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PD-L1 is increased in the spinal cord and infiltrating lymphocytes in experimental allergic encephalomyelitis

Neural Regeneration Research 2013年第35期8卷 3296-3305页

作者： Min Li Jiandong Jiang Bing Fu Jiechun Chen Qun Xue Wanli Dong Yanzheng Gu Lingtao Tang Limin Xue Qi Fang Mingyuan Wang Xueguang Zhang Department of Neurology Second People's Hospital of Lianyungang City Department of Neurology First Affiliated Hospital of Soochow University Institute of Clinical Immunology Soochow University Department of Neurology Third Hospital of Xingtai City Red-Cross Blood Center of Suzhou City

experimental allergic encephalomyelitis is a mouse model of human multiple sclerosis with similar pathology and pathogenesis. Thl cells play an important role in the pathogenesis of experimental allergic encephalomyelitis. This study determined the potential effect of programmed cell death 1 ligand 1 in the pathogenesis of experimental allergic encephalomyelitis induced by injecting myelin oligodendrocyte glycoprotein, complete Freund＇s adjuvant and Bordetella pertussis toxin into C57BL/6J mice. experimental allergic encephalomyelitis mice developed disease and showed in- flammatory changes in the central nervous system by hematoxylin-eosin staining of spinal cord pathological sections, demyelination by Luxol fast-blue staining and clinical manifestations. The expression of programmed cell death 1 ligand 1 in mice was detected by immunohistochemistry, flow cytometry and western blot anatysis. The expression of programmed cell death 1 ligand 1 in the spinal cord and splenocytes of mice was significantly increased compared with normal mice. Our findings suggest the involvement of programmed cell death 1 ligand 1 in the pathogenesis of ex- perimental allergic encephalomyelitis and suggest this should be studied in multiple sclerosis.

关键词： neural regeneration experimental allergic encephalomyelitis multiple sclerosisautoimmune disease costimulatory signal costimulatory molecule programmed ce1 B7-CD28 superfamily grants-supported paper neuroregenerationanimal models II death 1 ligand

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Effect of olfactory ensheathing cell transplantation on myelin repair and motor function in a rat model of experimental allergic encephalomyelitis

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Neural Regeneration Research 2009年第4期4卷 281-285页

作者： Yifeng Du Shougang Guo Feng Jin Minzhong Wang Gongming Wang Department of Neurology Provincial Hospital Affiliated to Shandong University Jinan 25002 1 Shandong Province China Department of Neurosurgery Affiliated Hospital of Jining Medical University Jining 272029 Shandong Province China Department of Anesthesiology Provincial Hospital Affiliated to Shandong University Jinan 250021 Shandong Province China

BACKGROUND： Olfactory ensheathing cell transplantation can activate axonal regeneration and enhance myelin repair, which are beneficial for treating demyelinating diseases. OBJECTIVE： To explore the effects of olfactory ensheathing cell transplantation on myelin repair, synaptophysin expression, and motor function in a rat model of experimental allergic encephalomyelitis. DESIGN, TIME AND SETTING： A randomized, controlled experiment was performed at the Laboratory of Provincial Hospital affiliated to Shandong University between August 2006 and September 2007. MATERIALS： Dibenzylamine （Hoechst 33342）, luxol fast blue, and rabbit anti-rat synaptophysin antibody were provided by Sigma, USA. METHODS： Olfactory ensheatbing cells extracted from neonatal Wistar rats were cultured for 10-14 days and labeled with dibenzylamine. Spinal cord extracted from a healthy guinea pig was homogenized and equally mixed with complete Freund＇s adjuvant; thereafter, the mixture was intracutaneously injected into two posterior voix pedis of healthy male Wistar rats to establish models of experimental allergic encephalomyelitis. Rats were randomly divided into a control encephalomyelitis group and an olfactory ensheathing cell transplantation group, 36 rats in each group. Physiological saline （2 μ L） or an olfactory ensheathing cell suspension （2 μ L） was separately injected along lateral cerebral ventricle at day 7 post-model induction. MAIN OUTCOME MEASURES： The migration and distribution of olfactory ensheathing cells were observed under fluorescence microscopy; myelin repair was detected using hematoxylin-eosin staining and luxol fast blue staining; synaptophysin expression was measured using immunohistochemical staining; motor function was evaluated using a motor function scale. RESULTS： Olfactory ensheatbing cells could survive in vivo and migrate to the distal end of the transplant focus and spinal cord, and survived 21 days. Hematoxylin-eosin staining and luxol fast blue staining in

关键词： olfactory ensheathing cells transplantation experimental allergic encephalomyelitis myelin synaptophysin

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Immunity phenomena following olfactory ensheathing cell transplantation into experimental allergic encephalomyelitis rat brain

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Neural Regeneration Research 2010年第15期5卷 1130-1137页

作者： Ainong Mei Jue Wang Qiong Cheng Xinqing Yang Jin Yang Pengli Zhu Shougang Guo Department of Cadre Clinic I Fujian Provincial Hospital Fuzhou 350001 Fujian Province China Department of Paediatrics Fujian Provincial Hospital Fuzhou 350001 Fujian Province China Department of Neurology Fujian Provincial Hospital Fuzhou 350001 Fujian Province China Department of Otolaryngology Fujian Provincial Hospital Fuzhou 350001 Fujian Province China Shandong Provincial Hospital Jinan 250000 Shandong Province China

Olfactory ensheathing cells （OECs） can promote axonal regeneration and remyelination for the treatment of spinal cord injury. OECs can also treat experimental allergic encephalomyelitis （EAE）, but it remains unclear whether OECs might be rejected by the immune system in the brain including the destruction of the blood-brain barrier under inflammation, the release of inflammatory factors, the activation of local antigen-presenting cells （e.g., microglia cells） and antigen drainage. We found that OECs expressed major histocompatibility complex （MHC）-I molecules on the cell surface, barely expressed MHC-II, but MHC-II could be induced by interferon-v, suggesting that OECs have certain immunogenicity. When OECs were transplanted into normal animal brains, no OECs were phagocytosed by dendritic cells in the cervical lymph node, and OECs did not induce lymphocyte proliferation, which indicates that OECs share some immune privilege under normal conditions. However, OECs in the rat EAE brain were phagocytosed by dendritic cells in the cervical lymph node and enhanced lymphocyte proliferation. These findings suggest that OECs are rejected because of increased immunogenicity in EAE brain, and that brain inflammation, in particular activated dendritic cells, may be a prerequisite for rejecting OECs.

关键词： olfactory ensheathing cells experimental allergic encephalomyelitis major histocompatibility complex dendritic cells transplantation immunity

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Effect of neuropeptide Y on white matter demyelination and serum interleukin-4 and gamma-interferon levels in the guinea pig with experimental allergic encephalomyelitis

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Neural Regeneration Research 2008年第5期3卷 554-557页

作者： Xiaohong Li Ke Yu Zuoxiao Li Department of Neurology Affiliated Hospital of Luzhou Medical College Luzhou 646000 Sichuan University China Department of Educational Administration Affiliated Hospital of Luzhou Medical College Luzhou 646000 Sichuan University China

BACKGROUND： Neuropeptide Y （NPY） may influence differentiation of Th cells immunological pathology of experimental allergic encephalomyelitis （EAE） is differentiation of Th cells It is assumed that the related to abnormal OBJECTIVE： To investigate the effect of NPY on white matter demyelination, the serum levels interleukin-4 （IL-4） and gamma-interferon （IFN-γ ）, as well as EAE pathogenesis in an EAE guinea pig model following NPY injection into the lateral cerebral ventricle. DESIGN, TIME AND SETTING： A randomized controlled animal study, which was performed in the Infection Immunity Animal Laboratory, Affiliated Hospital of Luzhou Medical College, China, from October 2005 to April 2006. MATERIALS： Thirty healthy female guinea pigs of 8-12 weeks of age, and 10 healthy female rats of three months of age were used. NPY was provided by Sigma Company, USA. NPY kit was provided by Beijing Huaying Biotechnology Institute, China. METHODS： Thirty guinea pigs were randomly divided into three groups： normal control group, EAE model group, and NPY intervention group （n =10 per group）. Normal control group and EAE model group： Saline （10μ L, once） was injected into the lateral cerebral ventricle. After one week, the same volume of Freund＇s adjuvant complete was either injected subcutaneously into two post-palms or EAE was modeled. NPY intervention group： EAE was modeled after one week and NPY was injected （10 μ L of 6 nmol NPY, once） into the lateral cerebral ventricle. Myelin basic protein （MBP） antigen made from rat spinal cord homogenate and Freund＇s adjuvant complete were injected subcutaneously into both post-palms （0.2 mL per palm） to establish the EAE model. MAIN OUTCOME MEASURES： White matter demyelination of the cerebrum, cerebellum, brain stem, and spinal cord were observed by light microscopy after HE staining. Levels of serum IFN-γ and IL-4 were detected by the double antibody sandwich ABC-ELISA technique. NPY content was detected by radioimmunoassay. RESULTS： Path

关键词： experimental allergic encephalomyelitis neuropeptide Y interleukin-4 gamma-interferon

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All-trans retinoic acid ameliorates experimental allergic encepha-lomyelitis by affecting dendritic cell and monocyte development

All-trans retinoic acid ameliorates experimental allergic en...

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中国神经科学学会第十届全国学术会议

作者： Xiao-Xia ZHAN Yu LIU Jin-Feng YANG Guang-You WANG Li-Li MU Tong-Shuai ZHANG Xiao-Li XIE Jing-Hua WANG Xin-Yue WANG Yu-Mei LIU Qing-Fei KONG Hu-Lun LI Bo SUN Department of Neurobiology Harbin Medical University Provincial Key Lab of Neurobiology Harbin Medical University

experimental allergic encephalomyelitis(EAE) can be elicited by injecting the MOG peptide *** cells(DCs) located at the immunization site phagocytize the MOG peptide,mature,migrate into the nearest draining lymph node(LN),and present antigen resulting in the activation of naive T *** and Thl7 cells represent the primary cells involved in EAE ***-trans retinoic acid(AT-RA) has been shown to have beneficial effects on EAE progression,however,whether AT-RA exerted its beneficial effect by influencing DC maturation or mediating other functions remains to be *** the present study,we showed that AT-RA lead to the down regulation of MHC-II,CD80(B7-1),and CD86(B7-2) expressed on the surface of DCs isolated from the draining LNs or spleen 3 days post immunization in an EAE *** to DC function influenced Th subset ***,the number of CD44 monocytes(which might trigger EAE progression) was also significantly decreased in draining LNs,spleen,subarachnoid space,and the spinal cord parenchyma post AT-RA *** findings demonstrated for the first time that the DC antigen presenting capacity was impaired by AT-RA resulting in the down regulation of pathogenic Thl and Thl7 inflammatory cell responses associated with reduced EAE severity.

关键词： All-trans retinoic acid dendritic cells experimental allergic encephalomyelitis

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细胞介导免疫在EAE发病中的作用

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中国免疫学杂志 1985年第2期 9-12页

作者：周建年杨贵贞白求恩医科大学微生物学教研室白求恩医科大学微生物学教研室长春

实验性变态反应性脑脊髓炎(简称EAE)是一种人工诱导的中枢神经系统自身免疫性疾病,也是自身免疫病的代表模式。关于EAE的免疫损伤机理,大多数学者认为致敏T细胞是引起EAE免疫损伤的主要原因;但也有报告指出:抗体也参与其损伤过程。有关... 详细信息

实验性变态反应性脑脊髓炎(简称EAE)是一种人工诱导的中枢神经系统自身免疫性疾病,也是自身免疫病的代表模式。关于EAE的免疫损伤机理,大多数学者认为致敏T细胞是引起EAE免疫损伤的主要原因;但也有报告指出:抗体也参与其损伤过程。有关这个问题的意见尚不统一。我们曾经在建立模型、提取髓磷脂硷蛋白(简称Bp)以及特异性免疫应答等方面做过一些工作。本文则以不同的实验手段和方法对EAE致病的主要原因作一系统观察,并对其可能的机理作出粗浅的分析。材料与方法一、动物及分组:豚鼠,由本校动物室供应。实验设有不同月龄组(4月龄,2 1/2月龄,1 1/2月龄)和新生期胸腺切除组,共4个组别,后者为2 1/2月龄,体重450g左右。各相应组别中,雌雄成比例分配。

关键词： Myelin Basic Protein experimental allergic encephalomyelitis Cell-Mediated Immunity

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