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Effect of an extraction solvent on the antioxidant quality of Pinus densiflora needle extract

Journal of Pharmaceutical Analysis 2019年第3期9卷 193-200页

作者： Thamizhiniyan Venkatesan Young-Woong Choi Young-Kyoon Kim Department of Forest Products and Biotechnology College of Science and TechnologyKookmin University

Pinus densiflora needle extract (PDNE) is widely reported to have many pharmacological activities including antioxidant potential. However, the solvent system used for extraction greatly affects its antioxidant quality. Hence, in the present study, we investigated the effect of a different ratio (vol/vol) of ethanol to water (0-100%) in the extraction of PDNE with potent antioxidant capacity. The chemical assays, 2,2-diphenyl-1 picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), were conducted to assess the antioxidant potential of PDNE. Subsequently, the cytoprotective effect of PDNE was determined using tert-butyl hydroperoxide (TBHP)-challenged HepG2 cellular model. The needle extracts from 40% ethanol (PDNE-40) showed greater radical scavenging activity followed by 60%, 20%, 80%, 0% and 100% ethanol extracts. EC50 value of the most active extract, PDNE-40, was 8.56 ± 0.51 μg/mL, relative to 1.34 ± 0.28 μg/mL of the standard trolox (for ABTS radical), and 75.96 ± 11.60 μg/mL, relative to 4.83 ± 0.26 μg/mL of the standard trolox (for DPPH radical). Either PDNE-20 or PDNE-40 pretreatment remarkably decreased the levels of reactive oxygen species (ROS), lipid peroxides and protein carbonyls in TBHP-challenged HepG2 cells. In addition, both PDNE-20 and PDNE-40 significantly reversed the decreased ratio of reduced (GSH) to oxidized (GSSG) glutathione. Moreover, these two extracts showed a significant inhibitory effect on TBHP-induced nuclear damage and loss of cell viability. In summary, the inclusion of 40% ethanol in water for extraction of Pinus densiflora needle greatly increases the antioxidant quality of the extract.

关键词： Pinus densiflora needle extract Oxidative stress Cytoprotective Natural antioxidant

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Micropropagation of Pinus densiflora and the evaluation of nematode resistance of regenerated microshoots in vitro

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Journal of Forestry Research 2019年第2期30卷 519-528页

作者： Lihua Zhu Xiaofang Chu Tingyu Sun Jianren Ye Xiaoqin Wu Institute of Forest Protection College of Forest Nanjing Forestry University Co-Innovation Center for Sustainable Forestry in Southern China

To accelerate the breeding and selection of Pinus densiflora Siebold and Zucc. resistance to pine wilt disease, a micropropagation system was established and nematode resistance evaluated in vitro. Cotyledon-hypocotyl explants from 28-day-old seedlings were first cultured on Gresshoff and Doy medium supplemented with 4.0 mg L^(-1) 6-benzyladenine and 0.2 mg L^(-1) a-naphthaleneacetic acid(NAA) to stimulate the formation of buds. Induced buds were subsequently subcultured on Gupta and Durzan medium supplemented with 0.1%(w/v)activated charcoal for elongation. Stem sections derived from shoots were used as explants for the further multiplication. Roots were formed from shoots transferred to woody plant medium containing 0.2 mg L^(-1) NAA for4 weeks. The nematode resistance test showed that symptoms in micropropagated shoots after infection with pine wood nematode(PWN) were similar to those in plants infected in the field. The wilting rate varied from 20 to100% among different clones 18 days after *** most susceptible clone was Clone 6-4 with a 100%wilting rate, while Clone 8-4 showed a relatively high resistance with a 20% wilting rate. The number of nematodes recovered from Clone 8-4 shoots was significantly lower(P = 0.05) than from Clones 5-10 and 16-4. This work contributes to the breeding of PWN resistance in ***.

关键词： Pinus densiflora Micropropagation Shoot proliferation Bursaphelenchus xylophilus Nematode resistance

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Carbon and nitrogen storage in an age-sequence of Pinus densiflora stands in Korea

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Science China(Life Sciences) 2010年第7期53卷 822-830页

作者： NOH Nam-Jin SON Yowhan LEE Sue-Kyoung SEO Kyung-Won HEO Su-Jin1 YI Myong-Jong PARK Pil-Sun KIM Rae-Hyun SON Yeong-Mo LEE Kyeong-Hak Division of Environmental Science and Ecological Engineering Korea University Korea Forest Research Institute Department of Forest Resources Kangwon National University Department of Forest Sciences Seoul National University

The carbon(C) and nitrogen(N) storage capabilities of Pinus densiflora in six different stand ages(10,27,30,32,44,and 71 years old) were investigated in *** sample trees were destructively harvested and 12 were *** from the above and belowground tree components,coarse woody debris(CWD),forest floor,and mineral soil(0-30 cm) were *** biomass was highest in the 71-year-old stand(202.8 t ha-1) and lowest in the 10-year-old stand(18.4 t ha-1).C and N storage in the mineral soil was higher in the 71-year-old stand than in the other stands,mainly due to higher soil C and N ***,the total ecosystem C and N storage(tree+forest floor+CWD+soil) was positively correlated with stand age:increasing from a minimum in the 10 year old stand(18.8 t C ha-1 and 1.3 t N ha-1) to a maximum in the 71-year-old stand(201.4 t C ha-1 and 8.5 t N ha-1).The total ecosystem C storage showed a similar sigmoidal pattern to that of tree C storage as a function of the age-sequence,while N storage in the CWD,forest floor and mineral soil showed no significant temporal *** results provide important insights that will increase our understanding of C and N storage in *** stands and our ability to predict changes according to stand age in the region.

关键词： age-sequence allometric equation biomass carbon nitrogen Pinus densiflora stand age

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彰武松在北京地区的嫁接繁育效果分析

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林业科技通讯 2019年第7期 66-68页

作者：鄢鹏朱媛君张博李国雷孟鹏段树生杨晓晖吴梦瑶北京林业大学北京100083 中国林业科学研究院荒漠化研究所北京100091 北京市林业工作总站北京100029 辽宁省固沙造林研究所辽宁阜新123000

以樟子松(Pinus sylvestris ***)为砧木,从嫁接时间、接穗的切面长度及来源等方面对彰武松(Pinus densiflora ***)嫁接技术进行一系列试验。结果表明:在嫁接时间方面, 4月下旬到5月上旬嫁接可以保证较高的成活率;在接穗切面长度(L)方面,... 详细信息

以樟子松(Pinus sylvestris ***)为砧木,从嫁接时间、接穗的切面长度及来源等方面对彰武松(Pinus densiflora ***)嫁接技术进行一系列试验。结果表明:在嫁接时间方面, 4月下旬到5月上旬嫁接可以保证较高的成活率;在接穗切面长度(L)方面, 6.0≤L<8.0 cm可以保证较高的成活率;在接穗来源方面,外地接穗和本地接穗的嫁接成活率差异不显著,但考虑到运营成本的问题,选择本地接穗更为合适。研究结果可为彰武松在北京地区的繁育推广提供一定的参考依据和技术支撑。

关键词：彰武松 Pinus densiflora var.zhangwuensis 樟子松 Pinus sylvestris var.mongolica 嫁接接穗成活率

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