecular Dynamics Simulations DNA Photolyase Conformation Change fad MTHF DNA

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Rental fad

China Today 2011年第10期60卷 59-61页

作者： ZHU HONG

SU Wei is on a business trip to Beijing. She rents a car and drives herself to the places she needs to go."It is more convenient than taking a taxi,"says Su, who is one of the growing number of young Chinese who prefe... 详细信息

关键词：出租车 fad 中国人汽车

Mutations at Cys392 and Asp393 influence the fad Binding and the Formation and Stability of the Radical State of Chlamydomonas reinhardtii Cryptochrome

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Mutations at Cys392 and Asp393 influence the FAD Binding and...

fad定量荧光检测分析仪研制及其在地质录井中的应用

中国生物化学与分子生物学会第十二届全国会员代表大会暨2018年全国学术会议

作者： Lei Xu Bin Wen Wengui Shao Qianwu Huang Jun Lv Yao Zhang Guoping Zhu Anhui Province Key Laboratory of Active Biological Macro-molecules Wannan Medical College Institute of Molecular Biology and Biotechnology Anhui Normal University

Plant cryptochromes are blue-light receptors that regulate the light-dependent growth,development and circadian rhythm in *** fad cofactor is bound in the photolyase homology region(PHR) of plant cryptochromes that will be photoreduced to the neutral radical state under blue *** photoreaction may trigger conformational changes in the PHR and the C-terminal extension(CCT) of plant cryptochromes,and lead to the subsequent signal *** unicellular green alga Chlamydomonas reinhardtii contains a single plant cryptochrome,named Chlamydomonas photolyase homologue 1(CPH1).In CPH1,Cys392 and Asp393 is located near the isoalloxazine moiety of the fad *** we demonstrated that replacing Cys392 in CPH1 with Ser or Asn had little effect on the binding of fad and the formation of the neutral radical under blue *** the neutral radical in the C392 N mutant was oxidized in a significantly lower rate than that in CPH1 and the C392 S *** contrast,substituting Asn for Asp393 in CPH1 improved the binding affinity for fad as well as the stability of the neutral radical;but the radical formation in the mutant was severely *** results reveal that the residues at site 392 may tune the stability of the neutral radical,and the Asp at site 393 is crucial for the formation of the radical in plant cryptochromes.

关键词： plant cryptochrome fad photoreduction mutation radical

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

江汉石油学院学报 2000年第4期22卷 38-41页

作者：李健李一超曹洪辉中原石油勘探局勘探开发科学研究院河南濮阳457001 中原石油勘探局地质录井处河南濮阳457001

阐述了新研制的 fad定量荧光检测分析仪的工作原理 ,介绍了该仪器在肉眼不可见荧光的探测、地层荧光及泥浆污染的识别与排除以及

关键词：油气勘探 fad 定量荧光检测分析仪荧光录井

在线全文

学校读者我要写书评

暂无评论

基于fad的UML活动图测试用例生成

内蒙古大学学报（自然科学版） 2011年第1期42卷 94-100页

作者：田永红苏依拉内蒙古工业大学信息工程学院呼和浩特010051

在软件系统的设计过程或文档说明中,软件系统的动态行为可通过UML活动图描述,软件系统的正确性与可靠性要通过构造一定的测试模型来验证.由于UML活动图缺乏精确的数学模型描述,所以无法直接生成有效的测试模型.本文研究将UML活动图模型... 详细信息

在软件系统的设计过程或文档说明中,软件系统的动态行为可通过UML活动图描述,软件系统的正确性与可靠性要通过构造一定的测试模型来验证.由于UML活动图缺乏精确的数学模型描述,所以无法直接生成有效的测试模型.本文研究将UML活动图模型与fad(形式活动图)相结合,应用fad的片段方程式理论详细分析了生成测试用例的完整过程.其结果在软件测试中具有一定的理论指导意义和应用价值.

关键词： fad UML 活动图软件测试