检索结果-南通市图书馆

Kinetics Study on Reaction between dihydroartemisinic acid and Singlet Oxygen: An Essential Step to Photochemical Synthesis of Artemisinin

引用

Chinese Journal of Chemical Physics 2020年第2期33卷 145-150,I0002页

作者： Xian-wang Zhang Xuan Zhao Kun-hui Liu Hong-mei Su Beijing National Laboratory for Molecular Science Institute of ChemistryChinese Academy of ScienceBeijing 100190China College of Chemistry Beijing Normal UniversityBeijing 100875China University of Chinese Academy of Sciences Beijing 100049China

Artemisinin is an excellent antimalarial drug widely used in clinical ***,due to the limitation of natural source of artemisinin,the chemical synthesis of artemisinin has achieved substantial *** acid is a key precursor for the synthesis of *** reaction of dihydroartemisinic acid with singlet oxygen to form peroxide is a pivotal step in the photochemical preparation of ***,the reaction kinetics of dihydroartemisinic acid with singlet oxygen has not been investigated ***,we report the rate constants of the reaction between dihydroartemisinic acid and singlet *** directly detecting the luminescence decay kinetics of singlet oxygen at 1270 nm at room temperature,the reaction rate constants of singlet oxygen and dihydroartemisinic acid in different solvents are obtained to be 1.81×10^5(mol/L)^-1·s^-1 in CCl4,5.69×10^5(mol/L)^-1·s^-1 in CH3CN,and 3.27×10^6(mol/L)^-1·s^-1 in DMSO,*** is found that the reaction rate constants of dihydroartemisinic acid with singlet oxygen increase as polarity of the solvent increases among the three *** results provide fundamental knowledge to optimize experiment conditions of photochemical synthesis of artemisinin for improving the yields of artemisinin.

关键词： dihydroartemisinic acid Artemisinin Singlet oxygen Rate constant Transient absorption spectroscopy

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Compartmentalized Metabolic Engineering for Artemisinin Biosynthesis and Effective Malaria Treatment by Oral Delivery of Plant Cells

引用

Molecular Plant 2016年第11期9卷 1464-1477页

作者： Karan Malhotra Mayavan Subramaniyan Khushboo Rawat Md. Kalamuddin M. Irfan Qureshi Pawan Malhotra Asif Mohmmed Katrina Cornish Henry Daniell Shashi Kumar1 Metabolic Engineering Group Malaria Research Group International Centre for Genetic Engineering and Biotechnology Aruna Asaf Ali Marg New Delhi 110067 India Department of Biotechnology Jamia Millia Islamia New Delhi 110025 India Department of Horticulture and Crop Science The Ohio State University Wooster OH 44691 USA Department of Biochemistry School of Dental Medicine University of Pennsylvania Philadelphia PA 19104 USA

Artemisinin is highly effective against drug-resistant malarial parasites, which affects nearly half of the global population and kills 〉500 000 people each year. The primary cost of artemisinin is the very expensive process used to extract and purify the drug from Artemisia annua. Elimination of this apparently unnecessary step will make this potent antimalarial drug affordable to the global population living in endemic regions. Here we reported the oral delivery of a non-protein drug artemisinin biosynthesized （~0.8 mg/g dry weight） at clinically meaningful levels in tobacco by engineering two metabolic pathways targeted to three different cellular compartments （chloroplast, nucleus, and mitochondria）. The doubly transgenic lines showed a three-fold enhancement of isopentenyl pyrophosphate, and targeting AACPR, DBR2, and CYP71AV1 to chloroplasts resulted in higher expression and an efficient photo-oxidation of di- hydroartemisinic acid to artemisinin. Partially purified extracts from the leaves of transgenic tobacco plants inhibited in vitro growth progression of Plasmodium falciparum-infected red blood cells. Oral feeding of whole intact plant cells bioencapsulating the artemisinin reduced the parasitemia levels in challenged mice in comparison with commercial drug. Such novel synergistic approaches should facilitate low-cost production and delivery of artemisinin and other drugs through metabolic engineering of edible plants.

关键词： bioencapsulation oral delivery of plant material dihydroartemisinic acid isopentenyl pyrophosphate,plant transformation drug biosynthesis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：