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Exosomal miR-29b found in aqueous humour mediates calcium signaling in diabetic patients with cataract

International Journal of Ophthalmology(English edition) 2021年第10期14卷 1484-1491页

作者： Chao Gao Xin Liu Fan Fan Jia-Ning Yang Xi-Yue Zhou Heng-Jun Mei Xiao-Lei Lin Yi Luo First Affiliated Hospital School of MedicineShihezi UniversityShihezi 832000Xinjiang Uygur Autonomous RegionChina Eye Institute Eye and ENT HospitalCollege of MedicineFudan UniversityShanghai 200031China State Key Laboratory of Medical Neurobiology Institutes of Brain Science and Collaborative Innovation Center for Brain ScienceShanghai Medical CollegeFudan UniversityShanghai 200031China Shanghai Key Laboratory of Visual Impairment and Restoration Science and Technology Commission of Shanghai MunicipalityShanghai 200031China Key Laboratory of Myopia(Fudan University) Chinese Academy of Medical SciencesNational Health CommissionShanghai 200031China

AIM:To investigate the role of exosomal miR-29b and Ca^(2+)in regulating the function of human lens epithelial cells(HLECs).METHODS:Exosomes were isolated from human aqueous humour(AH)by ultracentrifugation,and visualized by nanopar ticle tracking and transmission electron *** miRNA sequencing was performed to identify differentially expressed miRNAs between diabetes with cataracts(DMC)group and age-related cataracts(ARC)*** was used to predict potential target of certain *** expression of CACNA1C mRNA was determined by quantitative real-time polymerase chain reaction and CACNA1C protein was determined by Western *** of Ca^(2+)in human AH and the culture supernatant of cells were detected by the calcium assay *** counting kit-8 was used to determine cell ***:Exosomes were isolated from human AH,which had a typical cup-shaped phenotype and a particle size distribution in accordance with micro extracellular *** miRNA sequencing revealed that miR-29b was significantly downregulated in DMC group compared with ***^(2+)concentration of human AH in DMC was higher than that in *** culture supernatant of cells transfected with miR-29b inhibitors had a higher concentration of Ca^(2+)than that transfected with miR-29b ***-29b reduced the viability of HLECs by upregulating CACNA1C ***:Exosomes isolated from human AH contains abundant miRNAs.A significantly expressed miR NA,miR-29b,can affect the concentration of Ca^(2+)and regulate HLEC processes by upregulating CACNA1C.

关键词： exosomes miR-29b diabetes and cataracts Ca^(2+) CACNA1C

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