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Effect of dl-praeruptorin a on desmin and vimentin content in rat ischemia/reperfusion myocardiocytes

Chinese Medical Journal 2007年第24期120卷 2256-2259页

作者： CHANG He CHU Xiao-yu ZOU Jun CHANG Tian-hui Division of Cardiology Graduate School of Medical and DentalSciences Niigata University 1-757 Asahimachi-dori Niigata951-8510 Japan Department of Clinical Pharmacology School of PharmaceuticalSciences China Medical University Shengyang 110001 China Department of Pharmacy School of Medical Sciences Xiamen University Xiamen 361005 China

Background Intermediate filament （IF） proteins have been thought to play a role in nuclear centration, organelle movement and maintenance of cell shape, dl-praeruptorin a （Pd-la）, a novel Ca^2＋-influx blocker and K^＋-channel opener isolated from Chinese traditional herbal medicine Qian-Hu, has been demonstrated to inhibit expression of apoptosis related proteins and reduce the level of proinflammatory factors in ischemia/reperfusion myocardiocytes. Morphologically, whether Pd-la effects myocardiocyte IFs remains unclear. The purpose of this study was, for the first time, to evaluate the in vivo effects of Pd-la on IF desmin and vimentin content in order to further explore its cardioprotection against ischemia and elucidate its mechanism of action. Methods Rats underwent a 30 minutes coronary occlusion followed by 120 minutes reperfusion. Assessment of desmin and vimentin content of myocardiocytes was performed by immunohistochemistry, Western blot, Hematoxylin-Eosin staining and densitometry. Results Pretreatment with i.v. infusion of Pd-la prior to ischemia significantly increased desmin and vimentin content and alleviated defects caused by the ischemia/reperfusion insult, e.g. with Pd-la at a dose of 0.5 or 1.0 mg/kg, integrated density values of desmin were increased from 61 478 ± 10 074 to 177 408 ±10 395 and 195 784±20 057, and vimentin from 59 189± 19 853 to 164 781± 19 543 and 185 696± 20 957 （P〈0.01, vs placebo）, respectively. The recovery of desmin seemed to be stronger and appeared earlier than that of vimentin. Conclusion Pd-la protectively increased IF desmin and vimentin content in ischemia/reperfusion myocardiocytes, which might be partially responsible for its cardioprotection against ischemia.

关键词： dl-praeruptorin a desmin vimentin intermediate filament ischemia myocardiocyte

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