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QTL analysis of early flowering of female flowers in zucchini(Cucurbita pepo L.)

Journal of Integrative Agriculture 2023年第11期22卷 3321-3330页

作者： QU Shu-ping YANG Dan YU Hai-yang CHEN Fang-yuan WANG Ke-xin DING Wen-qi XU Wen-long WANG Yun-li Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Northeast Region) Ministry of Agriculture and Rural Affairs/College of Horticulture and Landscape ArchitectureNortheast Agricultural UniversityHarbin 150030P.R.China Laboratory of Molecular Breeding of Pumpkin College of Horticulture and Landscape ArchitectureNortheast Agricultural UniversityHarbin 150030P.R.China

early flowering promotes early maturity, production, and the capacity to counteract biotic and abiotic stresses, making it an important agronomic trait in zucchini. The present study demonstrated that the zucchini inbred line ‘19’ consistently flowered early, taking significantly fewer days to bloom the first female flower(DFF) than the inbred line ‘113’. Genetic analysis revealed that DFF, an inheritable quantitative trait, is controlled by multiple genes. Based on the strategy of quantitative trait locus(QTL) sequencing(QTL-seq) combined with linkage analysis, three QTLs for DFF were identified on chromosomes 4, 11, and 20. This study used additional F2 populations grown under different environmental conditions for QTL mapping analysis of DFF with insertion/deletion(InDel) markers to validate these results. Using the composite interval mapping(CIM) method of R/qtl software, we only identified one major locus under all environmental conditions, located in a 117-kb candidate region on chromosome 20. Based on gene annotation, gene sequence alignment, and qRT-PCR analysis, we found that the Cp4.1LG20g08050 gene encoding a RING finger protein may be a candidate gene for the opposite regulation of early flowering in zucchini. In summary, these results lay a foundation for a better understanding of early flowering and improving early flowering-based breeding strategies in zucchini.

关键词： Cucurbita pepo L. early flowering days to bloom the first female flower QTL analysis

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Ecotopic over-expression of an FT-homologus gene of Gossypium hirsutum induces early flowering in Arabidopsis thaliana and Nicotiana tabacum

Ecotopic over-expression of an FT-homologus gene of Gossypiu...

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从植物科学到农业发展——2012全国植物生物学大会

作者： Xianzhong Huang~* Rui Dong,Chao Gu,Chao Li,Feng Zhang,Baiming Cui Key Laboratory of Agrobiotechnology,College of Life Science,Shihezi University,Shihezi,xinjiang, 832003,China.

Cloning and function research of FT(flowering locus T)-like homo logs in cotton haven’t been *** the present study,an FT-Like homolog was isolated from Gossypium hirsutum *** RT-PCR and RACE,which was designated GhFTL1 *** entire coding region is 525 bp and encoded a polypeptide of 174 amino acids with 75%homology to AtFT and 84%to Hd3a,*** protein contains two critical residues tyrosine(Tyr) and glutamine(Gln),which identified as diagnostic of true FT family *** analysis and 3-dimensional structure indicated GhFTLl gene belongs to FT-like homologs. GhFTL1 gene showed constitutive expression in *** expression of GhFTL1 was regulated by photoperiod,with the highest expression from the 10th to 12th hour of the light period and the lowest expression at dawn,which is similar to FT in Arabidopsis. Over-expression of GhFTL1 not only in Arabidopsis thaliana but also in Nicotiana tabacum using the CaMV 35S promoter resulted in significant accelerated flowering compared with wild-type ***1 played an important role in flowering regulation of *** function of further research on GhFTL1 is now underway.

关键词： Florigen GhFTL1 flowering LOCUST early flowering Gossypium hirsutum

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Evolutionary assessment of SQUAMOSA PROMOTER BINDING PROTEIN‑LIKE genes in citrus relatives with a specific focus on flowering

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Molecular Horticulture 2023年第1期3卷 214-229页

作者： Yawei Li Shuting Wang Prakash Babu Adhikari Bing Liu Shengjun Liu Yue Huang Gang Hu Michitaka Notaguchi Qiang Xu National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops Huazhong Agricultural UniversityWuhan 430000China Bioscience and Biotechnology Center Nagoya UniversityFuro‑choChikusa‑kuNagoya 464‑8601Japan Hubei Hongshan Laboratory Wuhan 430070China

Phase transition and floral induction in citrus requires several years of juvenility after *** a long period of juvenility has been a major hindrance to its genetic improvement *** have shown that miR156 along with its downstream genes SQUAMOSA PROMOTER BINDING PROTEINS(SBP)and SBP-LIKE(SPL)mediate the phase transition and floral induction process in *** current study has systematically analyzed SPLs in 15 different citrus-related species,systematically annotated them based on their close homology to their respective Arabidopsis orthologs,and confirmed the functional attributes of the selected members in floral *** majority of the species harbored 15 *** cis-element assessment suggested the involvement of the SPLs in diverse developmental and physiological processes in response to different biotic and abiotic *** all,SPL5,SPL9,and SPL11 stood out as consistently differentially expressed SPLs in the adult and young tissues of different citrusrelated *** overexpression of their *** orthologs(FhSPL5,FhSPL9,and FhSPL11)brought an enhanced expression of endogenous flowering LOCUS T leading to the significantly precocious flowering in transgenic Arabidopsis *** study of the genes in the citrus plant itself is expected to conclude the assessments made in the current study.

关键词： SPL Gene Family Phylogeny Phase Transition early flowering

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Temporal and Spatial Requirement of EMF1 Activity for Arabidopsis Vegetative and Reproductive Development

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Molecular Plant 2009年第4期2卷 643-653页

作者： Rosario Sanchez Minjung Y. Kim Myriam Calonje Yong-Hwan Moon Z. Renee Sung Department of Plant and Microbial Biology University of California Berkeley CA 94720 USA Present address: Heidelberg Institute for Plant Science Biodiversity and Plant Systematics Heidelberg University 69120 Heidelberg Germany Present address: Department of Molecular Biology Pusan National University 30 Jangjeon-dong Geumjeong-gu Busan 609-735 Korea

EMBRYONIC FLOWER （EMF） genes are required to maintain vegetative development via repression of flower homeotic genes in Arabidopsis. Removal of EMF gene function caused plants to flower upon germination, producing abnormal and sterile flowers. The pleiotropic effect of ernfl mutation suggests its requirement for gene programs involved in diverse developmental processes. Transgenic plants harboring EMF1 promoter：：glucuronidase （GUS） reporter gene were generated to investigate the temporal and spatial expression pattern of EMF1. These plants displayed differential GUS activity in vegetative and flower tissues, consistent with the role of EMF1 in regulating multiple gene programs. EMFI：：GUS expression pattern in emf mutants suggests organ-specific auto-regulation. Sense- and antisense （as） EMF1 cDNA were expressed under the control of stage- and tissue-specific promoters in transgenic plants. Characterization of these transgenic plants showed that EMF1 activity is required in meristematic as well as differentiating tissues to rescue emf mutant phenotype. Temporal removal or reduction of EMF1 activity in the embryo or shoot apex of wild-type seedlings was sufficient to cause early flowering and terminal flower formation in adult plants. Such reproductive cell memory is reflected in the flower MADS-box gene activity expressed prior to flowering in these early flowering plants. However, temporal removal of EMF1 activity in flower meristem did not affect flower development. Our results are consistent with EMF1＇s primary role in repressing flowering in order to allow for vegetative growth.

关键词： EMF1 stage-specific promoter early flowering reproductive cell memory vegetative/reproductive development repression of flower MADS-box genes.

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Transformation of Tobacco with Two AP1 Genes Isolated from Poplar( Populus simonii × Populus nigra)

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Agricultural Biotechnology 2014年第4期3卷 23-27页

作者： Shuang LI Tangchun ZHENG Lina ZANG Guanzheng QU State Key Laboratory of Tree Genetics and Breeding Northeast Forestry University

[ Objective ] This study aimed to analyze the functions of AP1 gene from Populus simonii × Populus nigra and to lay the theoretical foundation for shortening the breeding cycle of forest trees and investigating the flowering mechanism in poplar. [ Method] Plant expression vectors of AP1 genes were constructed and transformed into tobacco leaf disks with Agrobacterium-mediated method. Transgenic tobacco plants were identified by PCR. [ Result] AP1 genes were integrated into the genome of tobacco. Transgenic tobacco plants all presented an early flowering phenotype compared with wild-type tobacco. [ Conclusion] AP1 genes could promote early flowering in transgenic tobacco plants, which provided theoretical basis for molecular regulation of flowering in poplar.

关键词： Populus simonii ×Popul nigra AP1 Tobacco early flowering

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