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Differentially expressed genes identified by cross-species microarray in the blind cavefish Astyanax

Integrative Zoology 2009年第1期4卷 99-109页

作者： Allen G.STRICKLER William R.JEFFERY Department of Biology University of MarylandCollege ParkUSA

Changes in gene expression were examined by microarray analysis during development.of the eyed surface dwelling(surface fish)and blind cave-dwelling(cavefish)forms of the teleost Astyanax mexicanus De Filippi,*** cross-species microarray used surface and cavefish RNA hybridized to a DNA chip prepared from a closely related species,the zebrafish Danio rerio Hamilton,*** identified a total of 67 differentially ex-pressed probe sets at three days post-fertilization:six upregulated and 61 downregulated in cavefish relative to surface *** of these genes function either in eye development.and/or maintenance,or in programmed cell *** upregulated probe set showing the highest mean fold change was similar to the human ubiquitin specific protease 53 *** downregulated probe sets showing some of the highest fold changes corresponded to genes with roles in eye development.including those encoding gamma crystallins,the guanine nucleotide binding pro-teins Gnat1 and Gant2,a BarH-like homeodomain transcription factor,and *** of gam-ma-crystallin and rhodopsin was confirmed by in situ hybridization and immunostaining with specific *** downregulated genes encode molecules that inhibit or activate programmed cell *** results suggest that cross-species microarray can be used for identifying differentially expressed genes in cavefish,that many of these genes might be involved in eye degeneration via apoptotic processes,and that more genes are downregulated than upregulated in cavefish,consistent with the predominance of morphological losses over gains during regressive evolution.

关键词： apoptosis blind cavefish cross-species microarray differentially expressed genes eye development.

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