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Protection of MES23.5 dopaminergic cells by obestatin is mediated by proliferative rather than anti-apoptotic action

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Neuroscience Bulletin 2014年第1期30卷 118-124页

作者： Xiao-Li Shen Feng-Ju Jia Ning Song Jun-Xia Xie Hong Jiang Department of Physiology Shandong Provincial Key Laboratory of Pathogenesis and Prevention of Neurological Disorders and State Key Disciplines: Physiology Medical College of Qingdao University

Obestatin is an endogenous peptide sharing a precursor with ghrelin. This study aims to investigate whether and how obestatin protects MES23.5 dopaminergic cells against 1-methyl-4- phenylpyridinium （MPP^＋）-induced neurotoxicity. MES23.5 cells were pretreated with obestatin （10^-13- 10^-6 mol/L） for 20 min prior to incubation with 200 μmol/L MPP^＋ for 12 or 24 h, or treated with obestatin alone （10^-13 to 10^-6 mol/L） for 0, 6, 12, and 24 h. The methyl thiazolyl tetrazolium （MTT） assay was used to measure cell viability. Flow cytometry was used to measure the caspase-3 activity and the mitochondrial transmembrane potential. Proliferating cell nuclear antigen （PCNA） protein levels were determined by Western blotting. Obestatin （10^-13 to 10^-7 mol/L） pretreatment blocked or even reversed the MPP^＋ induced reduction of viability in MES23.5 cells, but had no effect on MPP^＋-induced mitochondrial transmembrane potential collapse and caspase-3 activation. When applied alone, obestatin increased viability. Elevated PCNA levels occurred with 10^-7, 10^-9, 10^-11 and 10^-13 mol/L obestatin treatment for 12 h. The results suggest that the protective effects of obestatin against MPP^＋ in MES23.5 cells are due to its proliferation-promoting rather than anti-apoptotic effects.

关键词： obestatin MPP^＋ proliferation apoptosis dopaminergic cells

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Protective effects of obestatin are mediated by proliferative rather than anti-apoptotic properties in MES23.5 dopaminergic cells

Protective effects of obestatin are mediated by proliferativ...

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中国神经科学学会第十届全国学术会议

作者： Xiao-Li SHEN Ning SONG Jun-Xia XIE Hong JIANG Department of Physiology Shandong Provincial Key Laboratory of Pathogenesis and Prevention of Neurological Disorders and State Key Disciplines:PhysiologyMedical College of Qingdao University

Objective To test whether obestatin,an endogenous peptide shares the same peptide precursor with ghrelin,has neuroprotective effects on dopaminergic cells against 1-methyl-4-phenylpyridinium(MPP)-induced MES23.5 cells neurotoxicity and the mechanism of it.Methods The protective effects of obestatin(100 mol/L) against MPP-induced MES23.5 cells neurotoxicity and the proliferative effects of obestatin(100 mol/L)were tested by methyl thiazolyl tetrazolium(MTT) assay.Flow cytometry was used to measure the changes of caspase-3 activity and the mitochondrial membrane potential with obestatin(100 mol/L) pretreatments in MES23.5 cells.Results Obestatin(100 mol/L) pretreatment could reverse 1-methyl-4-pheylpyrifinium(MPP)-induced reductions in in dopaminergic MES23.5 cells’ viability,viability.However,it had no effects on MPP-induced mitochondrial transmembrane potential collapse and caspase-3 activation.Obestatin(10 10 mol/L) itself could cause MES23.5cell proliferation.Conclusion The protective effects of obestatin in MES23.5 cells were due to its proliferative rather than anti-apoptotic effects.

关键词： obestatin MPP+ proliferation apoptosis dopaminergic cells

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