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dihydromyricetin Alleviates H9C2 Cell Apoptosis and Autophagy by Regulating CircHIPK3 Expression and PI3K/AKT/mTOR Pathway

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Chinese Journal of Integrative Medicine 2023年第5期29卷 434-440页

作者： ZHANG Zhi-ying LIU Chao WANG Peng-xiang HAN Yi-wei ZHANG Yi-wen HAO Mei-li SONG Zi-xu ZHANG Xiao-ying Key Laboratory for Molecular Genetic Mechanisms and Intervention Research on High Altitude Disease of Tibet Autonomous Region School of MedicineXizang Minzu UniversityXianyangShaanxi Province(712082)China Key Laboratory of High Altitude Environment and Gene Related to Disease of Tibet Ministry of Education School of MedicineXizang Minzu UniversityXianyangShaanxi Province(712082)China School of Finance Economics Xizang Minzu UniversityXianyangShaanxi Province(712082)China School of Information Engineering Xizang Minzu UniversityXianyangShaanxi Province(712082)China

Objective: To investigate the effect and potential mechanism of dihydromyricetin(Dmy) on H9C2 cell proliferation, apoptosis, and autophagy. Methods: H9C2 cells were randomly divided into 7 groups, namely control, model, EV(empty p CDH-CMV-MCS-EF1-Cop GFP-T2A-Puro vector), IV(circHIPK3 interference), Dmy(50 μmol/L), Dmy+IV, and Dmy+EV groups. Cell proliferation and apoptosis were detected by cell counting kit-8 assay and flow cytometry, respectivley. Western blot was used to evaluate the levels of light chain 3 Ⅱ/Ⅰ(LC3Ⅱ/Ⅰ), phospho-phosphoinositide 3-kinase(p-PI3K), protein kinase B(p-AKT), and phospho-mammalian target of rapamycin(p-mTOR). The level of circHIPK3 was determined using reverse transcriptase polymerase chain reaction. Electron microscopy was used to observe autophagosomes in H9C2 cells. Results: Compared to H9C2 cells, the expression of circHIPK in H9C2 hypoxia model cells increased significantly(P<0.05). Compared to the control group, the cell apoptosis and autophagosomes increased, cell proliferation rate decreased significantly, and the expression of LC3Ⅱ/Ⅰ significantly increased(all P<0.05). Compared to the model group, the rate of apoptosis and autophagosomes in IV, Dmy, and Dmy+IV group decreased, the cell proliferation rate increased, and the expression of LC3Ⅱ/Ⅰ decreased significantly(all P<0.05). Compared to the control group, the expressions of p-PI3K, p-AKT, and p-mTOR in the model group significantly reduced(P<0.05), whereas after treatment with Dmy and sh-circHIPK3, the above situation was reversed(P<0.05). Conclusion: Dmy plays a protective role in H9C2 cells by inhibiting circHIPK expression and cell apoptosis and autophagy, and the mechanism may be related to PI3K/AKT/mTOR pathway.

关键词： dihydromyricetin circHIPK autophagy cell apoptosis PI3K/AKT/mTOR

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dihydromyricetin‑Incorporated Multilayer Nanofibers Accelerate Chronic Wound Healing by Remodeling the Harsh Wound Microenvironment

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Advanced Fiber Materials 2022年第6期4卷 1556-1571页

作者： Zijian Wang Wenjie You Wang Wang Weiqun Tian Fangjin Chen Yu Xiao Yun Chen Xinghuan Wang Department of Urology Zhongnan Hospital of Wuhan University430071 WuhanChina Department of Biomedical Engineering and Hubei Province Key Laboratory of Allergy and Immune Related Disease School of Basic Medicine SciencesTaiKang Medical SchoolWuhan University430071 WuhanChina Center for Quantitative Biology School of Life SciencesPeking University100871 BeijingChina

The harsh microenvironment in wound(HMW)remains a major obstacle to chronic wound healing.Although a series of bioactive materials have been developed,few of them are multi-functional and able to accelerate wound healing via precisely remodeling the HMW.Herein,a series of dihydromyricetin(DHM)-incorporated multilayer nanofibers(termed DQHP-n,n=0,2,6 and 10)are fabricated using a layer-by-layer(LBL)self-assembly technique.The average diameters of DQHP-n significantly increase from 0.30±0.16μm to 0.84±0.28μm(P<0.05)along with the n value increased from 0 to 10,the tensile strength of that is also significantly improved from 1.12±0.15 MPa to 2.16±0.30 MPa(P<0.05),and the water contact angle of that significantly decreases from 129.1±1.5°to 76.6±3.9°(P<0.05).The DQHP-n are found to be biocompatible,in which DQHP-6 promoted cell migration through activation of the epithelial–mesenchymal transformation(EMT)pathway and reconstruction of the HMW by stopping bleeding,killing bacteria,eliminating inflammation,and scavenging reactive oxygen species(ROS).The in vivo evaluation is carried out via an E.coli-infected rat skin regeneration model.The DQHP-6 group demonstrates the best effect,as it healed up to 98.5±1.0%of the wound area at day 15.DQHP-6 differentially regulates the mRNA expressions of several cytokines(FGF2,PDGF,IL-1α,IL-6,IL10,and TGF-β),which ends to reductions of total inflammatory cells(CD45^(+) cells)and M1 macrophages(CD80^(+) and CD86^(+) cells),proliferation of host cell(Ki67^(+) cells),and enhancement of collagen synthesis.In conclusion,DQHP-6 exhibits multifunctional properties for HMW,and can serve as a promising wound dressing for clinical transformation.

关键词： dihydromyricetin Quaternized chitosan Hyaluronic acid Nanofiber Wound dressing

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dihydromyricetin promotes apoptosis, suppresses proliferation and tumor necrosis factor-α-mediated nuclear factor kappa-B activation in nasopharyngeal carcinoma CNE-2 cells

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Journal of Traditional Chinese Medicine 2021年第3期41卷 367-375页

作者： LI Caihong SHI Jinlian HUANG Bo DING Hang LIN Hanguang ZENG Jincheng ZHAO Yang LUO Guoqing Institute of Biochemistry and Molecular Biology Guangdong Medical UniversityZhanjiang 524023China Dongguan Key Laboratory of Medical Bioactive Molecular Developmental and Translational Research Guangdong Provincial Key Laboratory of Medical Molecular DiagnosticsGuangdong Medical UniversityDongguan 523808China Department of Otolaryngology of Shenzhen Longhua New District Central Hospital Shenzhen 518109China Department of Otolaryngology of The Third People's Hospital of Longgang Shenzhen 518115China Faculty of Forensic Medicine of Guangdong Medical University Dongguan 523808China Department of Otolaryngology of the Second Affiliated Hospital of Guangdong Medical University Zhanjiang 524023China Department of Otolaryngology of the Affiliated Hospital of Guangdong Medical University Zhanjiang 524023China

OBJECTIVE: To investigate the efficacy of dihydromyricetin(DMY) on nasopharyngeal carcinoma(NPC) cell proliferation, apoptosis and to reveal the underlying mechanism in vitro experiments.METHODS: The CNE-2 cell line was treated with different concentrations of DMY and the effects of DMY on cell viability and proliferation were evaluated using cell counting kit-8(CCK-8) assay and plate colony formation assay. Cellular apoptosis was detected by flow cytometry following Annexin V fluorescein isothiocyanate/propidine iodide staining.Nuclei morphology was observed under a fluorescence microscope following Hoechst 333258 staining. The expression of phosphorylated inhibitor of nuclear factor kappa-B kinase subunit beta(p-IKKβ), phosphorylated inhibitor of nuclear factor kappa-B kinase subunit alpha(p-IKKα), inhibitor of nuclear factor kappa-B alpha(IκB-α), nuclear factor kappa-B(NF-κB)/p65 was examined by Western blot analysis and the nuclear translocation of NF-κB/p65 was observed using a confocal laser scanning microscopy.RESULTS: DMY inhibited the proliferative capability and colony formation of NPC CNE-2 cells. Meanwhile, DMY induced apoptosis of CNE-2 cells in a dose and time-dependent manner via upregulating B-cell lymphoma-2 associated X, but downregulating B-cell lymphoma-2 and pro-caspase-3. Importantly, we found that DMY suppressed tumor necrosis factor alpha(TNF-α)-mediated NF-κB activation via inhibiting p-IKKβ, p-IKKα and blocking NF-κB subunit p65.CONCLUSION: Our experiments demonstrated that DMY had significant antiproliferative and apoptosisinducing effects on CNE-2 cells. Additionally, DMY promoted inactivation of p-IKKβ, p-IKKα,and blocked the nuclear translocation of NF-κB subunit p65. These results suggest that DMY may be an important therapeutic approach for NPC.

关键词： dihydromyricetin Nasopharyngeal carcinoma Cell proliferation Apoptosis I-kappa B kinase NF-kappa B Signal transduction

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dihydromyricetin-mediated inhibition of the Notch1 pathway induces apoptosis in QGY7701 and Hep G2 hepatoma cells

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World Journal of Gastroenterology 2017年第34期23卷 6242-6251页

作者： Cai-Jie Lu Yi-Feng He Wei-Zhuang Yuan Li-Jun Xiang Jian Zhang Yan-Rui Liang Juan Duan Yun-He He Ming-Yi Li Laboratory of Hepatobiliary Surgery Affiliated Hospital of Guangdong Medical University the First Clinical Medical College Southern Medical University Department of Gastrointestinal Surgery. The Second Affiliated Hospital of Guangzhou Medical University TCM-Integrated Hospital Southern Medical University

AIM To investigate whether dihydromyricetin(DHM) inhibits cell proliferation and promotes apoptosis by downregulating Notch1 expression.METHODS The correlation between Notch1 and Hes1(a Notch1 target molecule) expression in hepatoma samples was confirmed by q RT-PCR. In addition, MTT assays, flow cytometry and TUNEL analysis showed that DHM possessed strong anti-tumor properties, evidenced not only by reduced cell proliferation but also by enhanced apoptosis in QGY7701 and Hep G2 hepatocellular carcinoma(HCC) cells. The expressions of Notch1, Hes1, Bcl-2 and Bax were determined by Western blot.RESULTS Among the tested samples(n = 64), the expression levels of Notch1(75% of patients) and Hes1(79.7% of patients) m RNA in tumor tissues were higher than in the normal liver tissues. There was a negative correlation between the expression of Notch1 and the degree of differentiation and positively correlated with the Alpha Fetal Protein concentration. The viability of HCC cells treated with DHM was significantly inhibited in a dose and time-dependent manner. Apoptosis was induced in Hep G2 and QGY7701 cell lines following 24 h of DHM treatment. After treatment with DHM, the protein expression of Notch1 was downregulated, the apoptosis-related protein Bax was upregulated and Bcl2 was downregulated. Notch1 si RNA further enhanced the anti-tumor properties of DHM. CONCLUSION Notch1 is involved in the development of HCC and DHM inhibits cell proliferation and promotes apoptosis by down-regulating the expression of Notch1.

关键词： dihydromyricetin Apoptosis Hepatocellular 癌 Notch1

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dihydromyricetin ameliorates chronic liver injury by reducing pyroptosis

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World Journal of Gastroenterology 2020年第41期26卷 6346-6360页

作者： Quan-Cheng Cheng Jing Fan Xin-Wei Deng Huai-Cun Liu Hui-Ru Ding Xuan Fang Jian-Wei Wang Chun-Hua Chen Wei-Guang Zhang Department of Anatomy Histology and EmbryologySchool of Basic Medical SciencesPeking University Health Science CenterBeijing 100191China Xin Hua Hospital Shanghai Jiaotong University School of MedicineShanghai 202155China

BACKGROUND Chronic liver injury(CLI) is now a worldwide disease. However, there is no effective treatment. Pyroptosis plays an essential role in CLI. dihydromyricetin(DHM) resists oxidation and protects the liver. We hypothesize that the beneficial effect of DHM on CLI is related to its effect on the expression of pyroptosisrelated molecules. Therefore, we studied the influence of DHM on CLI and pyroptosis.AIM To study the role of pyroptosis in the pathogenesis of CLI and the therapeutic mechanism of DHM.METHODS Thirty-two mice were randomly divided into four groups: The control group was injected with olive oil, the carbon tetrachloride(CCl4) group was injected with CCl4, the vehicle group was injected with hydroxypropyl-β-cyclodextrin while injecting CCl4 and the DHM group was injected with DHM while injecting CCl4. After four weeks of treatment, liver tissues from the mice were stained with hematoxylin and eosin, and oil red O. Blood was collected from the angular vein for serological analysis. The severity of CLI was estimated. Some liver tissue was sampled for immunohistochemistry, Western blotting and quantitative reverse transcription PCR to observe the changes in pyroptosis-related molecules.RESULTS Serum total cholesterol, low density lipoprotein, aspartate aminotransferase(AST) and alanine aminotransferase(ALT) in the CCl4 group were higher than those in the control group, and serum total cholesterol, low density lipoprotein, AST and ALT in the DHM group were lower than those in the vehicle group. Hematoxylin and eosin and oil red O staining showed that there were more lipid droplets in the CCl4 group than in the control group, and there were fewer lipid droplets in the DHM group than in the vehicle group. Western blotting showed that the expression of the pyroptosis-related molecules caspase-1, NOD-, LRR-and pyrin domain-containing 3(NLRP3) and gasdermin D(GSDMD)-N in the CCl4 group was higher than that in the control group, while expression of these proteins in the DHM group was lower than that in the

关键词： Chronic liver injury dihydromyricetin Pyroptosis Carbon tetrachloride Pathogenesis Steatosis

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Recent advances in research on vine tea,a potential and functional herbal tea with dihydromyricetin and myricetin as major bioactive compounds

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Journal of Pharmaceutical Analysis 2021年第5期11卷 555-563页

作者： Qili Zhang Yanfang Zhao Meiyan Zhang Yalu Zhang Hongfang Ji Liang Shen College of Life Sciences Shandong University of TechnologyZiboShandong 255000China Department of Pharmacy The First Hospital of Hebei Medical UniversityShijiazhuangHebei 050000China Department of Pharmacy The Affiliated Hospital of Jining Medical CollegeJiningShandong 272100China

Vine tea has been used as an herbal tea by several ethnic minorities for hundreds of years in China.Flavonoids,a kind of indispensable component in a variety of nutraceutical,pharmaceutical and cosmetic applications,are identified to be the major metabolites and bioactive ingredients in vine tea.Interestingly,vine tea exhibits a wide range of significant bioactivities including anti-oxidant,anti-inflammatory,anti-tumor,antidiabetic,neuroprotective and other activities,but no toxicity.These bioactivities,to some extent,enrich the understanding about the role of vine tea in disease prevention and therapy.The health benefits of vine tea,particularly dihydromyricetin and myricetin,are widely investigated.However,there is currently no comprehensive review available on vine tea.Therefore,this report summarizes the most recent studies investigating bioactive constituents,pharmacological effects and possible mechanisms of vine tea,which will provide a better understanding about the health benefits and preclinical assessment of novel application of vine tea.

关键词： Vine tea Flavonoids dihydromyricetin Myricetin Pharmacological effects Molecular mechanism

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dihydromyricetin inhibits migration and invasion of hepatoma cells through regulation of MMP-9 expression

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World Journal of Gastroenterology 2014年第29期20卷 10082-10093页

作者： Qing-Yu Zhang Ran Li Guo-Fang Zeng Bin Liu Jie Liu Yang Shu Zhong-Kao Liu Zhi-Dong Qiu Dong-Jun Wang Hui-Lai Miao Ming-Yi Li Run-Zhi Zhu Key Laboratory of Hepatic Disease Affiliated Hospital of Guangdong Medical College Department of General Surgery Jiamusi University

AIM:To investigate the effects of dihydromyricetin(DHM)on the migration and invasion of human hepatic cancer cells.METHODS:The hepatoma cell lines SK-Hep-1 and MHCC97L were used in this study.The cells were cultured in RPIM-1640 medium supplemented with 10%fetal bovine serum at 37℃in a humidified 5%CO2incubator.DHM was dissolved in dimethyl sulfoxide and diluted to various concentrations in medium before applying to cells.MTT assays were performed to measure the viability of the cells after DHM treatment.Wound healing and Boyden transwell assays were used to assess cancer cell motility.The invasive capacity of cancer cells was measured using Matrigel-coated transwell chambers.Matrix metalloproteinase(MMP)-2/9 activity was examined by fluorescence analysis.Western blot was carried out to analyze the expression of MMP-2,MMP-9,p-38,JNK,ERK1/2 and PKC-δproteins.All data were analyzed by Student’s t tests in GraphPad prism 5.0software and are presented as mean±SD.RESULTS:DHM was found to strongly inhibit the migration of the hepatoma cell lines SK-Hep-1(without DHM,24 h:120±8μmol/L vs 100μmol/L DHM,24h:65±10μmol/L,P<0.001)and MHCC97L(without DHM,24 h:126±7μmol/L vs 100μmol/L DHM,24h:74±6μmol/L,P<0.001).The invasive capacity of the cells was reduced by DHM treatment(SK-Hep-1cells without DHM,24 h:67±4μmol/L vs 100μmol/L DHM,24 h:9±3μmol/L,P<0.001;MHCC97L cells without DHM,24 h:117±8μmol/L vs 100μmol/L DHM,24 h:45±2μmol/L,P<0.001).MMP2/9 activity was also inhibited by DHM exposure(SK-Hep-1 cells without DHM,24 h:600±26μmol/L vs 100μmol/L DHM,24 h:100±6μmol/L,P<0.001;MHCC97L cells without DHM,24 h:504±32μmol/L vs 100μmol/L DHM 24 h:156±10μmol/L,P<0.001).Western blot analysis showed that DHM decreased the expression level of MMP-9 but had little effect on MMP-2.Further investigation indicated that DHM markedly reduced the phosphorylation levels of p38,ERK1/2 and JNK in a concentration-dependent manner but had no impact on the total protein levels.In addition,PKC-δprotein,a key protein in the regulation of MMP family prote

关键词： dihydromyricetin Migration Invasion Hepatic cancer

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dihydromyricetin alleviates carbon tetrachloride-induced acute liver injury via JNK-dependent mechanism in mice

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World Journal of Gastroenterology 2015年第18期21卷 5473-5481页

作者： Jun Xie Jie Liu Tu-Ming Chen Qing Lan Qing-Yu Zhang Bin Liu Dong Dai Wei-Dong Zhang Li-Ping Hu Run-Zhi Zhu Clinical Research Center Xuyi People’s Hospital Department of Hepatobiliary Surgery of Affiliated Hospital of Guangdong Medical College Zhanjiang Key Laboratory of Hepatobiliary Diseases Department of Stomatology Affiliated Hospital of Guangdong Medical College

AIM: To assess the effects of dihydromyricetin(DHM) as a hepatoprotective candidate in reducing hepatic injury and accelerating hepatocyte proliferation after carbon tetrachloride(CCl4) treatment.METHODS: C57 BL/6 mice were used in this study. Mice were orally administered with DHM(150 mg/kg) for 4 d after CCl4 treatment. Serum and liver tissue samples were collected on days 1, 2, 3, 5 and 7 after CCl4 treatment. The anti-inflammatory effect of DHM was assessed directly by hepatic histology detection and indirectly by serum levels of aspartate aminotransferase(AST), alanine aminotransferase(ALT), albumin, and superoxide dismutase(SOD). Inflammatory cytokines, such as interleukin(IL)-1β, IL-6 and tumor necrosis factor-α(TNF-α), were detected using ELISA kits. Proliferating cell nuclear antigen(PCNA) staining was used to evaluate the role of DHM in promoting hepatocyte proliferation. Hepatocyte apoptosis wasmeasured by TUNEL assay.Furthermore,apoptosis proteins Caspases-3,6,8,and 9 were detected by Western blot.SP600125 were used to confirm whether DHM regulated liver regeneration through JNK/TNF-αpathways.RESULTS:DHM showed a strong anti-inflammatory effect on CCl4-induced liver injury in mice.DHM could significantly decrease serum ALT,AST,IL-1β,IL-6 and TNF-αand increase serum albumin,SOD and liver SOD compared to the control group after CCl4 treatment(P<0.05).PCNA results indicated that DHM could significantly increase the number of PCNA positive cells compared to the control(348.9±56.0 vs 107.1±31.4,P<0.01).TUNEL assay showed that DHM dramatically reduced the number of apoptotic cells after CCl4 treatment compared to the control(365.4±99.4 vs 90.5±13.8,P<0.01).Caspase activity detection showed that DHM could reduce the activities of Caspases-8,3,6 and 9 compared to the control(P<0.05).The results of Western blot showed that DHM increased the expression of JNK and decreased TNF-αexpression.However,DHM could not affect TNF-αexpression after SP600125 treatment.Furthermore,DHM could significantly improve the surviv

关键词： dihydromyricetin Liver regeneration Tumor necrosis factor-α

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USP51/PD-L1/ITGB1-deployed juxtacrine interaction plays a cell-intrinsic role in promoting chemoresistant phenotypes in non-small cell lung cancer

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Cancer Communications 2023年第7期43卷 765-787页

作者： Jianjun Li Xuechun Xiao Yang Ou Lixia Cao Min Guo Chunchun Qi Zhaoyang Wang Yuxin Liu Qiuying Shuai Hang Wang Peiqing Sun Yi Shi Guang Yang Shuang Yang Tianjin Key Laboratory of Tumor Microenvironment and Neurovascular Regulation School of MedicineNankai UniversityTianjinP.R.China Department of Pulmonary and Critical Care Medicine The First Affiliated Hospital of Soochow UniversitySuzhouJiangsuP.R.China Department of Cancer Biology Wake Forest University School of MedicineWinston-SalemNCUSA College of Pharmacy Nankai UniversityTianjinP.R.China Institute of Transplantation Medicine Nankai UniversityTianjinP.R.China

Background:Programmed death ligand 1(PD-L1)has been demonstrated to facilitate tumor progression and therapeutic resistance in an immuneindependent manner.Nevertheless,the function and underlying signaling network(s)of cancer cell-intrinsic PD-L1 action remain largely unknown.Herein,we sought to better understand how ubiquitin-specific peptidase 51(USP51)/PD-L1/integrin beta-1(ITGB1)signaling performs a cell-intrinsic role in mediating chemotherapeutic resistance in non-small cell lung cancer(NSCLC).Methods:Western blotting and flow cytometry were employed for PD-L1 detection in NSCLC cell lines.Coimmunoprecipitation and pulldown analyses,protein deubiquitination assay,tissue microarray,bioinformatic analysis and molecular biology methods were then used to determine the significance of PD-L1 in NSCLC chemoresistance and associated signaling pathways in several different cell lines,mouse models and patient tissue samples.Ubiquitin-7-amido-4-methylcoumarin(Ub-AMC)-based deubiquitinase activity,cellular thermal shift and surface plasmon resonance(SPR)analyses were performed to investigate the activity of USP51 inhibitors.Results:We provided evidence that cancer cell-intrinsic PD-L1 conferred the development of chemoresistance by directly binding to its membrane-bound receptor ITGB1 in NSCLC.At the molecular level,PD-L1/ITGB1 interaction subsequently activated the nuclear factor-kappa B(NF-κB)axis to elicit poor response to chemotherapy.We further determined USP51 as a bona fide deubiquitinase that targeted the deubiquitination and stabilization of the PD-L1 protein in chemoresistant NSCLC cells.Clinically,we found a significant direct relationship between the USP51,PD-L1 and ITGB1 contents in NSCLC patients with chemoresistant potency.The elevated USP51,PD-L1 and ITGB1 levels were strongly associated with worse patient prognosis.Of note,we identified that a flavonoid compound dihydromyricetin(DHM)acted as a potential USP51 inhibitor and rendered NSCLC cells more sensitive to chemotherapy by targeting USP51-dependent

关键词： chemosensitivity dihydromyricetin immune-independence ITGB1 non-small cell lung cancer PD-L1 USP51

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Investigation of in vitro antioxidant activity of dihydromyricetin and flavonoids rich extract from vine tea(Ampelopsis grossedentata)

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Traditional Medicine Research 2021年第1期6卷 48-62页

作者： Fu-Yan Li Shu-Mei Chi Hong-Bing Zhang Pharmaceutical Department Traditional Chinese Medicine Hospital of ZaozhuangZaozhuang 277000China

Background:Vine tea from fermented Ampelopsis grossedentata leaves has been used as a herbal tea and folk medicine in the southern region of China for hundreds of years.The aim of this investigation was to analyze the total flavonoids found in vine tea,including three bioactive flavonoids,and the total phenolic contents in the aqueous methanol extracts of 10 vine tea samples.In addition,this study also aimed to examine the antioxidant activity of dihydromyricetin and vine tea’s flavonoid-rich extract.Methods:The total flavonoids and total phenolic content assay of extracts from vine tea were performed by ultraviolet-visible spectroscopy and epoch microplate spectrophotometer,respectively.Three bioactive flavonoids were quantified simultaneously using high performance liquid chromatography.The antioxidant activity of dihydromyricetin and vine tea’s flavonoid-rich extract was evaluated in vitro using six different methods.Results:Vine tea contained a large number of flavonoids,with dihydromyricetin as its main constituent.The flavonoid-rich extract exhibited a significant scavenging effect on superoxide anion radicals,and on 3-ethylbenzthiazoline-6-sulphonic acid and 1,1-diphenyl-2-picrylhydrazyl radicals.It also possessed definite activity in lipid peroxidation inhibition,ferric reduction,and the moderation of Fe2+ion chelation ability.There was a significant negative correlation between dihydromyricetin content and antioxidant activity in the vine tea samples,including superoxide anion radical scavenging activity(P=−0.754,Pdihydromyricetin played a dominant role in the antioxidant activities of the flavonoid-rich extract.Conclusion:Vine tea’s flavonoid-rich extract could be used as a new antioxidant source to safeguard against oxidative stress.

关键词： Vine tea Ampelopsis grossedentata Flavonoids dihydromyricetin Phenols Antioxidant activity

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