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Association between delayed cardioprotection of aged rat myocytes and activation of mitogenactivated protein kinase

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Chinese Medical Journal 2000年第1期113卷 5-9页

作者：刘秀华王士雯武旭东唐朝枢 Institute of Geriatric Cardiology Chinese PLA General Hospital Beijing 100853 China.

Objective To study the cardioprotective effects of hypoxic preconditioning (HPC) on aged rat ventricular myocytes and the cellular mechanism of protection Methods In the model of hypoxia/reoxygenation (H/R) of isolated ventricular myocytes of aged rat, the effects of HPC on aged rat ventricular myocytes against lethal H/R stimulated ischemia/reperfusion (I/R) injury 24 hours later and the changes of mitogen activated protein kinase (MAPK) system were observed in the present study Results HPC attenuated the lactate dehydrogenase (LDH) release and ATP depletion in myocytes and increased the viability of myocytes It was also found that MAPK and its down stream kinase—S6 kinase were also activated after HPC Conclusion There is delayed cardioprotection in cardiac myocytes of aged rat and the cellular mechanism underlying might involve the activation of MAPK system

关键词： hypoxic preconditioning delayed cardioprotection mitogen activated protein kinase aged rat

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DJ-1 is required for ischemic preconditioning to attenuate ischemia/reperfusion-induced oxidant stress and to elicit delayed cardiopro tection

DJ-1 is required for ischemic preconditioning to attenuate i...

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第八届海峡两岸心血管科学研讨会

作者： He-ping Chen,Hai-shan Lu,Song Wang,Qi-ren Huang,Ming He~* (Department of Pharmacology & Molecular Therapeutics,Nanchang University School of Pharmaceutical Science,Nanchang,330006,China)

＜正＞Ischemic preconditioning（IPC） is a powerful phenomenon that can attenuate ischemia/reperfusion （I/R） - induced oxidant stress and elicit delayed *** mechanisms involve upregulation of the protective antioxidant *** - 1 protein,as a multifunctional intracellular protein,plays an important role in regulating cell survival and anti - oxidant ***,it remains to be clarified whether DJ - 1 is involved in delayed cardioprotection of IPC and plays a pivotal role in anti - oxidant stress of *** study this,rat heart - derived H9c2 cells were exposed to ischemic（ischemic medium for 20 min） preconditioning. Twenty -four hours later cells were subjected to a simulated ischemia/reperfusion（sI/R）—culturing for 3 h in ischemic medium,followed by 2 - h reperfusion in normal medium—and then cell viability,lactate dehydrogenase （LDH）,antioxidant enzymes（superoxide dismutase,catalase and glutathione peroxidase）,malondialdehyde （MDA）,intracellular reactive oxygen species（ROS）,and DJ - 1 protein were measured appropriately. The results showed that IPC efficiently attenuated sI/R - induced viability loss and LDH leakage. Additionally,IPC up - regulated DJ - 1 protein expression,inhibited sI/R - induced the elevation of ROS and MDA contents followed by the increase of antioxidant enzymes（superoxide dismutase,catalase and glutathione peroxidase） ***,when DJ - 1 expression was specifically knocked down by siRNA,the delayed cardioprotection induced by IPC was abolished,and the inhibitory effect of IPC on sI/R - induced oxidant stress was also *** data indicate that DJ - 1 mediates the delayed cardioprotective effect of IPC against sI/R ***,IPC is required for IPC to inhibit sI/R - induced oxidant stress.

关键词： DJ-1 protein Ischemic preconditioning delayed cardioprotection Oxidant stress

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