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The expression of Spodoptera exigua P450 and UGT genes: tissue specificity and response to insecticides

Insect Science 2019年第2期26卷 199-216页

作者： Bo Hu Shu-Heng Zhang Miao-Miao Ren Xiang-Rui Tian Qi Wei David Kibe Mburu Jian-Ya Su Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education) College of Plant Protection Nanjing Agricultural University Nanjing China

Cytochrome P450 and UDP-glucosyltransferase (UGT) as phase I and phase II metabolism enzymes, respectively, play vital roles in the breakdown of endobiotics and xenobiotics. Insects can in crease the expression of detoxificatio n enzymes to cope with the stress from xenobiotics including insecticides. However, the molecular mechanisms for insecticide detoxification in Spodoptera exigua remain elusive, and the genes conferring insecticide metabolisms in this species are less well reported. In this study, 68 P450 and 32 UGT genes were identified. Phylogenetic analysis showed gene expansions in CYP3 and CYP4 clans of P450 genes and UGT33 family of this pest. P450 and UGT genes exhibited specific tissue expression patterns. Insecticide treatments in fat body cells of S. exigua revealed that the expression levels of P450 and UGT genes were significantly influenced by challenges of abamectin, lambda-cyhalothrin, chlorantraniliprole, metaflumizone and indoxacarb. Multiple genes for detoxification were affected in expression levels after insecticide exposures. The results demonstrated that lambda-cyhalothrin, chlorantraniliprole, metaflumizone and indoxacarb induced similar responses in the expression of P450 and UGT genes in fat body cells;eight P450 genes and four UGT genes were co-up-regulated significantly, and no or only a few CYP/UGT genes were down-regulated significantly by these four insecticides. However, abamectin triggered a distinct response for P450 and UGT gene expression;more P450 and UGT genes were down-regulated by abamectin than by the other four compounds. In con elusion, P450 and UGT genes from S. exigua were identified, and different responses to abamectin suggest a different mechanism for insecticide detoxification.

关键词： abamectin cytochrome P450 monooxygenases gene expression insecticide Spodoptera exigua UDP-glycosyltransferase

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Molecular characterization and developmental expression of the gene encoding the prothoracicotropic hormone in the beet armyworm, Spodoptera exigua

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Science China(Life Sciences) 2007年第4期50卷 466-472页

作者： XU Jun1,SU JianYa3, SHEN JinLiang3 & XU WeiHua1,2 1 Department of Molecular and Cell Biology, School of Life Sciences, University of Science and Technology of China, Hefei 230027, China 2 State Key Laboratory of Biocontrol and Institute of Entomology, School of Life Sciences, Zhongshan University, Guangzhou 510275, China 3 Department of Plant Protection, Nanjing Agriculture University, Nanjing 210095, China Department of Molecular and Cell Biology School of Life Sciences University of Science and Technology of China Hefei 230027China Department of Plant Protection Nanjing Agriculture University Nanjing 210095 China Department of Molecular and Cell Biology School of Life Sciences University of Science and Technology of China Hefei 230027China State Key Laboratory of Biocontrol and Institute of Entomology School of Life Sciences Zhongshan University Guangzhou 510275 China

Prothoracicotropic hormone (PTTH), a neuropeptide hormone stimulating the prothoracic glands to synthesize ecdysone, plays an important role in regulating postembryonic development in insects. The cDNA encoding PTTH was isolated and sequenced from the beet armyworm, Spodoptera exigua (Spe). The deduced a?mino acid sequence is composed of a signal peptide, a peptide (65 amino acids) of un-known function, and a mature PTTH molecule (111 amino acids). The Spe-PTTH shows similarities (45.5%―70.3%) to other known PTTHs reported in Lepidoptera species, but 7 cysteine r?esidues and the hydrophobic regions were conserved. Whole-mount immunocytochemistry by using an antiserum against recombinant Helicoverpa armigera PTTH showed that Spe-PTTH was synthesized in two pairs of neurosecretory cells in the S. exigua brain. Northern blot analysis demonstrates the presence of a 1.2-kb transcript in the brain. The Spe-PTTH mRNA is detectable at high levels at the wandering larval stage, early pupal stage, and pharate adult stage, suggesting that the Spe-PTTH gene might be corre-lated with molting, metamorphosis, and reproduction.

关键词： prothoracicotropic hormone molecular characterization Northern blot whole-mount immunocytochemistry Spodoptera exigua

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Interactions between a Root Knot Nematode (<i>Meloidogyne exigua</i>) and Arbuscular Mycorrhizae in Coffee Plant Development (<i>Coffea arabica</i>)

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American Journal of Plant Sciences 2013年第7期4卷 19-23页

作者： Raúl Alban Ricardo Guerrero Marcia Toro Laboratorio de Agroecosistemas Instituto de Zoología y Ecología Tropical Universidad Central de Venezuela Caracas Venezuela. Laboratorio de Biología de Vectores y Parásitos Universidad Central de Venezuela Caracas Venezuela Postgrado en Zoología Universidad Central de Venezuela Caracas Venezuela

This paper focuses on parasitic root knot nematodes (Meloidogyne exigua) and how to decrease their pathogenic effect on coffee plants (Coffea arabica), by examining the behaviour of and the interactions between nematodes, coffee plant and arbuscular mycorrhizae (AM). The experiment was carried out at the seedling stage, with six (6) treatments (plants with M. exigua, plants with arbuscular mycorrhizae, plants with both organisms, and the same time, first mycorrhizae plants, then nematodes were inoculated and vice versa). After 5 months the measured variables were: dry biomass (roots and shoot), nematode knots caused by M. exigua in root, nematode juvenile (J2) found in 100.0 g of soil, and mycorrhizal percentage. Plant nutrients (P and N) contents were analysed. Significant differences were found in all the variables, but concentration N content in plants. Plants with mycorrhizae and plants with mycorrhizae and then inoculated with nematodes have the same behaviour. Control plants and plants with nematode and then inoculated with mycorrhizae behave similarly. It is thought that arbuscular mycorrhizae are formed before the nematode infestation, allowing coffee plants to regain the energy lost by the parasitic interaction. AM may help coffee plants with lignifications of the plant cell wall cuticle. As the cuticle thickens it is more difficult for nematodes to penetrate and enter into plant roots. Therefore, arbuscular mycorrhizae help coffee plants to uptake and transport nutrients, improving its nutritional status and stabilizing nematode attacks. It is suggested that symbiotic interactions help neutralize parasitic interactions.

关键词： Arbuscular Mycorrhizae Meloidogyne exigua Coffee Plant Ecological Interactions Biotic Stress

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Temporal allocation of metabolic tolerance in the body of beet armyworm in response to three gossypol-cotton cultivars

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Science China(Life Sciences) 2009年第12期52卷 1140-1147页

作者： Marvin K HARRIS Department of Entomology Texas A&M University of USA

The nutrient composition and enzyme activities in larvae of the beet armyworm, Spodoptera exigua (Hbner), fed on high, medium or low gossypol cotton cultivars were examined at different time intervals. Significantly lower free fatty acid was observed in larvae fed for 6 h on high gossypol 'M9101' compared to larvae fed on the low (ZMS13) and intermediate (HZ401) gossypol cultivars. Significantly higher trypsin activity was observed in larvae fed on high gossypol 'M9101' for 24 h compared to those fed for 1, 4 and 6 h. Significantly higher catalase and total superoxide dismutase enzyme activities were observed in larvae of S. exigua fed on high gossypol 'M9101' compared with low gossypol cultivars 'ZMS13' and 'HZ401' for 1, 4, 6 and 24 h. However, significantly lower carboxylesterase and acetylcholinesterase enzyme activities were found in larvae fed on high gossypol 'M9101' compared with the other cultivars for 1, 4, 6 and 24 h. The interaction between cotton variety and beet armyworm infestation time significantly affected the carboxylesterase enzyme activity in S. exigua. The characterization of the effects of plant allelochemicals on herbivorous larvae is important for aiding understanding of plant-insect interaction as well as in devising solutions to pest problems by breeding plant resistance, identifying metabolic targets for insecticide development, etc.

关键词： Spodoptera exigua gossypol free fatty acid detoxification enzyme acetylcholinesterase total superoxide dismutase

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Effects of lead stress on Vg expression in the beet armyworm over five successive generations

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Journal of Integrative Agriculture 2019年第1期18卷 134-142页

作者： SU Hong-hua YANG Yong QIAN Yuan-yuan YE Zi-bo CHEN Yu-qing YANG Yi-zhong School of Horticulture and Plant Protection Yangzhou University

Heavy metals have been found to be endocrine disruptors in invertebrates.Lead is one of the most widespread elements of contamination,but there has been no research about the effects of lead stress on vitellogenin(Vg) gene expression in insects exposed to lead over multiple generations.In this paper,the effects of different concentrations of lead(0,0.3,4.8 and 76.8 mg kg^(–1)) on the expression of Vg in the beet armyworm over five successive generations were studied.The results showed that lead stress had significant effects on Vg expression in a dose-dependent manner.For females at the larval and adult stages,as lead concentration increased,Vg expression was significantly inhibited; for males at these two developmental stages,Vg expression was induced and increased as lead concentration increased.In addition,with the increase over stressed generations,inhibited effects for females and induced effects for males at the larval and adult stages became increasingly more obvious.However,at the pupal stage,Vg expression in the two genders was different from that at the larval and adult stages.The results indicate that lead stress can upregulate Vg expression in males which should be a useful indicator for environmental risk assessment.

关键词： Spodoptera exigua lead stress vitellogenin gene expression females males

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Field Trapping Technology of Sex Attractants against Major Pests of Broccoli

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Plant Diseases and Pests 2020年第1期11卷 22-29页

作者： Liu Weiming Wang Enguo Liu Yenan He Xianbiao Taizhou Vocational College of Science&Technology Taizhou 318020China Linhai Agricultural and Rural Bureau Linkai 317000China Taizhou Academy of Agricultural Sciences Linhai 317000China

[Objective]The paper was to explore the comprehensive sexual attraction control technology of main pests in broccoli,and to improve the green control technology of main pests in broccoli.[Method]The comprehensive control technology of sex attractants against major pests in broccoli was studied by using special sex attractants,lures and traps.[Result]The mixed quantities of Spodoptera litura,Spodoptera exigua and Plutella xylostella were fluctuated in a"W"shape in broccoli growing season in coastal area of Taizhou,central Zhejiang province,including seedling growth period(July to August),rosette and bud growth period(late September to early November),late harvest period of late-ripening broccoli(after early march).S.exigua and S.litura occurred severely at seedling stage;S.litura,S.exigua and P.xylostella occurred simultaneously at rosette and bud growth period;P.xylostella damaged florets of lateral branches in late harvest period.The trapping effects of combination A and E against S.litura,S.exigua and P.xylostella were the best;more than 1260 individuals of S.litura,S.exigua and P.xylostella were daily trapped per hectare,and the occurrence and damage of the three insect pests could be basically controlled.With the sex attract produced in Ningbo as an example,the combination of S.litura S type∶S.exigua S type∶P.xylostella blue type=1∶1∶4 distributed in five stars of clubs(space 10-20 cm)showed good comprehensive trapping effect in large area demonstration;traps were hung at the height of 0.5 m(0.2 m for P.xylostella),and lures were replaced once every 30-40 d.[Conclusion]Using special sex attractants,the comprehensive effects on S.litura,S.exigua and P.xylostella were determined at rosette and bud growth period of broccoli through combination modes,and supporting application technology were put forward,making great progress in exploring and improving sex attractant combination against insect pests.

关键词： Broccoli Sex attractant Spodoptera litura Spodoptera exigua Plutella xylostella

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吉林省大豆茎点霉叶斑病病原鉴定

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大豆科学 2019年第3期38卷 428-433,454页

作者：王梦奇白庆荣王大川杨丽娜吉林农业大学农学院吉林长春130118 吉林省农业技术推广总站吉林长春130033

从吉林省白山市靖宇县的大豆叶片上分离到一种真菌分离物,根据菌落形态特征、分生孢子形态特征、ITS、ACT、tub2、rpb2、TEF、LSU序列、致病性测试结果以及上述6种引物的系统发育树构建分析,将其鉴定为Boeremia exigua var.exigua,这是... 详细信息

从吉林省白山市靖宇县的大豆叶片上分离到一种真菌分离物,根据菌落形态特征、分生孢子形态特征、ITS、ACT、tub2、rpb2、TEF、LSU序列、致病性测试结果以及上述6种引物的系统发育树构建分析,将其鉴定为Boeremia exigua var.exigua,这是在我国首次发现该真菌感染大豆。系统发育树分析结果中,rpb2和TEF基因在种间关系上显示出了较好的区分度,更适用于Boeremia属的鉴定及研究种间关系。

关键词：大豆 Boeremia exigua var. exigua 鉴定

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白术枯斑病原鉴定、生物学特性及其对不同药剂的敏感性研究

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植物病理学报 2018年第5期48卷 682-692页

作者：张佳星李玲戴德江沈瑶张传清浙江农林大学农业与食品科学学院临安311300 浙江省农药检定管理总站杭州310020

白术(Atractylodes macrocephala Koidz.)是浙江省传统中药材"浙八味"之一。近年来在磐安县白术主产区发生一种新的枯斑病。为明确致病菌及其生物学特性和对杀菌剂的敏感性,本研究通过病原菌分离培养、柯赫氏法则验证、形态学特征观察,... 详细信息

白术(Atractylodes macrocephala Koidz.)是浙江省传统中药材"浙八味"之一。近年来在磐安县白术主产区发生一种新的枯斑病。为明确致病菌及其生物学特性和对杀菌剂的敏感性,本研究通过病原菌分离培养、柯赫氏法则验证、形态学特征观察,及ITS、LSU、rpb2和tub2多基因序列分析,发现引起该枯斑病的病原菌为Phoma exigua。生物学特性测定表明:该菌菌丝生长的最适温度为28℃,最适pH为8,最适碳源与氮源分别为可溶性淀粉和蛋白胨;分生孢子萌发最适温度24℃、相对湿度100%;菌丝和分生孢子的致死温度分别为47℃和54℃。不同药剂敏感性测定结果表明:咯菌腈对该菌菌丝生长的抑制作用最强,EC_(50)为0.24μg·mL^(-1);吡唑醚菌酯和嘧菌酯对其分生孢子萌发具有较强的抑制作用,EC_(50)分别为0.10和0.16μg·mL^(-1)。由P. exigua引起的白术枯斑病为国内外首次报道。

关键词：白术 Phoma exigua 多基因序列分析生物学特性敏感性测定

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甜菜夜蛾地理种群基因流与种群扩张历史分析

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湖北农业科学 2015年第14期54卷 3460-3465,3481页

作者：王兴亚周俐宏辽宁省农业科学院植物保护研究所沈阳110161 辽宁省农业科学院花卉研究所沈阳110161

利用mt DNA COI基因序列对采自我国7个地理种群的甜菜夜蛾(Spodoptera exigua Hübner)遗传多样性与种群扩张历史进行研究。结果表明,比对206条序列(序列长度为547 bp),共发现8个变异位点,定义5个单倍型,其中包括3个共享单倍型和2个独... 详细信息

利用mt DNA COI基因序列对采自我国7个地理种群的甜菜夜蛾(Spodoptera exigua Hübner)遗传多样性与种群扩张历史进行研究。结果表明,比对206条序列(序列长度为547 bp),共发现8个变异位点,定义5个单倍型,其中包括3个共享单倍型和2个独有单倍型;群体遗传多样性较低(Hd=0.320,π=0.000 70,Kxy=0.383)。群体间存在一定的遗传分化(FST=0.398)、基因流水平较低(Nm=0.378)。AMOVA分析表明,甜菜夜蛾遗传变异主要来自种群内,种群间变异水平较低。中性检验(Tajima’s D=-1.576,P<0.05;Fu and Li′s F=-3.834,P<0.05)与错配分布分析表明,我国北方甜菜夜蛾种群曾经历种群近期扩张。

关键词：甜菜夜蛾(Spodoptera exigua Hübner) COI基因遗传结构遗传分化

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甜菜夜蛾天敌的研究进展

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仲恺农业技术学院学报 2004年第2期17卷 68-73页

作者：陆小军董易之郑常格汤历广州市生物防治站广东广州510460 华南农业大学昆虫生态研究室广东广州510462

对甜菜夜蛾(Spodoptera exigua Hubner)的天敌种类,包括捕食性天敌、寄生蜂、寄生蝇、病原线虫、微孢子虫、病原真菌、细菌和病毒等及其应用进行了综述,并就当前应用中存在的问题及解决方法进行了探讨。

关键词：甜菜夜蛾(spodoptera exigua Hübner) 天敌生物防治

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