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neural Regeneration Research 2012年第10期7卷 761-765页

作者： Weihui Huang Dawei Zang Yi Lu Ping Jiang Department of Neurology Tianjin First Center Hospital Tianjin Medical University Tianjin 300192 China

This study aimed to investigate the number of amino methyl isoxazole propionic acid （AMPA） receptors and production of endogenous neural stem cells in the SOD1 G93AG1H transgenic mouse model of amyotrophic lateral sclerosis, at postnatal day 60 following administration of basic fibroblast growth factor （FGF-2）. A radioligand binding assay and immunohistochemistry were used to estimate the number of AMPA receptors and endogenous neural stem cells respectively. Results showed that the number of AMPA receptors and endogenous neural stem cells in the brain stem and sensorimotor cortex were significantly increased, while motor function was significantly decreased at postnatal days 90 and 120. After administration of FGF-2 into mice, numbers of endogenous neural stem cells increased, while expression of AMPA receptors decreased, whilst motor functions were recovered. At postnatal day 120, the number of AMPA receptors was negatively correlated with the number of endogenous neural stem cells in model mice and FGF-2-treated mice. Our experimental findings indicate that FGF-2 can inhibit AMPA receptors and increase the number of endogenous neural stem cells, thus repairing neural injury in amyotrophic lateral sclerosis mice.

关键词： amino methyl isoxazole propionic acid receptor amyotrophic lateral sclerosis basic fibroblast growth factor endogenous neural stem cells

Effects of repetitive transcranial magnetic on the proliferation of endogenous neural stem cells of Parkinson’s disease model mice

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Effects of repetitive transcranial magnetic on the prolifera...

Identification of key genes involved in recovery from spinal cord injury in adult zebrafish

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Ping GU~(1,2,*),Ai-Ping ZHU~(1,*),Li-Na ZHANG~2,Yan-Yong WANG~(1,2),Ming-Wei WANG~(1,2) 1 Laboratory of Brain Aging and Cognitive Neuroscience,Hebei Province,Shijiazhuang 050031,China 2 Department of Neurology,1~(st)Hospital of Hebei Medical University,Shijiazhuang 050031,China

＜正＞Objective To observe the effects of repetitive transcranial magnetic stimulation on the proliferation of endogenous neural stem cells of Parkinson’s disease model mice and the mood change.Methods 72 male C57BL/6J mice were randomly divided into four experimental groups:normal saline group（NS）,PD model group（PD）,shamrTMS group（s-rTMS） and rTMS group（rTMS）.The mice received MPTP injection to establish acute PD model mice.The mice in NS group were injected the same volume saline.And twenty-four hours after the last injection of MPTP,the mice in rTMS group received 5 trains of 1 pulses/s for 25 s,at an intensity of 1 Tesla（T） daily for 1, 3,7 consecutive days.Sham control mice were exposed to the same noise during the stimulation.No any treatment was performed in the mice of NS and PD group.Evaluate the mood change by the elevated-plus maze testing before and after rTMS treatment.The expression of nestin in subventricular zone was observed by immunohistochemical technique.Results（1） There were no statistical significance about percentage of opening arm time（OT%） account for total time among groups and at the different time points in each group,but after intervening the mice about OT% shows the tendency of decrease.（2） Compared to NS group,the number of nestin-positive cells of PD Group was increased on between the 3rd day and 7th day.There was no statistical significance in the number of nestin-positive cells between PD group and s-rTMS group.Compared to s-rTMS group and PD group,the number of nestin-positive cells of rTMS group was evidently increased.（3） The proliferation of eNSCs was time dependent.eNSCs proliferate along certain way toward the outside migrates gradually,some cells were able to migrate to the cerebral cortex on the 7th day.Conclusion rTMS can promote the eNSCs proliferation and migration.The proliferation of eNSCs was time dependent.eNSCs begin to proliferate on the 1st day,proliferation of eNSCs is obvious on the 3rd day,proliferation of eNSCs achieves the peak on the 7th day.

关键词： endogenous neural stem cells subventricular zone repetitive transcranial magnetic stimulation Parkinson’s disease

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neural Regeneration Research 2022年第6期17卷 1334-1342页

作者： Wen-Yuan Shen Xuan-Hao Fu Jun Cai Wen-Chang Li Bao-You Fan Yi-Lin Pang Chen-Xi Zhao Muhtidir Abula Xiao-Hong Kong Xue Yao Shi-Qing Feng International Science and Technology Cooperation Base of Spinal Cord Injury Tianjin Key Laboratory of Spine and Spinal CordDepartment of OrthopedicsTianjin Medical University General HospitalTianjinChina Tianjin Medicine and Health Research Center Tianjin Institute of Medical&Pharmaceutical SciencesTianjinChina School of Medicine Nankai UniversityTianjinChina

Zebrafish are an effective vertebrate model to study the mechanisms underlying recovery after spinal cord injury.The subacute phase after spinal cord injury is critical to the recovery of neurological function,which involves tissue bridging and axon regeneration.In this study,we found that zebrafish spontaneously recovered 44%of their swimming ability within the subacute phase(2 weeks)after spinal cord injury.During this period,we identified 7762 differentially expressed genes in spinal cord tissue:2950 were up-regulated and 4812 were down-regulated.These differentially expressed genes were primarily concentrated in the biological processes of the respiratory chain,axon regeneration,and cell-component morphogenesis.The genes were also mostly involved in the regulation of metabolic pathways,the cell cycle,and gene-regulation pathways.We verified the gene expression of two differentially expressed genes,clasp2 up-regulation and h1m down-regulation,in zebrafish spinal cord tissue in vitro.Pathway enrichment analysis revealed that up-regulated clasp2 functions similarly to microtubule-associated protein,which is responsible for axon extension regulated by microtubules.Down-regulated h1m controls endogenous stem cell differentiation after spinal cord injury.This study provides new candidate genes,clasp2 and h1m,as potential therapeutic intervention targets for spinal cord injury repair by neuroregeneration.All experimental procedures and protocols were approved by the Animal Ethics Committee of Tianjin Institute of Medical&Pharmaceutical Sciences(approval No.IMPS-EAEP-Q-2019-02)on September 24,2019.

关键词： axon regeneration clasp2 endogenous neural stem cells h1m microtubule nanog neural regeneration neurogenesis spinal cord injury subacute phase

Effects of leukemia inhibitory factor and basic fibroblast growth factor on free radicals and endogenous stem cell proliferation in a mouse model of cerebral infarction

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neural Regeneration Research 2012年第19期7卷 1469-1474页

作者： Weihui Huang Yadan Li Yufeng Lin Xue Ye Dawei Zang Department of Neurology Tianjin First Center HospitalTianjin Medical UniversityTianjin 300192China

The present study established a mouse model of cerebral infarction by middle cerebral artery occlusion, and monitored the effect of 25 tJg/kg leukemia inhibitory factor and （or） basic fibroblast growth factor administration 2 hours after model establishment. Results showed that following administration, the number of endogenous neural stem cells in the infarct area significantly increased, malondialdehyde content in brain tissue homogenates significantly decreased, nitric oxide content, glutathione peroxidase and superoxide dismutase activity significantly elevated, and mouse motor function significantly improved as confirmed by the rotarod and bar grab tests. In particular, the effect of leukemia inhibitory factor in combination with basic fibroblast growth factor was the most significant. Results indicate that leukemia inhibitory factor and basic fibroblast growth factor can improve the microenvironment after cerebral infarction by altering free radical levels, improving the quantity of endogenous neural stem cells, and promoting neurological function of mice with cerebral infarction.

关键词： leukemia inhibitory factor basic fibroblast growth factor endogenous neural stem cells free radical malondialdehyde nitric oxide glutathione peroxidase superoxide dismutase neuroprotection

Effect of valproic acid on endogenous neural stem cell proliferation in a rat model of spinal cord injury

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neural Regeneration Research 2009年第7期4卷 513-517页

作者： Guoxin Nan Ming Li Weihong Liao Jiaqiang Qin Yujiang Cao Youqiong Lu Department of Orthopedics Children's Hospital Chongqing Medical University Chongqing 400014 China Research Institute of Surgery Daping Hospital Third Military Medical University of Chinese PLA National Key Laboratory of TraumaBurning and Combined Injury Chongqing 400042 China

BACKGROUND： Valproic acid has been reported to decrease apoptosis, promote neuronal differentiation of brain-derived neural stem cells, and inhibit glial differentiation of brain-derived neural stem cells. OBJECTIVE： To investigate the effects of valproic acid on proliferation of endogenous neural stem cells in a rat model of spinal cord injury. DESIGN, TIME AND SETTING： A randomized, controlled, neuropathological study was performed at Key Laboratory of Trauma, Buming, and Combined Injury, Research Institute of Surgery, Daping Hospital, the Third Military Medical University of Chinese PLA between November 2005 and February 2007. MATERIALS： A total of 45 adult, Wistar rats were randomly divided into sham surgery （n = 5）, injury （n = 20）, and valproic acid （n = 20） groups. Valproic acid was provided by Sigma, USA. METHODS： Injury was induced to the T10 segment in the injury and valproic acid groups using the metal weight-dropping method. The spinal cord was exposed without contusion in the sham surgery group. Rats in the valproic acid group were intraperitoneally injected with 150 mg/kg valproic acid every 12 hours （twice in total）.MAIN OUTCOME MEASURES： Nestin expression （5 mm from injured center） was detected using immunohistochemistry at 1,3 days, 1, 4, and 8 weeks post-injury. RESULTS： Low expression of nestin was observed in the cytoplasm, but rarely in the white matter of the spinal cord in the sham surgery group. In the injury group, nestin expression was observed in the ependyma and pia mater one day after injury, and expression reached a peak at 1 week （P 〈 0.05）. Expression was primarily observed in the ependymal cells, which expanded towards the white and gray matter of the spinal cord. Nestin expression rapidly decreased by 4 weeks post-injury, and had almost completely disappeared by 8 weeks. At 24 hours after spinal cord injury, there was no significant difference in nestin expression between the valproic acid and injury groups. At 1 week, there was a significant increase in the number of n

关键词： spinal cord injury nestin endogenous neural stem cells valproic acid rats

Effect of bFGF on endogenous NSCs in rat hippocampus with global cerebral ischemia-reperfusion

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Effect of bFGF on endogenous NSCs in rat hippocampus with gl...