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Road to full bioconversion of biowaste to biochemicals centering on chain elongation: A mini review

Journal of Environmental Sciences 2019年第12期31卷 50-64页

作者： Wenhao Han Pinjing He Liming Shao Fan Lü State Key Laboratory of Pollution Control and Resource Reuse Tongji UniversityShanghai 200092China Shanghai Institute of Pollution Control and Ecological Security Shanghai 200092China Institute of Waste Treatment and Reclamation Tongji UniversityShanghai 200092China Centre for the Technology Research and Training on Household Waste in Small Toums&Rural Area Ministry of Housing and Urban-Rural Development of China(MOHURD)China

Production of biochemicals from waste streams has been attracting increasing worldwide interest to achieve climate protection *** elongation(CE)for production of mediumchain carboxylic acids(MCCAs,especially caproate,enanthate and caprylate)from diverse biowaste has emerged as a potential economic and environmental technology for a sustainable *** present mini review summarizes the research utilizing various synthetic or real waste-derived substrates available for MCCA ***,the microbial characteristics of the CE process are surveyed and *** that a large proportion of recalcitrantly biodegradable biowaste and residues cannot be further utilized by CE systems and remain to be treated and disposed,we propose here a loop concept of bioconversion of biowaste to MCCAs making full use of the biowaste with zero *** could make possible an alternative technology for synthesis of value-added products from a wide range of biowaste,or even non-biodegradable waste(such as,plastics and rubbers).Meanwhile,the remaining scientific questions,unsolved problems,application potential and possible developments for this technology are discussed.

关键词： Organic waste Waste valorization Chain elongation Medium-chain carboxylic acids Functional microbes electron donors

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Biodegradation of Pentachloronitrobenzene by Labrys portucalensis pcnb-21 Isolated from Polluted Soil

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Pedosphere 2011年第1期21卷 31-36页

作者： LI Rong ZHENG Jin-Wei NI Bin CHEN Kai YANG Xiu-Juan LI Shun-Peng JIANG Jian-Dong Key Laboratory of Microbiological Engineering of Agricultural Environment Ministry of Agriculture College of Life Sciences Nanjing Agricultural University Nanjing 210095 (China)

A bacterial strain, pcnb-21, capable of degrading pentaehloronitrobenzene （PCNB） under aerobic and anoxic conditions, was isolated from a long-term PCNB-polluted soil by an enrichment culture technique and identified as Labrys portucalensis based upon its morphological, physiological and biochemical properties, as well as 16S rRNA gene sequence analysis. Effects of different factors, such as temperature and pH, on PCNB biodegradation were studied. Strain pcnb-21 efficiently degraded PCNB at temperatures from 20 to 30 ℃ and initial pH values from 4 to 7, which might be the first time that a Labrys strain was found capable of eflClciently degrading PC1NB. The degradation of PCNB was affected by oxygen, and the degradation decreased with increasing aeration. Exogenous electron donors such as glucose, lactic acid and succinic acid promoted the biodegradation of PCNB, while electron acceptors such as sodium nitrite, sodium sulfate, sodium nitrate and sodium sulfate inhibited PCNB biodegradation. The degradation of PCNB in sterile and non-sterile soils by a green fluorescent protein （GFP）-labeled strain, pcnb-21-gfp, was also studied. Cells of pcnb-21-gfp efficiently degraded 100 mg kg-1 PCNB in sterile and non-sterile soils and could not be detected after 42 days. Strain pcnb-21 might be useful in bioremediating PCNB-polluted soils and environment.

关键词： aeration bacterial strain electron acceptors electron donors sterile soil

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