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Molecular mechanism of dehydrin in response to environmental stress in plant

Progress in Natural Science:Materials International 2007年第3期17卷 237-246页

作者： ZHANG Yuxiu , WANG Zi and XU Jin (Department of Bioengineering, School of Chemical and Environmental Engineering, China University of Mining and Technology (Beijing), Beijing 100083, China Department of Biology, Graduate University of Chinese Academy of Sciences, Beijing 100049, China) [a]Department of Bioengineering School of Chemical and Environmental Engineering China University of Mining and Technology (Beiing) Beijing China [b]Department of Biology Graduate University of Chinese Academy of Sciences Beijing China

dehydrins, known as the D-11 subgroup of late embryogenesis abundant (LEA) protein, are an immunologically distinct family of proteins, which typically accumulate in desiccation-tolerant seed embryo or in vegetative tissues in response to various environmental stresses such as drought, salinity and freezing. The existence of conservative sequences designated as K, S, and Y segments is a structural feature of dehydrins, and the K segment found in all dehydrins represents a highly conserved 15 amino acid motif (EKKGIMDKIKEKLPG) and forms an amphiphilicα-helix. According to the arrangement of these domains and clustering analysis, dehydrins are subdivided into 5 subtypes: YnSK, Kn, KnS, SKn and YnK. Different types of dehydrins are induced by different environmental stress in plants. Study results showed that dehydrins might play important protective roles under abiotic stress via a number of different mechanisms, including improving or protecting enzyme activities by the cryoprotective activity in responding to freeze/thaw or dehydration; stabilizing vesicles or other endomembrane structures by function as the membrane stabilizer during freeze induced dehydration, and preventing the membrane system from the oxidative damage induced by reactive oxygen radicals as the radical scavenger. Here, the gene expression and molecular mechanisms of dehydrin in response to stress in plants are discussed.

关键词： dehydrin stress mechanism.

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In vivo protective effect of late embryogenesis abundant protein(ApSK3 dehydrin)on Agapanthus praecox to promote post-cryopreservation survival

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BIOCELL 2022年第11期46卷 2507-2515页

作者： TINGTING HUANG SHAN DENG JIANGYUAN SHENG DI ZHANG Department of Landscape Science and Engineering School of DesignShanghai Jiao Tong UniversityShanghai200240China Institute for Agri-Food Standards and Testing Technology Shanghai Academy of Agricultural SciencesShanghai201403China

dehydrins(DHNs),as members of the late embryogenesis abundant protein family,play critical roles in the protection of seeds from dehydration and plant adaptation to multiple abiotic *** is a basic method in plant cryopreservation and is characterized by forming a glassy state to prevent lethal ice crystals produced during cryogenic *** this study,ApSK3 type DHN was genetically transformed into embryogenic calluses(EC)of Agapanthus praecox by overexpression(OE)and RNA interference(RNAi)techniques to evaluate the in vivo protective effect of DHNs during *** cell viability showed a completely opposite trend in OE and RNAi cell lines,the cell relative death ratio was decreased by 20.0%in ApSK3-OE EC and significantly increased by 66.15%in ApSK3-RNAi cells after *** of ApSK3 increased the content of non-enzymatic antioxidants(AsA and GSH)and up-regulated the expression of CAT,SOD,POD,and GPX genes,while ApSK3-RNAi cells decreased antioxidant enzyme activities and FeSOD,POD,and APX genes expression during *** findings suggest that ApSK3 affects ROS metabolism through chelating metal ions(Cu^(2+)and Fe^(3+)),alleviates H_(2)O_(2)and OH·excessive generation,activates the antioxidant system,and improves cellular REDOX balance and membrane lipid peroxidation damage of plant cells during *** can effectively improve cell stress tolerance and have great potential for in vivo or in vitro applications in plant cryopreservation.

关键词： dehydrin Embryogenic callus Cryopreservation RNAi Gene overexpression

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Improvement of Drought Tolerance in Transgenic Tobacco Plants by a dehydrin-Like Gene Transfer

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Agricultural Sciences in China 2004年第8期3卷 575-583页

作者： SHENYe JIAWei-long ZHANGYan-qin HUYuan-lei WUQi LINZhongping NationalKeyLaboratoryofProteinEngineeringandPlantGeneEngineering PekingUniversityBeijing100871P.R.China ResearchCenterforDrought ShanxiAcademyofAgricultureSciencesTaiyuan030031P.R.China

A full-length cDNA of dehydrin BcDh2 from Boea crassifolia and its antisense nucleotidesequence have been transferred into tobacco (Nicotiana tabacum) NC89 under the controlof a caulifower mosaic virus 35S promoter. Under a progressive water stress, photosyntheticrate, transpiration rate and stomatal conductance of the sense and antisense plantsreduced, and those of the control reduced much more. Photosynthetic rate, transpirationrate and stomatal conductance of all plants tested increased significantly 24 hourslater after recoveried water supply, and those of the sense and antisense plants werehigher than control. These indicated that overexpression of a dehydrin gene in tobaccomay improve tolerance to water stress for plants, however, antisense BcDh2 gene intransgenic plant did not influence physiological conditions. The results of germinationexperiment of the transgenic seeds showed that on MS medium with different concentrationPEG (8000), sense seed could more endure drought than control, while antisense seed wassensitive to drought. The results suggested that the overexpression of a dehydrin genein tobacco might improve the tolerance to water stress for plants.

关键词： dehydrin Sense Antisense Drought tolerance

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Protein Profiles and dehydrin Accumulation in Some Soybean Varieties (<i>Glycine max</i>L. Merr) in Drought Stress Conditions

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American Journal of Plant Sciences 2013年第1期4卷 134-141页

作者： Estri Laras Arumingtyas Evika Sandi Savitri Runik Dyah Purwoningrahayu Biology Department Faculty of Science and Technology Universitas Islam Negeri (UIN) Maulana Malik Ibrahim Malang Malang Indonesia Biology Department Faculty of Science University of Brawijaya Malang Indonesia Research Institute of Legume and Tuber Crops Malang Indonesia

Drought is one of environmental stresses which the most limiting to plant growth and productivity. Drought stress led to a series of changes including biochemical changes like accumulation of osmolit and specific proteins involved in stress tolerance. One of the proteins that play a role in the mechanism of drought resistance is dehydrin protein. This study aimed to identify the protein profiles and dehydrin accumulation in 7 varieties of local Indonesian soybeans: Tanggamus, Nanti, Seulawah and Tidar (tolerant), Wilis and Burangrang (moderate) and Detam-1 (drought stress sensitive). Plants were treated with drought stress by adjusting soil water content to 25% below field capacity and compared with plants which were grown on normal condition as control plants. The results of SDS-PAGE electrophoresis showed a new protein with the molecular weight of 13 and 52 kDa were induced in Tanggamus, Nanti, Seulawah and Tidar varieties. Western blotting analysis for dehydrin showed that the quantity of the protein in the leaves of all varieties except Tanggamus decreased in drought stress conditions. The quantity of dehydrin protein in tolerant varieties higher than the protein quantity in both moderate varieties and drought sensitive.

关键词： dehydrin Protein Drought Stress Soybean Varieties LEA

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Cloning and expression pattern of a dehydrin-like BDNI gene from drought-tolerant Boea crassifolia Hemsl.

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Chinese Science Bulletin 2000年第22期45卷 2072-2077页

作者： ZHAO Huiwu, LIU Han, YU Haiyuan, HU Yuanlei, GAO Yin, LI Zhenyu & LIN ZhongpingNational Laboratory of Protein Engineering and Plant Genetic Engineering , Peking University, Beijing 100871, China Botanical Institute, Chinese Academy of Sciences, Beijing 100093, China Correspondence should be addressed to Lin Zhongping

A 500-bp cDNA fragment was amplified via RT-PCR from drought-induced total RNA of the drought-tolerant B. crassifolia Hemsl. using primers based on the sequence of published dehydrin conserved region. By using 5’ RACE, full-length coding region (1 148 bp) of BDN1 gene was produced. It is a new member of the dehydrin gene family. Southern analysis indicated that BDN1 is present in the 6. crassifolia genome as a single-copy gene. Northern analysis revealed that its expression is inducible by drought and cold stresses as well as ABA application.

关键词： drought-tolerant plant Boea crassifolia Hemsl. dehydrin 5’ RACE BDN1 gene.

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Molecular Cloning and Functional Characterization of the dehydrin(IpDHN) Gene from Ipomoea Pes-caprae

Molecular Cloning and Functional Characterization of the Deh...

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中国植物学会第十六次全国会员代表大会暨85周年学术年会

作者： Hui Zhang Jie-Xuan Zheng Hua-Xiang Su Kuai-Fei Xia Shu-Guang Jian Mei Zhang Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement South China Botanical GardenChinese Academy of Sciences University of the Chinese Academy of Sciences Key Laboratory of Applied Botany South China Botanical GardenChinese Academy of Sciences

dehydrin(DHN) genes can be rapidly induced to offset water deficit stresses in ***,we reported on a dehydrin gene(IpDHN) related to salt tolerance isolated from Ipomoea pes-caprae L.(Convolvulaceae).The IpDHN protein shares a high amino acid sequence similarity with Arabidopsis dehydrin ERD14(At1 g76180).IpDHN was shown to have a cytoplasmic localization *** RT-PCR analyses showed that IpDHN was constitutively expressed in different organs of ***-caprae plants,and its expression level increased after salt,osmotic stress,oxidative stress,cold stress and ABA *** of the 974-bp promoter of IpDHN identified distinct cis-acting regulatory elements,including an MYB binding site(MBS),ABRE(ABA responding)-elements,Skn-1 motif,and TC-rich *** induced expression of IpDHN in Escherichia coli indicated that IpDHN might be involved in salt,drought,osmotic,and oxidative *** also generated transgenic Arabidopsis lines that over-expressed IpDHN,and we carried out salt and drought stress *** transgenic Arabidopsis plants showed a significant increase in tolerance to salt/drought stresses and less accumulation of hydrogen peroxide(HO) and the superoxide radical(O),accompanied by increasing activity of the antioxidant enzyme system in *** osmotic stresses,the overexpression of IpDHN in Arabidopsis can elevate the expression of ROS-related and stress-responsive genes and can improve the ROSscavenging *** results indicated that IpDHN is involved in cellular responses to salt and drought through a series of pleiotropic effects that are likely involved in ROS scavenging and therefore influence the physiological processes of microorganisms and plants exposed to many abiotic stresses.

关键词： Ipomoea pes-caprae L. dehydrin salt drought promoter

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中华补血草和中亚滨藜叶片中盐激蛋白和dehydrin的研究

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山东师范大学学报（自然科学版） 1999年第4期14卷 422-425页

作者：王洪燕刘箭石凤山东师范大学生物学系山东济南250014 山东师范大学测试中心山东济南250014

应用二相电泳方法,分析中华补血草Limonium sinensis(Girard.) ***.和中亚滨藜AtriplexcentralasiaticaIljin 这二种典型泌盐植物叶片中的蛋白组成变化,发现盐胁迫时,二种植物叶片中均有少量的盐激蛋白出现,其中中亚滨藜叶片中的蛋白质... 详细信息

应用二相电泳方法,分析中华补血草Limonium sinensis(Girard.) ***.和中亚滨藜AtriplexcentralasiaticaIljin 这二种典型泌盐植物叶片中的蛋白组成变化,发现盐胁迫时,二种植物叶片中均有少量的盐激蛋白出现,其中中亚滨藜叶片中的蛋白质组成变化大一些.运用Western- blot 方法对这二种盐生植物叶片中的dehydrin蛋白进行检测,结果发现,无论是在正常环境下,还是高浓度盐渍环境下。

关键词：中华补血草中亚滨藜盐激蛋白 dehydrin

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Changes of DHN1 expression and subcellular distribution in *** cells under osmotic stress

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Science China(Life Sciences) 2002年第1期45卷 1-9页

作者：邱全胜王泽宙蔡起贵姜荣锡 College of Life Sciences Beijing Normal University Beijing 100875 China

The changes of DHN1 expression and subcellular distribution in A. delicisoa cells under osmotic stress were studied by using GFP as a reporter molecule. Through creating the Xba I and BamH I restriction sites at the ends of dhn1 by PCR, the expression vector for the fusion protein DHN1-mGFP4 was constructed by cloning dhn1 into plasmid pBIN-35SmGFP4. Then the DHN1-mGFP4 expression vector was transformed into A. delicisoa suspension cells by microprojectile bombardment method. Bright green fluorescence of GFP which shows the high-level expression of DHN1-mGFP4 was visualized after culture for 10 h. However, the green fluorescence was only located within the nucleus. By increasing the culture medium osmotic potential, the green fluorescence was visualized in the cytoplasm (mainly around the plasma membranes). The generation of GFP fluorescence in the cytoplasm was also promoted by increasing the medium osmotic potential. Moreover, GFP green fluorescence was abolished by protein synthesis inhibitor dicyclohexylcarbodiimid, indicating that the cytoplasmic DHN1 was newly synthesized under osmotic stress. Furthermore, ABA promoted the presence of green fluorescence in the cytoplasm, and the GFP fluorescence was visualized within a shorter time under a higher osmotic potential.

关键词： A. delicisoa suspension cells, osmotic stress, dehydrin DHN1, green fluorescent protein (GFP), transient expression, subcellular distribution, ABA.

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Ectopic expression of antifreeze protein gene from Ammopiptanthus nanus confers chilling tolerance in maize

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The Crop Journal 2021年第4期9卷 924-933页

作者： Yuanyuan Zhang Yang Cao Hongying Zheng Wenqi Feng Jingtao Qu Fengling Fu Wanchen Li Haoqiang Yu Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region Ministry of AgricultureMaize Research InstituteSichuan Agricultural UniversityChengdu 611130SichuanChina College of Life Science&Biotechnology Mianyang Teachers’CollegeMianyang 621000SichuanChina

Improved chilling tolerance is important for maize production. Previous efforts in transgenics and marker-assisted breeding have not achieved practical results. In this study, the antifreeze protein(AnAFP) from the super-xerophyte Ammopiptanthus nanus was aligned to KnS-type *** in vitro and subcellular localization showed that AnAFP was phosphorylated by maize casein kinase II and translocated from nucleus to cytoplasm under chilling stress. AnAFP also increased lactate dehydrogenase activity. A parent line of commercial maize hybrids was transformed with the AnAFP gene. Based on thermal asymmetric interlaced PCR, one hemizygous and two homozygous integration sites were identified in one T_(1) line. Ectopic expression of AnAFP in transgenic lines was confirmed by quantitative real-time PCR, RNA-seq, and Western blotting. After chilling treatment, the transgenic lines showed significantly improved phenotype, higher kernel production, survival rate and biomass, and lower relative electrolyte leakage and superoxide dismutation than the untransformed line. Thus, ectopic expression of AnAFP gene improved chilling tolerance in the transgenic lines, which could be used to apply for further safety assessment for commercial breeding.

关键词： Antifreeze protein Chilling tolerance dehydrin Ectopic expression Homozygosity identification

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