检索结果-南通市图书馆

中国植物学会第十六次全国会员代表大会暨85周年学术年会

作者： Hongbo Li Yingzhu Li Qiong Zhao Tingting Li Liwen Jiang Caiji Gao Guangdong Provincial Key Laboratory of Biotechnology for Plant Development School of Life SciencesSouth China Normal University School of Life Sciences The Chinese University of Hong Kong

The endosomal sorting complex required for transport（ESCRT） machinery is an ancient system that mainly controls multivesicular body（MVB） biogenesis and MVBmediated membrane protein *** the canonical functions of endosomal sorting complex required for transport（ESCRT） in endosomal sorting have been well documented in plants,it is not known whether plant ESCRT components perform nonendosomal ***,we show that FYVE domain protein required for endosomal sorting 1（FREE1）,our recently identified plant-specific ESCRT component essential for endosomal sorting and plant growth,plays additionally non-endosomal functions in the nucleus in transcriptionally regulating abscisic acid（ABA） signaling.A portion of FREE1 protein shuttles to the nucleus upon ABA treatment in a phosphorylation-dependent ***2 kinases interact with FREE1 and are responsible for its phosphorylated modifications,which occur in the serine residues of FREE1 *** shuttling to the nucleus,FREE1 can interact with basic leucine zipper transcription factors（TFs） ABI5 and ABF4 to suppress their transcriptional activity by affecting the binding of these TFs to the promoters of downstream ***,the free1-ctmut plants with partial deletion of the Cterminus or mutation of the phosphorylated serine sites,which circumvent the lethality of free1 loss-of-function mutant and show normal vacuolar sorting and plant development under regular growth conditions,displays defects in ABA-induced phosphorylation and nuclear import of FREE1,leading to increased activity of ABAresponsive TFs and enhanced response to ***,our study demonstrates the moonlighting properties of plant ESCRT subunit FREE1,which has evolved nonendosomal function in the nucleus besides its role in membrane trafficking in cytoplasm.

关键词： ABA signaling endomembrane trafficking ESCRT complex FREE1 protein Phosphorylation

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Crosstalk between endomembrane trafficking and Plant ABA Signaling

Crosstalk between Endomembrane Trafficking and Plant ABA Sig...

引用

2018全国植物生物学大会

作者： Hongbo Li Yingzhu Li Caiji Gao Guangdong Provincial Key Laboratory of Biotechnology for Plant Development School of Life SciencesSouth China Normal University (SCNU)

The endosomal sorting complex required for transport(ESCRT) machinery is an ancient system that mainly controls multivesicular body(MVB) biogenesis and MVB-mediated membrane protein ***,we identified a plant unique ESCRT component named as FYVE domain protein required for endosomal sorting 1(FREE1),which functions as an ESCRT-I component to regulate MVB biogenesis and vacuolar protein sorting in plant *** these endosomal sorting roles of FREE1 in the cytoplasm,we recently further identified an unconventional and intriguing function of nuclear localized FREE1 in transcriptional regulation of abscisic acid(ABA) signaling *** a combination of genetic,molecular and cellular approaches,we found that FREE1 could be actively phosphorylated by SnRK kinases upon exogenous ABA treatment,and shuttled from cytosol to nucleus to repress the activity of several ABA-responsive transcription factors.A special allele of free 1-weak mutant with disruption of the cytoplasmic-nuclear shuttling of FREE 1 protein displayed hypersensitive to ABA treatment and ABA-induced ***,using forward genetic screening,we have already obtained lots of suppressors of free1-ctmut(soft mutant),which are valuable materials for further characterization of the crosstalk between endomembrane trafficking and ABA signaling in *** findings provide a previously unidentified regulatory function of an ESCRT component in transcriptional regulation of plant hormone signaling.

关键词： ESCRT machinery endomembrane trafficking ABA signaling FREE1

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

The Basal Level Ethylene Response is Important to the Wall and endomembrane Structure in the Hypocotyl Cells of Etiolated Arabidopsis Seedlings

引用

Journal of Integrative Plant Biology 2012年第7期54卷 434-455页

作者： Chan Xu Xiaoyan Gao Xiaobin Sun Chi-Kuang Wen National KeyLaboratory of Plant Molecular Genetics and National Center for Plant Gene Research(Shanghai) Institute of Plant Physiology and EcologyShanghai Institutes for Biological SciencesChinese Academy of Sciences300 Fenglin RdShanghai 200032China Shanghai Applied Protein Technology ShanghaiChina

The sub-cellular events that occur during the ethylene-modulated cell elongation were characterized by examining the ultra-structure of etiolated Arabidopsis seedling hypocotyl cells. Preventing the basal level ethylene response facilitated cell elongation, and the cells exhibited wall loosening and separation phenotype. Nearby the wall separation sites were frequently associated with an increase in the cortical rough endoplasmic reticulum （rER） membranes, the presence of paramural bodies, and the circular Golgi formation. The cortical rER proliferation and circular Golgi phenotype were reverted by the protein biosynthesis inhibitor cycloheximide. The cortical rER membranes were longer when the ethylene response was prevented and shortened with elevated ethylene responses. Proteomic changes between wild type and the ethylene-insensitive mutant ethylene insensitive2 （ein2） seedling hypocotyls indicated that distinct subsets of proteins involving endomembrane trafficking, remodeling, and wall modifications were differentially expressed. FM4-64 staining supported the proteomic changes, which indicated reduced endocytosis activity with alleviation of the ethylene response. The basal level ethylene response has an important role in endomembrane trafficking, biological materials transport and maintenance of the endomembrane organization. It is possible that endomembrane alterations may partly associate with the wall modifications, though the biological significance of the alterations should be addressed in future studies.

关键词： ER proliferation cell walls circular Golgi endomembrane trafficking ethylene.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Trans-Golgi Network-An Intersection of trafficking Cell Wall Components

引用

Journal of Integrative Plant Biology 2012年第11期54卷 875-886页

作者： Natasha Worden Eunsook Park Georgia Drakakaki Department of Plant Sciences University of CaliforniaDavis 95616USA

The cell wall, a crucial cell compartment, is composed of a network of polysaccharides and proteins, providing structural support and protection from external stimuli.

关键词： Cell wall chemical genomics endomembrane trafficking trans-Golgi network vesicle proteomics.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Fast-Suppressor Screening for New Components in Protein trafficking,Organelle Biogenesis and Silencing Pathway in Arabidopsis thaliana Using DEX-Inducible FREE1-RNAi Plants

引用

Journal of Genetics and Genomics 2015年第6期42卷 319-330页

作者： Qiong Zhao Caiji Gao PoShing Lee Lin Liu Shaofang Li Tangjin Hu Jinbo Shen Shuying Pan Hao Ye Yunru Chen Wenhan Cao Yong Cui Peng Zeng Sheng Yu Yangbin Gao Liang Chen Beixin Mo Xin Liu Shi Xiao Yunde Zhao Silin Zhong Xuemei Chen Liwen Jiang Centre for Cell & Developmental Biology and State Key Laboratory of Agrobiotechnology School of Life SciencesThe Chinese University of Hong Kong Department of Botany and Plant Sciences Institute of Integrative Genome BiologyUniversity of California CUHK Shenzhen Research Institute The Chinese University of Hong Kong School of Life Sciences The Chinese University of Hong Kong Beijing Genomics Institute at Shenzhen Section of Cell and Developmental Biology University of California San Diego Shenzhen Key Laboratory of Microbial Genetic Engineering College of Life SciencesShenzhen University State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources School of Life SciencesSun Yat-sen University

Membrane trafficking is essential for plant growth and responses to external *** plant unique FYVE domain-containing protein FREE1 is a component of the ESCRT complex（endosomal sorting complex required for transport）.FREE1 plays multiple roles in regulating protein trafficking and organelle biogenesis including the formation of intraluminal vesicles of multivesicular body（MVB）,vacuolar protein transport and vacuole biogenesis,and autophagic ***1 knockout plants show defective MVB formation,abnormal vacuolar transport,fragmented vacuoles,accumulated autophagosomes,and seedling *** further uncover the underlying mechanisms of FREE1 function in plants,we performed a forward genetic screen for mutants that suppressed the seedling lethal phenotype of FREE1-RNAi transgenic *** obtained mutants are termed as suppressors of free1（sof）.To date,229 putative sof mutants have been *** detecting of FREE1 protein with M3 plants further identified 84 FREE1-related ***145 mutants showing no reduction of FREE1 protein were termed as RNAi-related *** next-generation sequencing（NGS）of bulked DNA from F2 mapping population of two RNAi-related sof mutants,FREE1-RNAi T-DNA inserted on chromosome 1 was identified and the causal mutation of putative sof mutant is being identified *** FREE1-and RNAi-related sof mutants will be useful tools and resources for illustrating the underlying mechanisms of FREE1 function in intracellular trafficking and organelle biogenesis,as well as for uncovering the new components involved in the regulation of silencing pathways in plants.

关键词： Suppressors FREE1 endomembrane trafficking Arabidopsis NGS

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：