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Portable lensless wide-field microscopy imaging platform based on digital inline holography and multi-frame pixel super-resolution

引用

Light(Science & Applications) 2015年第1期4卷 152-162页

作者： Antonio C Sobieranski Fatih Inci H Cumhur Tekin Mehmet Yuksekkaya Eros Comunello Daniel Cobra Aldo von Wangenheim Utkan Demirci Demirci Bio-Acoustic-MEMS in Medicine(BAMM)Laboratory Division of Biomedical EngineeringDivision of Renal MedicineDepartment of MedicineBrigham and Women’s HospitalHarvard Medical SchoolBostonMAUSA Demirci Bio-Acoustic-MEMS in Medicine(BAMM)Laboratory Division of Infectious DiseasesBrigham and Women’s HospitalHarvard Medical SchoolBostonMAUSA INCoD–National Brazilian Institute for digital Convergence/LAPIX Image Processing and Computer Graphics LabFederal University of Santa CatarinaBrazil Demirci Bio-Acoustic-MEMS in Medicine(BAMM)Laboratory Stanford University School of MedicineCanary Center at Stanford for Cancer Early DetectionPalo AltoCAUSA Present Address:Department of Biomedical Engineering Faculty of Engineering Bas¸kent UniversityAnkaraTurkey VisionLab Master in Applied ComputingUniversity of Itajaı´ValleyBrazil CERTI Foundation FlorianopolisBrazil

In this paper,an irregular displacement-based lensless wide-field microscopy imaging platform is presented by combining digital in-line holography and computational pixel super-resolution using multi-frame *** samples are illuminated by a nearly coherent illumination system,where the hologram shadows are projected into a complementary metal-oxide semiconductor-based imaging *** increase the resolution,a multi-frame pixel resolution approach is employed to produce a single holographic image from multiple frame observations of the scene,with small planar *** are resolved by a hybrid approach:(i)alignment of the LR images by a fast feature-based registration method,and(ii)fine adjustment of the sub-pixel information using a continuous optimization approach designed to find the global optimum *** method for phase-retrieval is applied to decode the signal and reconstruct the morphological details of the analyzed *** presented approach was evaluated with various biological samples including sperm and platelets,whose dimensions are in the order of a few *** obtained results demonstrate a spatial resolution of 1.55 μm on a field-of-view of<30 mm^(2).

关键词： digital in-line holography image registration lensless imaging point-of-care platform pixel super-resolution

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