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Human umbilical cord-derived mesenchymal stem cells differentiate into epidermal-like cells using a novel co-culture technique

Human umbilical cord-derived mesenchymal stem cells differen...

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第七届全国医学生物化学与分子生物学和第四届全国临床应用生物化学与分子生物学联合学术研讨会暨医学生化分会会员代表大会

作者： Li Dong-jie Chai Jia-ke Han Yan-fu Sun Tian-jun Burn Institute the First Affiliated Hospital of PLA General Hospital

Human umbilical cord-derived mesenchymal stem cells(hUCMSCs) isolated from human umbilical Wharton’s Jelly are a population of primitive and pluripotent *** different specific conditions,they can differentiate into different cells,such as adipocytes,osteoblasts,chondrocytes, neurocytes,endothelial cells ***,few reports on differentiating hUCMSCs into epidermal cells in vitro were *** are several methods of MSCs differentiation for choice,such as the specific induction medium,direct or indirect *** this study,We have developed a microporous membrane-based indirect co-culture system for hUCMSCs differentiation,in which epidermal stem cells were seeded on the bottom of microporous membrane and the culture flask of the 6-well dish,hUCMSCs were seeded on the top of microporous *** system allows real-time conditioning of the culture medium with human epidermal cells while still maintaining the complete separation of the two cell types. HUCMSCs in this indirect co-culture system expressed the early markers of epidermal cell lineage,P63, cytokeratinl9(CK19),andβ*** morphologies were changed from spindle-like fibroblastic appearances to oblate or irregular shapes under phase contrast *** these data not only highlight the possibility of in vitro control of hUCMSCs into epidermal lineage,but also suggest a novel indirect co-culture model for MSCs differentiation.

关键词： human umbilical cord-derived mesenchymal stem cells differentiation epidermal-like cells microporous membrane indirect co-culture system

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