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第三届全国植物开花·衰老与采后生物学大会

作者： Yiguo Hong Research Centre for Plant RNA Signaling College of Life and Environmental S ciencesHangzhou Normal University

Virus-induced flowering（VIF） assays exploit RNA or DNA viruses to express flowering time genes such as Flowering Locus T（FT） to induce flowering in plants1,2. Such plant virus-based techniques have recently attracted widespread attention for their fundamental and applied uses in flowering physiology and in accelerating breeding in dicot crops and woody fruit-trees. However, the insight into VIF, its potential as a powerful tool for dissecting florigenic proteins as well as its establishment in monocot crops remained to be elucidated. During my presentation, I will briefly explain different forms of virus-based technology for plant functional genomics, then move onto describing the mechanism and further applications of VIF in the short-day Nicotiana tabacum cultivar Maryland Mammoth, a dicot plant3. I will also present our latest work onextending this technology for the first time to proso millet, a monocot grass and wheat, a cereal crop4. These will lead to conclude that VIF represents a simple and efficient system to identify individual amino acids that are essential for FT-mediated floral induction and to test the ability of mono-and dicotyledonous FT genes and FT fusion proteins to induce flowering. VIF is also applicable to many economically important monocotyledonous food and biofuel crops.

关键词： Virus-Induced dicot Monocot Crops

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Diversification of the RAB Guanosine Triphosphatase Family in dicots and Monocots

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Journal of Integrative Plant Biology 2007年第8期49卷 1129-1141页

作者： Jiaming Zhang Daniel R. Hill Anne W. Sylvester State Key Biotechnology Laboratory for Tropical Crops Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences Haikou 571101 China Department of Molecular Biology 3994 1000 East University Ave. University of Wyoming Laramie Wyoming 82071 USA

RAB guanosine triphosphatases （GTPases） are key regulators of vesicle trafficking and are essential to the growth and development of all eukaryotic cells. During evolution, the RAB family has expanded in different patterns to facilitate distinct cellular, developmental and physiological adaptations. Yeast has only 11 family members, whereas mammalian RABs have expanded to 18 RAB subfamilies. Plant RABs have diversified primarily by duplicating members within a single subfamily. Plant RABs are divided into eight subfamilies, corresponding to mammalian RAB1, RAB2, RAB5, RAB6, RAB7, RAB8, RAB11 and RAB18. Functional diversification of these is exemplified by the RAB1 ls, orthologs of which are partitioned into unique cell compartments in plants where they function to transport vesicles during localized tip growth. Similarly, the RAB2 family in grasses is likely involved in vesicle secretion associated with wall expansion, as determined by analysis of over-expression mutants. We propose that dicots and monocots have also diverged in their RAB profiles to accommodate unique cellular functions between the two groups. Here we present a bioinformatics analysis comparing the RAB sub-families of rice, maize and Arabidopsis. These results will guide future functional studies to test for the role of diversification of subfamilies unique to monocots compared to dicots.

关键词： dicot GTP binding protein monocot phylogenetic analysis RAB guanosine triphosphatase

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Diversification of the RAB guanosine triphosphatase family in dicots and monocots

Diversification of the RAB guanosine triphosphatase family i...

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第二届全国植物蛋白质组学学术研讨会

作者： Daniel R.Hill Anne W.Sylvester Department of Molecular Biology 3994 1000 East University Ave.University of WyomingLaramieWyoming 82071USA

RAB guanosine tdphosphatases (GTPases) are key regulators of vesicle trafficking and are essential to the growth and development of all eukaryotic *** evolution,the RAB family has expanded in different patterns to fac... 详细信息

关键词： dicot GTP binding protein monocot phylogenetic analysis RAB guanosine triphosphatase.

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