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Pathogenesis of demyelinating encephalopathy in Dogs with Spontaneous Acute Canine Distemper

Journal of Integrative Agriculture 2013年第2期12卷 334-343页

作者： PAN Yao-qian LIU Xing-you MENG Li-ping ZHU Guang-rui XIA Yin-ke CHEN Jin-shan Yoshikawa Takashi Department of Veterinary Pathology College of Animal Sciences Henan Institute of Science and Technology Department of Microbiology and Immunology Northwestern University Feinberg Medical School Department of Veterinary Pathology School of Veterinary Medicine and Animal Science Kitasato University

So far, the pathogenesis of demyelination caused by canine distemper virus （CDV） in the central nervous system has remained unclear, although a lot of studies have been done extensively. To further investigate the relation of variety cells in brain to demyelination, this study was performed on 15 dogs with spontaneous acute canine distemper and 2 controls. According to anatomical relation, the brain was divided into cerebrum, cerebral stem and cerebellum. The sections with no, mild, moderate, or severe demyelinating lesions were selected respectively and stained by HE and immunohistochemistry. Immuno-localisation of CDV antigen was used to conftrm CDV infection. The brain was examined for co-localisation of the CDV antigen with either an astrocyte-specific marker, glial fibrillary acidic protein （GFAP）, or an oligodendrocyte-specific marker, galactocerebroside （GalC）. Apoptotic cell was detected by TdT-mediated nick end-labeling assay （TUNEL）. The results demonstrated that the local disturbance of blood circulation mainly included congestion, edema, thrombosis, and disseminated intravascular coagulation （DIC）. The CDV neucleocapsid protein positive reaction, metabolic disorder and apoptosis of oligodendrocytes were observed in demyelinating areas. Lots of astrocytes displayed CDV antigen-positive, especially in their process. Some of them became apoptotic cell confirmed by TUNEL staining. Fibrous astrocytes showed more intense GFAP-positive in mild and moderate demyelinating area. Some of nervous cells located in pyramidal cell layers and nucleus nervi were in degeneration, necrosis. Satellitosis, neuronophagia and apoptotic neurons were examined by hematoxylin and eosin （HE） and TUNEL staining. The results suggested that the demyelinating changes in brain tissues infected with CDV mainly related to the metabolic disorder and apoptosis of ogliodendrocytes and astrocytes; also involved with the local disturbance of blood circulation and some neuron lost.

关键词： acute canine distemper demyelinating encephalopathy oligodendrocyte astrocyte apoptosis

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Histopathogenesis of demyelinating encephalopathy on dogs with natural canine distemper

Histopathogenesis of demyelinating encephalopathy on dogs wi...

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中国畜牧兽医学会兽医病理学分第12次暨中国动物病理生理...

作者： Takashi YOSHIKAWA Department Veterinary Pathology School of Veteribary Medicine and Animal Scienceskitasato University35-1 Higashi-23 banchoTowada cityAomori 034- 8628Japan

Up to now, the pathogenesis of dimyelination in brain tissues has remained unclear, although a lot of studies about neuron and glia changes have been done extensively. To further investigate the relation of variety cell in brain tissue to demyelination, this experiment was performed on 12 dogs with canine distemper. To detect the lesions carefully the brain tissue was divided into three portions and eleven slices that were including cerebrum (four slices) , cerebral stem (five slices) and cerebellum (two slices) according to anatomical relation. The sections stained by HE were observed under light microscope. The sections (no, mild, moderate and severe demyelinatig tissues) were selected respectively and stained by immunohistochemistry. The results demonstrated that the disturbance of blood circulation mainly included congestion, edema, thrombus formation, and the occurrence of diffusive intravascular coagulation (DIC). Oli-godendrocytes were occurred metabolic disorders and apoptosis in demyelinating areas. With anti - CDV antigen staining astrocytes were often displayed as intense positive reaction. With anti - GFAP staining, the fibrous astrocytes were more intense positive reaction in acute, mild and moderate demyelinating area, apoptotic astrocytes were detected in lesion foci . Some ependymal cells were infected by CDV. A lot of inclusion bodies and apoptotic cells were revealed. A few of nervous cells were degeneration and shrunken with pyknotic nuclei. According to the results, it was considered that the demyelinating changes in brain tissues related mainly to the disturbance of circulation and metabolic disorder and apoptosis of ogliodendrocyles. On the whole, the causation of demyelination of brain tissues was a common active result by many factors, but not only one.

关键词： Histopathogenesis canine distemper astrocyte oligodendrocyte demyelinating encephalopathy

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