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Association of Varroa destructor females in multiply infested cells of the honeybee Apis mellifera

Insect Science 2019年第1期26卷 128-134页

作者： Alexis L.Beaurepaire James D.Ellis Klemens J.Krieger Robin F.A.Moritz Institut for Biologie Martin-Luther-University Halle-WittenbergHalle a.d.SaaleGermany UR 406 Abeilles et Environnement INRACentre de Recherche Provence-Alpes-Cote d'AzurAvignonFrance Entomology &Nematology Department University Florida GainesvilleFloridaUSA Bayer Animal Health GmbH LeverkusenGermany Department of Zoology and Entomology University of PretoriaPretoriaSouth Africa

The genetic diversity of Varroa destructor (Anderson &Trueman)is limited outside its natural range due to population bottlenecks and its propensity to *** light of the arms race between *** and its honeybee (Apis mellifera L.)host, any mechanism enhancing population admixture of the mite may be *** way that admixture can occur is when two genetically dissimilar mites coinvade a brood cell, with the progeny of the foundresses *** determined the relatedness of 393 pairs of *** foundresses,each pair collected from a single bee brood cell (n =five colonies).We used six microsatellites to identify the genotypes of mites coinvading a cell and calculated the frequency of pairs with different or the same *** found no deviation from random coinvasion,but the frequency of cells infested by mites with different genotypes was *** rate of recombination,coupled with a high transmission rate of mites,homogenized the allelic pool of mites within the apiary.

关键词： Apis mellifera host-parasite coevolution inbreeding avoidance mating behavior population genetics Varroa destructor

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Genes important for survival or reproduction in Varroa destructor identified by RNAi

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Insect Science 2019年第1期26卷 68-75页

作者： Zachary Y.Huang Guowu Bian Zhiyong Xi Xianbing Xie Department of Laboratory Animal Science Nanchang UniversityNanchangJiangxiChina Department of Entomology Michigan State UniversityEast LansingMIUSA Department of Microbiology and Molecular Genetics Michigan State UniversityEast LansingMI USA Ecology Evolutionary Biology and BehaviorMichigan State UniversityEast LansingMIUSA BEACON Center for the Study of Evolution in Action Michigan State UniversityEast LansingMIUSA

The Varroa mite,(Varroa destructor),is the worst threat to honey bee health *** explore the possibility of using RNA interference to control this pest, we determined the effects of knocking down various genes on Varroa mite survival and ***-stranded RNA (dsRNA)of six candidate genes (Da,Pros26S,RpL8, RpL11,RpPO and RpS13)were synthesized and each injected into Varroa mites,then mite survival and reproduction were *** of dsRNA for Da (Daughterless)and Pros26S (Gene for proteasome 26S subunit adenosine triphosphatase)caused a significant reduction in mite survival,with 3.57%±1.94% and 30.03%±11.43% mites surviving at 72 h post-inj ection (hpi),*** mites injected with green fluorescent protein (GFP)-dsRNA showed survival rates of 81.95%±5.03% and 82.36 ±2.81%,respectively. Injections of dsRNA for four other genes (RpL8,RpL11,RpPO and RpS13)did not affect survival significantly,enabling us to assess their effect on Varroa mite *** number of female offspring per mite was significantly reduced for mites injected with dsRNA of each of these four genes compared to their GFP-dsRNA *** of the target genes was verified by real-time polymerase chain reaction for two genes important for reproduction (RpL8,RpL11)and one gene important for survival (Pros26S). In conclusion,through RNA interference,we have discovered two genes important for mite survival and four genes important for mite *** genes could be explored as possible targets for the control of Varroa destructor in the future.

关键词： Apis mellijera reproduction RNAi survival Varroa destructor

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Relationship of Secreted Salivary Protein Variants to Virulence in Hessian Fly (<i>Mayetiola destructor</i>(Say))

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Advances in Entomology 2020年第1期8卷 15-33页

作者： Yan M. Crane Charles F. Crane Brandon J. Schemerhorn USDA-ARS Crop Production and Pest Control Research Unit West Lafayette IN USA Department of Entomology Purdue University West Lafayette IN USA Department of Botany and Plant Pathology Purdue University West Lafayette IN USA

Salivary proteins are the initial contact between sedentary insect pests and their host plants. It is expected that one or more salivary proteins mediate the interaction between Hessian fly and wheat, in which a feeding site is established to the benefit of the fly. A survey of 52 loci annotated as insect secreted salivary proteins was conducted in 384 individuals evenly distributed among eight biotypes of Hessian fly (B, C, D, E, GP, L, O, and vH9). Amplicons were sequenced with Illumina, and sequence reads were aligned to the reference sequences from which primers had been designed. Positions of consistent base variation (998 in all) were identified and tabulated by biotype. No varying position was associated with biotype-wide virulence to any one of wheat resistance genes H3, H5, H6, H7/H8, H9, H11, H13, and H26. The multiplate pooling strategy utilized in this study is an effective, affordable way to reveal the genotype of hundreds of individuals at tens of genetic loci.

关键词： Hessian Fly Mayetiola destructor Virulence Insect-Plant Interaction Amplicon Sequencing

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