检索结果-南通市图书馆

Development of 101 Novel est-Derived Single Nucleotide Polymorphism Markers for Zhikong Scallop (Chlamys farreri)

在线全文

学校读者我要写书评

暂无评论

Journal of Ocean University of China 2013年第3期12卷 403-412页

作者： LI Jiqin BAO Zhenmin LI Ling WANG Xiaojian WANG Shi HU Xiaoli Key Laboratory of Marine Genetics and Breeding (MGB) Chinese Ministry of EducationCollege of Marine Life SciencesOcean University of China

Zhikong scallop(Chlamys farreri) is an important maricultured species in *** researches on this species,such as population genetics and QTL fine-mapping,need a large number of molecular *** this study,based on the expressed sequence tags(est),a total of 300 putative single nucleotide polymorphisms(SNPs) were selected and validated using high resolution melting(HRM) technology with unlabeled *** them,101(33.7%) were found to be polymorphic in 48 individuals from 4 *** evaluation with 48 individuals from Qingdao population showed that all the polymorphic loci had two alleles with the minor allele frequency ranged from 0.046 to *** observed and expected heterozygosities ranged from 0.000 to 0.925 and from 0.089 to 0.505,*** loci deviated significantly from Hardy-Weinberg equilibrium and significant linkage disequilibrate was detected in one pair of *** gave significant hits for 72 of the 101 polymorphic SNP-containing *** four polymorphic SNP loci were predicted to be non-synonymous substitutions as they caused either the change of codons(33 SNPs) or pretermination of translation(1 SNP).The markers developed can be used for the population studies and genetic improvement on Zhikong scallop.

关键词： Zhikong scallop Chlamysfarreri SNP est HRM

Development, chromosome location and genetic mapping of est-SSR markers in wheat

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2005年第20期50卷 2328-2336页

作者： CHEN Haimei LI Linzhi WEI Xianyun LI Sishen LEI Tiandong HU Haizhou WANG Honggang ZHANG Xiansheng College of Agronomy Shandong Agricultural University/Shandong KeyLab of Crop Biology Tai'an 271018 China

A number of 151695 wheat expression sequence tags (ests) that originated from GenBank/dbest from July 14, 2003 to August 24, 2004 were used to search for simple sequence repeats (SSRs) with motif 2―5 bp, and 2038 simple sequence repeats (est-SSRs), which accounted for 1.34% of est database, were identified. Based on these SSR sequences, 249 est-SSR primer pairs and 166 amplified clear bands in various wheat cultivars were designed. These est-SSR markers can be used as new molecular markers in wheat and related species. Using Chinese Spring nulli-tetrasomic lines, 93 est-SSR primer pairs and 193 est-SSR loci were located on 19 wheat chromosomes except for 4A and 4B. Forty-three loci were mapped on 11 chromosomes of the genetic frame- work map previously constructed using recombinant inbred lines.

关键词：染色体遗传映射小麦序列重复 est

est-Based Identification of Genes Expressed in Skeletal Muscle of the Mandarin Fish (Siniperca chuatsi)

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genomics, Proteomics & Bioinformatics 2011年第1期9卷 30-36页

作者： Feng Ding Wuying Chu Peng Cui Meng Tao Ruixue Zhou Falan Zhao Songnian Hu Jianshe Zhang CAS Key Laboratory of Genome Sciences and Information Beijing Institute of Genomics Chinese Academy of Sciences Beijing 100029 China Department of Bioengineering and Environmental Science Changsha University Changsha 410003 China

To enrich the genomic information of the commercially important fish species, we obtained 5,063 high-quality expressed sequence tags （ests） from the muscle cDNA database of the mandarin fish （Siniperca chuatsi）. Clustering analysis yielded 1,625 unique sequences including 443 contigs （from 3,881 est sequences） and 1,182 sin- gletons. BLASTX searches showed that 959 unique sequences shared homology to proteins in the NCBI non-redundant database. A total of 740 unique sequences were functionally annotated using Gene Ontology. The 1,625 unique sequences were assigned to Kyoto Encyclopedia of Genes and Genomes reference pathways, and the results indicated that transcripts participating in nucleotide metabolism and amino acid metabolism are relatively abundant in S. chuatsi. Meanwhile, we identified 15 genes to be abundantly expressed in muscle of the mandarin fish. These genes are involved in muscle structural formation and regulation of muscle differentiation and development. The most remarkable gene in S. chuatsi is nuclease diphosphate kinase B, which is represented by 449 est sequences accounting for 8.86% of the total est sequences. Our work provides a transcript profile expressed in the white muscle of the mandarin fish, laying down a foundation in better understanding of fish genomics.

关键词： mandarin fish cDNA library est muscle

Technological paradigm and innovation of est of SMEs in China

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 1997年第1期9卷 120-126页

作者： Lu Yan Cai Ning School of Management,Zhejiang University,Hangzhou 310027,China School of Management Zhejiang University Hangzhou 310027 China

This article described the change of technological paradigm from environmentally un- friendly technology to environmentally sound technology(est),and reported results from the in- vestigation made in 1993-1995 of how China's small-medium enterprises(SMEs)have been inno- vating technology in response to environmental pressures and tried to identify factors affecting China' SMEs to innovate more environmentally sound products and processes.

关键词： est technology management SMEs.

Gene Identification and Expression Analysis of 86,136 Expressed Sequence Tags(est)from the Rice Genome

在线全文

学校读者我要写书评

暂无评论

Genomics, Proteomics & Bioinformatics 2003年第1期1卷 26-42页

作者： YanZhou JiabinTang MichaelG.Walker XiuqingZhang JunWang SongnianHu HuayongXu SigiLiu HuanmingYang JunYu JianWang YajunDeng JianhaiDong LinYe LiLin JunLi XuegangWang HaoHu YibinPan WeiLin WeiTian JingLiu LipingWei HangzhouGenomicsInstitute/InstituteofBioinformaticsofZhejiangUniversity/KeyLaboratoryofBioinformaticsofZhejiangProvince Hangzhou310007China BeijingGenomicsInstitute/CenterofGenomics&Bioinformatics ChineseAcademyofSciencesBeijing101300China StanfordUniversity StanfordCA94305USA CollegeofLifeSciences PekingUniversityBeijing100871China NexusGenomics MenloParkCA94025USA

Expressed Sequence Tag (est) analysis has pioneered genome-wide gene discovery and expression profiling. In order to establish a gene expression index in the rice cultivar indica, we sequenced and analyzed 86,136 ests from nine rice cDNA libraries from the super hybrid cultivar LYP9 and its parental cultivars. We assembled these ests into 13,232 contigs and leave 8,976 singletons. Overall, 7,497 sequences were found similar to the existing sequences in GenBank and 14,711 are novel. These sequences are classified by molecular function, biological process and pathways according to the Gene Ontology. We compared our sequenced ests with the publicly available 95,000 ests from japonica, and found little sequence variation, despite the large difference between genome sequences. We then assembled the combined 173,000 rice ests for further analysis. Using the pooled ests, we compared gene expression in metabolism pathway between rice and Arabidopsis according to KEGG. We further profiled gene expression patterns in different tissues, developmental stages, and in a conditional sterile mutant, after checking the libraries are comparable by means of sequence coverage. We also identified some possible library specific genes and a number of enzymes and transcription factors that contribute to rice development.

关键词： est expression profile

Identification and Expression Analysis of est-based Genes in the Bud of Lycoris longituba

在线全文

学校读者我要写书评

暂无评论

Genomics, Proteomics & Bioinformatics 2004年第1期2卷 43-46页

作者： YonglanCui XinyeZhang YanZhou HongYu LinTao LuZhang JianZhou QiangZhuge YoumingCai MinrenHuang KeyLaboratoryofTreeGeneticEngineeringofNanjingForestryUniversity Nanjing210037China HangzhouGenomicsInstitute／JamesD.WatsonInstituteofGenomeSciences ZhejiangUniversity／KeyLaboratoryofBioinformaticsofZhejiangProvinceHangzhou310008China DepartmentofForestry JiangxiAgricultureUniversityNanchang330055China ShanghaiMunicipalAgriculturalCommission Shanghai200003China

To obtain a primary overview of gene diversity and expression pattern inLycoris longituba, 4,992 ests (Expressed Sequence Tags) from L. longituba bud were se-quenced and4,687 cleaned ests were used for gene expression analysis. Clustered by the PHRAP program, 967contigs and 1,343 singlets were obtained. Blast search showed that 179 contigs and 227 singlets(totally 1,066 ests) had homologues in GenBank and 3,621 ests were novel.

关键词： lycoris longituba est

est-Based Identification of Genes Expressed in Skeletal Muscle of the Mandarin Fish (Siniperca chuatsi)

在线全文

学校读者我要写书评

暂无评论

Genomics,Proteomics & Bioinformatics 2011年第Z1期 30-36页

作者： Feng Ding1#, Wuying Chu2#, Peng Cui1, Meng Tao2, Ruixue Zhou2, Falan Zhao2, Songnian Hu1*, and Jianshe Zhang2* 1CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100029, China 2Department of Bioengineering and Environmental Science, Changsha University, Changsha 410003, China.

To enrich the genomic information of the commercially important fish species, we obtained 5,063 high-quality expressed sequence tags (ests) from the muscle cDNA database of the mandarin fish (Siniperca chuatsi). Clustering analysis yielded 1,625 unique sequences including 443 contigs (from 3,881 est sequences) and 1,182 sin- gletons. BLASTX searches showed that 959 unique sequences shared homology to proteins in the NCBI non-redundant database. A total of 740 unique sequences were functionally annotated using Gene Ontology. The 1,625 unique sequences were assigned to Kyoto Encyclopedia of Genes and Genomes reference pathways, and the results indicated that transcripts participating in nucleotide metabolism and amino acid metabolism are relatively abundant in S. chuatsi. Meanwhile, we identified 15 genes to be abundantly expressed in muscle of the mandarin fish. These genes are involved in muscle structural formation and regulation of muscle differentiation and development. The most remarkable gene in S. chuatsi is nuclease diphosphate kinase B, which is represented by 449 est sequences accounting for 8.86% of the total est sequences. Our work provides a transcript profile expressed in the white muscle of the mandarin fish, laying down a foundation in better understanding of fish genomics.

关键词： mandarin fish cDNA library est muscle

Analysis of SSRs Derived from an est Library of Half-Smooth Tongue Sole Cynoglossus semilaevis

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Ocean University of China 2008年第3期7卷 339-342页

作者： LIU Yunguo BAO Baolong CHEN Songlin SUN Xiuqin College of Fisheries Ocean Universitv of China Qingdao 266003 P. R. China Key Laboratory of Marine and estuarine Fisheries Ministry of Agriculture East China Sea Fisheries ResearchInstitute Shanghai 200090 P. R. China College of Aqua-Life Science and Technology Shanghai Ocean University Shanghai 200090 P. R. China Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao 266071 P. R. China The First Institute of Oceanography State Oceanic Administration Qingdao 266061 P. R. China

A complementary DNA(cDNA) library was constructed from half-smooth tongue sole spleen. A long-read expressed sequence tag(est) database was generated,containing 3100 cDNA clones,of which 220 clones were fully sequenced. A total of 1060 non-redundant simple sequence repeats(SSRs) were obtained from the cDNA library. An average of 5 kb sequence generates 1 SSR in the half-smooth tongue sole spleen cDNA library. The proportion of the SSR unit size was different in the cDNA library. The monomeric repeats(51.4%) are the most abundant class of SSR in the dataset. The dimeric,trimeric,tetrameric and hexameric re-peats are represented in decreasing proportions of 27.2%,16.0%,2.8% and 1.9%,respectively. The frequency of pentameric repeats was observed the least(only 0.7%) . Most of the monomeric and dimeric repeats are distributed in 3' and 5' un-translation region. If translation regions are considered merely,trimeric repeats are the highest,accounting for 57% of the total microsatellites.

关键词： half-smooth tongue sole Cynoglossus semilaevis est microsatellite

Development and Characterization of Microsatellite Markers for the Pacific Abalone (Haliotis discus) via est Database Mining

在线全文

学校读者我要写书评

暂无评论

Journal of Ocean University of China 2008年第2期7卷 219-222页

作者： ZHAN Aibin BAO Zhenmin WANG Mingling CHANG Dan YUAN Jian WANG Xiaolong HU Xiaoli LIANG Chengzhu HU Jingjie Laboratory of Marine Genetics and Breeding （MGB） College of Marine Life Sciences Ocean University of China Qingdao 266003 P. R. China Shangdong Entry-Exit Inspection and Quarantine Bureau Qingdao 266002 P R. China

The est database of the Pacific abalone (Haliotis discus) was mined for developing microsatellite markers. A total of 1476 est sequences were registered in GenBank when data mining was performed. Fifty sequences (approximately 3.4%) were found to contain one or more microsatellites. Based on the length and GC content of the flanking regions, cluster analysis and BLASTN, 13 microsatellite-containing ests were selected for PCR primer design. The results showed that 10 out of 13 primer pairs could amplify scorable PCR products and showed polymorphism. The number of alleles ranged from 2 to 13 and the values of Ho and He varied from 0.1222 to 0.8611 and 0.2449 to 0.9311, respectively. No significant linkage disequilibrium (LD) between any pairs of these loci was found, and 6 of 10 loci conformed to the Hardy-Weinberg equilibrium (HWE). These est-SSRs are therefore potential tools for studies of intraspecies variation and hybrid identification.

关键词： microsatellite Pacific abalone Haliotis discus est