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Effect of genistein on mouse blastocyst development in vitro

Acta Pharmacologica Sinica 2007年第2期28卷 238-245页

作者： Wen-hsiung CHAN~1 Hsiang-yu LU Nion-heng SHIAODepartment of Bioscience Technology and Center for Nanotechnology,Chung Yuan Christian University Chung Li 32023,Taiwan,China Department of Bioscience Technology and Center for Nanotechnology Chung Yuan Christian University Chung Li China

Aim:To examine the cytotoxic effects of genistein,an isoflavone compound,onearly postimplantation embryonic development in ***:Mouse blasto-cysts were incubated in medium with or without genistein(25 or 50 μmol/L)ordaidzein(50 μtmol/L)for 24 *** proliferation and growth was investigated bydual differential staining,apoptosis was analyzed by terminal deoxynucleotidyltransferase-mediated dUTP nick-end labeling(TUNEL)assay,and apoptotic ornecrotic cells were visualized by Annexin-V and propidium iodide(PI)*** and postimplantation development of embryos were measured by invitro development ***:TUNEL staining and Annexin-V/PI stainingshowed that genistein dose-dependently increased apoptosis in mouseblastocysts,while daidzein,another soy isoflavone,had no such *** of the blastocysts with genistein caused fewer cells than the controlgroup and this effect was primary in the inner cell *** genistein-pretreatedblastocysts showed normal levels of implantation on culture dishes in vitro,butsignificantly fewer genistein-pretreated embryos reached the later stages of em-bryonic development versus the controls,with many of the former embryos dyingat relatively early stages of *** addition,genistein treatment de-creased the development of morulas into blastocysts,and dietary genistein wasfound to induce cell apoptosis and decrease cell proliferation in an animal assaymodel of ***:Our results collectively indicate thatgenistein treatment of mouse blastocysts induces apoptosis,decreases cellnumbers,retards early postimplantation blastocyst development,and increasesearly-stage blastocyst death in vitro,while dietary genistein appears to nega-tively affect mouse embryonic development in vivo by inducing cell apoptosisand inhibiting cell *** novel findings provide important newinsights into the effect of genistein on mouse blastocysts.

关键词： genistein blastocyst development implantation

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