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Aptamer-induced in-situ growth of acetylcholinesterase-Cu_(3)(PO_(4))_(2)hybrid nanoflowers for electrochemical detection of organophosphorus inhibitors

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Nano Research 2023年第10期16卷 12134-12143页

作者： Limin Yang Linjiao Qu Xiaolong Zhang Mingming Li Zhen Liu State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and Biotechnology China University of Petroleum(East China)Qingdao 266580China Institute of Biomedical Engineering Shenzhen Bay LaboratoryShenzhen 518055Chin

enzyme-inorganic hybrid nanoflowers(HNFs)have shown excellent sensing capabilities due to their large specific surface area as well as the simplicity and mildness of the preparation ***,coupling HNFs to electrodes to fabricate a uniform and controllable enzymatic electrochemical sensing interface remains a ***,we proposed an aptamer-induced insitu fabrication strategy for preparing an HNF-based electrochemical sensor with ideal *** to this strategy is the introduction of acetylcholinesterase(AChE)-specific binding aptamer(Apt),which induces the in-situ growth of AChE-copper phosphate(Cu_(3)(PO_(4))_(2))HNFs on the surface of carbon paper(CP).In addition,a dense gold nanoparticle(AuNP)layer was electrodeposited on the CP for anchoring Apt and further extending the electroactive surface *** prepared AChECu_(3)(PO_(4))_(2)HNF/Apt/AuNP/CP biosensor exhibited a wide detection range from 1 to 107 pM for the four organophosphorus inhibitors,including isocarbophos,dichlorvos,methamidophos,and parathion,with detection limits down to 0.016,0.028,0.071,and 0.113 pM,*** the reactivation of pralidoxime chloride,the electrode can still recover 98.1%of the response after five times of repeated *** real sample detection,the biosensor achieved high recoveries from 96.45%to 100.13%.The detection target may be extendable to other AChE inhibitors(e.g.,drugs for Alzheimer’s disease).This study demonstrates for the first time the feasibility of using aptamers as an inducer to fabricate an electrochemical enzyme sensing interface *** strategy can be used to fabricate other enzyme-based biosensors and therefore has broader applications.

关键词： in-situ fabrication aptamer enzyme-inorganic hybrid nanoflowers electrochemical biosensor acetylcholinesterase inhibitors

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