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Cynodon dactylon extract ameliorates cognitive functions and cerebellar oxidative stress in whole body irradiated mice

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Asian Pacific Journal of Tropical Biomedicine 2019年第7期9卷 278-283页

作者： Roopesh Poojary Nayanatara Arun Kumar Reshma Kumarchandra Vinodini NA Bhagyalakshmi K Ganesh Sanjeev Department of Physiology Kasturba Medical CollegeMangaloreManipal Academy of Higher EducationManipalKarnatakaIndia Department of Biochemistry Kasturba Medical CollegeMangaloreManipal Academy of Higher EducationManipalKarnatakaIndia Microtron Centre Department of Studies in PhysicsMangalore UniversityMangalagangotriKarnatakaIndia

Objective: To explore the effect of hydroalcoholic extract of Cynodon dactylon on the whole body radiation-induced oxidative status of the cerebellum and cognitive impairments in mice. Methods: Swiss albino mice were randomly divided into the control group, radiation control group, low dose and high dose Cynodon dactylon extract treated groups and pre-treated with Cynodon dactylon extract before irradiation. Cynodon dactylon extract was administered for 7 d daily in low dose(0.25 g/kg) and high dose(1 g/kg). On day 7, mice were irradiated with a sublethal dose of 5 Gy gamma rays. Motor coordination was assessed by elevated rotarod test and spatial memory was studied by water maze test. Subsequently, biochemical markers(glutathione, lipid peroxidation and nitric oxide levels) in the cerebellum were evaluated. Results: The gamma irradiated group showed significant impairment in motor coordination and spatial memory compared to normal mice. Mice treated by Cynodon dactylon extract prior to gamma radiation showed good improvement in both paradigms compared to the radiation control group. Moreover, glutathione level was increased, while lipid peroxidation and nitric oxide levels were significantly reduced in mice receiving low dose and high dose of Cynodon dactylon extract compared to the radiation control group. Conclusions: The present study suggests the neuroprotective role of Cynodon dactylon against radiation-induced cognitive impairment and oxidative stress on the cerebellum of mice.

关键词： Cerebellum Radiation Cognition Cynodon dactylon Memory

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Anti-chikungunya activity of luteolin and apigenin rich fraction from Cynodon dactylon

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Asian Pacific Journal of Tropical Medicine 2015年第5期8卷 352-358页

作者： Krishnan Saravana Murali Srinivasan Sivasubramanian Savariar Vincent Shanmugaraj Bala Murugan Bupesh Giridaran Sundaram Dinesh Palani Gunasekaran Kaveri Krishnasamy Ramalingam Sathishkumar Plant Genetic Engineering Lab Department of Biotechnology.Bharathiar University Department of Virology King Institute of Preventive Medicine and Research Centre for Environmental Research and Development and Loyola Institute of Frontier Energy Loyola College Vellore Institute of Technology Plant Genetic Engineering Lab Department of BiotechnologyBharathiar University

Objective:To obtain Iuteolin and apigenin rich fraction from the ethanolic extract of Cynodon dactylon(L.)(***) Pers and evaluate the fraction's cytotoxicity and anti-Chikungunya potential using Vero ***:The ethanolic extract of *** was subjected to silica gel column chromatography to obtain anti-chikungunya virus(CHIKV) *** phase-HPLC and GC-MS studies were carried out to identily the major phytochemicals in the fraction using phylochemical *** and the potential of the fraction against CHIKV were evaluated in vitro using Vero *** in viral replication was assessed by reverse transcriptase-polymerase chain reaction(RT-PCR) after treating the viral infected Vero cells with the ***:Reverse Phase-HPLC and GC-MS studies confirmed the presence of flavonoids,luteolin and apigenin as major phytochemicals in the anti-CHIKV ethanolic fraction of ***- The fraction was found to exhibit potent viral inhibitory activity(about 98%) at the concentration of 50 μg/mL as observed by reduction in cytopathic effect,and the cytotoxic concentration of the fraction was found to be 250 μg/***-PCR analyses indicated that the reduction in viral mRNA synthesis in fraction treated infected cells was much higher than the viral infected control ***:Luteolin and apigenin rich ethanolic fraction from *** can be utilized as a potential therapeutic agent against CHIKV infection as the fraction does not show cytotoxicity while inhibiting the virus.

关键词： Antiviral activity Chikungunya virus Cynodon dactylon Flavonoids Luteolin Apigenin

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Heritability estimates for seed yield and its components in Cynodon dactylon var. dactylon (L.) Pers.

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Grass Research 2022年第1期2卷 48-53页

作者： Chengcheng Tan Yanqi Wu Charles M.Taliaferro Greg E.Bell Dennis L.Martin Mike W.Smith Department of Plant and Soil Sciences Oklahoma State UniversityStillwaterOK 74078USA Oklahoma Department of Agriculture Food and Forestry2800 N.Lincoln Blvd.Oklahoma CityOK 73105USA Department of Horticulture and Landscape Architecture Oklahoma State UniversityStillwaterOK 74078USA

Bermudagrass[Cynodon dactylon ***(L.)Pers.]is a major warm-season turf and forage grass *** yield is an important trait targeted for improvement in bermudagrass breeding programs because of the increased interest in seed-propagated *** the nature of genetic variation for seed yield and its components in bermudagrass would aid the development of seed-propagated *** objective of this study was to estimate the genetic variation and narrow-sense heritability for seed yield and its two major components,inflorescences prolificacy and seed set percentage in ***-five half-sib families and their respective clonal parents were evaluated at two Oklahoma locations,Perkins and Stillwater(Oklahoma,USA),over two ***-sib families were different for seed yield,inflorescences prolificacy and seed set percentage,indicating the expression of additive genes in controlling these ***×location effects were observed for seed set percentage and seed *** three traits showed family×year interaction *** was a significant family×location×year interaction in inflorescences prolificacy and seed set ***-sense heritability estimates for seed yield was 0.18 based on variance component analysis among half-sib families and ranged from 0.26 to 0.68 based on parent-offspring regressions,indicating genetic complexity of seed *** estimates were moderate(0.30−0.55)for inflorescences prolificacy and moderate to relatively high(0.41−0.78)for seed set *** results indicate that sufficient magnitudes of additive genetic variation for seed set percentage and inflorescence prolificacy permit positive response to selection and conventional progeny-based genotypic evaluation is necessary for seed yield ***:Tan C,Wu Y,Taliaferro CM,Bell GE,Martin DL,et *** estimates for seed yield and its components in Cynodon dactylon var.

关键词： dactylon cultivar breeding

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Antidiabetic and antidiarrhoeal potentials of ethanolic extracts of aerial parts of Cynodon dactylon Pers

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Asian Pacific Journal of Tropical Biomedicine 2015年第8期5卷 642-645页

作者： Md.Saidur Rahman Rasheda Akter Santosh Mazumdar Faridul Islam Nusrat Jahan Mouri Nemai Chandra Nandi Abu Syeed Mohammad Mahmud Bangladesh Council of Scientific and Industrial Research Laboratories Chittagong

Objective: To explore the antidiabetic and the antidiarrhoeal effects of ethanolic extracts of Cynodon dactylon Pers. aerial parts(EECA) in Wistar ***: To assess the antidiabetic activity of EECA, oral glucose tolerance test(OGTT) model and alloxan induced diabetic test(AIDT) model were performed. The EECA was used at the doses of 2 g/kg, 1 g/kg and 500 mg/kg body weight in OGTT model and 1.5 g/kg was used for AIDT model. Castor oil-induced diarrhoeal model and gastrointestinal motility test with barium sulphate milk model were performed for evaluating the antidiarrhoeal effects at doses of 1 g/kg, 750 mg/kg ***: The dose 2 g/kg in OGTT and 1.5 g/kg in AIDT model blood glucose levels decreased significantly(P < 0.01) in Wistar rats that showed antidiabetic effect of EECA. After administration of EECA at the dose of 1 g/kg, the extract showed significant(P < 0.05) antidiarrhoeal activity in castor oil-induced diarrhoeal model. The results were also significant(P < 0.01) in barium sulphate milk model for the same dose by using above mentioned ***: It is concluded that EECA contains both antidiabetic and the antidiarrhoeal properties.

关键词： Cynodon dactylon Ethanolic extracts Antidiabetic Antidiarrhoeal Wistar rats

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狗牙根EST-SSR反应体系优化及其遗传多样性初步分析

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新疆农业大学学报 2014年第2期37卷 112-118页

作者：帕提古丽·麦麦提敏张延辉李培英阿不来提新疆草地资源与生态自治区重点实验室新疆农业大学草业与环境科学学院乌鲁木齐830052

利用 L16（4）5正交实验设计,对新疆狗牙根 EST-SSR反应体系进行优化；并将该体系初步应用于14份新疆典型区域的野生狗牙根材料,通过聚类分析研究供试材料的亲缘关系.结果表明,新疆狗牙根 EST-SSR反应最优体系为：20μL反应体系中含模板... 详细信息

利用 L16（4）5正交实验设计,对新疆狗牙根 EST-SSR反应体系进行优化；并将该体系初步应用于14份新疆典型区域的野生狗牙根材料,通过聚类分析研究供试材料的亲缘关系.结果表明,新疆狗牙根 EST-SSR反应最优体系为：20μL反应体系中含模板DNA 100 ng,引物0.5μmol/L,2μL 10×Buffer,MgCl21.5 mmol/L,dNTPs 0.2 mmol/L,Taq DNA聚合酶0.5 U.运用该体系对14份新疆狗牙根材料进行PCR扩增,条带较清晰,可用于新疆狗牙根 EST-SSR标记的进一步研究.对14份狗牙根的多样性及其亲缘关系分析结果表明,狗牙根 Nei’s 基因多样性指数（H）变异范围为0.2077~0.3153,平均为0.2550；Shannon信息指数（I）在0.2540~0.4394.

关键词：狗牙根体系优化多样性 Cynodon dactylon L

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狗牙根居群遗传分化的RAPD扩增片段频率分析

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湖北农业科学 2011年第13期50卷 2706-2709页

作者：梁月香梁慧敏夏阳燕丽萍刘翠兰江苏农林职业技术学院农艺系江苏句容212400 宁夏农林科学院银川750002 山东省林业科学研究所济南250014

狗牙根(Cynodon dactylon ***.)不同居群由南向北外部性状具有广泛的变异性,为明确其分化的分子基础,以收集到的28份狗牙根为材料,用20个随机引物(10 bp)共扩增出318个RAPD多态性片段,平均每个引物15.9个多态位点,在24个居群中共检测到... 详细信息

狗牙根(Cynodon dactylon ***.)不同居群由南向北外部性状具有广泛的变异性,为明确其分化的分子基础,以收集到的28份狗牙根为材料,用20个随机引物(10 bp)共扩增出318个RAPD多态性片段,平均每个引物15.9个多态位点,在24个居群中共检测到了46个特异性片段;3种类型有8%的多态性片段明显不同;相似生境或同一地域的居群在一些位点上表现出相似或相同的变化。

关键词：狗牙根(Cynodon dactylon L.Pers.) 遗传分化 RAPD

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3种生境下野生狗牙根几个形态性状的比较

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长江大学学报（自科版）（中旬） 2006年第2期3卷 134-136,100页

作者：李兴祥刘志雄王洋长江大学园艺园林学院湖北荆州434025

以平均节间长、最长节间、节间直径、顶二叶叶长和叶宽5个指标,比较了野生狗牙根(Cynodon dac-tylonL.)在阳生地、阴生地和旱生地3种生境下的形态差异,结果表明:野生狗牙根的平均节间长、最长节间、叶长、叶宽4个指标在3种生境下表现出... 详细信息

以平均节间长、最长节间、节间直径、顶二叶叶长和叶宽5个指标,比较了野生狗牙根(Cynodon dac-tylonL.)在阳生地、阴生地和旱生地3种生境下的形态差异,结果表明:野生狗牙根的平均节间长、最长节间、叶长、叶宽4个指标在3种生境下表现出显著差异,但节间直径没有明显差异;就成坪性、实用性的综合指标而言,旱生狗牙根较强于阳生狗牙根和阴生狗牙根。据此推测野生狗牙根能通过对自身形态结构的调整对不同生境产生可塑性响应,并以此来适应环境胁迫。

关键词：狗牙根(Cynodon dactylon L.) 生境形态特征

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4个狗牙根品种水分利用效率的比较

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长江大学学报（自科版）（中旬） 2007年第3期4卷 37-39页

作者：费永俊廖启蓉曾璇长江大学园艺园林学院湖北荆州434025

比较了生长在植被砼上4个狗牙根(Cynodon dactylonL.)品种(Jackpot、Common、040459/680、Xin-nong No.1)的光合速率、蒸腾速率和水分利用效率。结果表明,4个品种的光合速率的差异性水平高于蒸腾速率和水分利用效率;水分利用效率由高到... 详细信息

比较了生长在植被砼上4个狗牙根(Cynodon dactylonL.)品种(Jackpot、Common、040459/680、Xin-nong No.1)的光合速率、蒸腾速率和水分利用效率。结果表明,4个品种的光合速率的差异性水平高于蒸腾速率和水分利用效率;水分利用效率由高到低的顺序是:Common(3.69)、040459/680(3.56)、Jackpot(3.31)、Xinnong No.1(3.16);光合速率、蒸腾速率、水分利用效率之间有或正或负的相关关系。

关键词：狗牙根(Cynodon dactylon L.) 光合速率蒸腾速率水分利用效率

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