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Identification and characterization of three new flavonoids from Rhododendron dauricum

Chinese Journal of Natural Medicines 2015年第8期13卷 628-633页

作者： LOU Xin-Wei LIN Qing-Hua ZHANG Guan-Yu LIU Wen-Yuan FENG Feng QU Wei Department of Natural Medicinal Chemistry China Pharmaceutical University Department of Pharmaceutical Analysis China Pharmaceutical University Key Laboratory of Drug Quality Control and Pharmacovigilance China Pharmaceutical UniversityMinistry of Education Key Laboratory of Carcinogenesis and Intervention China Pharmaceutical University

The present study was designed to determine the major chemical constituents of the leaves of Rhododendron dauricum L. Compounds were isolated and purified by various chromatographic methods, and their structures were elucidated by physicochemical properties and spectral data. The present study identified two new C-methyl flavanones, 5, 7, 3', 5'-tetrahydroxy-6, 8-di-C-methyl flavanone(1) and 5, 4'-dihydroxy-8-C-methylflavanone-7-O-β-D-glucopyranoside(2), and one new flavonoid glycoside, quercetin-3-O-β-D-(6''-O-cinnamoyl)-galactoside(3), along with seven known compounds, including syzalterin(4), poriolin(5), farrerol-7-O-β-D-glucopyranoside(6), myrciacetin(7), quercetin-3-O-β-D-(6-p-hydroxy-benzoyl)-galactoside(8), quercetin-3-O-β-D-(6-p-coumaroyl)-galactoside(9), and 5, 7, 3', 5'-tetrahydroxyl flavanone(10). Compounds 1-3 were determined to be new flavonoids; compounds 4-6 were isolated from this species for the first time; and compounds 7-10 were reported for the first time from this genus.

关键词： Rhododendron dauricum L Flavonoids Spectroscopic identification

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兴安杜鹃总黄酮抗氧化活性

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湖北农业科学 2014年第8期53卷 1895-1897页

作者：王姝涵李玉桃孙墨珑东北林业大学理学院哈尔滨150040

为考察兴安杜鹃(Rhododendron dauricum L.)总黄酮对·OH、超氧阴离子自由基和亚硝酸盐的清除作用,以及对亚硝胺合成的阻断作用,采用超声波提取法,以95%(V/V)乙醇浸提兴安杜鹃叶和茎样品,紫外可见分光光度法测定兴安杜鹃提取物的总黄酮... 详细信息

为考察兴安杜鹃(Rhododendron dauricum L.)总黄酮对·OH、超氧阴离子自由基和亚硝酸盐的清除作用,以及对亚硝胺合成的阻断作用,采用超声波提取法,以95%(V/V)乙醇浸提兴安杜鹃叶和茎样品,紫外可见分光光度法测定兴安杜鹃提取物的总黄酮含量。结果表明,兴安杜鹃提取物对·OH、亚硝酸盐和超氧阴离子均有清除作用,对亚硝胺的合成具有阻断作用,且随着浓度的增大,清除或合成阻断作用增加。兴安杜鹃提取物对超氧阴离子的清除效果优于对·OH和亚硝酸盐的清除效果,叶提取物的清除作用强于茎提取物的清除作用。

关键词：兴安杜鹃(Rhododendron dauricum L ) 总黄酮抗氧化活性清除作用

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Molecular Identification of Chinese Materia Medica and Its Adulterants Using ITS2 and <i>psbA-trnH</i>Barcodes: A Case Study on Rhizoma Menispermi

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Chinese Medicine 2014年第4期5卷 190-198页

作者： Pei Yang Xiwen Li Hong Zhou Hao Hu Hui Zhang Wei Sun Yitao Wang Hui Yao Institute of Medicinal Plant Development Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences Beijing China State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical Sciences University of Macao Macao China Development Center of Traditional Chinese Medicine and Bioengineering Changchun University of Chinese Medicine Changchun China

Rhizoma Menispermi, derived from the rhizoma of Menispermum dauricum DC., is one of the most popular Chinese medicines. However Rhizoma Menispermi is often illegally mixed with other species in the herbal market, including Aristolochia mollissimae Hance, which is toxic to the kidneys and potentially carcinogenic. The use of DNA barcoding to authenticate herbs has improved the management and safety of traditional medicines. In this paper, 49 samples belonging to five species, including 34 samples of M. dauricum, from different locations and herb markets in China were collected and identified using DNA barcoding. The sequences of all 34 samples of Rhizoma Menispermi are highly consistent, with only one site variation in internal transcribed spacer 2 (ITS2) of nuclear ribosomal DNA and no variations in the psbA-trnH region. The intra-specific genetic distance is much smaller than inter-specific one. Phylogenetic analysis shows that both sequences allow the successful identification of all species. Nearest distance and BLAST1 methods for the ITS2 and psbA-trnH regions indicate 100% identification efficiency. Our research shows that DNA barcoding can effectively distinguish Rhizoma Menispermi from its adulterants from both commercial and original samples, which provides a new and reliable way to monitor commercial herbs and to manage the modern medicine market.

关键词： Rhizoma Menispermi Menispermum dauricum DC. ITS2 psbA-trnH Identification Adulterants

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