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Journal of Medical Colleges of PLA(China) 2000年第2期15卷 131-134页

作者：夏炜郭树忠鲁开化

Objective: To analyze and compare the expression pattern of the transforming growth factor-β1(TGF-β1) and its type I receptor (TGF-β RI ) in nounal human skin and various phases of post-burn hypertrophic scars (HTS). Method: The immunohistochemical ABC method was employed. Results: In nounal human skin, no evident immunoreactivity of TGF-β1 and TGF-β R I was observed. In activation phase of post-burn HTS, TGF-β R I and TGF-β1 were highly expressed in most dermal fibroblasts which seemed to be the same subset. However, in remission phase, no staining was seen in der mal fibroblasts. Conclusion: The formation of all may involve the increase of TGF-β responsiveness in fibroblasts The ac cumulation at the wound site and failure of apoptosis of over-resposive fibroblasts may contribute to the formation of HTS.

关键词： hypertrophic scar transforming growth factor-β1 transforming growth factor-β receptor I immunohistochemistry

Expression of c-erbB-2 oncogene protein, epidermal growth factor receptor, and TGF-β₁ in human pancreatic ductal adenocarcinoma

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Hepatobiliary & Pancreatic Diseases International 2002年第4期1卷 620-623页

作者： Li Zhang Shi-Zhen Yuan Second Affiliated Hospital Nanhua University Hengyang 421001 China. geyuha8@***

Objective: To detect the relations of c-erbB-2 onco-gene protein, epidermal growth factor receptor (EG-FR) and transforming growth factor-β1 (TGF-β1)to the progression or metastasis of pancreatic ***: Using streptavidinbiotin complex (SABC)method, c-erbB-2 oncongene protein, we examinedimmunohistochemically EGFR and TGF-β1 expres-sions in wax-tissue sections from 10 individuals withnormal pancreas (NP), 13 patients with chronic pan-creatitis (CP) and 36 patients with pancreatic ductaladenocarcinoma (PC).Results: The positive expression rates of c-cerbB-2oncogene protein, EGFR and TGF-β1 in the NP, CPand PC groups were 0, 0, 10%; 7.7%, 7.7%,7.7%; and 41.7%, 50.0%, 44.4%, *** positive expression rates of the three specific pro-teins increased more significantly in the PC groupthan in the NP and CP groups (P0.05), but to the clinical stage of tumors(P<0.01). The coexpression rate of the three pro-teins was 27.8 % (10/36). This coexpression in thePC group was correlated with the histopathologicalgrades and clinical stages of tumors (P<0.01).Conclusion: Detection of c-erbB-2 oncogene protein,EGFR, and TGF-β1 expressions in pancreatic tissueis helpful to judge the malignancy, progression, andmetastasis of PC.

关键词： pancreatic neoplasms proto-oncogene proteins c-erbB-2/AN receptors epidermal growth factor receptor transforming growth factor-β1

二黄汤对哮喘模型大鼠肺组织中TGF-β_1及体内IL-33的影响

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天津医药 2014年第4期42卷 337-340页

作者：聂艳辉霍博雅孙会珍辽宁医学院辽宁锦州121000 辽宁医学院附属第三医院中医科

目的观察二黄汤对哮喘模型大鼠肺组织中转化生长因子β1(TGF-β1)及体内白介素-33(IL-33)的影响。方法 50只SD大鼠随机均分为正常组、哮喘组、布地奈德组、高剂量二黄汤组(含生药量68 g/kg)和低剂量二黄汤组(含生药量17 g/kg)。以卵清... 详细信息

目的观察二黄汤对哮喘模型大鼠肺组织中转化生长因子β1(TGF-β1)及体内白介素-33(IL-33)的影响。方法 50只SD大鼠随机均分为正常组、哮喘组、布地奈德组、高剂量二黄汤组(含生药量68 g/kg)和低剂量二黄汤组(含生药量17 g/kg)。以卵清白蛋白致敏与激发建立哮喘大鼠模型,随后分别用布地奈德、二黄汤干预治疗。肺组织切片HE染色观察病理变化并检测气道管壁厚度(Wat)及气道平滑肌厚度(Wam),用免疫组化法检测肺组织TGF-β1蛋白的表达,酶联免疫法检测血清及支气管肺泡灌洗液(BALF)中IL-33的含量。结果所有药物干预组较哮喘组炎症细胞浸润明显减轻;布地奈德组、高剂量二黄汤组和低剂量二黄汤组Wat均较哮喘组下降(μm2/μm:54.99±8.82、52.28±7.61、58.53±7.63 vs 79.50±5.64,P1.72、21.20±4.50 vs 30.16±1.68,P1高于正常组(IOD:12.60±2.25 vs 1.67±0.17),布地奈德组(5.51±2.48)、高剂量二黄汤组(5.22±2.52)和低剂量二黄汤组(6.92±2.18)均低于哮喘组(P1、IL-33呈正相关。结论二黄汤可在一定程度上干预哮喘大鼠气道重塑,其作用可能是通过调节TGF-β1和IL-33实现的。

关键词：哮喘气道重塑二黄汤转化生长因子-β1 白细胞介素-33 transforming growth factor-β1 leukotriene-3

Hypermethylation of TGF-β1 gene promoter in gastric cancer

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World Journal of Gastroenterology 2013年第33期19卷 5557-5564页

作者： Yong-Qi Wang Yu-Min Li Xun Li Tao Liu Xiao-Kang Liu Jun-Qiang Zhang Ju-Wu Guo Ling-Yun Guo Liang Qiao School of Life Sciences Lanzhou University Department of Anesthesiology the First Hospital of Lanzhou University Key Laboratory of Digestive System Tumors General Surgery the Second Hospital of Lanzhou University General Surgery the First Hospital of Lanzhou University Storr Liver Unit at the Westmead Millennium Institute the University of Sydney

AIM:To examine transforming growth factor-β1(TGF-β1)promoter methylation in gastric cancer and to determine if Helicobacter pylori(***)or interleukin(IL)-1β could induce TGF-β1 hypermethylation in ***:We examined the frequency and extent of TGF-β1 promoter methylation using methylationspecific PCR in the gastric tissues from 47 gastric cancer patients and 39 non-gastric cancer *** infection was confirmed by a positive result from either a serological test,histological analysis or C13urea breath ***-1 and MKN-45 cells co-cultured with *** or treated with IL-1β for 12,24 and 48 h in vitro tested the effects of *** or IL-1β on TGF-1β.RESULTS:Twenty-four/forty-seven(51%)cases of gastric cancer(GC)tissues showed TGF-β1 promoter methylation,15/47(31.9%)cases of matched noncancerous gastric mucosa tissues from the GC patients,and 11/39(28%)case of the normal gastric mucosa tissues from non-GC subjects showed TGF-β1 promoter methylation(51%vs 28%,P1 were found in the tumor tissues than in non-tumor tissues from GC patients(0.24±0.06 vs 0.17±0.04,P15±0.03,P1 methylation was found in 48.3% of ***-positive gastric mucosal tissues whereas only 23.1% of ***-negative gastric mucosal tissues showed TGF-β1 methylation(48.3%vs 23.1%,P1β appeared to induce a dose-dependent methylation of TGF-β1 and the strongest methylation was observed in GES-1 cells treated with 2.5 ng/mL of IL-1β for 48 *** studies showed that pre-treatment of GES-1 cells with 20ng/mL IL-1RA for 1 h could partially abolish the effect of IL-1β on TGF-β1 *** of GES-1cells by *** was not found to induce significant TGF-β1 promoter ***:Our data revealed that TGF-1 promoter is methylated in GC ***-1β may be an important mediator for *** induced gene methylatio

关键词： Transforming growth factor-β1 Interleukin-1β Methylation Helicobacter pylori Gastric cancer

Endoscopic resection of the pancreatic tail and subsequent wound healing mechanisms in a porcine model

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World Journal of Gastroenterology 2019年第21期25卷 2623-2635页

作者： Sheng Wang Kai Zhang Jin-Long Hu Wei-Chao Wu Xiang Liu Nan Ge Jin-Tao Guo Guo-Xin Wang Si-Yu Sun Endoscopy Center Shengjing Hospital of China Medical University

BACKGROUND Laparoscopic resection of the pancreatic body and tail is the predominant methodology to remove lesions in these locations;its safety and surgical planning are relatively mature, but it remains a complex and high-precision surgical operation, requiring abundant experience and skills in laparoscopic surgery, with a 10% rate of complications. AIM To verify the feasibility and safety, as well as to examine the complications of endoscopic pancreatectomy and healing mechanisms of pancreatic wounds after endoscopic resection. METHODS Transgastric endoscopic resections of varying sizes of pancreases were performed in 15 healthy Bama miniature pigs. The technical success rate, the incidence of serious complications, and the survival of the animals were studied. The healing of the wounds was evaluated by sacrificing the animals at various time points. Finally, the expression of transforming growth factor-β1 and Smad3/Smad7 in the surgical site was examined by immunohistochemistry to explore the role of these factors in wound healing of the pancreas. RESULTS Partial and total resections were successfully performed in two groups of animals, respectively. The technical success rate and the survival rate of the pigs were both 100%. We obtained 12 pancreatic tissue samples by endoscopic resection. The pancreatic wounds were closed with metal clips in one group and the wounds healed well by forming scars. There was a small amount of pancreatic leakage in the other group, but it can be fully encapsulated. The level of transforming growth factor-β1 (TGF-β1) in the wounds increased during the inflammatory and fibrous hyperplasia phases, and decreased in the scar phase. The expression of Smad3 paralleled that of TGF-β1, while the expression of Smad7 had an inverse relationship with the expression of TGF-β1. CONCLUSION Purely transgastric endoscopic resection of the pancreas is a safe, effective, and feasible procedure, but the incidence of pancreatic leakage in total pancre

关键词： Pancreatic resection Endoscopy Wound healing Transforming growth factor-β1 Smad

Aberrant TGF-β1 signaling contributes to the development of primary biliary cirrhosis in murine model

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World Journal of Gastroenterology 2013年第35期19卷 5828-5836页

作者： Bin Liu Xuan Zhang Feng-Chun Zhang Jin-Bao Zong Wen Zhang Yan Zhao Department of Rheumatology theAffiliated Hospital of Qingdao University Medical College Department of Rheumatology Peking Union Medical College Hospital Chinese Academy of Medical Science

AIM:To investigate whether transforming growth factor-β1(TGF-β1)signaling pathway is involved in the pathogenesis of primary biliary cirrhosis(PBC).METHODS:A murine model of PBC was developed by injection of polyinosinic polycytidylic acids(polyⅠ:C)in C57BL/6 mice,and the liver expressions of TGFβ1,TGF-βreceptorⅠ(TβRⅠ),TGF-βreceptorⅡ(TβRⅡ),p-Smad2/3,monoclonalα-smooth muscle actin antibody(α-SMA)andα1(Ⅰ)collagen in the mouse model and control mice were evaluated by immunohistochemistry,immunoblotting and real-time polymerase chain reaction(RT-PCR).Lymphocyte subsets in liver were analyzed using flow ***:The mouse model had several key phenotypic features of human PBC,including elevated levels of alkaline phosphatase,antimitochondrial antibodies,portal bile ducts inflammation,and progressive collagen *** with control mice,protein and mRNA levels of TGFβ1,TβRⅠ,TβRⅡ,p-Smad2/3,α-SMA andα1(Ⅰ)collagen in liver(1.7±0.4 vs 8.9±1.8,0.8±0.2 vs 5.1±1.5,0.6±0.01 vs5.1±0.1,0.6±0.3 vs 2.0±0.3,0.9±0.4 vs 3.4±0.6,0.8±0.4 vs 1.7±0.3,1.1±1.2 vs 11.8±0.6,P1±0.001vs 0.004±0.00,0.12±0.04 vs 0.52±0.23,P1)were higher in the mouse ***:TGFβ1 might play a dual role in the development of PBC:it suppresses inflammatory response but operates to enhance *** aberrant activity of TGF-β1 signaling contributes to the development of PBC.

关键词： Primary biliary cirrhosis Transforming growth factor-β1 Regulatory T cell Liver

Association of CT perfusion imaging with plasma levels of TGF-β1 and VEGF in patients with NSCLC

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Asian Pacific Journal of Tropical Medicine 2016年第2期9卷 175-177页

作者： Da-Wei Li Bao-Zhong Wu Yu-Sen Shi Zhi-Qun Li Xu-Dong Liu Xiao-Hua Li Radiology Department Affiliated Hospital of Hainan Medical College

Objective: To study the association of CT perfusion imaging parameters with plasma level of transforming growth factor-β1(TGF-β1) and vascular endothelial growth(VEGF) in patients with non small cell cancer(NSCLC). Methods: A total of 67 patients with NSCLC(NSCLC group) and 64 patients with benign lesion(control group) were given with CT perfusion imaging to obtain blood flow, blood volume, mean transit time, time to peal and permeability surface through CT perfusion software. The plasma levels of TGF-β1 and VEGF were tested by ELISA. The relationship between plasma levels of TGF-β1, VEGF and CT perfusion imaging parameters were analyzed. Results: CT perfusion imaging parameters and the plasma levels of TGF-β1 and VEGF of NSCLC group were significantly higher than the control group(P1 and VEGF in NSCLC group showed significant difference in different tumor node metastasis stages(P1 and VEGF were positively correlated with blood flow, blood volume, and mean transit time(P1 and VEGF with the permeability surface. Conclusions: CT perfusion imaging parameters in patients with NSCLC is closely associated with plasma TGF-β1, VEGF and its biological characteristics. CT perfusion imaging is a convenient method to detect tumor blood perfusion.

关键词： Non small cell lung cancer CT perfusion parameters Transforming growth factor-β1 Vascular endothelial growth

Role of IL-10 and TGF-β1 in local immunosuppression in HPV-associated cervical neoplasia

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World Journal of Clinical Oncology 2014年第4期5卷 753-763页

作者： Kirvis Torres-Poveda Margarita Bahena-Román Claudia Madrid-González Ana I Burguete-García Víctor Hugo Bermúdez-Morales Oscar Peralta-Zaragoza Vicente Madrid-Marina Chronic Infection and Cancer Division Research Center on Infectious Diseases Instituto Nacional de Salud Pública 62100 Cuernavaca Morelos Mexico

Cervical cancer is a worldwide disease that constitutes a significant public health problem, especially in developing countries, not only due to its high incidence but also because the most affected population comprises women who belong to marginalized socio-economic classes. Clinical and molecular research has identified immunological impairment in squamous intraepithelial cervical lesions and cervical cancer patients. Human Papillomavirus(HPV) has several mechanisms for avoiding the immune system: it down-regulates the expression of interferon and upregulates interleukin(IL)-10and transforming growth factor(TGF)-β1 to produce a local immunosuppressive environment, which, along with altered tumor surface antigens, forms an immunosuppressive network that inhibits the antitumor immune response. In this review we analyzed the available data on several deregulated cellular immune functions in patients with NIC Ⅰ, NIC Ⅱ and NIC Ⅲ and cervical cancer. The effects of immunosuppressive cytokines on innate immune response, T-cell activation and cellular factors that promote tumor cell proliferation in cervical cancer patients are summarized. We discuss the functional consequences of HPV E2, E6, and E7 protein interactions with IL-10 and TGF-β1 promoters in the induction of these cytokines and postulate its effect on the cellular immune response in squamous intraepithelial cervical lesions and cervical cancer patients. This review provides a comprehensive picture of the immunological functions of IL-10 and TGF-β1 in response to HPV in humans.

关键词： Cervical cancer Immunosuppression Interleukin-10 Transforming growth factor-β1 Human Papillomavirus

慢性心力衰竭患者血清转化生长因子-β_1和结缔组织生长因子水平与心功能关系

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疑难病杂志 2014年第3期13卷 224-226页

作者：王评夏裕郑壁伟夏清华广东省清远市中医院心血管内科 511500

目的观察慢性心力衰竭(CHF)患者血清转化生长因子-β1(TGF-β1)和结缔组织生长因子(CTGF)的变化及与心功能相关性。方法收集105例CHF患者为CHF组,以68例健康查体者为健康对照组。检测血清TGF-β_1、CTGF水平及左心室射血分数(LVEF)、左... 详细信息

目的观察慢性心力衰竭(CHF)患者血清转化生长因子-β1(TGF-β1)和结缔组织生长因子(CTGF)的变化及与心功能相关性。方法收集105例CHF患者为CHF组,以68例健康查体者为健康对照组。检测血清TGF-β_1、CTGF水平及左心室射血分数(LVEF)、左心室质量指数(LVMI)变化,进行相关分析。结果 CHF患者血清TGF-β_1、CTGF均显著高于健康对照组(P均1);TGF-β_1、CTGF升高与心力衰竭严重程度相平行;TGF-β_1、CTGF均与LVEF呈负相关(r=-0.638,P1;r=-0.743,P1),与LVMI(r=0.630,P1;r=0.748,P1)、心功能分级(r=0.647,P1;r=0.753,P1)呈正相关。结论血清TGF-β_1、CTGF共同参与了CHF的病理生理过程;血清CTGF水平可作为诊断及判断心力衰竭严重程度的生物学指标。

关键词：心力衰竭转化生长因子-β1 结缔组织生长因子 Transforming growth factor-β1

缺氧诱导因子1α和转化生长因子β1在子宫内膜癌中的表达及其临床意义

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中国综合临床 2016年第9期32卷 830-833页

作者：杨军文倪观太皖南医学院弋矶山医院妇产科安徽省芜湖市241001

目的：检测子宫内膜癌组织中缺氧诱导因子1α（ HIF-1α）和转化生长因子β1（ TGF-β1）的表达并探讨其临床意义。方法选取2012年2月至2015年2月手术治疗54例子宫内膜癌及20例正常子宫内膜，应用免疫组化法检测HIF-1α和TGF-β1的表达... 详细信息

目的：检测子宫内膜癌组织中缺氧诱导因子1α（ HIF-1α）和转化生长因子β1（ TGF-β1）的表达并探讨其临床意义。方法选取2012年2月至2015年2月手术治疗54例子宫内膜癌及20例正常子宫内膜，应用免疫组化法检测HIF-1α和TGF-β1的表达，并分析其与临床病理及预后的关系。结果子宫内膜癌组织中HIF-1α和TGF-β1表达分别为59．26％（32／54）、64．81％（35／54），二者显著高于正常子宫内膜癌组织（1／20、0）（χ2值分别为17．390、24．596，P均＜0．01）；HIF-1α表达与子宫内膜癌FIGO分期（χ2＝6．712，P＝0．010）、脉管浸润（χ2＝4．804，P＝0．036）、浸润深度（χ2＝6．484，P＝0．011）及腹膜后淋巴结转移（χ2＝5．732， P＝0．017）均相关；TGF-β1表达与子宫内膜癌 FIGO 分期（χ2＝4．878， P＝0．027）、浸润深度（χ2＝4．060，P＝0．044）及腹膜后淋巴结转移（χ2＝5．211，P＝0．022）；在子宫内膜癌组织HIF-1α和TGF-β1表达呈正相关（ r＝0．705，P＝0．000）。结论 HIF-1α和TGF-β1增高可能共同参与了宫内膜癌发生及浸润转移，并有可能成为其治疗的靶点。

关键词：子宫内膜癌缺氧诱导因子1α 转化生长因子β1 Hypoxia inducible factor-1α Transforming growth factor-β1