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Aerobic exercise suppresses hepatocellular carcinoma by downregulating dynamin-related protein 1 through PI3K/AKT pathway

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Journal of Integrative Medicine 2021年第5期19卷 418-427页

作者： Tong Zhao Bing-jie Guo Chu-lan Xiao Jiao-jiao Chen Can Lü Fan-fu Fang Bai Li School of Traditional Chinese Medicine Naval Medical UniversityShanghai 200433China Nanjing University of Chinese Medicine.Nanjing 210023 Jiangsu ProvinceChina

Objective: Exercise, as a common non-drug intervention, is one of several lifestyle choices known to reduce the risk of cancer. Mitochondrial division has been reported to play a key role in the occurrence and transformation of hepatocellular carcinoma(HCC). This study investigated whether exercise could regulate the occurrence and development of HCC through ***: Bioinformatics technology was used to analyze the expression level of dynamin-related protein1(DRP1), a key protein of mitochondrial division. The effects of DRP1 and DRP1 inhibitor(mdivi-1) on the proliferation and migration of liver cancer cells BEL-7402 were observed using cell counting kit-8, plate colony formation, transwell cell migration, and scratch experiments. Enzyme-linked immunosorbent assay, Western blot and real-time polymerase chain reaction were used to detect the expression of DRP1 and its downstream phosphoinositide 3-kinase(PI3 K)/protein kinase B(AKT) pathway. A treadmill exercise intervention was tested in a nude mouse human liver cancer subcutaneous tumor model expressing different levels of DRP1. The size and weight of subcutaneous tumors in mice were detected before and after ***: The expression of DRP1 in liver cancer tissues was significantly upregulated compared with normal liver tissues(P<0.001). The proliferation rate and the migration of BEL-7402 cells in the DRP1 overexpression group were higher than that in the control group. The mdivi-1 group showed an inhibitory effect on the proliferation and migration of BEL-7402 cells at 50 lmol/L. Aerobic exercise was able to inhibit the expression of DRP1 and decrease the size and weight of subcutaneous tumors. Moreover,the expression of phosphorylated PI3 K(p-PI3 K) and phosphorylated AKT(p-AKT) decreased in the exercise group. However, exercise could not change p-PI3 K and p-AKT levels after knocking down DRP1 or using mdivi-1 on subcutaneous ***: Aerobic exercise can suppress the development of

关键词： Aerobic exercise Hepatocellular carcinoma dynamin-related protein PI3K/AKT pathway

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Differential Roles of Arabidopsis dynamin-related proteins DRP3A,DRP3B,and DRP5B in Organelle Division

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Journal of Integrative Plant Biology 2012年第11期54卷 921-931页

作者： Kyaw Aung MSU-DOE Plant Research Laboratory and Plant Biology Department Michigan State UniversityEast LansingMI 48824USA

dynaminrelated proteins （DRPs） are key components of the organelle division machineries, functioning as molecular scissors during the fission process. In Arabidopsis, DRP3A and DRP3B are shared by peroxisomal and mitochondrial division, whereas the structurallydistinct DRP5B （ARC5） protein is involved in the division of chloroplasts and peroxisomes. Here, we further investigated the roles of DRP3A, DRP3B, and DRP5B in organelle division and plant development. Despite DRP5B＇s lack of stable association with mitochondria, drp5B mutants show defects in mitochondrial division.

关键词： Arabidopsis dynamin-related protein mitochondria.

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