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Smoothness preserving layout for dynamic labels by hybrid optimization

Computational Visual Media 2022年第1期8卷 149-163页

作者： Yu He Guo-Dong Zhao Song-Hai Zhang Department of Computer Science and Technology Tsinghua UniversityBeijingChina Department of Computer Science and Technology Tianjin University of TechnologyTianjinChina

Stable label movement and smooth label trajectory are critical for effective information *** label changes cannot be avoided by whatever forced directed methods due to the unreliability of resultant force or global optimization methods due to the complex trade-off on the different *** solve this problem,we proposed a hybrid optimization method by taking advantages of the merits of both *** first detect the spatial-temporal intersection regions from whole trajectories of the features,and initialize the layout by optimization in decreasing order by the number of the involved *** label movements between the spatial-temporal intersection regions are determined by force directed *** cope with some features with high speed relative to neighbors,we introduced a force from future,called temporal force,so that the labels of related features can elude ahead of time and retain smooth *** also proposed a strategy by optimizing the label layout to predict the trajectories of features so that such global optimization method can be applied to streaming data.

关键词： label layout smoothness preserving dynamic label forced based static optimization hybrid optimization

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The specific and rapid labeling of cell surface proteins with recombinant FKBP-fused fluorescent proteins

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Protein & Cell 2014年第10期5卷 800-803页

作者： Xi Zhang Yongqiang Deng Hao Chang Chen Ji Mingshu Zhang Jianxin Peng Tao Xu Pingyong Xu Institute of Entomology School of Life Sciences Central ChinaNormal University Wuhan 430079 China National Key Laboratory of Biomacromolecules Institute ofBiophysics Chinese Academy of Sciences Beijing 100101 China School of Life Sciences University of Science and Technology ofChina Hefei 230026 China Laboratory of interdisciplinary Research Institute of BiophysicsChinese Academy of Sciences Beijing 100101 China

Dear Editor, The presence and abundance of proteins in the plasma membrane （PM） is regulated by exocytosis and endocytosis. Exocytosis is required for the maintenance of cellular homeostasis following stimulation, whereas endocytosis is important for the reuse and degradation of PM and receptor proteins. Through the specific labeling of proteins on the PM, the process of endocytosis can be tracked and monitored in living cells. Several genetic tags and site-specific labeling approaches that involve the coupling of small organic mol- ecules to tag-fused proteins through either self-labeling or enzymatic ligation have been developed for this purpose （Chen et al., 2005; George et al., 2004; Hoffmann et al.,

关键词： Cell Surface Protein label Efficiency Ascomycin Retrograde Trafficking dynamic label

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