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Asian Pacific Journal of Tropical Medicine 2011年第5期4卷 391-396页

作者： Dinesh Kumar Patel Rajesh Kumar Damiki Laloo Siva Hemalatha Pharmacognosy Research Laboratory Department of PharmaceuticsInstitute of TechnologyBanaras Hindu UniversityVaranasi-221005.India

Objective:To investigate the naturally occurring antioxidant for the first time from the different solvent fractions of Hybanthus enneaspermus（***） Linn ***（Violaceae）. Methods:Different fractions of *** were tested for total phenolic content,and in vitro antioxidant activity was measured by total antioxidant assay,DPPH assay,reducing power, nitric oxide（NO）,hydrogen peroxide（H2O2） scavenging ***:The ethyl acetate（EA） fraction was found to have high levels of phenolic content[（212.15±0.79） mg GAE/g].The EA fraction exhibited higher total antioxidant capacity,higher percentage of DPPH radical scavenging activity[（127.07±2.29） Fg/mL],nitric oxide[（245.16±1.44）μg/mLJ,hydrogen peroxide[（227.38±7.18）μg/mL],deoxyribose[（270.61±8.72）μg/mL]and higher reducing *** was a significant correlation between total phenolic content and total antioxidant activity（r =0.972）. Conclusions:These results reveal that EA fraction of *** has strong antioxidant potential compared with other *** further study has been extended to the isolation of the possible compound that is responsible for having antioxidant property.

关键词： Hybanthus enneaspermus Antioxidant Total phenolics

Evaluation of in vitro aldose reductase inhibitory potential of different fraction of Hybanthus enneaspermus Linn ***

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Asian Pacific Journal of Tropical Biomedicine 2012年第2期2卷 134-139页

作者： Patel DK Kumar R Kumar M Sairarn K Hemalatha S Pharmacognosy Research Laboratory Department of PharmaceuticsInstitute of TechnologyBanaras Hindu University

Objective:To evaluate the aldose reductase inhibitory(ARI)activity of different fractions of Hybanthus enneaspermus for potential use in diabetic ***:Total phenol and flavonoid content of different fractions was *** activity of different fractions in rat lens was investigated in ***:The results showed significant level of phenolic and flavonoid content in ethyl acetate fraction[total phenol(212.15±0.79 mg/g),total flavonoid(39.11±2.27mg/g)]and aqueous fraction[total phenol(140.62±0.57mg/g),total flavonoid(26.07±1.49 mg/g)]as compared with the chloroform fraction[total phenol(68.56±0.51mg/g),total flavonoid(13.41±0.82mg/g)]and petrolium ether fraction[total phenol(36.68±0.43mg/g),total flavonoid(11.55±1.06mg/g)].There was a significant difference in the ARI activity of each fraction,and it was found to be the highest in ethyl acetate fraction[IC_(50)(49.26±1.76μg/mL)]followed by aqueous extract[IC_(50)(70.83±2.82μg/mL)]and it was least in the petroleum ether fraction[IC_(50)(118.89±0.71μg/mL)].Chloroform fraction showed moderate activity[IC_(50)(98.52±1.80μg/mL)].Conclusions:Different fractions showed significanct amount of ARI activity,where in ethyl acetate fraction it was found to be maximum which may be due to its high phenolic and flavonoid *** extract after further evaluation may be used in the treatment of diabetic cataract.

关键词： Hybanthus enneaspermus Total phenol Total flavonoid Rat lens Aldose reductase Diabetic cataract Different fractions

Antidiabetic and in vitro antioxidant potential of Hybanthus enneaspermus(Linn) *** in streptozotocin—induced diabetic rats

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Asian Pacific Journal of Tropical Biomedicine 2011年第4期1卷 316-322页

作者： Patel DK Kumar R Prasad SK Sairam K Hemalatha S Pharmacognosy Research Laboratory Department of PharmaceuticsInstitute of TechnologyBanaras Hindu University

Objective:To evaluate antidiabetic and antioxidant potential of Hybanthus enneaspermus in different ***:The oral glucose tolerance test(OGTT) and normoglycemic effect of alcoholic extract of Hybanthus enneaspermus(AHE) were evaluated at a dose of 125,250 and 500mg/kg *** hypoglycemic activity and effect on body weight were tested at 250 and 500 mg/kg *** day for 21 days in Streptozotocin(STZ) induced diabetic ***,glucose uptake by hemidiaphram was also *** total polyphenolic and flavonoid were determined and their correlation with various antioxidant assays was also ***:The results showed high level of phenolic content in *** also exhibited higher total antioxidant capacity,good reducing power and a significant scavenger of reactive oxygen species like DPPH radical,nitric oxide,hydrogen peroxide and *** there was a significant increase in the body weight and decrease in the blood glucose level on treatment with the *** increased glucose uptake on isolated rat hemi-diaphragm compared to control ***:AHE reduce blood glucose level in STZ-induced diabetic *** does not show significant effect in normoglycemic study but showes significant effect in *** has significant antioxidant activity,which may be attributed to high phenolic content.

关键词： Hybanthus enneaspermus Diabetic Antioxidant Total phenol Flavonoid Flavonol Glucose uptake

Improved Micropropagation in <i>Hybanthus enneaspermus</i>L. Muell

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American Journal of Plant Sciences 2013年第6期4卷 1169-1172页

作者： Gandhi Premkumar Neelakantan Arumugam Sankaralingam Muthuramkumar Govidaraju Varatharaju Kaniappan Rajarathinam Department of Biotechnology Government College Technology Coimbatore India. Department of Biotechnology Pondicherry University Pondicherry India Department of Botany Virudhunagar Hindu Nadar Senthikumara Nadar College (Autonomous) Virudhunagar India Department of Botany Virudhunagar Hindu Nadar Senthikumara Nadar College (Autonomous) Virudhunagar India

Multiple shoots were induced on the cut ends of the leaf of Hybanthus enneaspermus on MS medium fortified with different concentration of cytokinins (KI and BAP) and in combination of KI and BAP. To enhance the multiple shoots induction, different types of auxins were used. IAA induced a maximum of 52.3 shoots per explants in combination with KI + BAP. The regenerated shoots show two different morphological leaves. Shoots are 100% rooted on half-strength MS medium supplemented with 2.46 μM·l-1 IBA. Rooted plantlets were successfully transferred to garden after acclimatization and they appeared morphologically normal.

关键词： Hybanthus enneaspermus Multiple Shoots Regeneration Morphology