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中国植物病理学会2015年学术年会

作者：佘小漫何自福 DU Zheng-guo TANG Ya-fei LAN Guo-bin Plant Protection Research Institute Guangdong Academy of Agricultural Sciences Guangdong Provincial Key Laboratory of High Technology for Plant Protection

Bacterial wilt of eggplant caused by Ralstonia solanacearum is one of the most devastating diseases in the *** S47 A and 0802 exhibited resistant and susceptible to bacterial wilt in greenhouse and fields,*** know the resistance mechanism of eggplant against ***,using Illumina Solexa sequencing technology,transcriptomes of S47 A and 0802 were sequenced and their gene expression profile changes were characterized at 3h,6h,12 h and 24 h after infection with ***.A total of 26521 unigenes which were assigned with at least one GO term,can be categorized into 45 functional groups.A total of 15299 unigenes had a KOG classification.A total of 15452 unigenes could be annotated in KEGG *** differential expression genes were analyzed between Sm08023 and Sm08020,Sm08026 and Sm08020,Sm080212 and Sm08020,Sm080224 and Sm08020,SmS47A3 and SmS47A0,SmS47A6 and SmS47A0,SmS47A12 and SmS47A0,SmS47A24 and SmS47A0,*** results revealed that 765,1371,1384,1257 DEGs were up-regulated,and 787,2159,2824,3047 DEGs were down-regulated at 3h,6h,12 h and 24 h post inoculation in cultivar 0802,respectively,while 943,1084,1136,1194 DEGs were up-regulated,and 759,1549,2372,2777 DEGs were down-regulated at 3h,6h,12 h and 24 h post inoculation in cultivar S47 *** results will help to gain more information about the resistance mechanism of eggplant against ***.

关键词： eggplant transcriptome differential expression genes resistant cultivar susceptible cultivar

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Screen for stage-specific expression genes between tail bud stage and heartbeat beginning stage in embryogenesis of gynogenetic silver crucian carp

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Cell Research 2002年第2期12卷 133-142页

作者： YAO HUA SHI, JUN LIU, JIAN HONG XIA, JIAN FANG GUIState Key laboratory of Freshwater Ecology and Biotechnology, Wuhan Center for Developmental Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China 1State Key laboratory of Freshwater Ecology and Biotechnology Wuhan Center for Developmental Biology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China 1State Key laboratory of Freshwater Ecology and Biotechnology Wuhan Center for Developmental Biology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China 1State Key laboratory of Freshwater Ecology and Biotechnology Wuhan Center for Developmental Biology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China 1State Key laboratory of Freshwater Ecology and Biotechnology Wuhan Center for Developmental Biology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China

A systemic study was initiated to identify stage-specific expression genes in fish embryogenesis by using suppression subtractive hybridization (SSH) technique. In this study, we presented a preliminary result on screen for stage-specific expression genes between tail bud stage (TBS) and heartbeat beginning stage (HBS) in gynogenetic silver crucian carp (Carassius auratus gibelio). Two SSH plasmid libraries specific for TBS embryos and HBS embryos were constructed, and stage-specific expression genes were screened between the two stages. 1963 TBS positive clones and 2466 HBS positive clones were sampled to PCR amplification, and 1373 TBS and 1809 HBS PCR positive clones were selected to carry out dot blots. 169 TBS dot blot positive clones and 272 HBS dot blot positive clones were sequenced. Searching GenBank by using these nucleotide sequences indicated that most of the TBS dot blot positive clones could not be found homologous sequences in the database, while known genes were mainly detected from HBS d

关键词： SSH embryonic development differential expression genes gynogenesis silver crucian carp.

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Comparative Transcriptome Analyses Reveal genes Related to Spine Development in Cucumber (Cucumis sativus)

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Phyton-International Journal of Experimental Botany 2023年第4期92卷 1059-1070页

作者： Yue Chen Huan Wang Taibai Xu Peng Zhou Junsong Pan Weiwei She Weiwei Zhang Department of Plant Science and Technology Shanghai Vocational College of Agriculture and ForestryShanghai201699China School of Agriculture and Biology Shanghai Jiaotong UniversityShanghai200240China

Fruit spine is an important quality trait of *** better understand the molecular basis of cucumber spine development and function,RNA-Seq was performed to identify differentially expressed genes(DEGs)in fruit spines of different development stages,namely,8 days before anthesis(SpBA8),anthesis(SpA)and 8 days after anthesis(SpAA8).Stage-wise comparisons obtained 2,259(SpBA8 ***),4,551(SpA ***8),and 5,290(SpBA8 ***8)*** the DEGs were classified into eight expression clusters by trend *** these DEGs,in addition to the Mict,Tril,CsTTG1,CsMYB6,NS,and Tu genes that have been reported to regulate fruit spine formation,we found that the CsHDG11,CsSCL8,CsSPL8,CsZFP6 and CsZFP8 may also be involved in spine development in *** study provides a theoretical basis for further research on molecular mechanisms of spine development in cucumber.

关键词： Cucumber transcriptome spine development differential expression genes

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Identifi cation of the differential expressive tumor associated genes in rectal cancers by cDNA microarray

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World Journal of Gastroenterology 2007年第3期13卷 341-348页

作者： Xue-Qin Gao Jin-Xiang Han Zhong-Fa Xu Wei-Dong Zhang Hua-Ning Zhang Hai-Yan Huang Key Laboratory for Biotcch-Drug Ministry of Health Key Laboratory of Modem Drugs and Technology of Shandong Province Shandong Medical and Biotechnology Center Shandong Academy of Medical Sciences Jinan 250062 Shandong Province China Department of Surgery Shandong Tumor Hospital Shandong Academy of Medical Sciences Jinan 250117 Shandong Province China Department of Experimental Pathology Institute of Basic Medicine Shandong Academy of Medical Sciences Jinan 250062 Shandong Province China

AIM： To identify tumor associated genes of rectal cancer and to probe the application possibility of gene expression profiles for the classification of tumors.METHODS： Rectal cancer tissues and their paired normal mucosa were obtained from patients undergoing surgical resection of rectal cancer. Total RNA was extracted using Trizol reagents. First strand cDNA synthesis was indirectly labeled with aminoallyl-dUTP and coupled with Cy3 or Cy5 dye NHS mono-functional ester. After normalization to total spots, the genes which background subtracted intensity did not exceed 2 SD above the mean blank were excluded. The data were then sorted to obtain genes differentially expressed by ≥ 2 fold up or down in at least 5 of the 21 patients.RESULTS： In the 21 rectal cancer patients, 23 genes were up-regulated in at least 5 samples and 15 genes were down-regulated in at least 5 patients. Hierachical cluster analysis classified the patients into two groups according to the clinicopathological stage, with one group being all above stage Ⅱ and one group all below stage Ⅱ.CONCLUSION： The up-regulated genes and downregulated genes may be molecular markers of rectal cancer. The expression profiles can be used for classification of rectal cancer.

关键词： Rectal cancer Tumor associated genes cDNA microarray differential expression genes

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Identification of genes associated with nacre color in golden-lipped pearl oyster Pinctada maxima

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Marine Science Bulletin 2021年第2期23卷 73-88页

作者： ZHAO Zihan GONG Shunlian WANG Ziman LEI Chao LI Junhui ZHENG Zhe YANG Chuangye DENG Yuewen Fisheries College of Guangdong Ocean University Zhanjiang 524088Guangdong ProvinceChina Pearl Breeding and Processing Engineering Technology Research Center of Guangdong Province Zhanjiang 524088Guangdong ProvinceChina Guangdong Provincial Engineering Laboratory for Mariculture Organism Breeding Zhanjiang 524088Guangdong ProvinceChina

Pearl oyster Pinctada maxima is mainly used to produce high-valued gold and silver colored nucleated *** developed a line with golden nacre in the mantle edge by sampling breeders from the Sanya population of pearl oyster *** is found that the total carotenoid content(TCC)in the mantle tissues of the strain group(SG)was significantly higher than that of the control group(CG).differentially expressed genes between the strain and control group(a line with silver nacre in the mantle edge)were screened.A total of 28285 genes were annotated in the ***,295 up-regulated genes and 254 down-regulated genes were annotated which the strain compared with the ***-transport and metabolism genes,such as fatty acid-binding protein,cytochrome P4503A and ATP-binding cassette sub-family A were up-regulated in the *** expression levels of candidate genes in the line were higher than those in the CG,which are basically consistent with the transcriptome *** findings are helpful to understand carotenoid metabolism in golden colored nacre formation of pearl oyster ***.

关键词： Pinctada maxima carotenoids differential expression genes

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