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(PMCP+TTA+damp)三元萃取剂对U(Ⅵ)的协同萃取

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核化学与放射化学 1993年第1期15卷 50-55页

作者：陈金榜王文盈北京大学技术物理系北京100871

研究了1-苯基-3-甲基-4-辛酰基吡唑啉酮-5(PMCP或HA)、2-噻吩甲酰三氟丙酮(TTA或HT)和甲基膦酸二异戊酯(damp或B)三元萃取剂的甲苯溶液,从高氯酸钠底液中对U(Ⅵ)的协同萃取。发现有显著的二元及三元协萃效应。用斜率法测得萃合物及协萃... 详细信息

研究了1-苯基-3-甲基-4-辛酰基吡唑啉酮-5(PMCP或HA)、2-噻吩甲酰三氟丙酮(TTA或HT)和甲基膦酸二异戊酯(damp或B)三元萃取剂的甲苯溶液,从高氯酸钠底液中对U(Ⅵ)的协同萃取。发现有显著的二元及三元协萃效应。用斜率法测得萃合物及协萃物的组成分别为UO_2A_2、UO_2A_2B、UO_2T_2B、UO_2AT及UO_2ATB。其萃取平衡常数及协萃平衡常数分别为lgK_A=-0.42、lgK_(AB)=4.21、lgK_(TB)=3.83、lgK_(AT)=-0.60、lgK_(ATB)=4.27(25℃)。讨论了萃合物及协萃物的可能结构式。

关键词： PMCP TTA damp 协同萃取铀

缺氧缺营养对肿瘤来源自噬小体damp的影响及其诱导IL-10+B细胞的研究

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缺氧缺营养对肿瘤来源自噬小体DAMP的影响及其诱导IL-10+B细胞的...

作者：盛业萌东南大学

学位级别：硕士

肿瘤发生早期,肿瘤组织通过周围正常组织的血液循环系统供给氧气和营养物质,随着肿瘤快速生长,新生血管形成,但其供血供氧能力远远无法满足肿瘤组织快速增殖的要求,因此,肿瘤组织长期处于缺血缺氧状态。在肿瘤组织微环境中,因能量缺乏... 详细信息

肿瘤发生早期,肿瘤组织通过周围正常组织的血液循环系统供给氧气和营养物质,随着肿瘤快速生长,新生血管形成,但其供血供氧能力远远无法满足肿瘤组织快速增殖的要求,因此,肿瘤组织长期处于缺血缺氧状态。在肿瘤组织微环境中,因能量缺乏而坏死的肿瘤细胞会向细胞间或血液循环中释放危险相关模式分子(Danger-associated molecular-pattern, damp),如HMGB1、HSP90等。目前认为,自噬是细胞应对营养缺乏、氧化应激、低氧、感染等恶劣环境因素的生存机制之一,细胞可以通过自噬,直接降解胞内蛋白质大分子回收再利用,抵抗凋亡,在不利环境下存活。我们前期研究表明,肿瘤细胞来源自噬小体(tumor released autophagosome,TRAP)能诱导B细胞成为分泌高水平IL-10、对T细胞应答具有抑制功能的调节性B细胞(IL-10+Breg),但缺氧、缺营养条件是否上调damp的表达、胞内damp是否被包裹入TRAP以及携带不同含量damp的TRAP是否影响IL-10+Breg的诱导还有待进一步研究。目的：1、探讨缺氧、缺营养对肿瘤细胞及其TRAP中damp的影响：2、探讨缺氧、缺营养条件下获得的TRAP对诱导B细胞成为IL-10+B细胞及其功能的影响。方法：1、检测不同细胞培养状态TRAP中是否存在damp及其含量1.1检测正常状态下人肝癌细胞株HepG2、SMMC7721、Huh7来源自噬小体中damp表达水平;1.2检测不同培养条件HepG2细胞中damp表达水平;1.3检测不同培养条件HepG2-TRAP中damp含量;2、检测不同培养条件TRAP对B细胞成为IL-10+B细胞的影响,及对IL-10+B细胞功能的影响2.1将不同培养条件HepG2-TRAP与健康供体B细胞共孵育72小时,检测IL-10+B细胞比例,及培养上清中IL-10水平;2.2将不同培养状态TRAP诱导的IL-10+B细胞加入抗CD3单抗刺激PBMC的体外增殖体系,96小时后检测CD4+T和CD8+T细胞CFSE衰减情况。结果：1、不同培养状态募集的TRAP中damp含量存在差异1.1不同人肝癌细胞株TRAP中damp含量存在差异。通过流式细胞术检测TRAP表面HMGB1含量,我们发现SMMC7721-TRAP 中 HMGB1平均荧光强度(MFI)明显高于Huh7-TRAP和 HepG2-TRAP(102vs31 vs27);Western blot检测TRAP+HMGB1和HSP90含量,通过比较相对灰度值,可知7721-TRAP中HMGB1含量明显高于Huh7-TRAP和 HepG2-TRAP (67%vs45%vs37%),7721-TRAP中HSP90亦高于Huh7-TRAP和 HepG2-TRAP(90%vs68%vs62%)。1.2缺氧上调细胞中damp含量,缺营养上调细胞自噬水平。通过Western blot检测细胞中HMGB1、HSP90、LC3含量,通过比较相对灰度值,可知缺氧条件下细胞中HMGB1的含量较正常条件和缺营养条件明显增加(95%vs50%vs68%),HSP90含量亦高于正常条件和缺营养条件(98%vs 38%vs47%);缺营养条件下细胞中LC3-Ⅱ含量相较于正常条件和缺氧条件明显增加(94%vs 15%vs18%)。1.3缺氧上调TRAP中damp含量。通过质谱分析比对缺氧条件TRAP和正常条件TRAP,我们发现HMGB1 和 HSP90在缺氧条件下含量均增加;通过流式细胞术检测TRAP表面HMGB1含量,我们发现缺氧条件TRAP中HMGB1的平均荧光强度(MFI)明显高于正常条件TRAP(128vs29),缺营养条件TRAP中HMGB1的平均荧光强度(MFI)与正常条件相比无明显差异(31vs29);通过Western blot检测TRAP 中 HMGB1、HSP90含量,通过比较相对灰度值,可知缺氧条件下TRAP中HMGB1的含量较正常条件和缺营养条件明显增加(127%vs30%vs48%), HSP90含量亦高于正常条件和缺营养条件(135%vs76%vs120%)。2、缺氧、缺营养状态下募集的TRAP诱导B细胞产生IL-10及抑制T细胞增殖的能力明显强于普通培养条件下募集的TRAP,其中与TRAP的damp含量相关。2.1缺氧条件TRAP诱导B细胞成为IL-10+B的比例高于缺营养条件TRAP和正常条件TRAP (7.4%vs4.6%vs3.6%);培养上清中IL-10的含量亦高于缺营养条件TRAP和正常条件TRAP(147vs45vs40)(9pg/mL);其产生的IL-10+B对CD8+T细胞增殖功能的抑制也高于缺营养条件TRAP和正常条件TRAP(43.8%vs48.7%vs50.

关键词： TRAP damp 肿瘤微环境 IL-10+B细胞免疫功能

damp对人血液淋巴细胞dCMP脱氨酶抑制作用的动力学研究

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大连医科大学学报 1985年第2期 6-11页

作者：赵宝昌大连医学院生化教研室

本文采用取样测定法对人血液淋巴细胞dCMP脱氨酶活性进行放射性同位素测定。笔者研究了底物dCMP固定于不同浓度时damp对此酶的抑制作用,发现dCMP浓度很低时,低浓度的damp对酶具有激活作用。只有当此酶被dCMP或别构激活剂dCTP饱和时,dAM... 详细信息

本文采用取样测定法对人血液淋巴细胞dCMP脱氨酶活性进行放射性同位素测定。笔者研究了底物dCMP固定于不同浓度时damp对此酶的抑制作用,发现dCMP浓度很低时,低浓度的damp对酶具有激活作用。只有当此酶被dCMP或别构激活剂dCTP饱和时,damp才表现出纯的同构竞争性抑制作用,其抑制常数为4×10;M,此值远低于T型和R型构象对dCMP的米氏常数。笔者对实验结果进行动力学分析揭示,在酶完全转变成R型构象前,damp始终具有激活作用,直到酶完全转变成R型构象后,damp才发挥其纯的同构竞争性抑制作用。动力学分析也进一步证明,dCMP脱氨酶动力学作图的表观复杂性实际上是遵守米氏方程式的两种构象（T型和R型）的综合表现这一论断。

关键词：构象脱氨酶水解酶白细胞淋巴细胞 dCMP damp 米氏方程式解剖学血液

PMBP与damp及TBPO萃取剂对三价钕的协同萃取

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河北大学学报(自然科学版) 1987年第4期 25-30页

作者：孟庆珍马香仁杨月辉北京师院化学系河北大学化学系

本文研究了1-苯基3-甲基4-苯甲酰基吡唑啉酮-5（PMBP）与甲基磷酸二异丁酯（damp）及三丁基氧化磷（TBPO）的甲苯溶液在HCIO4介质中对Nd（Ⅲ）的协同萃取,测得了萃合物的组成及萃取平衡常数。讨论了萃合物可能的结构式及萃取机理。

关键词： PMBP Nd TBPO damp 萃取平衡常数协同萃取溶剂萃取三价稀土萃取剂分析试剂

Preclinical Verification of Modulated Electro-Hyperthermia—Part III. Immunogenic Effects

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International Journal of Clinical Medicine 2024年第7期15卷 335-364页

作者： Andras Szasz Department of Biotechnics Hungarian University of Agriculture and Life Sciences Gdll Hungary

The modulated electro-hyperthermia (mEHT) method is a unique approach that utilizes all the essential apoptotic pathways through an external radiofrequency (RF) signal. The high-frequency RF is amplitude-modulated and coupled capacitively to the target. The provided energy triggers the death receptors and FAS-FADD complexes in the malignant cells. Multi-pathway apoptosis produces immunogenic cell death (ICD). This ICD provides intracellular information about cancer cells by releasing damage-associated molecular patterns (damp), including membrane expression of calreticulin (CRT) and extracellular ATP, HMGB1, and HSP70, executing tumor-specific antigen presentation. The antigen-presenting cells (APCs) play a crucial role in reestablishing immune surveillance and hampering the tumor cells’ ability to hide, thereby evading immune attacks. The matured DCs (generally APCs) produce tumor-specific killer and helper T-cells, which have the potential to be active in distant metastases from the treated location. This unique mechanism of action underscores its potential in cancer treatment and extends the local mEHT treatment to the whole body anticancer therapy with an abscopal effect.

关键词： mEHT Cancer Thermal Nonthermal Immunogenic ICD damp Tumor-Specific Immune

Cosmic-ray Charge Measurement by dampE Plastic Scintillator Detector

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IMP & HIRFL Annual Report 2018年第1期 16-17页

作者： Zhang Yapeng Ding Meng 不详

Precisely measuring the energy spectra of cosmic-rays is vital to constrain the cosmic-ray production mechanism[1]and their propagation in the stellar medium[2].DArk Matter Particle Explorer(dampE)[3]is a high-resolution multi-purpose device for detecting cosmic-rays including electrons,γ-rays,protons and heavy ions in an energy range of a few GeV to 100 *** has been launched on December 17th,2015 and operates on a sun-synchronous orbit at the altitude of 500 *** consists of four sub-detectors:a Plastic Scintillator Detector(PSD),a Silicon-Tungsten Tracker(STK),a Bismuth Germanate Oxid Calorimeter(BGO)and a NeUtron Detector(NUD).

关键词： damp altitude Plastic

Intelligent identification of underground powerhouse of pumped-storage power plant

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Acta Mechanica Sinica 2005年第2期21卷 187-191页

作者： WanquanSun ZhenyueMa XudongYan JunxiuQi XiaojingDu BeijingHydroelectricDesign&InvestigationInstituteofNationalElectricCompany Beijing100024China DepartmentofCivilEngineering DalianUniversityotTechnologyDalian116024China

The damping coefficient and dynamical load characteristics are sensitive to the dynamic response of the underground powerhouse. In this paper, based on results of dynamic identification of the underground powerhouse of a pumped-storage power plant previously made, the immune algorithm is used together with the ANSYS Code and the high-frequency water pressure pulsation during a routine operation and the damping ratio are identified and studied with observed data. This will be useful for generating a precise numerical model and studying the hydropower station.

关键词： Immune algorithm Finite element method Identification High-frequency water pressure pulsation damp

The novel peptide Nb PPI1 identified from Nicotiana benthamiana triggers immune responses and enhances resistance against Phytophthora pathogens

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Journal of Integrative Plant Biology 2021年第5期63卷 961-976页

作者： Qujiang Wen Manli Sun Xianglan Kong Yang Yang Qiang Zhang Guiyan Huang Wenqin Lu Wanyue Li Yuling Meng Weixing Shan State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection Northwest A&F UniversityYangling 712100China State Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy Northwest A&F UniversityYangling 712100China State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences Northwest A&F UniversityYangling 712100China

In plants, recognition of small secreted peptides, such as damage/danger-associated molecular patterns(damps), regulates diverse processes,includingstressandimmune responses. Here, we identified an SGPS(Ser-Gly-Pro-Ser) motif-containing peptide, Nicotiana tabacum NtPROPPI, and its two homologs in Nicotiana benthamiana, NbPROPPI1 and NbPROPPI2. Phytophthora parasitica infection and salicylic acid(SA) treatment induced NbPROPPI1/2 expression. Moreover, SignalP predicted that the 89-amino acid NtPROPPI includes a 24-amino acid N-terminal signal peptide and NbPROPPI1/2-GFP fusion proteins were mainly localized to the periplasm. Transient expression of NbPROPPI1/2 inhibited ***,andNbPROPPI1/2 knockdown rendered plants more susceptible to P. parasitica. An eight-amino-acid segment in the NbPROPPI1 C-terminus was essential for its immune function and a synthetic 20-residue peptide, NbPPI1, derived from the C-terminus of NbPROPPI1 provoked significant immune responses in N. benthamiana. These responses led to enhanced accumulation of reactive oxygen species, activation of mitogen-activated protein kinases, and up-regulation of the defense genes Flg22-induced receptor-like kinase(FRK) and WRKY DNA-binding protein 33(WRKY33). The NbPPI1-induced defense responses require Brassinosteroid insensitive1-associated receptor kinase 1(BAK1). These results suggest that NbPPI1 functions as a damp in N. benthamiana;this novel damp provides a potentially useful target for improving plant resistance to Pytophthora pathogens.

关键词： damp disease resistance Phytophthora SGPS peptides plant immunity

The Capacitive Coupling Modalities for Oncological Hyperthermia

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Open Journal of Biophysics 2021年第3期11卷 252-313页

作者： Andras Szasz Department of Biotechnics St. Istvan University Buda&ouml rs Hungary

The local-regional oncological hyperthermia has various electromagnetic methods for energy-transfer. The differences involve conceptual considerations and technical solutions. The most frequently applied energy transfer is capacitive coupling, concentrating the electric field to be the active heating component. The realization of the capacitive coupling set-up is divided into two different categories based on their goals for heating: 1) the homogeneous (conventional) heating, using isothermal conditions for dosing, and 2) the selective heterogeneous heating, using cellularly absorbed energy for dosing. The homogeneous heating utilizes plane-wave matching, absorbing the wave for energy transfer. The heterogenic heating uses impedance matching, selecting the malignant cells by their electromagnetic specialties, like their heterogenic impedance, higher membrane-raft density, and different spatio-temporal (pathologic pattern) arrangements. This article’s objective is to compare and discuss the details of the two kinds of capacitive coupling techniques.

关键词： Plane-Wave Matching Impedance Matching Apoptosis damp ICD Selective Heating Electromagnetic Heterogeneity Membrane Raft