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Detection and Identification of enteroviruses RNA by Using Polymerase Chain Reaction

Journal of Huazhong University of Science and Technology(Medical Sciences) 1998年第3期18卷 156-160页

作者：陈宗波董永绥崔雯 Department of Pediatrics Tongji Hospital Tongji Medical University Wuhan

For rapid diagnosis of enteroviral infection in clinic practice, we developed a reverse transcription and polymerase chain reaction (RT-PCR) *** homologous to the conserved 5' non-coding region were designed by analyzing enteroviral genomes, and then they were used to enzymatically amplify RNA from 31 prototype enteroviral strains and enteroviruses (EV) in cerebrospinal fluid (CSF) of 34 cases of aseptic meningitis and 11 cases of aseptic encephalitis. The RT-PCR products generated with these enteroviral primers were analyzed by agar gel electrophoresis and dot blot hybridization analysis. 31 EV strains showed an obvious monoclonal amplification band, and all dot blot hybridization results were positive. Four other viruses and cells cultured were all negative. The study of sensitivity of the RT-PCR showed that amplification production were positive to 10(-2)- 10(-3) 50% tissue culture infective doses. With this assay, 21(61.8%) of 34 aseptic meningitis and 8 (72. 7%) of 11 aseptic encephalitis contained EV RNA in CSF samples. Two cases of meningitis and one of encephalitis with EV infection were still positive during convalescence. Our results suggest that this RT-PCR method was a fast, sensitive and specific technique for detection of common EV infection.

关键词： enteroviruses polymerase chain reaction

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Stability and infectivity of enteroviruses on dry surfaces:Potential for indirect transmission control

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Biosafety and Health 2023年第6期5卷 339-345页

作者： Pichamon Sittikul Pimolpachr Sriburin Jittraporn Rattanamahaphoom Warisa Nuprasert Nipa Thammasonthijarern Janjira Thaipadungpanit Weerawan Hattasingh Nathamon Kosoltanapiwat Pilaipan Puthavathana Supawat Chatchen Department of Tropical Pediatrics Faculty of Tropical MedicineMahidol UniversityBangkok 10400Thailand Department of Parasitology Faculty of Veterinary MedicineKasetsart UniversityBangkok 10900Thailand Department of Clinical Tropical Medicine Faculty of Tropical MedicineMahidol UniversityBangkok 10400Thailand Mahidol-Oxford Tropical Medicine Research Unit Faculty of Tropical Medicine Mahidol UniversityBangkok 10400Thailand Department of Microbiology and Immunology Faculty of Tropical MedicineMahidol UniversityBangkok 10400Thailand Center for Research and Innovation Faculty of Medical TechnologyMahidol UniversityNakhon Pathom 73170Thailand

Hand,foot,and mouth disease(HFMD)is a contagious disease mainly occurring in young children,and outbreaks commonly occur among young children in the Asia–Pacific region including ***,the World Health Organization(WHO)monitors HFMD in the Western Pacific region to detect outbreaks and other significant events by the Regional Event Based Surveillance *** is mainly caused by a group of enteroviruses(EVs)transmitted through direct contact(person to person)and indirect contact with contaminated objects(surface-to-hand).However,few studies have examined the surface stability of *** this study,we investigated the stability of enterovirus A71(EV-A71)and coxsackievirus A16(CVA16)on three different dry surfaces(wood,plastic,and stainless steel)using the endpoint titration using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide(MTT)staining of viable cells and real-time polymerase chain reaction(viral genome detection).The results revealed that virus infectivity dramatically decreased within a few hours on dry ***,viral RNA could be detected on dry surfaces for up to 28 *** heat inactivation,both EV-A71 and CVA16 were inactivated after exposure to 60°C for 15 *** on virus stability on different dry surfaces will provide useful information for HFMD transmission control.

关键词： enteroviruses EV-A71 Genomic RNA Hand foot and mouth disease MTT assay Stability

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Can Non-Polio enteroviruses Be Tamed with a Vaccine to Minimize Paralysis Caused by Them?

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World Journal of Vaccines 2015年第1期5卷 54-59页

作者： Omesh Kumar Bharti Corporation Health Officer Municipal Corporation Shimla India

Background: While we are inching towards global eradication of polio, the paralysis due to non-polio viruses (NPEV) poses greater challenge. Factors responsible for causing Acute Flaccid Paralysis (AFP) were studied in 3596 AFP patients in 64 districts of Uttar-Pradesh, India, to observe indirect relationship of AFP with wild polio as well as NPEV. A recent study suggests the need to investigate polio virus negative but NPEV positive AFP cases. Methods: The lab results of the stool samples of these children were line listed and analysed to observe the association of various factors with respect to presence of paralysis on 60 follow-up days. Taking zero OPV dose AFP cases as a biological base, we studied the relationship of presence of paralysis at 60 follow-up days to that of presence of NPEV in stool samples while polio virus was present or absent. Results: 70 of the 86 AFP cases (81%) with zero OPV dose and having only NPEV isolated in stool samples were having paralysis at 60 follow-up days. There were 4.54% (162) AFP cases, which did not carry any polio virus but were having NPEV isolated in the stool samples and paralysis at 60 follow-up days. 79% (75/95) of zero OPV dose children, who were having residual weakness at 60 follow-up days, were carrying both polio virus as well as NPEV in their stool samples. Total AFP cases, having residual weakness at 60 follow-up days and having NPEV in stool samples, decreased with increase in OPV doses;a behavior similar to what wild polio viruses (WPV) have to OPV. Conclusions: Maybe polio like NPEV is active for causing severe paralysis in children and is responding to the OPV. As is evident in the studies by M. Margalith, B. Fattal et al. [1] that there is an antibody response to the enteroviruses, we can think of coming out with a vaccine against the enteroviruses. Therefore, enterovirus vaccine can be produced on similar lines to that of OPV, as now we have enough isolates of NPEV. Effective NPEV surveillance system also

关键词： Polio Non-Polio Paralysis enteroviruses Vaccine

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EV713C protease cleaves host anti-viral factor OAS3 and enhances virus replication

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Virologica Sinica 2022年第3期37卷 418-426页

作者： Xiaolei Zhou Li Tian Jian Wang Baisong Zheng Wenyan Zhang Center for Infectious Diseases and Pathogen Biology Institute of Virology and AIDS ResearchKey Laboratory of Organ Regeneration and Transplantation of the Ministry of EducationThe First Hospital of Jilin UniversityJilin130021China

The global spread of enteroviruses(EVs)has become more frequent,severe and ***(IFN)I has been proved to control EVs by regulating IFN-stimulated genes(ISG)expression.20-50-oligoadenylate synthetases 3(OAS3)is an important ISG in the OAS/RNase L antiviral *** relationship between OAS3 and EVs is still ***,we reveal that OAS3,superior to OAS1 and OAS2,significantly inhibited EV71 replication in ***,EV71 utilized autologous 3C protease(3C^(pro))to cleave intracellular OAS3 and enhance viral ***,a human rhinovirus 3C protease inhibitor,completely abolished the cleavage of EV713C^(pro)on *** the proteolytically deficient mutants H40G,E71A,and C147G of EV713C^(pro)also lost the ability of OAS3 ***,the Q982-G983 motif in C-terminal of OAS3 was identified as a crucial 3C^(pro)cutting *** investigation indicated that OAS3 inhibited not only EV71 but also Coxsackievirus B3(CVB3),Coxsackievirus A16(CA16),Enterovirus D68(EVD68),and Coxsackievirus A6(CA6)***,unlike other four subtypes,CA163C^(pro)could not cleave *** key amino acids variation Ile36 and Val86 in CA163C^(pro)might result in weak and delayed virus replication of CA16 because of failure of OAS and 3AB *** works elucidate the broad anti-EVs function of OAS3,and illuminate a novel mechanism by which EV71 use 3C^(pro)to escape the antiviral effect of *** findings can be an important entry point for developing novel therapeutic strategies for multiple EVs infection.

关键词： enteroviruses EV71 3C protease Cleavage Anti-viral factor OAS3

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Global study of viral myocarditis: A systematic review and meta-analysis

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Journal of Acute Disease 2020年第1期9卷 1-13页

作者： Masoud Dadashi Taher Azimi Ebrahim Faghihloo Department of Microbiology School of MedicineAlborz University of Medical SciencesKarajIran Non Communicable Diseases Research Center Alborz University of Medical SciencesKarajIran Department of Pathobiology School of Public HealthTehran University of Medical SciencesTehranIran Students Scientific Research Center Tehran University of Medical SciencesTehranIran Department of Microbiology School of MedicineShahid Beheshti University of Medical SciencesTehranIran

Objective:To investigate the prevalence of viral myocarditis ***:We conducted a systematic search for the prevalence of the most common viruses in myocarditis and 75 studies were included for statistical analysis of the prevalence of adenovirus,hepatitis C virus,cytomegalovirus,Ebola virus,human herpesvirus 6,influenza virus,parvovirus,and non-polio ***:The highest prevalence was related to B19(25.0%)and non-polio enteroviruses(18%).The prevalence of human herpesvirus 6,cytomegalovirus,and Ebola virus was12.8%,5.5%,and 3.1%,*** C virus accounted for 6.1%of the disease,the adenoviruses contributed to 5.2%of viral *** lowest incidence was related to the influenza virus with 2.0%.Conclusions:Treatment of myocarditis is still problematic and may depend on the etiologic *** it is important to know the commonly occurring viral factors in myocarditis and timely diagnosis and treatment are also imperative.

关键词： Virus Myocarditis Meta-analysis B19 Non-polio enteroviruses

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