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Effects of organic carbon consumption on denitrifier community composition and diversity along dissolved oxygen vertical profiles in lake sediment surface

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Journal of Oceanology and Limnology 2020年第3期38卷 733-744页

作者： HONG Pei GONG Shihao WANG Chunbo SHU Yilin WU Xingqiang TIAN Cuicui Oscar Omondi DONDE CAI Pei WU Huaming XIAO Bangding Key Laboratory of Algal Biology of the Chinese Academy of Sciences Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China University of Chinese Academy of Sciences Beijing 100049China College of Life Sciences Anhui Normal UniversityWuhu 241000China

At present,the understanding of the dynamics of denitrifiers at different dissolved oxygen(DO)layers under organic carbon consumption within the surface sediments remains *** this study,high-throughput sequencing and quantitative PCR targeting nirS gene were used to analyze the denitrifier abundance dynamics,community composition,and structure for aerobic(DO 0.5-6.9 mg/L),hypoxic-anoxic(DO 0-0.5 mg/L),and anoxic(DO 0 mg/L)layers in surface sediments under organic carbon *** on the analysis of nirS gene abundance,the values of denitrifying bacteria decreased with organic carbon consumption at different DO *** the bacterial species abundance at the genus level were compared between the high-carbon and low-carbon sediments,there was significant increase in 6 out of 36,7 out of 36 and 6 out of 36 genera respectively for the aerobic,hypoxic-anoxic and anoxic *** the other hand,14 out of 36,9 out of 36 and 15 out of 36 genera showed significant decrease in bacterial species abundance respectively for the aerobic,hypoxic-anoxic and anoxic ***,14 out of 36,20 out of 36,and 15 out of 36 genera had no change in bacterial species abundance respectively for the aerobic,hypoxic-anoxic,and anoxic *** indicates that the carbon utilization ability of different denitrifiers on each DO layers was generally different from each *** of denitrifying bacteria also presented significant differences in different DO layers between the high-and low-carbon content sediment ***,under the high-carbon and low-carbon content,the abundance of nirS gene showed a high peak within the hypoxic-anoxic regions,suggesting that this region might be the main distribution area for the denitrifying bacteria within the surface ***,community of unique denitrifiers occurred in different DO layers and the adaptive changes of the denitrifier community followed the organic carbon consumption.

关键词： eutrophic freshwater lake surface sediments dissolved oxygen profi les denitrifier organic carbon consumption

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Chronic effects of different fertilization regimes on nirS-type denitrifier communities across the black soil region of Northeast China

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Pedosphere 2020年第1期30卷 73-86页

作者： Xiaojing HU Junjie LIU Dan WEI Ping ZHU Xi'an CUI Baoku ZHOU Xueli CHEN Jian JIN Xiaobing LIU Guanghua WANG Key Laboratory of Mollisols Agroecology Northeast Institute of Geography and AgroecologyChinese Academy of SciencesHarbin 150081(China) Institute of Soil and Fertilizer and Environment Resources Heilongjiang Academy of Agricultural SciencesHarbin 150086(China) Institute of Agricultural Resource and Environment Jilin Academy of Agricultural SciencesChangchun 130033(China) Heihe Branch of Heilongjiang Academy of Agricultural Sciences Heihe 164300(China) Institute of Plant Nutrition and Resources Beijing Academy of Agricultureand Forestry SciencesBeijing 100097(China)

Denitrification is one of the major processes causing nitrogen loss from arable *** study aimed to investigate the responses of nir S-type denitrifier communities to different chronic fertilization regimes across the black soil region of Northeast *** samples were collected from sites located in the north(NB),middle(MB),and south(SB)of the black soil region of Northeast China,each with four chronic fertilization regimes:no fertilizer(No F),chemical fertilizer(CF),manure(M),and chemical fertilizer plus manure(CFM).Methods of quantitative polymerase chain reaction(q PCR)and Illumina Mi Seq sequencing were applied to assess the abundance and composition of denitrifier communities by targeting the nir S *** results showed that the M and CFM regimes significantly increased the abundances of nir S-type denitrifiers compared with No F at the three *** majority of nir S sequences were grouped as unclassified denitrifiers,and the different fertilizers induced little variation in the relative abundance of known nir S-type denitrifier *** 90%of the sequences were shared among the four fertilization regimes at each location,but none of the abundant operational taxonomic units(OTUs)were shared among the three *** coordinate analysis(PCo A)revealed that the communities of nir S-type denitrifier were separated into three groups that corresponded with their *** similar fertilization regimes did not induce consistent changes in the nir S-type denitrifier communities,soil p H and NO-3-N content simultaneously and significantly influenced the structure of nir S-type denitrifier communities at the three *** results highlight that geographical separation rather than chronic fertilization was the dominant factor determining the nir S-type denitrifier community structures,and similar chronic fertilization regimes did not induce consistent shifts of nir S-type denitrifier communities in the black soils.

关键词： denitrification denitrifier diversity denitrifying gene geographical separation Illumina MiSeq sequencing manure Mollisols nirS gene quantitative polymerase chain reaction

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Isolation of aerobic denitrifier and characteristics of enhanced nitrogen removal in concentric-circles reactor with diversion wall

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Journal of Central South University 2009年第S1期16卷 374-378页

作者：许晓毅汤丽娟罗固源蒋真玉 Key Laboratory of Three Gorges Reservoir Region's Eco-Environment Ministry of EducationChongqing University Key Laboratory of Three Gorges Reservoir Region’s Eco-Environment Ministry of EducationChongqing University

Three strains of aerobic denitrifiers,named as AT3,AT6 and AT7,were isolated from concentric-circles reactor with diversion wall possessing simultaneous nitrification and denitrification (SND) effect of 69%. The three strains are all gram-positive and rod-shaped,and their colonial colors are pale yellow,milk white and pink,respectively. Combined with 16SrDNA sequence homology comparison and biochemical tests,AT3 and AT7 were identified to belong to Rhodococcus,and AT6 to Gordonia. These bacterial strains could grow well in the medium with potassium nitrate as nitrogen source and sodium citrate as carbon source. Based on the enhanced nitrogen removal experiments of selected bacteria mixture for activated sludge,the inoculum amount of 5% was supposed to be proper. The mixed biomass suspension of selected strains with PVA immobilization was put into the concentric-circles reactor in order to study the characteristics of enhanced nitrogen removal after amplifying cultivation with inoculated amount of 5%. The experimental results show that the average removal efficiencies of ammonia nitrogen (NH3-N) and total nitrogen (TN) in the reactor enhanced with aerobic denitrifying bacteria using PVA are 92.18% and 79.14% respectively,increasing by 5.29% and 7.83% respectively compared with removal effects of control group without strains enhancement.

关键词： nitrification denitrification aerobic denitrifier biological nitrogen removal polyvinyl alcohol immobilization

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Organic manure input and straw cover improved the community structure of nitrogen cycle function microorganism driven by water erosion

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International Soil and Water Conservation Research 2022年第1期10卷 129-142页

作者： Yulong Shi Qingwen Zhang Xingren Liu Xuekai Jing Chang Shi Li Zheng Agricultural Clean Watershed Resewarch Group Institute of Environment and Sustainable Development in AgricultureChinese Academy of Agricultural SciencesBeijing100081China

Water erosion process induces differences to the nitrogen(N)functional microbial community structure,which is the driving force to key N processes at soil-water ***,how the soil N trans-formations associated with water erosion is affected by microorganisms,and how the microbial respond,are still *** objective of this study is to investigate the changes of microbial diversity and community structure of the N-cycle function microorganisms as affected by water erosion under application of organic manure and straw *** the basis of iso-nitrogen substitution,four treatments were set up:1)only chemical fertilizer with N 150 kg ha^(-1),P2O560 kg ha^(-1) and K2O 90 kg ha^(-1)(CK);the N was substituted 20%by 2)organic manure(OM);3)straw(SW);and 4)organic manure+straw(1:1)(OMSW).The results showed that applying organic manure and straw to sloping farmland can increase soil N contents,but reduce runoff depth,Kw,sediment yield and N loss,especially in the *** cover and straw+organic manure increased the diversity(Chao1)of nitrifier(AOB),and both diversity and uniformity(Shannon)of denitrifier(nirK/S)were increased in the *** erosion control mea-sures reduced N-fixing bacteria diversity and increased their uniformity,and the combined application of organic manure and straw cover was a better erosion control measure than the single application of *** soil chemistry and erodibility were the main drives for the changes of N-functional microbial community structure and the appearance of dominant bacteria with different organic materials.

关键词： Water erosion Soil erodibility Organic amendment Nitrifier denitrifier N-fixing bacteria

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