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Altered Expression of differential genes in Thoracic Spinal Cord Involved in Experimental Cholestatic Itch Mouse Model

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Current Medical Science 2018年第4期38卷 679-683页

作者： Ming CHEN Zhi-xiao LI Qian WANG Hong-bing XIANG Department of Anesthesiology Hubei Maternal and Child Health Hospital Wuhan 430060 China Department of Anesthesiology and Pain Medicine Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

The spinal origin of cholestatic itch in experimental obstructive jaundice mouse model remains poorly understood. In this study, the jaundice model was established by bile duct ligation （BDL） in mice, and differential gene expression patterns were analyzed in the lower thoracic spinal cord involved in cholestatic pruritus after BDL operation using high-throughput RNA sequencing. At 21st day after BDL, the expression levels of ENSRNOG00000060523, ENSRNOG00000058405 and ENSRNOG00000055193 mRNA were significantly up-regulated, and those of ENSRNOG00000042197, ENSRNOG00000008478, ENSRNOG00000019607, ENSRNOG00000020647, ENSRNOG00000046289, Gemin8, Serpina3n and Trim63 mRNA were significantly down-regulated in BDL group. The RNAseq data of selected mRNAs were validated by RT-qPCR. The expression levels of ENSRNOG00000042197, ENSRNOG00000008478, ENSRNOG00000019607, ENSRNOG00000020647, ENSRNOG00000046289 and Serpina3n mRNA were significantly down-regulated in BDL group. This study suggested that cholestatic pruritus in experimental obstructive jaundice mouse model is related with in the changes of gene expression profiles in spinal cord.

关键词： cholestatic itch differential genes spinal cord high-throughput RNA sequencing

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cDNA-SCoT Analysis of differential Expressed genes in Allium tuberosum Induced by Botrytis cinerea

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Asian Agricultural Research 2020年第10期12卷 38-41页

作者： Minghui CHEN Zhilu ZHANG Yanjiao LI Pengqiang YAO Shiping CHENG Ganqing ZHAO Key Laboratory of Ecological Restoration in Hilly Area Pingdingshan UniversityPingdingshang 467000China

[Objectives]To investigate the molecular mechanism of gene differential expression in *** under Botrytis cinerea stress.[Methods]The leaf of *** was inoculated with *** to induce resistance,then leaf samples were taken at 2,4,8,12 and 24 h,respectively,after inoculation,and used for cDNA-SCoT analysis to detect the gene differential expressions.[Results]More than 800 bands with length of 100-1800 bp were obtained using 60 SCoT primers.A total of 40 differentially expressed EST sequences were screened out,and 18 non-redundant ESTs with high quality were obtained by cluster analyses of the ESTs *** results of BlastN showed that the differential genes of *** mainly related to disease defense,signal transduction,and protein metabolism.[Conclusions]Further analysis of gene function indicated that brassinosteroid biosynthesis-like proteins,NBS-LRR type disease resistance protein,jasmonic acid induced protein,abscisic stress ripening protein may be involved in the process of the incompatible interaction between the *** and ***.

关键词： Allium tuberosum Grey mould cDNA-SCoT differential genes

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