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Gold nanoparticles cause size-dependent inhibition of embryonic development during murine pregnancy

Nano Research 2018年第6期11卷 3419-3433页

作者： Xiaowei Ma Xiaolong Yang Yufei Wang Juan Liu Shubin Jin Shuyi Li Xing-Jie Liang Chinese Academy of Sciences (CAS) Center for Excellence in Nanoscience CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety National Center for Nanoscience and Technology of China No. 11 First North Road Zhongguancun Beijing 100190 China University of Chinese Academy of Sciences Beijing 100049 China College of Biotechnology Tianjin University of Science & Technology Tianjin 300457 China Tissue Engineering Lab Beijing Institute of Transfusion Medicine Beijing 100850 China Beijing Municipallnstitute of Labour Protection No. 55 Taoranting Road Xicheng District Beijing 100054 China

Gold nanoparticles （Au NPs） have been widely utilized in biomedical appli- cations owing to their attractive features and biocompatibility, which greatly increase the risk of humans＂ being exposed to Au NPs, including pregnant women. In contrast to mature cells, embryos are more susceptible to outside disruptive stimuli. Nonetheless, a possible inhibitory effect of nanomaterials on embryonic development is usually ignored as long as the NPs do not have significant cytotoxic effects. According to our results, a minimal ＂nontoxic＂ concentration of Au NPs during early pregnancy can have lethal inhibitory effects on embryos in vivo and in vitro. We conducted important experiments on the influence of Au NPs on embryonic development and found that Au NPs can disturb embryonic development in a size- and concentration-dependent manner. Au NPs of 15 nm in diameter downregulated the expression pattern of distinct germ layer markers both at mRNA and protein levels; this action prevented differentiation of all three embryonic germ layers. Consequentl~ fetal resorption was observed. Our work reveals the impact of Au NPs on embryonic development and will provide an important guidance and serve as a reference for biomedical applications of Au NPs with minimal side effects.

关键词： gold nanoparticles embryonic development fetal resorption differentiation

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Possible Effect of 30K Proteins in embryonic development of Silkworm Bombyx mori

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Acta Biochimica et Biophysica Sinica 2005年第5期37卷 355-361页

作者： Bo-Xiong ZHONG~1 Jian-Ke LI~(1.2) Jian-Rong LIN~3 Jian-She LIANG~4 Song-Kun SU~1 Hai-Sheng XU~1 Hai-Yan YAN~1 Ping-Bo ZHANG~5 Hiroshi FUJII~5 1 Laboratory of Molecular Biology of Silkworm and Honeybee, Department of Bioresource Science, College of Animal Science,Zhejiang University, Hangzhau 310029, China 2 Institute of Apicultural Research, Chinese Academy of Agricultural Science, Beijing 100093, China 3 Department of Sericulture, South China Agricultural University.Guangzhou 510642, China 4 Biochemistry Co. Ltd. of Zhejiang University, Hangzhou 310029. China 5 Faculty of Agriculture,Kyushu University, Fukuoka 812-81. Japan Laboratory of Molecular Biology of Silkworm and Honeybee Department of Bioresource Science College of Animal Science Zhejiang UniversityHangzhou 310029 China Institute of Agicultural Research Chinese Academy of Agricultural ScienceBeijing 100093 China Department of Sericulture South China Agricultural UniversityGuangzhou 510642 China Biochemistry Co. Ltd. of Zhejiang UniversityHangzhou 310029 China Faculty of Agriculture Kyushu UniversityFukuoka 812-81 Japan

The silkworm Bombyx mori possesses a 30K protein family of 3×10～4 Da,the biologicalfunctions of which have not been fully *** relationship between the 30K protein family and theembryonic development of temperature sensitive sex-linked mutant strain of silkworm was investigated bytwo dimensional polyacrylamide gel electrophoresis(2D-PAGE)and Matrix assisted laser desorption ionization-time of flight mass spectrometry(MALDI-TOF MS).The results show that protein spots 1-5 of the 30Kprotein family,mainly existing in normal strain,are possibly related to embryonic *** earlyconsumption of a 30K protein named 6G1-30K-1 and the accumulation of 30K proteins named 6G1-30K-3and 6G1-30K-4 are likely caused by the destruction of physiological balance in normal embryonic development,which may lead to lower hatchability of the temperature sensitive *** results suggest that reasonablemetabolism of 30K proteins is a prerequisite for the embryo's normal development.

关键词： silkworm Bombyx mori 30K protein embryonic development 2D-PAGE MALDI-TOF MS

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embryonic development of Pelteobagrus fulvidraco(Richardson,1846)

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Chinese Journal of Oceanology and Limnology 2006年第4期24卷 378-383页

作者：王卫民 Khalid ABBAS 严安生 Fisheries College Key Lab of Agricultural Animal Genetics Breeding and Reproduction of Ministry of Education Huazhong Agriculture University

For production enhancement and procedure upgrade, the developmental phases of labora-tory-reared eggs of catfish Pelteobagrus fulvidraco were investigated. Twenty mature females and 10 males were collected from Dadongmen wholesale fisheries market in Wuhan City on May 8, 2003. Zy-gotes were stripped from mature fish after hormone-induced ovulation, fertilized, and incubated through whole embryonic development. The fertilized eggs were stocked in density of 100 eggs/L in white square tanks of 10 L. Incubation water was dechlorinated tap water with continuous aeration. The tanks were lit directly with 60 W fluorescent bulbs with a 12 light:12 dark photoperiod. Water temperature, dissolved oxygen and pH were 29.0±0.5℃, 6.7±0.4 mg/L and 7.4±0.2, respectively. The results showed that the eggs of P. fulvidraco were yellow, sticky and contained much yolk. The mean diameter of fertilized eggs was 2.03 mm. At the water temperature of 29.0±0.5℃, the ontogenesis spent about 33 h after fertilization. From fertilization to hatching, the embryonic development can be divided into 30–40 phases, which var-ies in the emphasis and direction of development. The detailed embryonic movement was also described.

关键词： Pelteobagrusfulvidraco embryonic development embryonic movement

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IDH2 and GLUD1 depletion arrests embryonic development through an H4K20me3 epigenetic barrier in porcine parthenogenetic embryos

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Zoological Research 2024年第6期45卷 1175-1187页

作者： Cheng-Lin Zhan Qin-Yue Lu Song-Hee Lee Xiao-Han Li Ji-Dam Kim Gyu-Hyun Lee Jae-Min Sim Hyeon-Ji Song Ying-Yan Jin Xiang-Shun Cui Department of Animal Science Chungbuk National UniversityCheongjuChungbuk 28644Republic of Korea

Isocitrate dehydrogenase 2(IDH2)and glutamate dehydrogenase 1(GLUD1)are key enzymes involved in the production ofα-ketoglutarate(α-KG),a metabolite central to the tricarboxylic acid cycle and glutamine *** this study,we investigated the impact of IDH2 and GLUD1 on early porcine embryonic development following IDH2 and GLUD1 knockdown(KD)via doublestranded RNA(dsRNA)*** showed that KD reducedα-KG levels,leading to delayed embryonic development,decreased blastocyst formation,increased apoptosis,reduced blastomere proliferation,and ***,IDH2 and GLUD1 KD induced abnormally high levels of trimethylation of lysine 20 of histone H4(H4K20me3)at the 4-cell stage,likely resulting in transcriptional repression of embryonic genome activation(EGA)-related ***,KD of lysine methyltransferase 5C(KMT5C)and supplementation with exogenousα-KG reduced H4K20me3 expression and partially rescued these defects,suggesting a critical role of IDH2 and GLUD1 in the epigenetic regulation and proper development of porcine ***,this study highlights the significance of IDH2 and GLUD1 in maintaining normal embryonic development through their influence onα-KG production and subsequent epigenetic modifications.

关键词： A-ketoglutarate H4K20me3 IDH2 GLUD1 embryonic development

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Catalytic activity of Setd2 is essential for embryonic development in mice: establishment of a mouse model harboring patient-derived Setd2 mutation

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Frontiers of Medicine 2024年第5期18卷 831-849页

作者： Shubei Chen Dianjia Liu Bingyi Chen Zijuan Li Binhe Chang Chunhui Xu Ningzhe Li Changzhou Feng Xibo Hu Weiying Wang Yuanliang Zhang Yinyin Xie Qiuhua Huang Yingcai Wang Stephen DNimer Saijuan Chen Zhu Chen Lan Wang Xiaojian Sun Shanghai Institute of Hematology State Key Laboratory of Omics and DiseasesNational Research Center for Translational Medicine at ShanghaiRuijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicineand School of Life Sciences&BiotechnologyShanghai Jiao Tong UniversityShanghai 200025China CAS Key Laboratory of Tissue Microenvironment and Tumor Shanghai Institute of Nutrition and HealthShanghai Institutes for Biological SciencesUniversity of Chinese Academy of SciencesChinese Academy of SciencesShanghai 200031China Department of Clinical Laboratory The First People's Hospital of LianyungangThe Affiliated Lianyungang Hospital of Xuzhou Medical UniversityLianyungang 222000China Sylvester Comprehensive Cancer Center and Department of Medicine University of Miami Miller School of MedicineMiamiFL 33136USA Department of Biomedical Science Schmidt College of MedicineFlorida Atlantic UniversityBoca RatonFL 33431USA

SETD2 is the only enzyme responsible for transcription-coupled histone H3 lysine 36 trimethylation(H3K36me3).Mutations in SETD2 cause human diseases including cancer and developmental *** mice,Setd2 is essential for embryonic vascular *** that many epigenetic modifiers have recently been found to possess noncatalytic functions,it is unknown whether the major function(s)of Setd2 is dependent on its catalytic activity or ***,we established a site-specific knockin mouse model harboring a cancer patientderived catalytically dead Setd2(Setd2-CD).We found that the essentiality of Setd2 in mouse development is dependent on its methyltransferase activity,as the Setd2 CD/CD and Setd2^(−/−)mice showed similar embryonic lethal phenotypes and largely comparable gene expression ***,compared with Setd2^(−/−),the Setd2 CD/CD mice showed less severe defects in allantois development,and single-cell RNA-seq analysis revealed differentially regulated allantois-specific 5′Hoxa cluster genes in these two ***,this study clarifies the importance of Setd2 catalytic activity in mouse development and provides a new model for comparative study of previously unrecognized Setd2 functions.

关键词： Setd2 H3K36 methylation epigenetics embryonic development cancer

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Changes in embryonic development,juvenile growth and physiological adaptation of the cuttlefish Sepia pharaonis in response to photoperiod manipulation

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Journal of Oceanology and Limnology 2022年第5期40卷 2012-2027页

作者： Maowang JIANG Huan CHEN Shuangnan ZHOU Qingxi HAN Ruibing PENG Xiamin JIANG Key Laboratory of Applied Marine Biotechnology School of Marine SciencesNingbo UniversityNingbo 315211China

Photoperiod is a key environmental indicator for re gulating embryonic development,individual growth and physiological processes in aquatic *** this study,differences in embryonic development and performance of newly hatched cuttlefish juvenile exposed to five different cycles of light:dark(L:D):constant light,18 L:6D,12 L:12D,6 L:18D cycles,and constant darkness were *** exposure to light induced an accelerated rate of embryonic development,particularly after the red-bead *** component analysis(PCA)revealed that red-bead stage,heartbeat,endoskeleton formation,pigment appear,and six increments of cuttlebone were the main factors contributing to the embryonic *** that the duration time of these five stages were significantly different when exposed to photoperiod regimes,which may determine the duration of the incubation period of the *** term light has also affected the incubation parameters with an increased rate in hatching and shortened the incubation and hatching periods in the 12-24-h day length ***,constant light and darkness environment appeared to have a greater effect on the stress of embryonic development,mainly reflected in the yolk shed ratio and the inking rate in the egg ***,the increase in the day length has contributed to improve the growth and survival of juveniles in the 12-18-h day length range;however,juveniles exposed to constant light and darkness experienced worse re sults in terms of growth,tissue glycogen content,dige stive enzymes of the digestive glands,and metabolic enzymes of the *** finding suggest that prolonged light exposure accelerates the process of embryonic development,maximum feeding time is not necessarily a condition of optimal growth,and inappropriate light cycles can disturb the body’s endogenous ***,the optimal photoperiod for the embryos development and juvenile growth of Sepia pharaonis were 12 h and 12-18 h of day l

关键词： photoperiod embryonic development growth physiological changes cuttlefish

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DNA methylation-mediated expression of zinc finger protein 615 affects embryonic development in Bombyx mori

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Zoological Research 2022年第4期43卷 552-565页

作者： Guan-Feng Xu Cheng-Cheng Gong Yu-Lin Tian Tong-Yu Fu Yi-Guang Lin Hao Lyu Yu-Ling Peng Chun-Mei Tong Qi-Li Feng Qi-Sheng Song Si-Chun Zheng Guangdong Provincial Key Laboratory of Insect developmental Biology and Applied Technology Guangzhou Key Laboratory of Insect Development Regulation and Applied ResearchInstitute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouGuangdong 510631China Division of Plant Science and Technology College of AgricultureFood and Natural ResourcesUniversity of MissouriColumbia MO 65211USA

Cell division and differentiation after egg fertilization are critical steps in the development of embryos from single cells to multicellular individuals and are regulated by DNA methylation via its effects on gene ***,the mechanisms by which DNA methylation regulates these processes in insects remain ***,we studied the impacts of DNA methylation on early embryonic development in Bombyx *** methylation and transcriptome analysis of early embryos showed that DNA methylation events mainly occurred in the 5'region of protein metabolism-related *** transcription factor gene zinc finger protein 615(ZnF615)was methylated by DNA methyltransferase 1(Dnmt1)to be up-regulated and bind to protein metabolism-related ***1 RNA interference(RNAi)revealed that DNA methylation mainly regulated the expression of nonmethylated nutrient metabolism-related genes through *** same sites in the ZnF615 gene were methylated in ovaries and *** of ZnF615 using CRISPR/Cas9 gene editing decreased the hatching rate and egg number to levels similar to that of Dnmt1 *** of the ZnF615 methylation rate revealed that the DNA methylation pattern in the parent ovary was maintained and doubled in the offspring ***,Dnmt1-mediated intragenic DNA methylation of the transcription factor ZnF615 enhances its expression to ensure ovarian and embryonic development.

关键词： DNA methylation embryonic development Transcriptional regulation Epigenetic

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Changes during late-stage embryonic development from egg-juvenile to free-living hatchling in Chinese freshwater crab Sinopotamon yangtsekiense (Decapoda, Brachyura, Potamidae)

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Chinese Journal of Oceanology and Limnology 2013年第3期31卷 542-551页

作者：薛俊增刘艳 Neil CUMBERLIDGE 吴惠仙 Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Shanghai Ocean University Department of Biology Northern Michigan UniversityMarquette

This study expands on recent reports that direct development in the Chinese potamid freshwater crab Sinopotamon yangtsekiense involves the completion of all brachyuran larval stages (nauplius, zoea, and megalopa) inside the egg case during embryonic development. Detailed studies of embryonic development in this species revealed the presence of an additional larval stage (the egg-juvenile) between the megalopa and the free-living hatchling crab. We described and compared the appendages of the head, thorax, and abdomen of the egg-juvenile with those of the hatchling crab in S. yangtsekiense. Significant differences were found between most of the appendages of these two stages with a soft exoskeleton in the egg-juvenile, no joint articulation, a slimmer appearance, and a lack of setae when compared with the newly emerged free-living hatchling crab. These modifications of the appendages are related to the confinement within the egg case of the egg-megalopa and egg-juvenile during direct development, and the need for the free-living hatchling freshwater crab to move, feed, and respire. In marine crabs, the megalopa gives rise to the first crab stage whereas in freshwater crabs the egg-juvenile follows the megalopa and immediately precedes the free-living first crab stage.

关键词： Sinopotamon yangtsekiense freshwater crab embryonic development egg-juvenile hatchlingcrab appendage

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A CRISPR/RfxCas13d-mediated strategy for efficient RNA knockdown in mouse embryonic development

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Science China(Life Sciences) 2024年第11期67卷 2297-2306页

作者： Lin Zhang Shi-Meng Cao Hao Wu Meng Yan Jinsong Li Ling-Ling Chen Key Laboratory of RNA Innovation Science and EngineeringCAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyUniversity of Chinese Academy of SciencesChinese Academy of SciencesShanghai 200031China State Key Laboratory of Cell Biology Shanghai Key Laboratory of Molecular AndrologyShanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai 200031China Key Laboratory of Systems Health Science of Zhejiang Province School of Life ScienceHangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou 310024China School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China New Cornerstone Science Laboratory Shenzhen 518054China

The growing variety of RNA classes,such as mRNAs,lncRNAs,and circRNAs,plays pivotal roles in both developmental processes and various pathophysiological ***,our comprehension of RNA functions in live organisms remains limited due to the absence of durable and effective strategies for directly influencing RNA *** this study,we combined the CRISPR-RfxCas13d system with spermlike stem cell-mediated semi-cloning techniques,which enabled the suppressed expression of different RNA *** approach was employed to interfere with the expression of three types of RNA molecules:Sfmbt2 mRNA,Fendrr lncRNA,and circMan1a2(2,3,4,5,6).The results confirmed the critical roles of these RNAs in embryonic development,as their loss led to observable phenotypes,including embryonic lethality,delayed embryonic development,and embryo *** summary,our methodology offers a potent toolkit for silencing specific RNA targets in living organisms without introducing genetic alterations.

关键词： sperm-like stem cell semi-cloned technology CRISPR-RfxCas13d RNA knockdown embryonic development

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The structure of TRAF7 coiled-coil trimer provides insight into its function in zebrafish embryonic development

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Journal of Molecular Cell Biology 2024年第1期16卷 43-53页

作者： Xiaozhen Song Ruixing Hu Yi Chen Man Xiao Hong Zhang Shengnan Wu Qing Lu Molecular Diagnostic Laboratory Shanghai Children’s HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200040China Bio-X Institutes Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders(Ministry of Education)Shanghai Jiao Tong UniversityShanghai 200030China Laboratory of development and Diseases and State Key Laboratory for Medical Genomics Shanghai Institute of HematologyRuĳin HospitalShanghai JiaoTong University School of MedicineShanghai 200025China

TRAF7 serves as a crucial intracellular adaptor and E3 ubiquitin ligase involved in signal transduction pathways,contributing to immune responses,tumor progression,and embryonic *** mutations within the coiled-coil(CC)domain and WD40 repeat domain of TRAF7 could cause brain tumors,while germline pathogenic mutations contribute to severe developmental ***,the precise molecular mechanism underlying TRAF7 involvement in embryonic development remains *** this study,we employed zebrafish as an in vivo model ***7 knock down caused defects in zebrafish embryonic *** determined the crystal structure of TRAF7 CC domain at 3.3Åresolution and found that the CC region trimerization was essential for TRAF7 functionality during zebrafish embryonic ***,disease-causing mutations in TRAF7 CC region could impair the trimer formation,consequently impacting early embryonic development of ***,our study sheds light on the molecular mechanism of TRAF7 CC trimer formation and its pivotal role in embryonic development.

关键词： TRAF7 coiled-coil domain crystal structure trimerization embryonic development

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