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Acquisition of LURE-Binding Activity at the Pollen Tube Tip of Torenia fournied

Molecular Plant 2013年第4期6卷 1074-1090页

作者： Satohiro Okuda Takamasa Suzuki Masahiro M. Kanaoka Hitoshi Moric Narie Sasaki Tetsuya Higashiyama Division of Biological Science Graduate School of Science Nagoya University Furo-cho Chikusa-ku Nagoya Aichi 464-8602 Japan JST ERATO Higashiyama Live-Holonics Project Nagoya University Furo-cho Chikusa-ku Nagoya Aichi 464-8602 Japan department of Bioengineering Sciences Graduate School of Bioagricultural Sciences Nagoya University Furo-cho Chikusa-ku Nagoya Aichi 464-8602 Japan WPI-ITbM Nagoya University Furo-cho Chikusa-ku Nagoya Aichi 464-8602 Japan

Pollen tube guidance is controlled by multiple complex interactions with the female tissues. Here, we show that pollen tubes of Torenia fournieri are regulated by a stylar tissue in a length-dependent manner to receive and respond to attractant LURE peptides secreted from synergid cells. We developed an immunostaining method to visualize LURE peptides bound at the plasma membrane of the tip region of the pollen tube. Using this method, we found that LURE peptides bound specifically to pollen tubes growing through a cut style. The peptides also bound to pollen tubes growing through a shorter style, which were not competent to respond to these peptides. These observations suggested a possibility that acquisition of the LURE peptide reception ability and acquisition of full competency are separable pro- cesses. RNA-Seq suggested that the transcription profile of pollen tubes was affected by both the length of the style and the cultivation period, consistently with physiological changes in binding activity and LURE response ability. The data- base generated from de novo RNA-Seq of Torenia pollen tubes was shown to be useful to identify pollen tube proteins by mass spectrometry. Our studies provide insight and an effective platform for protein identification to understand pollen tube guidance.

关键词： pollen tube guidance attractants pollen tube de novo transcriptome proteome.

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transcriptome profiling of desmos chinensis: revealing the molecular basis of dipartite perianth evolution in the early divergent angiosperm family Annonaceae

Transcriptome profiling of Desmos chinensis: revealing the m...

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中国植物学会第十六次全国会员代表大会暨85周年学术年会

作者： Amy Wing-Sze Leung Sangtae Kim Richard Mark Kingsley Saunders School of Biological Sciences The University of Hong Kong department of Biology Sungshin Women's University

Although petals are not homologous within angiosperms,different types of petals might nevertheless share a deep homology at the molecular level.A dipartite perianth（with differentiated sepals and petals） has evolved independently in the early divergent angiosperm family Annonaceae and some core eudicots,with the development of bracteopetals and andropetals *** is known of the molecular control of floral development in the non-model family *** order to better understand floral developmental genetics of the family,we profiled the transcriptome of a representative species,desmos chinensis,during its floral development using high throughput RNA-seq technique.A detailed developmental study extending from small buds until post-anthesis petal abscission was performed to correlate morphological and anatomical changes of floral organs during *** organ and leaf samples at developing and mature stages were obtained for transcriptome *** total,22,112 assembled transcripts（c.80% transcriptome completeness） were recovered using de novo *** transcriptome was annotated and characterized based on publicly available *** expression level estimation and gene ontology enrichment analysis suggested that a large proportion of *** transcripts are responsible for defense,with some expressed strongly in *** related transcripts,including those responsible for glycolysis,were found to be up-regulated in mature petals.33 homeotic MADS-box gene transcripts were extracted from the floral and leaf *** orthology of these putative MADS-box transcripts were validated using phylogenetic analyses,OrthoMCL and UniProt *** most of the type II MADS-box genes are broadly expressed in all floral organs,the floral organ-specific RNA-seq data revealed that the B-class APETALA3（AP3） and E-class SEPALATA3（SEP3） gene expressions are specifically absent from *** thereby hypothesized

关键词： Annonaceae desmos chinensis RNA-seq de novo transcriptome MADSbox genes floral developmental genetics dipartite perianth evolution

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