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Coexistence of dopamine-beta-hydroxylase and activated protein-2 alpha in rat cerebellar Purkinje cells

Neural Regeneration Research 2009年第6期4卷 431-435页

作者： Kejian Wang Wei Li Shanquan Sun Zhongqin Ren Guiqiong He Department of Human Anatomy Coflege of Basic Medicine Chongqing Medical University Chongqing 400016 China Institute of Neuroscience Chongqing Medical University Chongqing 400016 China

BACKGROUND： Tyrosine hydroxylase and phenylethanolamine-n-methyl transferase expression coexist in Purkinje cells of the rat cerebellum. Numerous reports have also been published addressing whether dopamine-beta-hydroxylase （DBH） expression exists in cerebellar Purkinje cells. OBJECTIVE： To investigate the coexistence of DBH and activator protein-2α expression in rat cerebellar Purkinje cells. DESIGN, TIME AND SETTING： A cell morphological study was performed at the Institute of Neuroscience, Chongqing Medical University, China in May 2007. MATERIALS： Ten healthy Wistar rats, of either gender, aged 14 weeks, served as experimental animals. Rabbit anti-mouse DBH, goat anti-mouse activator protein-2α and rabbit anti-mouse β-actin （Santa Cruz Biotechnology, Inc., USA）, horseradish peroxidase-labeled goat anti-rabbit IgG, FITC-labeled mouse anti-rabbit IgG, and Cy3-labeled mouse anti-goat IgG （Boster, Wuhan, China）, were used in this study. METHODS： Immunohistochemical staining was used to measure the expression of DBH or activator protein-2α, with double-label immunofluorescence being employed to determine coexpression of both, in the cerebellum of 5 randomly selected rats. Western blot assay was utilized to determine the expression of DBH and activator protein-2α in the cerebellum of the remaining 5 rats. MAIN OUTCOME MEASURES： Expression, localization and coexistence of DBH and activator protein-2α in the cerebellum were measured separately. RESULTS： Immunohistochemical staining demonstrated that cerebellar Purkinje cells stained positive for DBH and activator protein-2α. Western blot assay also demonstrated DBH and activator protein-2α expression in the cerebellum. Double-labeling immunofluorescence showed the coexistence of DBH and activator protein-2α in cerebellar Purkinje cells. CONCLUSION： Norepinephrine and activator protein-2α coexist in rat cerebellar Purkinje cells.

关键词： Purkinje cells cerebellum dopamine-beta-hydroxylase activator protein-2α

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Acupuncture Improves Withdrawal Symptoms by Inhibiting Tyrosine hydroxylase and dopamine-beta-hydroxylase Expression in the Midbrain of Heroin Re-addicted Rats

Acupuncture Improves Withdrawal Symptoms by Inhibiting Tyros...

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新时代新思维新跨越新发展——2019中国针灸学会年会暨40周年回顾

作者： ZhuYue Chen Kun Wang XiaoGe Song Xing HuiCai XiaoRong Hou LiDa Zhang RongJun Zhang GuoQi Zhu ShengBing Wu Acupuncture and Massage College Anhui University of Traditional Chinese Medicine Scientific Research Experiment Center Anhui University of Chinese Medicine Institute of Acupuncture-Moxibustion and Meridian Anhui Academy of Traditional Chinese Medicine Institute of Integrated Chinese and Western Medicine Anhui University of Traditional Chinese Medicine Key Laboratory of Xin’an Medicine Ministry of EducationAnhui University of Chinese Medicine

We studied the effects of acupuncture on catecholamine neurotransmitter regulation in the context of withdrawal symptoms. Sprague-Dawley rats were treated with three cycles of addiction and withdrawal to establish a heroin re-addicted rat model. In the withdrawal period, rats in the acupuncture group were given acupuncture at the Baihui and Dazhui *** day 37 of the experiment, we measured tyrosine hydroxylase(TH) and dopamine-beta-hydroxylase(DβH) expression in the ventral tegmental area(VTA) of the midbrain, and compared the levels of dopamine(DA), norepinephrine(NE), and5-hydroxytryptamine(5-HT) in the VTA and serum of the experimental groups. The acupuncture group had significantly lower 48-and 72-hour scores of withdrawal symptoms(P < 0.05) than did the model group. The model group had significantly higher levels of DA, NE, and 5-TH in the VTA than did the control group(P < 0.01), and the acupuncture group had significantly lower levels of DA, NE, and 5-TH than did the model group(P < 0.01). The model group showed significantly higher TH and DβH expression in the VTA than did the control group(P < 0.01),and the acupuncture group had significantly higher TH and DβH expression than the model group(P < 0.01). At 48 and 72 hours after heroin withdrawal, the withdrawal symptoms scores were positively correlated with DA, NE, and 5-HT levels and TH and DβH expression in the VTA. Acupuncture could therefore improve withdrawal symptoms by regulating DA, NE, and5-HT expression by inhibiting TH and DβH.

关键词： Acupuncture Heroin re-addicted rat Ventral tegmental area of the midbrain Tyrosine hydroxylase dopamine-beta-hydroxylase Catecholamine neurotransmitter Withdrawal symptom

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