检索结果-南通市图书馆

Endogenous biosynthesis of docosahexaenoic acid(DHA)regulates fish oocyte maturation by promoting pregnenolone production

引用

Zoological Research 2024年第1期45卷 176-188页

作者： Yi Li Xuehui Li Ding Ye Ru Zhang Chengjie Liu Mudan He Houpeng Wang Wei Hu Yonghua Sun State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyInnovation Academy for Seed DesignChinese Academy of SciencesWuhanHubei 430072China College of Advanced Agricultural Sciences University of Chinese Academy of SciencesBeijing 100049China Hubei Hongshan Laboratory WuhanHubei 430070China

Omega-3 polyunsaturated fatty acids(n-3 PUFAs),particularly docosahexaenoic acid(22:6n-3,DHA),play crucial roles in the reproductive health of vertebrates,including ***,the underlying mechanism related to this phenomenon remains largely *** this study,we employed two zebrafish genetic models,i.e.,elovl2^(-/-)mutant as an endogenous DHAdeficient model and fat1(omega-3 desaturase encoding gene)transgenic zebrafish as an endogenous DHA-rich model,to investigate the effects of DHA on oocyte maturation and *** show that the elovl2^(-/-)mutants had much lower fecundity and poorer oocyte quality than the wild-type controls,while the fat1 zebrafish had higher fecundity and better oocyte quality than wildtype *** deficiency in elovl2^(-/-)embryos led to defects in egg activation,poor microtubule stability,and reduced pregnenolone *** study revealed that DHA promoted pregnenolone synthesis by enhancing transcription of cyp11a1,which encodes the cholesterol side-chain cleavage enzyme,thereby stabilizing microtubule assembly during *** turn,the hypothalamic-pituitary-gonadal axis was enhanced by *** conclusion,using two unique genetic models,our findings demonstrate that endogenously synthesized DHA promotes oocyte maturation and quality by promoting pregnenolone production via transcriptional regulation of cyp11a1.

关键词： docosahexaenoic acid Oocyte maturation Oocyte quality Pregnenolone Microtubule

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

docosahexaenoic acid suppresses arachidonic acid-induced proliferation of LS-174T human colon carcinoma cells

引用

World Journal of Gastroenterology 2009年第9期15卷 1079-1084页

作者： Piet Habbel Karsten H Weylandt Katja Lichopoj Johannes Nowak Martin Purschke Jing-Dong Wang Cheng-Wei He Daniel C Baumgart Jing X Kang Department of Medicine Massachusetts General Hospital and Harvard Medical School Boston MA 02114 United States Department of Medicine Division of Gastroenterology and Hepatology Charité Medical Center-Virchow-Hospital Medical School of the Humboldt-University of Berlin 13344 Berlin Germany

AIM： To investigate the impact of arachidonic acid （AA） and docosahexaenoic acid （DHA） and their combination on colon cancer cell growth. METHODS： The LS-174T colon cancer cell line was used to study the role of the prostaglandin precursor AA and the omega-3 polyunsaturated fatty acid DHA on cell growth. Cell viability was assessed in XTT assays. For analysis of cell cycle and cell death, flow cytometry and DAPI staining were applied. Expression of cyclooxygenase-2 （COX-2）, p21 and bcl-2 in ceils incubated with AA or DHA was examined by real-time RT-PCR. Prostaglandin E2 （PGE2） generation in the presence of AA and DHA was measured using a PGE2- ELISA. RESULTS： AA increased cell growth, whereas DHA reduced viability of LS 174T cells in a time- and dosedependent manner. Furthermore, DHA down- regulated mRNA of bcl-2 and up-regulated p21. Interestingly, DHA was able to suppress AA-induced cell proliferation and significantly lowered AA-derived PGE2 formation. DHA also down-regulated COX-2 expression. In addition to the effect on PGE2 formation, DHA directly reduced PGE2-induced cell proliferation in a dosedependent manner. CONCLUSION： These results suggest that DHA can inhibit the pro-proliferative effect of abundant AA or PGE2.

关键词： Colorectal carcinoma Colon cancer Omega-3 Omega-6 Polyunsaturated fatty acids Arachidonic acid docosahexaenoic acid ProstaglandinE2 Cyclooxygenase-2 Apoptosis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

docosahexaenoic acid-rich fish oil prevented insulin resistance by modulating gut microbiome and promoting colonic peptide YY expression in diet-induced obesity mice

引用

Food Science and Human Wellness 2022年第1期11卷 177-188页

作者： Wanxiu Cao Fang Liu Robert WLi Yaoxian Chin Yuming Wang Changhu Xue Qingjuan Tang College of Food Science and Engineering Ocean University of ChinaQingdao 266003China Key Laboratory of Marine Drugs Ministry of EducationSchool of Medicine and PharmacyOcean University of ChinaQingdao 266003China United States Department of Agriculture Agriculture Research Service(USDA-ARS)Animal Genomics and Improvement LaboratoryBeltsvilleMD 20705USA Hainan Tropical Ocean University Sanya 572022China Laboratory for Marine Drugs and Bioproducts Qingdao National Laboratory for Marine Science and TechnologyQingdao 266003China

It is unclear how docosahexaenoic acid(DHA)improves insulin resistance via modulating gut microbiome in obese *** used diet-induced obesity(DIO)mice as a model to study the effects of DHA-rich fish oil(DHA-FO)on host metabolic disorders and colonic ***-FO reduced fat deposition,regulated lipid profiles and alleviated insulin resistance in DIO *** because DHA-FO prevented the permeation of lipopolysaccharide across intestinal epithelial barrier,and promoted peptide YY(PYY)secretion via the mediation of short chain fatty acids receptor(FFAR2)in ***,DHA-FO might regulate PYY expression by reversing microbial dysbiosis,including increasing the abundance ofAkkermansia muciniphila and Lactobacillus,and suppressing the growth of ***-FO also altered gut microbial function(e.g."linoleic acid metabolism")associated with PYY expression(r>0.80,P<0.05).Herein,DHA-FO enhanced insulin action on glucose metabolism by altering gut microbiome and facilitating colonic PYY expression in DIO mice.

关键词： docosahexaenoic acid Diet-induced obesity Insulin resistance Peptide YY Gut microbiome

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

High Sn-2 docosahexaenoic acid Lipids for Brain Benefits, and Their Enzymatic Syntheses: A Review

引用

Engineering 2020年第4期6卷 424-431页

作者： Jun Jin Qingzhe Jin Xingguo Wang Casimir CAkoh Department of Food Science and Technology The University of GeorgiaAthensGA 30602USA International Joint Research Laboratory for Lipid Nutrition and Safety State Key Laboratory of Food Science and TechnologySchool of Food Science and TechnologyJiangnan UniversityWuxi 214122China

The normal development and maintenance of central neural functions are highly correlated with the amount of docosahexaenoic acid(DHA;ω-3 fatty acid)accumulated in the *** incorporated at the sn-2 position of lipids is well absorbed by intestinal mucosa and utilized efficiently in ***,modern consumers have a reduced direct intake of DHA and increased intake of saturated fats or ω-6 fatty acid oils,resulting in behavioral and neurophysiological *** provide an understanding of the integrated beneficial effects of DHA on the human brain,this review introduces the positional difference(sn-2 and sn-1,3 positions)of DHA on a glycerol skeleton in natural fats and oils,and further discusses the possible functional mechanism regarding DHA supplementation and the gut-brain *** multiple bidirectional routes in this axis offer a novel insight into the interaction between DHA supplementation,the gut microbiota,and brain *** achieve high sn-2 DHA in diets,it is suggested that sn-2 DHA lipids be enzymatically produced in more efficient and economical ways by improving the specific activities of lipases and optimizing the purification *** types of diets will benefit individuals with strong needs for sn-2 ω-3 lipids such as infants,children,and pregnant and lactating women.

关键词： docosahexaenoic acid Sn-2 docosahexaenoic acid Monoacylglycerol Brain Gut-brain axis Structured lipid

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Fatty acid Composition and Digestive Enzyme Activities of Rainbow Trout in Response to Dietary docosahexaenoic acid(DHA)and Eicosapentaenoic acid(EPA)During Salinity Acclimation

引用

Journal of Ocean University of China 2020年第6期19卷 1430-1440页

作者： HUANG Ming ZHOU Yangen GE Jian AGUSTSSON Thorleifur LI Li GAO Qinfeng DONG Shuanglin Key Laboratory of Mariculture(Ocean University of China) Ministry of EducationQingdao 266100China NORCE Norwegian Research Center Mekjarvik 124072RandabergNorway RORUM Brynjolfsgata 5 107ReykjavikIceland Function Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyQingdao 266235China

This physiological study aimed to evaluate the effects of dietary docosahexaenoic acid(DHA)and eicosapentaenoic acid(EPA)on the fatty acid composition and digestive enzyme activities of rainbow trout(Oncorhynchus mykiss)during salinity *** trout with an average initial weight of 90.61 g±9.25 g were fed diets with the quantities of DHA and EPA equaling to 0.54%,0.95%,1.40%and 1.79%(abbreviated as DE-0.54,DE-0.95,DE-1.40,and DE-1.79,respectively)for eight weeks,after which the gastric and intestinal fatty acids composition were ***,the fish underwent salinity *** days 1,4,7,and 14 after the freshwater was replaced by seawater and at the end of the 8-week period,gastric and intestinal digestive enzyme activities were *** results showed that the gastric and intestinal DHA and EPA contents of the fish were positively correlated to their dietary DHA and EPA *** dietary DHA and EPA levels inhibited the protease activity of rainbow *** in the DE-0.54 group increased the lipase activity to enhance the utilization of lipids maybe due to the inadequate essential fatty acids for fish in this ***,rainbow trout in the DE-0.54 group failed to maintain suitable activities of digestive enzymes after salinity ***,a diet with minimum 0.95%DHA and EPA levels is necessary for rainbow trout during salinity acclimation.

关键词： digestive enzymes docosahexaenoic acid eicosapentaenoic acid rainbow trout

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Encapsulation of docosahexaenoic acid (DHA) using self-assembling food-derived proteins for efficient biological functions

引用

Food Science and Human Wellness 2023年第5期12卷 1861-1871页

作者： Yumeng Liu Kaixin Cao Tong Li Delun Mu Naiqi Zhang Ying Wang Rina Wu Junrui Wu College of Food Science Shenyang Agricultural UniversityShenyang 110866China Liaoning Engineering Research Center of Food Fermentation Technology Shenyang 110161China Shenyang Key Laboratory of Microbial Fermentation Technology Innovation Shenyang 110866China

docosahexaenoic acid(DHA;22n-6)possesses multiple biological functions, including antioxidant activity and ameliorating hypertriglyceridemia. However, the application of DHA has been limited due to poor aqueous solubility and susceptible to oxidation. Here, ovalbumin(O), myosin(M), 7S soy globulin(S), and β-lactoglobulin(β), hydrolyzed by chymotrypsin, self-assembled into micelles, respectively. Adding incremental DHA to micelles caused endogenous fluorescence quenching of O, M, S, and β micelles, implying successful incorporation of DHA into hydrophobic cores of micelles(O(DHA), M(DHA), S(DHA), and β(DHA)). The results showed that micelles provided spatial stability and improved solubility, and stability against thermal and ultraviolet(UV)light for DHA. The uptake of DHA from M(DHA), β(DHA), O(DHA), and S(DHA)was 3.27-, 3.91-, 2.7-, and 3.95-fold higher, respectively, than that of DHA by Caco-2 cells. Encapsulation in micelles increased DHA aqueous solubility and uptake, which enhanced cellular endogenous antioxidant defense. Meanwhile, increased uptake of DHA was verified by HepG2 cells, and O, M, S, and β micelles were proven to increase DHA uptake to reduce lipid deposition. Our findings strongly support the possibility that O, M, S, and β micelles can be regarded as a carrier for loading DHA.

关键词： docosahexaenoic acid Micelle Solubility Stability Cellular uptake

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Protective effects of docosahexaenoic acid against non-alcoholic hepatic steatosis through activating of JAK2/STAT3 signaling pathway

引用

BIOCELL 2021年第2期45卷 307-316页

作者： YUE WANG YINPING DUAN KUNLIN CHEN HUIXIA LI YAN QUAN College of Animal Science and Technology Nanjing Agricultural UniversityNanjing210095China 2 Nanjing XinRuize Agricultural Biotechnology Co. Ltd.Nanjing210095China Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base Ministry of Science and TechnologyJiangsu Academy of Agricultural SciencesNanjing210014China College of Pharmacy Shaanxi University of Chinese MedicineXianyang712046China

Non-alcoholic fatty liver disease is the most common cause of hepatic *** the present study,human normal hepatocyte L02 cells were treated with 50%fetal bovine serum to induce the formation of hepatic steatosis in vitro,and then the cells were treated with docosahexaenoic acid to investigate its protective effect on Non-alcoholic fatty liver *** results showed that 50%of fetal bovine serum significantly induced intracellular lipid accumulation and hepatocyte fatty degeneration within 48 *** expression level of adipose formation-related genes was significantly up-regulated,such as PPARγ,C/EBPαand SREBP-1;meanwhile,the content of cellular total lipid,total cholesterol and triglycerides were significantly increased after 50%fetal bovine serum ***,docosahexaenoic acid treatment could inhibit FBS-induced intracellular lipid accumulation in L02 cells and the expression of lipogenic ***,docosahexaenoic acid treatment could reduce hepatic steatosis-induced oxidative stress and endoplasmic reticulum stress response,and these responses were shown by the modification of antioxidant enzyme activities and GRP78,CHOP *** addition,the results showed that docosahexaenoic acid can activate the JAK2/STAT3 signaling pathway in fatty liver L02 cell;inhibition of JAK2/STAT3 signaling pathway by WP1066 abolished the beneficial effects of docosahexaenoic acid on hepatic steatosis accompanied with the increased expression of lipogenic genes and endoplasmic reticulum stress *** all,the present study showed that docosahexaenoic acid can alleviate non-alcoholic hepatic steatosis by activating JAK2/STAT3 signaling pathway.

关键词： docosahexaenoic acid NAFLD L02 cell Lipid accumulation JAK2/STAT3

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Fortification of pork loins with docosahexaenoic acid(DHA) and its effect on flavour

引用

Journal of Animal Science and Biotechnology 2014年第1期5卷 106-113页

作者： William J Meadus Tyler D Turner Michael ER Dugan Jennifer L Aalhus Pascale Duff David Rolland Bethany Uttaro Lorna L Gibson Agriculture and Agri-Food Canada Lacombe Research Centre 6000 C&E TrailLacombe T4L 1 W1AB Canada

Pork is traditionally low in docosahexanoic acid （DHA, C22：6n-3） and deficient in omega-3 fats for a balanced human diet. DHA as triglycerides was commercially prepared from the microalgae Schizochytfium and injected into fresh pork loins. Treatments of a mixed brine control （CON）, 3.1% sunflower oil in mixed brine （SF） and a 3.1% DHA oil in mixed brine （DHA） were injected into pork loins at 10 mL/100 g and grilled at 205℃. After cooking, the CON and SF pork loins contained 0.03 to 0.05 mg DHA/g of pork and the DHA injected loins contained approximately 1.46 mg DHA/g. This also changed the fatty acid profile of omega-6：omega-3 from, 5 to 1 in the CON pork, to a ratio of 1.7 to 1 in DHA pork. The appearance, odor, oxidation rates and sensory taste, as judged by a trained panel, determined the DHA injected meat to be, ＇slightly desirable＇ and gave lower ＇off flavour＇ scores, relative to the CON and SF injected pork. Pork can be fortified with DHA oil to 146 mg/100 g serving, which would meet half the recommended daily omega 3 fatty acid requirements for adult humans and would be desirable in taste.

关键词： docosahexaenoic acid Injection marinade Pork Sensory characteristics

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Moderating effect of synthesized docosahexaenoic acid-enriched phosphatidylcholine on production of Th1 and Th2 cytokine in lipopolysaccharide-induced inflammation

引用

Asian Pacific Journal of Tropical Biomedicine 2018年第7期8卷 340-344页

作者： M.Choi JY.Baek I.Park SY.Lim Department of Mircrobiology Kosin University College of Medicine Division of Marine Bioscience Korea Maritime and Ocean University

Objective: To evaluate effects of docosahexaenoic acid-enriched phosphatidylcholine(DHAPC) on cytokine production induced by lipopolysaccharide(LPS). Methods: The culture supernatants of splenocytes exposed to DHA-PC along with LPS were harvested to determine the production of Th 1(IFN-kines. Cytokines were m毭 and IL-2) and Th2 [IL-4, IL-5, IL-6 and IL-12/IL-23(p40)] cytoeasured using ELISA. Results: Co-administration of DHAPC with LPS resulted in significantly lower IL-2 expression compared to that observed with administration of only LPS(P<0.01). Treatment with DHA-PC and LPS significantly increased IL-5 expression(P<0.01). Moreover, co-administration of DHA-PC with LPS significantly decreased IL-6 and IL-12/IL-23(p40) expressions compared to that observed with administration of only LPS(P<0.01). Conclusions: Our results suggest that DHA-PC inhibits pro-inflammatory cytokines [IL-2, IL-6 and IL-12/IL-23(p40)] expression on induction of inflammation.

关键词： docosahexaenoic acid Phosphatidylcholine Cytokines Interleukin-2 Interleukin-6 Interleukin-12/IL-23(p40)

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Pilot trial evaluating maternal docosahexaenoic acid consumption during pregnancy: Decreased postpartum depressive symptomatology

引用

International Journal of Nursing Sciences 2014年第4期1卷 339-345页

作者： Michelle P.Judge Cheryl T.Beck Holiday Durham Michele M.McKelvey Carol J.Lammi-Keefe University of Connecticut School of NursingStorrsCT 06269-4026USA Pennington Biomedical Research Center Baton RougeLA 70808USA Louisiana State University School of Nutrition and Food SciencesAgricultural CenterBaton RougeLA 70808USA

Objective:docosahexaenoic acid(DHA,22:6,n-3)is a major structural component of neural tissue critical to neurotransmission and mood *** maternal dietary intake coupled with accelerated maternal-fetal transfer of DHA compound risk for maternal *** objective of this investigation was to determine if maternal DHA supplementation is efficacious in reducing symptoms of postpartum ***:This pilot investigation was a randomized,double-blinded,placebo controlled investigation of the role of DHA in preventing risk for postpartum *** were assigned to:i)Placebo(no DHA,corn oil capsule),ii)DHA(300 mg DHA,fish oil capsule).Capsules were consumed from 24 to 40 weeks gestation(1 capsule 5 days/week).Forty-two participants were recruited(n=20,intervention;n=22,placebo).Maternal DHA status and depressive symptoms were followed from 24 to 40 weeks gestation using the Center for Epidemiologic Studies Depression Scale(CES-D)and the Postpartum Depression Screening Scale(PDSS)from 2 weeks to 6 months ***:PDSS total scores were significantly lower(p=0.016;46.03±2.17,intervention vs.52.11±2.4,placebo)in the intervention group with less anxiety/insecurity(p=0.03),emotional lability(p=0.04)and loss of self(p=0.02).Conclusions:Women in the DHA intervention group had fewer symptoms of postpartum depression compared to the placebo *** results support the notion that the consumption of DHA by pregnant women can be efficacious in preventing depressive symptoms and highlight a need for further larger-scale investigations using the PDSS in tandem with a diagnostic evaluation.

关键词： docosahexaenoic acid Postpartum depression n-3 polyunsaturated fatty acids Depression Pregnancy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：