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dental stem cell-conditioned medium for tissue regeneration: Optimization of production and storage

World Journal of Stem Cells 2022年第4期14卷 287-302页

作者： Batoul Chouaib Frédéric Cuisinier Pierre-Yves Collart-Dutilleul Laboratory Bioengineering and Nanosciences UR_UM104 University of MontpellierMontpellier 34000France

BACKGROUND Mesenchymal stem cells(MSC)effects on tissue regeneration are mainly mediated by their secreted substances(secretome),inducing their paracrine activity.This Conditioned medium(CM),including soluble factors(proteins,nucleic acids,lipids)and extracellular vesicles is emerging as a potential alternative to cell therapy.However,the manufacturing of CM suffers from variable procedures and protocols leading to varying results between studies.Besides,there is no welldefined optimized procedure targeting specific applications in regenerative medicine.AIM To focus on conditioned medium produced from dental MSC(DMSC-CM),we reviewed the current parameters and manufacturing protocols,in order to propose a standardization and optimization of these manufacturing procedures.METHODS We have selected all publications investigating the effects of dental MSC secretome in in vitro and in vivo models of tissue regeneration,in accordance with the PRISMA guidelines.RESULTS A total of 351 results were identified.And based on the inclusion criteria described above,118 unique articles were included in the systematic review.DMSC-CM production was considered at three stages:before CM recovery(cell sources for CM),during CM production(culture conditions)and after production(CM treatment).CONCLUSION No clear consensus could be recovered as evidence-based methods,but we were able to describe the most commonly used protocols:donors under 30 years of age,dental pulp stem cells and exfoliated deciduous tooth stem cells with cell passage between 1 and 5,at a confluence of 70%to 80%.CM were often collected during 48 h,and stored at-80°C.It is important to point out that the preconditioning environment had a significant impact on DMSCCM content and efficiency.

关键词： Tissue engineering Mesenchymal stem cells dental Conditioned medium Secretome Regeneration

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Digital analysis of tooth microwear,a potential application for dental microwear monitor

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Biosurface and Biotribology 2022年第1期8卷 52-57页

作者： Gang Xue Conghu Hu Jianke Du Licheng Hua Key Laboratory of Impact and Safety Engineering Ministry of EducationSchool of Mechanical Engineering and MechanicsNingbo UniversityNingboChina Key Laboratory for Intelligent Nano Materials and Devices of Ministry of Education Nanjing University of Aeronautics and AstronauticsNanjingChina

In general,tooth wear is difficult to be noticed until it leads to toothache in vivo.Developing a dynamic dental wear monitoring system to predict tooth wear in daily life is a necessity.The translation between complex surface wear morphology and corresponding digital signal source is a technical limitation to develop this kind of monitoring system.Microwear texture analysis has been widely employed in predicting diet by a palaeontologist.The main question is whether the microwear texture analysis has potential development space to develop a sensor for monitoring tooth wear.According to obtained results,the microwear texture analysis had enough sensitivity to display the surface morphology variations for different chewing foods and various angles.The corresponding sensitive digital signal of tooth microwear surface morphology makes it possible to develop a dental microwear sensor.

关键词： corresponding signal dental sensor surface morphology tooth microwear

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Effect of High Positive Acceleration(+Gz) Environment on dental Implant Osseointegration:A Preliminary Animal Study

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Biomedical and Environmental Sciences 2019年第9期32卷 687-698页

作者： ZHU Xiao Ru DENG Tian Zheng PANG Jian Liang LIU Bing KE Jie Department of Stomatology Air Force Medical Center PLA

Objective To observe the effect of high positive acceleration(+Gz) environment on dental implant osseointegration in a rabbit model and to investigate its mechanism.Methods Forty-eight New Zealand white rabbits were randomly divided into 6 groups. The rabbit’s mandibular incisors were extracted and 1 implant was placed in each socket immediately. After 1 week of rest, the rabbits were exposed to a high +Gz environment, 3 times a week. The rabbits were sacrificed at 3 weeks(2 weeks +Gz exposure), 5 weeks(4 weeks +Gz exposure), and 12 weeks(4 weeks +Gz exposure and 7 weeks normal environment) after surgery, respectively. Specimens were harvested for micro-CT scanning, histological analysis, and real-time polymerase chain reaction examination.Results Compared with those in the control group, the mRNA expression levels of bone morphogenetic protein-2(BMP-2), osteopontin(OPN), and transforming growth factor-β1(TGF-β1)were significantly lower(P < 0.05), while the mRNA expression level of receptor activator of nuclear factor κB ligand(RANKL) and the RANKL/osteoprotegerin(OPG) ratio were significantly higher(P < 0.05)at 3 weeks;values of bone volume fraction, trabecular number, bone-implant contact(BIC), and TGF-β1 and OPG mRNA expression levels were significantly lower(P < 0.05), and the value of trabecular separation, RANKL mRNA expression level and RANKL/OPG ratio were significantly higher(P < 0.05) at 5 weeks;and the value of BIC was still significantly lower(P < 0.05) at 12 weeks in the experimental group.Conclusion Early exposure to the high +Gz environment after implant surgery might have an adverse effect on osseointegration, and its mechanism could be related to the inhibition of osteoblast activity and promotion of osteoclast activity.

关键词： Positive acceleration Osseointegration dental implant Micro-CT Real-time polymerase chain reaction Osteoblast

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Neural crest derived stem cells from dental pulp and tooth-associated stem cells for peripheral nerve regeneration

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Neural Regeneration Research 2020年第3期15卷 373-381页

作者： Alessandra Pisciotta Laura Bertoni Antonio Vallarola Giulia Bertani Daniela Mecugni Gianluca Carnevale Department of Surgery MedicineDentistry and Morphological Sciences with Interest in TransplantOncology and Regenerative MedicineUniversity of Modena and Reggio EmiliaModenaItaly Department of Life Sciences University of Modena and Reggio EmiliaModenaItaly Azienda USL-Institute and Health Care(IRCCS)di Reggio Emilia Reggio EmiliaItaly

The peripheral nerve injuries,representing some of the most common types of traumatic lesions affecting the nervous system,are highly invalidating for the patients besides being a huge social burden.Although peripheral nervous system owns a higher regenerative capacity than does central nervous system,mostly depending on Schwann cells intervention in injury repair,several factors determine the extent of functional outcome after healing.Based on the injury type,different therapeutic approaches have been investigated so far.Nerve grafting and Schwann cell transplantation have represented the gold standard treatment for peripheral nerve injuries,however these approaches own limitations,such as scarce donor nerve availability and donor site morbidity.Cell based therapies might provide a suitable tool for peripheral nerve regeneration,in fact,the ability of different stem cell types to differentiate towards Schwann cells in combination with the use of different scaffolds have been widely investigated in animal models of peripheral nerve injuries in the last decade.dental pulp is a promising cell source for regenerative medicine,because of the ease of isolation procedures,stem cell proliferation and multipotency abilities,which are due to the embryological origin from neural crest.In this article we review the literature concerning the application of tooth derived stem cell populations combined with different conduits to peripheral nerve injuries animal models,highlighting their regenerative contribution exerted through either glial differentiation and neuroprotective/neurotrophic effects on the host tissue.

关键词： glial differentiation human dental pulp stem cells nerve regeneration neural crest neuroprotection tooth

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Transmission routes of 2019-nCoV and controlsin dental practice

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International Journal of Oral Science 2020年第1期12卷 25-30页

作者： Xian Peng Xin Xu Yuqing Li Lei Cheng Xuedong Zhou Biao Ren State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases&Department of Cariology and Endodontics West China Hospital of StomatologySichuan UniversityChengduChina

A novel β-coronavirus(2019-nCoV) caused severe and even fatal pneumonia explored in a seafood market of Wuhan city, Hubei province, China, and rapidly spread to other provinces of China and other countries. The 2019-nCoV was different from SARS-Co V,but shared the same host receptor the human angiotensin-converting enzyme 2(ACE2). The natural host of 2019-nCoV may be the bat Rhinolophus affinis as 2019-nCoV showed 96.2% of whole-genome identity to Bat Co V Ra TG13. The person-to-person transmission routes of 2019-nCoV included direct transmission, such as cough, sneeze, droplet inhalation transmission, and contact transmission, such as the contact with oral, nasal, and eye mucous membranes. 2019-nCoV can also be transmitted through the saliva, and the fecal–oral routes may also be a potential person-to-person transmission route. The participants in dental practice expose to tremendous risk of 2019-nCoV infection due to the face-to-face communication and the exposure to saliva, blood, and other body fluids, and the handling of sharp instruments. dental professionals play great roles in preventing the transmission of2019-nCoV. Here we recommend the infection control measures during dental practice to block the person-to-person transmission routes in dental clinics and hospitals.

关键词： dental measures sharp

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Sudden sensorineural hearing loss(SSHL) following a local anesthetic dental procedure

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Journal of Otology 2019年第2期14卷 67-72页

作者： Yi Wang Jun-Kai Cao Hui-Xin Yang Jin Feng Qi-you Zhou Fei Ji Department of Stomotology General Hospital of the PLABeijing100853China Department of Otolaryngology General Hospital of the PLABeijing100853China Key Laboratory of Hearing Impairment Science(Chinese PLA Medical School) Ministry of EducationBeijing100853China Beijing Key Laboratory of Hearing Impairment Prevention and Treatment Laboratory of Military Acoustic Trauma ProtectionBeijing100853China

Acute sensorineural hearing loss is an uncommon phenomenon in dentistry.We describe the case of a 79-year-old male who presented with acute sensorineural hearing loss occurring 2 days after a tooth extraction procedure under local anesthesia.Possible mechanisms are discussed.He was treated with vasodilators(Ginaton and Alprostadil Injection)and Mecobalamin injection with benefit.High dose oral steroids(1 mg/kg)and low molecular weight dextran were used.

关键词： Sudden sensorineural hearing loss dental procedure Acute hearing loss

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Microstructure and dry wear properties of Ti-Nb alloys for dental prostheses

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中国有色金属学会会刊：英文版 2009年第S3期19卷 639-644页

作者：徐丽娟肖树龙田竟陈玉勇黄玉东 School of Materials Science and Engineering Harbin Institute of Technology School of Chemical Engineering and Technology Harbin Institute of Technology

The microstructure and properties of a series of binary Ti-Nb alloys for dental prostheses with niobium contents ranging from 5% to 20% were investigated. The experimental results indicate that the crystal structure and morphology of Ti-Nb alloys are sensitive to their niobium contents. When Nb content is 5%, the acicular α crystal grain is observed. When Nb content is 10%, the coarse equiaxed crystal grain and the fine, acicular α crystal grain are observed. When Nb content is 15%, only the α equiaxed crystal grain is observed. When the alloy contains 20%Nb, the equiaxed and dendritic α crystal grain are observed. For Ti-Nb alloys, the increase of Nb content modifies the microstructure of Ti-Nb alloys significantly and decreases their compression elastic modulus, in which Ti-20Nb alloy shows the largest compression strength and Ti-5Nb alloy shows the best plasticity. The dry wear resistance of Ti-Nb alloys against Gr15 ball was investigated on CJS111A ball-disk wear instrument. For Ti-Nb alloys, Ti-10Nb alloy shows a smallest steady friction coefficient, Ti-5Nb alloy shows the smallest wear depth and best wear resistance, and Ti-15Nb alloy shows the largest wear depth and worst wear resistance. The phenomenon of furrow cut happens and furrows form during wear tests.

关键词： Ti-Nb alloy microstructure dry wear resistance dental prostheses material

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提高牙本质粘接耐久性的新型单体研究进展

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现代口腔医学杂志 2024年第1期38卷 45-48页

作者：魏飞孙广迪陈雪柏婷婷朱松吉林大学口腔医院修复科长春130021

龋病作为口腔常见疾病之一,目前常采用光固化复合树脂进行充填治疗。随着高分子材料和临床操作技术的不断更新与发展,牙本质的即刻粘接修复效果较为理想,但是其长期稳定性仍然存在着巨大的挑战[1]。据美国国家牙科和颅面研究协会(Nation... 详细信息

龋病作为口腔常见疾病之一,目前常采用光固化复合树脂进行充填治疗。随着高分子材料和临床操作技术的不断更新与发展,牙本质的即刻粘接修复效果较为理想,但是其长期稳定性仍然存在着巨大的挑战[1]。据美国国家牙科和颅面研究协会(National Institute of dental and Craniofacial Research,NIDCR)的统计数据显示。

关键词：光固化复合树脂充填治疗粘接修复临床操作技术牙本质长期稳定性 dental 高分子材料

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Immunomodulatory properties of dental tissue-derived mesenchymal stem cells: Implication in disease and tissue regeneration

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World Journal of Stem Cells 2019年第9期11卷 604-617页

作者： Oleh Andrukhov Christian Behm Alice Blufstein Xiaohui Rausch-Fan Division of Conservative Dentistry and Periodontology University Clinic of Dentistry Medical University of Vienna

Mesenchymal stem cells(MSCs)are considered as an attractive tool for tissue regeneration and possess a strong immunomodulatory ability.dental tissuederived MSCs can be isolated from different sources,such as the dental pulp,periodontal ligament,deciduous teeth,apical papilla,dental follicles and gingiva.According to numerous in vitro studies,the effect of dental MSCs on immune cells might depend on several factors,such as the experimental setting,MSC tissue source and type of immune cell preparation.Most studies have shown that the immunomodulatory activity of dental MSCs is strongly upregulated by activated immune cells.MSCs exert mostly immunosuppressive effects,leading to the dampening of immune cell activation.Thus,the reciprocal interaction between dental MSCs and immune cells represents an elegant mechanism that potentially contributes to tissue homeostasis and inflammatory disease progression.Although the immunomodulatory potential of dental MSCs has been extensively investigated in vitro,its role in vivo remains obscure.A few studies have reported that the MSCs isolated from inflamed dental tissues have a compromised immunomodulatory ability.Moreover,the expression of some immunomodulatory proteins is enhanced in periodontal disease and even shows some correlation with disease severity.MSC-based immunomodulation may play an essential role in the regeneration of different dental tissues.Therefore,immunomodulation-based strategies may be a very promising tool in regenerative dentistry.

关键词： Mesenchymal stem cells dental tissue Immunomodulation Peripheral blood mononuclear cells Oral diseases Tissue regeneration

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Surface and Subsurface Defects Studies of dental Alloys Exposed to Sandblasting

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Acta Metallurgica Sinica(English Letters) 2019年第10期32卷 1181-1194页

作者： Krzysztof Siemek Miroslaw Kulik Marat Eseev Miroslaw Wrobel Andrey Kobets Oleg Orlov Alexey Sidorin Joint Institute for Nuclear Research Joliot-Curie 6 Dubna Moscow Region Russian Federation 141980 Institute of Nuclear Physics PAS E. Radzikowskiego 152 31-342 Krakow Poland Institute of Physics Maria Curie-Sklodowska University pl. M. Curie-Skiodowskiej 1 20-031 Lublin Poland Northern Arctic Federal University Severnaya Dvina Emb. 17 Arkhangelsk Russian Federation 163002 AGH University of Science and Technology 30 Mickiewicza Ave 90-059 Krakow Poland Institute of Electrophysics and Radiation Technologies NAS of Ukraine Chernyshevsky St. 2& Kharkiv 61002 Ukraine

The defects created in commercial dental alloys during blasting with alumina particles propelled in compressed air under pressure 0.1 and 0.4 MPa have been studied using positron annihilation spectroscopy, scanning electron microscopy and X-ray diffraction. It was observed that higher pressure causes the increase in roughness and damaged zone range. The type of defects was determined as vacancies on dislocations. The defect concentration decreases with the depth and depends on alloys' type and applied pressure. The Rutherford backscattering spectroscopy and variable energy positron beam studies indicate shallow alumina deposition in material and show that small pressure of 0.1 MPa is not enough to remove metal surface oxides completely in 60 s in all studied dental alloys.

关键词： Sandblasting Positron annihilation technique dental alloys Defects analysis Oxides

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