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Application of DNA metabarcoding to characterize the diet of the moon jellyfish Aurelia coerulea polyps and ephyrae

Acta Oceanologica Sinica 2021年第8期40卷 160-167页

作者： Tingting Sun Lei Wang Jianmin Zhao Zhijun Dong Muping Coastal Environment Research Station Yantai Institute of Coastal Zone ResearchChinese Academy of SciencesYantai 264003China University of Chinese Academy of Sciences Beijing 100049China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China

dietary studies of polyps and ephyrae are important to understand the formation and magnitude of jellyfish blooms and provide important insights into the marine food ***,the diet of polyps and ephyrae in situ is largely ***,prey species of the polyps and ephyrae of the moon jellyfish Aurelia coerulea in situ were identified using high-throughput DNA sequencing *** results show that *** polyps and ephyrae consume a variety of prey *** polyps consume both planktonic and benthic prey,including hydromedusae,copepods,ciliates,polychaetes,stauromedusae,and *** ephyrae mainly feed on copepods and *** zooplankton,including Rathkea octopunctata and Sarsia tubulosa,were frequently found as part of the diet of *** polyps and *** utilization of highthroughput sequencing technique is a useful tool for studying the diet of polyps and ephyrae in the field,complementing the traditional techniques towards a better understanding of the complex role of gelatinous animals in marine ecosystems.

关键词： Aurelia coerulea in situ dietary analysis feeding high-throughput sequencing

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A comparison of fecal sampling and direct feeding observations for quantifying the diet of a frugivorous primate

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Current Zoology 2020年第4期66卷 333-343页

作者： Jaya K.MATTHEWS Amanda R.IDLEY Beth A.KApuN Cyril C.GRUETER School of Human Sciences The University of Western AustraliaCrawleyWA 6009Australia UWA Africa Research&Engagement Centre The University of Western AustraliaCrawleyWA 6009Australia Centre for Evolutionary Biology School of Biological SciencesThe University of Western AustraliaCrawleyWA 6009Australia Centre of Excellence in Biodiversity and Natural Resource Management University of RwandaHuyeRwanda School for the Environment University of MassachusettsBostonMA 02125-3393USA

Both observational and indirect evidence are widely used to determine the diets of wild *** observations are often assumed to provide the most comprehensive reflection of diet,but many wild animals are logistically challenging to *** the regular use of observa-tional and indirect methods for inferring diet in wild animals,they have rarely been compared in detail for the same study *** 12 months this study assessed the congruence of methods in estimating the diet of a montane community of eastern chimpanzees Pan troglodytes schweinfurthii in Nyungwe National Park,Rwanda using observational scan samples and macro-scopic fecal *** assessment of the number of food species consumed each month was comparable between methods,but the estimation of the composition of items in the diet differed *** notably,the fecal samples significantly underestimated the consumption of flowers,and certain fruit species,which based on direct behavioral observations were seasonally consumed at very high ***,direct observations underestimated the consumption of leaves and pith in comparison to results present in the fecal *** results suggest that combining methods where possible is most useful for accurate monitoring of dietary trends,particularly for species that experience significant seasonal shifts in their diet.

关键词： chimpanzee comparative methods dietary analysis seasonality

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