检索结果-南通市图书馆

separation of scleroglucan and cell biomass from Sclerotium glucanicum grown in an inexpensive, by-product based medium

引用

International Journal of Agricultural and Biological Engineering 2011年第1期4卷 52-60页

作者： Arlene Fosmer William Gibbons Biology&Microbiology Department South Dakota State UniversityBrookingsSouth Dakota 57007 USA

Scleroglucan is an extracellular polysaccharide produced by the fungi *** is stable over a broad range of temperatures,pH and salt concentration,thus having great potential in many diverse *** media for scleroglucan production contain expensive components,such as yeast extract,that make the medium cost-ineffective for some industrial *** we developed a medium which uses Condensed Corn Solubles(CCS),a nutrient-rich byproduct of corn-based ethanol production,to replace expensive *** typically used to recover scleroglucan are also expensive,and can limit *** evaluate alternative processes for scleroglucan recovery,we prepared scleroglucan in a modified version of Wang’s medium and a CCS-glucose *** samples were initially subjected to the standard recovery method to develop a complete mass balance,and then we evaluated various treatments to improve scleroglucan *** included heat treatments to lyse cells,using different alcohol precipitants,freezing or refrigerating before recovery,and diluting broth to enhance cell *** CCS medium produced 14.2 g/L scleroglucan,compared to 10.1 g/L in the modified Wang’s *** on the standard recovery protocol,we determined that 96%of the scleroglucan was recovered from the initial centrifugation and *** the cell pellet with water and recentrifuging only recovered a minimal amount of scleroglucan,and thus it could be eliminated from protocol to save energy and *** cells by boiling or autoclaving did not release more scleroglucan than un-heated samples,and we also found no statistical difference between ethanol,isopopanol,and methanol as scleroglucan *** the broth prior to scleroglucan recovery had no significant effect,while freezing actually decreased scleroglucan *** diluting the broth by 0.50 or 0.34 resulted in the greatest scleroglucan recovery,while

关键词： scleroglucan Sclerotium glucanicum exopolysaccharide separation condensed corn solubles

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：