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Effect of Different Reproductive Stages and Culture Times on domestic cat <i>in Vitro </i>Oocyte Maturation

Advances in Reproductive Sciences 2021年第2期9卷 129-138页

作者： José Ernesto Hernández Pichardo Miguel Ramses Del Moral Reyes Michael E. Kjelland José Luis Rodríguez Suastegui Department of Agricultural and Animal Production Division of Biological and Health SciencesMetropolitan Autonomous University-Xochimilco Mexico City Mexico Private Clinic Mexico City Mexico Conservation Genetics & Biotech LLC Valley City USA Biological and Health Sciences PhD Program Metropolitan Autonomous University-Xochimilco Mexico City Mexico

The domestic cat has been used as a model to carry out comparative research in assisted reproduction, to be applied in wild cats. The efficiency in domestic cat IVM concerning the reproductive status and/or cultivation times has previously been investigated;however, the studies were carried out separately. The objective of this research was to evaluate the maturation of oocytes of domestic cats of different reproductive stages using two different in vitro culture times. The ovaries were obtained by Ooforo-Salpingo-Hysterectomy of cats that were of the following groups: 1) prepubertal, 2) follicular, 3) pregnant or 4) in anestrus. Maturation was carried out with TCM199 medium supplemented with BSA for 24 h and 48 h. On average, 29 ± 25, 20 ± 15, 17 ± 9 and 17 ± 13 oocytes/cat were recovered from the prepubertal follicular, pregnant, and anestrus stages, respectively, but did not show a significant difference (P > 0.05). Also, meiotic maturation did not show a significant difference between the different reproductive stages at 24 h and 48 h, respectively (P > 0.05). However, in the prepubertal and follicular stages, greater oocyte maturation numbers were observed at 48 h compared to 24 h (P result was not observed in the pregnant and anestrus stages (P > 0.05), indicating that the in vitro culture duration is an important factor during in vitro maturation of domestic cat oocytes.

关键词： domestic cat Oocytes In Vitro Maturation Culture Time Estrus Cycle Stages

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Effect of Duration on Maturation in vitro of domestic cat Oocytes

Effect of Duration on Maturation in vitro of Domestic Cat Oo...

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中国畜牧兽医学会兽医产科学分会第五届全体会议第十次学术研讨会

作者： WU Jing~(1,2),GUO Yong~1,WANG Xin-zhuang~(3,2),LIU Yun-hai~1,LI Kai~1,BAO Wen-yu~1,XU Yang~1,NI He-min~(1*) (1.Department of Animal Science and Technology,Beijing University of Agriculture,Beijing 102206,China 2.College of Veterinary Medicine,Northwest A&F University,Yangling 712100,China 3.Henan University of Agriculture,Zhengzhou 450002,China)

The aim of this study is to investigate the effect of durations on nuclear maturation in vitro of feline oocytes. Following the in vitro maturation(IVM) in Medium 199 added with FSH and *** oocytes were treated for 28h.32h and 36h *** vitro maturated oocytes were subjected to artifical activation in 10%*** activated oocytes were cultured 6 *** rate of activated oocytes was evaluated on Day *** were stained with Hoechest 33342 on Day 6 to observe the potential of activated *** the same time,the matured oocytes were fixed in 3.7% paraformaldehyde,stained in FITC-LCA and *** observed the distribution of cortical granules(CGs) to evaluate the cytoplasmic *** cleave rate of activated oocytes in the 32h group was significantly higher than the 28h group (60.42%vs 43.16%.0.010.05).After activation,there was no oocyte which developed to *** rate of morula in the 32h group was highest,but there was no significant differences among 28 h.32 h.36 h(7.37%,15.63%vs 10.78%,P>0.05). CGs were found throughout the oocyte *** were more CGs located in the cortex of the oocytes which were matured 32h than the other *** results showed that the optimal maturation duration of feline oocytes was 32h.

关键词： domestic cat oocyte duration of lVM parthenogenetic activation cortical granules

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Optimal Center-of-Mass Pivot Effect of Spinal Motion in Feline Galloping

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Journal of Bionic Engineering 2020年第5期17卷 970-977页

作者： Young Kook Kim Dongwon Yun LV R&D team Electro-technology R&D CenterLS ELECTRIC Co.LtdCheongju 28437South Korea Department of Robotics Engineering Daegu Gyeongbuk Institute of Science and Technology(DGIST)Daegu 42988South Korea

In this paper,we studied the acceleration behavior of a quadruped animal during a galloping *** the development of many quadruped robotic systems has been focused on dynamic movements,it is obvious that guidance from the dynamic behavior of quadruped animals is needed for robotics *** fulfill this demand,this paper deals with analysis of the galloping motions of a domestic cat,which is well known for its excellent acceleration performance among four-legged *** on the planar motion capture environment,the movement data of a galloping feline was acquired and the dynamic motions were estimated using a spring-mass *** particular,the effects of the position and angle of the center-of-mass of the cat,angular displacement of the spine,and angular velocity of the spine were analyzed and are discussed *** this process,it was possible to understand the dynamic movement characteristics of the cat,and to understand the relationships between,and the influences of,these *** this analysis,we provide significant data applicable to the design of joint movements in quadruped robot systems.

关键词： quadrupedalism feline galloping domestic cat center-of-mass

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