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Resistance risk assessment of six pyrethroids and acephate toward the resistant adult tarnished plant bug,Lygus lineolaris

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Insect Science 2024年第3期31卷 792-802页

作者： Yuzhe Du Shane Scheibener Yucheng Zhu Maribel Portilla Mingling Zhang USDA-ARS Southern Insect Management Research UnitStonevilleUSA USDA-ARS Pollinator Health in Southern Crop Ecosystem Research UnitStonevilleUSA

Due to rapidly developed resistance,pest management relies less on pyrethroids to control economically damaging infestations of the tarnished plant bug(TPB),Lygus lineolaris(Palisot de Beauvois)in cotton fields of Mississippi.Yet,pyrethroid resistance remains prevalent in TPB populations.This study assessed the resistance levels in adult TPB to six common pyrethroids and acephate.Resistant TBPs were collected from wild host plants in late October after harvest in the Mississippi Delta region of the United States.Based on LCso values,the field-resistant TPBs displayed higher resistance to permethrin,esfenvalerate,and bifenthrin(approximately 30 fold)and moderate resistance toα-cyhalothrin,β-cyfluthrin,5-cypermethrin,and acephate(approximately 15 fold).Further investigations showed that the inhibitors of three detoxification enzyme,triphenyl phosphate(TPP),diethyl maleate(DEM),and piperonyl butoxide(PBO)had synergistic effects on permethrin,γ-cyhalothrin,and bifenthrin in resistant TPBs.Furthermore,elevated esterase,GST,and P450 activities were significantly expressed in fieldresistant TPBs.Additionally,GST and esterase were reduced after 48 h exposure to certain pyrethroids at LCso dose.The synergistic and biochemical assays consistently indicated that P450 and esterase were involved in pyrethroid detoxification in TPBs.This study provides valuable information for the continued use of pyrethroids and acephate in controlling TPBs in cotton fields in the Mississippi Delta region of the United States.

关键词： cytochrome P450-monooxygenases(P450) detoxification enzyme activities esterase Lygus lineolaris pyrethroids and organophosphate resistance tarnished plant bug

Genome-wide identification, classification, and expression profiling of serine esterases and other esterase-related proteins in the tobacco hornworm, Manduca sexta

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Insect Science 2023年第2期30卷 338-350页

作者： Zelong Miao Chao Xiong Xiaolong Cao Tisheng Shan Qiao Jin Haobo Jiang Department of Entomology and Plant Pathology Oklahoma State UniversityOklahomaStillwaterUSA

Serine esterases(SEs)are hydrolases that catalyze the conversion of carboxylic esters into acids and alcohols.Lipases and carboxylesterases constitute two major groups of SEs.Although over a hundred of insect genomes are known,systematic identification and classification of SEs are rarely performed,likely due to large size and complex composition of the gene family in each species.Considering their key roles in lipid metabolism and other physiological processes,we have categorized 144 M.sexta SEs and SE homologs(SEHs),114 of which contain a motif of GXSXG.Multiple sequence alignment and phylogenetic tree analysis have revealed 39 neutral lipases(NLs),3 neutral lipase homologs(NLHs),11 acidic lipases(ALs),3 acidic lipase homologs(ALHs),a lipase-3,a triglyceride lipase,a monoglyceride lipase,a hormone-sensitive lipase,and a GDSL lipase.Eighty-three carboxylesterase genes encode 29α-esterases(AEs),12 AEHs(e.g.,SEH4-1–3),20 feruloyl esterases(FEs),2 FEHs,2β-esterases(BEs),2 integument esterases(IEs),1 IEH,4 juvenile hormone esterases,2 acetylcholinesterases,gliotactin,6 neuroligins,neurotactin,and an uncharacteristic esterase homolog.In addition to these GXSXG proteins,we have identified 26 phospholipases and 13 thioesterases.Expression profiling of these genes in specific tissues and stages has provided insights into their functions including digestion,detoxification,hormone processing,neurotransmission,reproduction,and developmental regulation.In summary,we have established a framework of information on SEs and related proteins in M.sexta to stimulate their research in the model species and comparative investigations in agricultural pests or disease vectors.

关键词： carboxylesterase esterase lipase phospholipase phylogenetic analysis RNA-Seq thioesterase

esterase-Activated Theranostic Prodrug for Dual Organelles-Targeted Imaging and Synergetic Chemo-Photodynamic Cancer Therapy

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CCS Chemistry 2022年第3期4卷 1028-1043页

作者： Jiabao Zhuang Nan Li Yaling Zhang Baolin Li Hanqi Wen Xinchan Zhang Tianyu Zhang Na Zhao Ben Zhong Tang Key Laboratory of Macromolecular Science of Shaanxi Province Key Laboratory of Applied Surface and Colloid Chemistry of Ministry of EducationSchool of Chemistry and Chemical EngineeringShaanxi Normal UniversityXi’an 710019 Department of Chemistry The Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and ReconstructionInstitute for Advanced StudyDepartment of Chemical and Biological EngineeringThe Hong Kong University of Science and TechnologyClear Water BayKowloonHong Kong 999077

Activatable prodrugs have received considerable attention in cancer therapy due to their high specificity and reduced side effects.However,the theranostic prodrug with multiple cancerous organelles targeting and combinational therapy is still rare.In this report,an esterase-responsive prodrug tetraphenylethylene functionalized quinolinium-ester-chlorambucil(TPEQC)was developed for dual organelles-targeted and image-guided cancer therapy through synergetic chemotherapy(CT)and photodynamic therapy(PDT).TPE-QC was constructed by conjugating an anticancer drug of chlorambucil with an aggregation-induced emission active photosensitizer of tetraphenylethylene functionalized hydroxyethyl quinolinium(TPE-QO)via the hydrolyzable ester linkage.The fluorescence and photosensitization of TPE-QC were initially quenched because of the photoinduced electron transfer(PET)effect.After reacting with esterase,the ester group of TPE-QC could be selectively hydrolyzed to release chlorambucil and TPE-QO,which terminated the PET process and switched on the fluorescence and photosensitization.Benefitting from the overexpressed esterase in cancer cells,TPE-QC could be efficiently activated in cancer cells rather than in normal cells,while the restoredfluorescence could preciselymonitor the release of TPE-QC.Importantly,activated TPE-QC accumulated in both organelles of lysosome and mitochondria,resulting in enhanced anticancer potency.In vivo experiments demonstrated that TPE-QC displayed efficient tumor microenvironment-activatable features and excellent tumor therapeutic effects through combinational CT and PDT.

关键词： aggregation-induced emission esterase lysosome mitochondria photodynamic therapy chemotherapy

Emerging significance of butyrylcholinesterase

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World Journal of Experimental Medicine 2024年第1期14卷 33-43页

作者： Gumpeny R Sridhar Lakshmi Gumpeny Department of Endocrinology and Diabetes Endocrine and Diabetes CentreVisakhapatnam 530002Andhra PradeshIndia Department of Internal Medicine Gayatri Vidya Parishad Institute of Healthcare and Medical TechnologyVisakhapatnam 530048Andhra PradeshIndia

Butyrylcholinesterase(BChE;EC 3.***.1.8),an enzyme structurally related to acetylcholinesterase,is widely distributed in the human body.It plays a role in the detoxification of chemicals such as succinylcholine,a muscle relaxant used in anesthetic practice.BChE is well-known due to variant forms of the enzyme with little or no hydrolytic activity which exist in some endogamous communities and result in prolonged apnea following the administration of succinylcholine.Its other functions include the ability to hydrolyze acetylcholine,the cholinergic neurotransmitter in the brain,when its primary hydrolytic enzyme,acetylcholinesterase,is absent.To assess its potential roles,BChE was studied in relation to insulin resistance,type 2 diabetes mellitus,cognition,hepatic disorders,cardiovascular and cerebrovascular diseases,and inflammatory conditions.Individuals who lack the enzyme activity of BChE are otherwise healthy,until they are given drugs hydrolyzed by this enzyme.Therefore,BChE is a candidate for the study of loss-of-function mutations in humans.Studying individuals with variant forms of BChE can provide insights into whether they are protected against metabolic diseases.The potential utility of the enzyme as a biomarker for Alzheimer’s disease and the response to its drug treatment can also be assessed.

关键词： esterase Acetylcholinesterase Variant Cholinergic Metabolic syndrome Cognition Knockout model

Hemagglutinin-esterase-fusion （HEF） protein of influenza C virus

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Protein & Cell 2016年第1期7卷 28-45页

作者： MingyangWang Michael Veilt Institute of Virology Veterinary Medicine Free University BerLin BerLin Germany

Influenza C virus, a member of the Orthomyxoviridae family, causes flu-like disease but typically only with mild symptoms. Humans are the main reservoir of the virus, but it also infects pigs and dogs. Very recently, influenza C-like viruses were isolated from pigs and cattle that differ from classical influenza C virus and might constitute a new influenza virus genus. Influenza C virus is unique since it contains only one spike protein, the hemagglu- tinin-esterase-fusion glycoprotein HEF that possesses receptor binding, receptor destroying and membrane fusion activities, thus combining the functions of Hemaggiutinin （HA） and Neuraminidase （NA） of influenza A and B viruses. Here we briefly review the epidemiology and pathology of the virus and the morphology of virus particles and their genome. The main focus is on the structure of the HEF protein as well as on its co- and post- translational modification, such as N-glycosylation, disulfide bond formation, S-acylation and proteolytic cleavage into HEF1 and HEF2 subunits. Finally, we describe the functions of HEF： receptor binding, esterase activity and membrane fusion.

关键词： influenza C virus HEF structure receptorbinding esterase membrane fusion

Identification and developmental expression of putative gene encoding juvenile hormone esterase(CpJHE-like) in codling moth, Cydia pomonella(L.)

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Journal of Integrative Agriculture 2019年第7期18卷 1624-1633页

作者： HUANG Cong WU Qiang JIANG Chun-yan XING Long-sheng SHI Guo-liang ZHANG Bin QIAN Wan-qiang LI You-zhi XI Yu YANG Nian-wan WAN Fang-hao College of Plant Protection Hunan Agricultural UniversityChangsha 410128P.R. China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R. China Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural SciencesShenzhen 518120P.R. China College of Plant Health and Medicine Qingdao Agricultural UniversityQingdao 266109P.R.China

Juvenile hormone esterase(JHE) is a key enzyme for insects,playing an important role in the regulation of insect growth,development,diapause and reproduction.We identified a complete putative JHE of Cydia pomonella(CpJHE-like) which is comprised of a 1 761 bp coding sequence(CDS) encoding 587 amino acid residues from the transcriptome data.The deduced protein sequence of CpJHE-like showed the highest identity of 60.44% with the Adoxophyes honmai JHE(AhJHE) and the minimal identity of 25.81% with Aedes aegypti JHE(AaJHE).CpJHE-like exhibited all the seven typical motifs of the functional JHEs and had the highly consistent tertiary structure with Manduca sexta JHE(MsJHE).Phylogenetic analysis showed that the CpJHE-like was close to two JHEs from the family Tortricidae.The CpJHE-like transcript level take a leap in the 3-day-old fifth instar larva,increased about 300-fold compared to the basal level.Tissue-specific expression profile showed that the CpJHE-like transcript was expressed mainly in the fat body.This study indicates that the CpJHE-like is the functional JHE,which may play vital roles in the development and reproduction of C.pomonella.

关键词： juvenile hormone esterase CpJHE-like codling moth Cydia pomonella

Theoretical studies of interaction models of human acetylcholine esterase with different inhibitors

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Science China Chemistry 2009年第11期52卷 1911-1916页

作者： ZHENG QingChuan CHU HuiYing NIU RuiJuan SUN ChiaChung State Key Laboratory of Theoretical and Computational Chemistry Institute of Theoretical ChemistryJilin UniversityChangchun 130023China

Alzheimer's disease(AD) is a progressive neurodegenerative disorder and one of the most common causes of dementia in the elderly.Acetylcholine esterase inhibitors(AChEI) are the main drugs used in the treatment of AD.In this work,docking studies have been performed in order to understand the interaction between a number of inhibitors(tacrine,rivastigmine,huperzine A,TV-3326(ladostigil),donepezil and anseculin) and acetylcholine esterase(AChE).The calculated binding affinities between inhibitors and AChE increase in the order tacrine

关键词： Alzheimer’s disease acetylcholine esterase inhibitor docking

Utilization of One Novel Microbial esterase WDEst9 in the Kinetic Resolution of (5)-Methyl 2-chloropropionate and (5)-Ethyl 2-chloropropionate

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Chemical Research in Chinese Universities 2019年第5期35卷 830-836页

作者： WANG Yilong XU Yongkai ZHANG Yun SUN Aijun HU Yunfeng CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301P.R.China Guangdong Key Laboratory of Marine Materia Medica South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301P.R.China Affiliated Hospital of Shandong University of Traditional Chinese Medicine Jinan 250014P.R.China

One novel microbial esterase WDEst9 from Dactylosporangium aurantiacum subsp. Hamdenensis NRRL 18085 was functionally characterized and the results demonstrated that the enantio-preference of WDEst9 was opposite to that of three other microbial esterases(BSE01701, PHE14 and Bae02030) in the kinetic resolution of racemic methyl lactate. We further investigated the potential of esterase WDEst9 in the kinetic resolution of both (±)-methyl 2-chloropropionate and (±)-ethyl 2-chioropropionate. The enantio-preference of WDEst9 was also interestingly opposite to that of esterases EST 12-7 and EstC10, and generated (S)-methyl 2-chloropropionate and (S)-ethyl 2-chloropropionate with high enantiomeric excess(both e.e.>98%) and high yield after many iterations of process optimization. Through genome mining, microbial esterase WDEst9 was characterized to be a novel esterase which may provide valuable complementary enantio-selectivity and possesses very good potential in the kinetic resolution of high value-added chiral chemicals.

关键词： Biocatalysis Novel esterase Asymmetric hydrolysis Complementary enantio-preference (5)-Methyl 2-chloropropionate (S)-Ethyl 2-chloropropionate

Artificial esterase Based on Self-assembly Gel Microspheres Constructed from Chitosan and Amino Acids

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Chemical Research in Chinese Universities 2019年第3期35卷 504-508页

作者： CAO Jing WANG Miao CHEN Weihua SHE Yongxin WANG Jing WANG Fengzhong LAO Shuibing Institute of Quality Standardization & Testing Technology for Agro-products Chinese Academy of Agricultural SciencesBeijing 100193P.R.China Key Laboratory ofAgrofood Safety and Quality (Beijing) Ministry ofAgricultureBeijing 100193P.R.China Institute of Agro-products Processing Science and Technology Chinese Academy of Agricultural SciencesBeijing 100193P.R.China Agro-products Quality Safety and Testing Technology Research Institute Guangxi Academy of Agricultural SciencesNanning 53003P.R.China

A novel artificial esterase based on chitosan and amino acids was synthesized in the present study. The Fmoc-His and Glu were linked to chitosan by active ester method(AEM). The hydroxide radical in chitosan, imidazole group of Fmoc-His and carboxyl from Glu formed a catalytic center of natural esterase. Gel microspheres were coated with a protective layer and a supporting layer by seiassembly construction function in carboxymethylcellulose sodium(CMCS) solution. As for catalytic activity, chitosan-His-Glu was found to be more efficient than chitosan- His and chitosan-Glu in mimicking the core catalytic sites of natural esterase, and the best ratio(mass ratio) of chitosan-His-Glu:CMCS was 1:3. Furthermore, metal ions, such as Ca^2+, Mg^2+, Fe^2+, etc., were able to improve the catalytic efficiency of artificial esterase. And tlie Lineweaver-Burk plot indicated that the catalytic kinetics of artificial esterase conformed to Michaelis-Menten equation.

关键词： Artificial enzyme esterase Cliitosan