检索结果-南通市图书馆

diallyl sulfide protects against N-nitrosodiethylamine-induced liver tumorigenesis:Role of aldose reductase

World Journal of Gastroenterology 2008年第40期14卷 6145-6153页

作者： Safinaz S Ibrahim Noha N Nassar Department of Biochemistry Faculty of PharmacyCairo University Department of Pharmacology and Toxicology Faculty of PharmacyCairo University

AIM: To evaluate the protective effect of diallyl sulfide (DAS) against N-nitrosodiethylamine (NDEA)-induced liver ***: Male Wistar rats received either NDEA or NDEA together with DAS as *** energy metabolism was assessed in terms of lactate,pyruvate,lactate/pyruvate,ATP levels,lactate dehydrogenase (LDH) and glucose-6-phosphate dehydrogenase (G6PD) *** addition,membrane disintegration of the liver cells was evaluated by measuring lipid-peroxidation products,measured as malondialdehyde (MDA); nitric oxide (NO) levels; glucose-6-phosphatase (G6Pase),catalase (CAT) and superoxide dismutase (SOD) *** DNA level,glutathione-S-transferase (GST) and cytochrome c oxidase activities were used as DNA fragmentation *** reductase (AR) activity was measured as an index for cancer cells resistant to chemotherapy and histopathological examination was performed on liver sections from different ***: NDEA significantly disturbed liver functions and most of the aforementioned *** with DAS significantly restored liver functions and hepatocellular integrity; improved parameters of energy metabolism and suppressed free-radical ***: We provide evidence that DAS exerts a protective role on liver functions and tissue integrity in face of enhanced tumorigenesis caused by NDEA,as well as improving cancer-cell sensitivity to *** is mediated through combating oxidative stress of free radicals,improving the energy metabolic state of the cell,and enhancing the activity of G6Pase,GST and AR enzymes.

关键词： N-nitrosodiethylamine diallyl sulfide Liver cancer Energy metabolism Aldose reductase

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Experimental Study on Inhibitory Effects of diallyl sulfide on Growth and Invasion of Human Osteosarcoma MG-63 Cells

引用

Journal of Huazhong University of Science and Technology(Medical Sciences) 2012年第4期32卷 581-585页

作者：胡友珍陈玲易呈志杨帆陈继革 Department of Traumatic Surgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and Technology Department of Pharmacy Wuhan Puai Hospital

The inhibitory effects of diallyl sulfide(DAS) derived from allicin on in vitro and in vivo proliferation of human osteosarcoma MG-63 cells and the action mechanism,and the influence of DAS on invasive capability of MG-63 cells were investigated in order to search for the novel medicines for *** the in vitro experiment,MG-63 cells were treated with different concentrations of DSA,and the morphological changes of MG-63 cells were observed under an inverted phase *** method was used to assay the proliferation of MG-63 ***-quantitative reverse transcription polymerase chain reaction(RT-PCR) was used to detect the VEGF mRNA expression level in MG-63 *** using Transwell invasion assay,the influence of DAS on invasive ability of MG-63 cells was *** the in vivo experiment,the nude mice MG-63 cells tumor-bearing model was established,and different concentrations of DAS were injected beside the ***-one days after treatment,the mice were killed,the tumor size and tumor inhibition rate were *** microvessel density(MVD) was determined by using *** the in vitro experiment,different concentrations of DAS could obviously inhibit proliferation of MG-63 cells in a time-and concentration-dependent ***-PCR revealed that the expression levels of VEGF mRNA in DSA groups(different concentrations) were significant reduced as compared with those in control group(all P<0.05).Transwell invasion assay indicated that in 20 and 40 μg/mL DAS groups,the number of migratory cells was 91.4±8.3 and 81.8±7.4 respectively,which was significantly declined as compared with that in control group(150.4±14.7,both P<0.05).In the in vivo experiment,DAS could significantly suppress the growth of MG-63 tumor-bearing *** demonstrated that different concentrations(20 and 40 μg/mL) of DAS could significantly decrease MVD of MG-63 tumor-bearing tissue(all P<0.05).It was suggested that DAS could inhibit th

关键词： osteosarcoma diallyl sulfide growth inhibition in vitro invasion

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Modulatory effects of diallyl sulfide against testosteroneinduced oxidative stress in Swiss albino mice

引用

Asian Journal of Andrology 2006年第6期8卷 719-723页

作者： Sahdeo Prasad Neetu Kalra Yogeshwer Shukla Environmental Carcinogenesis Division Industrial Toxicology Research CentreLucknow 226001India

Aim： To investigate the protective effect of diallyl sulfide （DAS）, a constituent of garlic, against testosterone-induced oxidative stress in male Swiss albino mice. Methods： The animals were given low （250 mg/animal） and high dose （500 mg/animal） of DAS in corn oil for 7 days along with testosterone （5 mg/kg body weight, i.p.）. At the end of the study period, the prostate and the liver were dissected to determine various antioxidant enzyme levels （catalase, superoxide dismutase, glutathione reductase, glutathione-s-transferase） and lipid peroxidation. Results： In testosterone treated mice, depleted antioxidant enzyme level was accompanied with enhancement in lipid peroxidation in prostate and liver. DAS significantly restored the testosterone-induced antioxidant enzymes and lipid peroxidation in the both organs. These changes appear to be mediated by the antioxidant-enhancing effects of DAS. Conclusion： The results of the present study suggest that DAS is effective in exerting antioxidant effects by inhibiting testosterone-induced oxidative stress and might be helpful in preventing prostate cancer.

关键词： oxidative stress antioxidants lipid peroxidation diallyl sulfide testosterone

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：