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Cross-linked Polyethylenimine as Potential DNA Vector for Gene Delivery with High Efficiency and Low cytotoxicity

Acta Biochimica et Biophysica Sinica 2006年第11期38卷 780-787页

作者： Wei DONG~(1,2) Guang-Hui JIN~1 Shu-Feng LI~(1,3) Qi-Ming SUN~1 Ding-Yuan MA~1 Zi-Chun HUA~1 1 State Key Laboratory of Pharmaceutical Biotechnology,College of Life Science,Nanjing University,Nanjing 210093,China 2 School of Chemical Engineering Nanjing University of Science and Technology,Nanjing 210094,China 3 College of Basic Medical Science,Southeast University,Nanjing 210009,China State Key Laboratory of Pharmaceutical Biotechnology College of Life Science Nanjing UniversityNanjing 210093 China School of Chemical Engineering Nanjing University of Science and TechnologyNanjing 210094 China College of Basic Medical Science Southeast UniversityNanjing 210009 China

Polyethylenimine (PEI) has been known as an efficient gene carrier with the highest cationiccharge *** transfection efficiency of PEI,along with its cytotoxicity,strongly depends on itsmolecular *** enhance its gene delivery efficiency and minimize cytotoxicity,we have synthesizedsmall cross-linked PEI with biodegradable linkages and evaluated their transfection efficiencies in *** study,branched PEI with a molecular weight of 800 Da was cross-linked by small diacrylate[1,4-butanediol diacrylate or ethyleneglycol dimethacrylate (EGDMA)] for 2-6 *** efficiencies of thecross-linked PEI in in vitro transfection of plasmid DNA containing enhanced green fluorescent protein(EGFP) reporter gene were assessed in melanoma B 16F10 cell line and other cell *** cytometrywas used to quantify the cellular entry efficiency of plasmid and the transgene expression *** of the cross-linked PEI in these cells were evaluated by MTT ***-PEI 800-4h,atypical cross-linked PEI reported here,mediated a more efficient expression of reporter gene than thecommercially available 25-kDa branched PEI control,and resulted in a 9-fold increase in gene deliveryin B16F10 cells and a 16-fold increase in 293T cells,while no cytotoxicity was found at the optimizedcondition for gene ***,the transfection activity of polyplexes was preserved in thepresence of serum proteins.

关键词： polyethylenimine gene delivery cytotoxicity B16F10 293T cell line

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Effect of Gd3+-,Pr3+-or Sm3+-substituted cobalt-zinc ferrite on photodegradation of methyl orange and cytotoxicity tests

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Journal of Rare Earths 2019年第12期37卷 1288-1295页

作者： Seyed Mahdi Peymani-Motlagh Nadia Moeinian Mojtaba Rostami Mahdi Fasihi-Ramandi Ali Sobhani-Nasab Mehdi Rahimi-Nasrabadi Mohammad Eghbali-Arani Mohammad Reza Ganjali Teofil Jesionowski Hermann Ehrlich Mohammad Ali Karimi Narges Ajami Department of Chemistry Imam Hossein UniversityTehran 16765-3454Iran Department of Chemistry Payame Noor UniversityTehran 19395-4697Iran Young Researchers and Elite Club Karaj BranchIslamic Azad UniversityKaraj ***Iran Molecular Biology Research Center Systems Biology and Poisoning InstituteBaqiyatallah University of Medical SciencesTehran ***Iran Social Determinants of Health(SDH)Research Center Kashan University of Medical SciencesKashan ***Iran Core Research Lab Kashan University of Medical SciencesKashan ***Iran Faculty of Pharmacy Baqiyatallah University of Medical SciencesTehran 195163759Iran Department of Physics University of KashanKashan ***Iran Center of Excellence in Electrochemistry Faculty of ChemistryUniversity of TehranTehran ***Iran Institute of Chemical Technology and Engineering Faculty of Chemical TechnologyPoznan University of TechnologyPoznan 60965Poland Institute of Electronic and Sensor Materials TU Bergakademie FreibergFreiberg 09599Germany

Gd3+-,Pr3+-or Sm3+-doped Co-Zn(Co0.5Zn0.5Fe2 O4)magnetic ferrites(i.e.,Co0.5Zn0.5Gd0.1Fe1.9O4,Co0.5Zn0.5Pr0.1Fe1.9O4 and Co0.5Zn0.5Sm0.1Fe1.9O4)were prepared using a facile sol-gel approach,and the structure,surface morphology and chemical composition of the products were studied by means of scanning electron microscopy(SEM),energy dispersive X-ray analysis(EDX),X-ray diffraction(XRD),UVvisible diffuse reflectance spectroscopy(DRS),photoluminescence(PL)spectroscopy,Fourier transform infrared spectroscopy(FT-IR)and vibrating sample magnetometer(VSM)*** patterns show the Co-Zn product is composed of cubic spinel phases with few impurities or secondary phases,and the average crystallite sizes of the samples are determined to be approximately^51—80,~99—181,~68—103 and^83—133 *** the coercivity and remnant and saturation magnetizations,evaluated by vibrating sample magnetometer(VSM),are found to increase linearly with the incorporation of Gd3+,Pr3+and Sm3+in the product *** CO1-xZnxFe2-yRyO4 photocatalyst sample is found to display a red shift in its absorption,and exhibits outstanding photocatalytic effects in the degradation of MO under ultraviolet(UV)*** is attributed to the reduction of the band gap of cobalt-zinc ferrite due to the presence of rare earth *** in vitro evaluations of the cytotoxic effects of the synthesized nanoparticles were performed on a HeLa cell line.

关键词： Magnetic ferrite Rare earth ions Photocatalyst cytotoxicity Sol-gel

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Removal of ammonia from aqueous solutions by catalytic oxidation with copper-based rare earth composite metal materials: catalytic performance, characterization, and cytotoxicity evaluation

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Journal of Rare Earths 2011年第7期29卷 632-637页

作者：洪彰懋 Department of Vehicle Engineering Yung-Ta Institute of Technology and Commerce 316 Chung-Shan Road Linlo Pingtung 909 Taiwan China

Ammonia （NH3） has an important use in the chemical industry and is widely found in industrial wastewater. For this investigation of copper-based rare earth composite metal materials, aqueous solutions containing 400 mg/L of ammonia were oxidized in a batch-bed reac-tor with a catalyst prepared by the co-precipitation of copper nitrate, lanthanum nitrate and cerium nitrate. Barely any of the dissolved ammo-nia was removed by wet oxidation without a catalyst, but about 88% of the ammonia was reduced during wet oxidation over the catalysts at 423 K with an oxygen partial pressure of 4.0 MPa. The catalytic redox behavior was determined by cyclic voltammetry （CV）. Furthermore, the catalysts were characterized using thermogravimetric analyzer （TGA） and scanning electron microscope-energy dispersive X-ray spectroscopy （SEM-EDX）, which showed that the catalytic behavior was related to the metal oxide properties of the catalyst. In addition, the copper-lanthanum-cerium composite-induced cytotoxicity in the human lung MRC-5 cell line was tested, and the percentage cell survival was determined by 3-（4,5-dimethylthiazol-2-yl）-5-（3-carboxymethoxyphenyl）-2-（4-sulfophenyl）-2H-tetra-zolium （MTS） analysis in vitro. No ap-parent cytotoxicity was observed when the human lung cells were exposed to the copper-lanthanum-cerium composite.

关键词： wet oxidation ammonia copper-lanthanum-cerium composite metal catalyst cytotoxicity rare earths

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Dynamic nano-Ag colloids cytotoxicity to and accumulation by Escherichia coli:Effects of Fe^3+,ionic strength and humic acid

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Journal of Environmental Sciences 2020年第3期32卷 180-193页

作者： Weicheng Zhang Bingyu Ning Caiyun Sun Ke Song Xin Xu Tao Fang Lunguang Yao Collaborative Innovation Center of Water Security for Water Source Region of Mid-line of South-to-North Diversion Project of Henan Province Nanyang Normal UniversityNanyang 473061China Henan Key Laboratory of Ecological Security for Water Source Region of Mid-line of South-to-North Diversion Project Nanyang Normal UniversityNanyang 473061China State Key Laboratory of Motor Vehicle Biofuel Technology Nanyang 473000China Institute of Hydrobiology Chinese Academy of SciencesWuhan 430072China University of Chinese Academy of Sciences Beijing 100049China

Released Ag ions or/and Ag particles are believed to contribute to the cytotoxicity of Ag nanomaterials,and thus,the cytotoxicity and mechanism of Ag nanomaterials should be dynamic in water due to unfixed Ag particle:Ag+ *** recent research found that the cytotoxicity of PVP-Ag nanoparticles is attributable to Ag particles alone in 3 hr bioassays,and shifts to both Ag particles and released Ag^+ in 48 hr ***,as a continued study,the cytotoxicity and accumulation of 50 and 100 nm Ag colloids in Escherichia coli were determined *** cytotoxicity and mechanisms of nanoAg colloids are dynamic throughout exposure and are derived from both Ag ions and *** accumulation by *** is derived mainly from extracellular Ag particles during the initial 12 hr of exposure,and thereafter mainly from intracellular Ag ***^3+ accelerates the oxidative dissolution of nano-Ag colloids,which results In decreasing amounts of Ag particles and particle-related ***^+ stabilizes nano-Ag colloids,thereby decreasing the bioavailability of Ag particles and particle-related *** acid(HA) binds Ag^+ to form Ag^+-HA,decreasing ion-related toxicity and binding to the *** surface,decreasing particle-related *** in complex conditions showed a stronger relative contribution to toxicity and accumulation than Na^+ or Fe^3+.The results highlighted the cytotoxicity and mechanism of nano-Ag colloids are dynamic and affected by environmental factors,and therefore exposure duration and water chemistry should be seriously considered in environmental and health risk assessments.

关键词： cytotoxicity mechanism Accumulation Nano-Ag colloids Escherichia coli Environmental factors

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Preparation and characterization of nano-rare earth composite materials:application in selectivity catalytic oxidation of ammonia and its cytotoxicity study

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Journal of Rare Earths 2010年第S1期28卷 362-366页

作者： Chang-Mao Hung Department of Vehicle Engineering Yung-Ta Institute of Technology and Commerce316 Chung-Shan RoadLinloPingtung 909Taiwan

The manufacture,physical characterization,environmental applications and cytotoxicity properties of nanocomposites consisting of CuO/CeO2 nano-rare earth composite materials prepared using the coprecipitation method at molar ratio of 6:4 with aqueous solutions of copper nitrate and cerium nitrate were *** performance of the selective catalytic oxidation of ammonia to N2(NH3-SCO) over a CuO/CeO2 nano-rare earth composite materials in a tubular fixed-bed reactor(TFBR) at temperatures from 423 to 673 K in the presence of oxygen was *** catalytic redox behavior was determined by cyclic voltammetry(CV).The nanocomposite particles were characterized by TEM,with a tiny particle size around 10 nm with high dispersion ***,cell cytotoxicity and the percentage cell survival were determined by using 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethoxyphenol)-2-(4-sulfophenyl)-2H-tetra-zolium(MTS) assay on human lung MRC-5 cell *** results showed that no apparent cytotoxicity was observed when the MRC-5 was exposed to the CuO/CeO2 nanocomposite materials.

关键词： selective catalytic oxidation (SCO) tubular fixed-bed reactor (TFBR) ammonia CuO/CeO2 nanocomposite material cytotoxicity rare earths

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Synthesis, Characterization and cytotoxicity of Ammine/Methylamine Platinum(Ⅱ) Complexes with Carboxylates

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Chemical Research in Chinese Universities 2006年第3期22卷 278-282页

作者： ZHANG Jin-chao SHEN Yong YANG Meng-su Department of Chemistry College of Chemistry ＆ Environmental Science Hebei University Baoding 071002 P. R. China Department of Biology and Chemistry City University of Hong Kong Hong Kong P. R. China

Seven novel platinum （Ⅱ） complexes [ Pt（Ⅱ）（NH3）（CH3NH2）X2] （Ⅰ-Ⅶ ）（ X： CH3COO^-, CH2CICOO^- , CHCI2COO^- , C6H5-COO^- , p-CH3O--C6H4-COO^- , p-NH2-C6H4-COO^- , p-NO2--C6H4- COO^-） were prepared and characterized by means of elemental analysis, molar conductivity, thermal analysis, IR, UV, and ^1H NMR spectrometries. The cytotoxicity against HCT-8, BGC-823, MCF-7, EJ, and HL-60 cell lines increases in the following sequence： cisplatin 〉 Ⅳ 〉 Ⅴ 〉 Ⅵ 〉 Ⅶ 〉 Ⅰ 〉 Ⅱ 〉 Ⅲ. Moreover, the complexes （ Ⅰ --Ⅻ） display substantially greater activities agaist EJ and HL-60 cell lines than those against the cell lines from other carcinomas. They can induce a concentration-dependent accumulation of HL-60 cells in the G2/M phase of the cell cycle as cisplatin. There is no significant correlation between total DNA platination levels and cytotoxicity of the complexes.

关键词： Ammine/methylamine platinum(Ⅱ) complex Synthesis cytotoxicity Cell cycle: DNA-binding

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cytotoxicity and genome-wide microarray analysis of intestinal smooth muscle cells in response to hexavalent chromium induction

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Zoological Research 2013年第3期34卷 93-100页

作者： Li-Fang JIN Yuan-Yuan WANG Zi-Dong ZHANG Yi-Meng YUAN Yi-Rui HU Yang-Feng WEI Jian NI College of Life Science of Shaoxing University Shaoxing Zhejiang 312000China

Chronic ingestion of high concentrations of hexavalent chromium[Cr(VI)]in drinking water induces intestinal tumors in mice;however,information on its toxicity on intestinal smooth muscle cells is *** present study aimed to assess the in vitro and in vivo toxicological effects of Cr(VI)on intestinal smooth muscle *** intestinal smooth muscle cells(HISM cells)were cultured with different concentrations of Cr(VI)to evaluate effects on cell proliferation ability,oxidative stress levels,and antioxidant ***,tissue sections in Cr(VI)exposed rabbits were analyzed to evaluate toxicity on intestinal muscle cells in *** chips were utilized to assess differential gene expression profiles at the genome-wide level in 1μmol/L Cr(VI)treated *** tissue biopsy results showed that Cr(VI)increased the incidences of diffuse epithelial hyperplasia in intestinal jejunum but caused no obvious damage to the structure of the *** proliferation analysis revealed that high concentrations(≥64μmol/L)but not low concentrations of Cr(VI)(≤16μmol/L)significantly inhibited the growth of HISM *** oxidative stress levels,the expression of reactive oxygen species(ROS)and nitric oxide(NO)was elevated at high concentrations(≥64μmol/L)but not at low concentrations of Cr(VI)(≤16μmol/L).In addition,dose-dependent increases in the activity of oxidized glutathione(GSSH)/total-glutathione(T-GSH)were also *** chip screened 491 differentially expressed genes including genes associated with cell apoptosis,oxidations,and *** of these differentially expressed genes may be unique to smooth muscle cells in response to Cr(VI)induction.

关键词： Intestinal smooth muscle cells Hexavalent chromium cytotoxicity Gene chip

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Expression of human FUS/TLS in yeast leads to protein aggregation and cytotoxicity,recapitulating key features of FUS proteinopathy

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Protein & Cell 2011年第2期2卷 141-149页

作者： Kazuo Fushimi Charles Long Neha Jayaram Xiaoping Chen Liming Li Jane Y.Wu Department of Neurology Center for Genetic MedicineLurie Comprehensive Cancer CenterNorthwestern University Feinberg School of Medicine303 E.SuperiorChicagoIL 60611USA Department of Molecular Pharmacology and Biological Chemistry Northwestern University Feinberg School of Medicine303 E.SuperiorChicagoIL 60611USA

Mutations in the fused in sarcoma/translocated in liposarcoma(FUS/TLS)gene have been associated with amyotrophic lateral sclerosis(ALS).FUS-positive neuropathology is reported in a range of neurodegenerative diseases,including ALS and fronto-temporal lobar degeneration with ubiquitin-positive pathology(FTLDU).To examine protein aggregation and cytotoxicity,we expressed human FUS protein in *** of either wild type or ALS-associated R524S or P525L mutant FUS in yeast cells led to formation of aggregates and cytotoxicity,with the two ALS mutants showing increased ***,yeast cells expressing human FUS protein recapitulate key features of FUSpositive neurodegenerative ***,a significant fraction of FUS expressing yeast cells stained by propidium iodide were without detectable protein aggregates,suggesting that membrane impairment and cellular damage caused by FUS expression may occur before protein aggregates become microscopically detectable and that aggregate formation might protect cells from FUS-mediated *** N-terminus of FUS,containing the QGSY and G rich regions,is sufficient for the formation of aggregates but not *** C-terminal domain,which contains a cluster of mutations,did not show aggregation or *** to TDP-43 when expressed in yeast,FUS protein has the intrinsic property of forming aggregates in the absence of other human *** the other hand,the aggregates formed by FUS are thioflavin T-positive and resistant to 0.5%sarkosyl,unlike TDP-43 when expressed in yeast ***,TDP-43 and FUS display distinct domain requirements in aggregate formation and cytotoxicity.

关键词： FUS/TLS protein aggregation cytotoxicity

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Type 1 CD8^+ T Cells are Superior to Type 2 CD8^+ T Cells in Tumor Immunotherapy due to Their Efficient cytotoxicity,Prolonged Survival and Type 1 Immune Modulation

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Cellular & Molecular Immunology 2007年第4期4卷 277-285页

作者： Zhenmin Ye Chaoke Tang Shulin Xu Bei Zhang Xueshu Zhang Terence Moyana Jicheng Yang Jim Xiang Research Unit Saskatchewan Cancer Agency Departments of Oncology and Immunology College of Medicine University of Saskatchewan Saskatoon Saskatchewan S7N4H4 Canada Department of Pathology and Laboratory Medicine University of Ottawa Ottawa Ontario K1H 8L6 Canada Cell and Molecular Biology Institute College of Medicine Soochow University Suzhou 215132 China Ye and Tang made the same contribution to the study.

CD8^＋ cytotoxic T （Tc） cells play a crucial role in host immune responses to cancer, and in this context, adoptive CD8^＋ Tc cell therapy has been studied in numerous animal tumor models. Its antitumor efficacy is, to a large extent, determined by the ability of Tc cells to survive and infiltrate tumors. In clinical trials, such in vitro-activated T cells often die within hours to days, and this greatly limits their therapeutic efficacy. CD8^＋ Tc cells fall into two subpopulations based upon their differential cytokine secretion. In this study, we in vitro generated that ovalbumin （OVA）-pulsed dendritic cell （DCovA）-activated CD8^＋ type 1 Tc （Tcl） cells secreting IFN-T, and CD8^＋ type 2 Tc （Tc2） cells secreting IL-4, IL-5 and IL-10, which were derived from OVA-specific T cell receptor （TCR） transgenic OT I mice. We then systemically investigated the in vitro and in vivo effector function and survival of Tcl and Tc2 cells, and then assessed their survival kinetics after adoptively transferred into C57BL/6 mice, respectively. We demonstrated that, when compared to CD8^＋ Tc2, Tcl cells were significantly more effective in perforin-mediated cytotoxicity to tumor cells, had a significantly higher capacity for in vivo survival after the adoptive T cell transfer, and had a significantly stronger therapeutic effect on eradication of well-established tumors expressing OVA in animal models. In addition, CD8^＋ Tcl and Tc2 cells skewed the phenotype of CD4^＋ T cells toward Thl and Th2 type, respectively. Therefore, the information regarding the differential effector function, survival and immune modulation of CD8^＋ Tcl and Tc2 cells may provide useful information when preparing in vitro DC-activated CD8^＋ T cells for adoptive T cell therapy of cancer.

关键词： Tc1 cell Tc2 cell cytokine profile cytotoxicity survival tumor therapy

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Serum-free culture of H pylori intensifies cytotoxicity

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World Journal of Gastroenterology 2007年第4期13卷 532-537页

作者： Hiroyuki Ohno Akiyuki Murano Department of Medicine and Clinical Oncology Graduate School of Medicine Chiba University Chiba 260-8670 Japan Department of Clinical Molecular Biology Graduate School of Medicine Chiba University Chiba 260-8670 Japan

AIM： To perform a long culture passage of H pylori without serum, taking into account its cytotoxicity and the presence of the probable new cytotoxic factor. METHODS： One sample of H pylon 60190 （ATCC 49503） was grown on Brain Heart Infusion （BHI） agar containing 0.5% 2,6-di-O-methyl-β-cyclodextrin without any serum, being passaged 70-100 times every 3-4 d for approximately 2 h, while another sample of H pylori contained 70 mL/L fetal calf serum without 2,6-di-O- methyl-β-cyclodextrin. Their supernatant and extract after 16 h in culture were evaluated for changes in cell morphology and for cell viability using HeLa cells. Furthermore, the characteristics of the probable cytotoxic factor in the extract were examined on partial purification studies and its oytotoxicity was evaluated in various human cells. RESULTS： The supernatant and the extract of the bacterium grown on serum-free medium had strong cytotoxicity compared with those grown on serumcontaining medium. They irreversibly damaged HeLa cells without vacuolation that was altogether different from that of the bacterium when grown with serum. Their cytotoxicity was easily measured by cell viability assay. The probable cytotoxic factor partially purified and detected by chromatography had characteristics difference from that of vacuolating toxin and a broad cytotoxicity toward various cell lines. CONCLUSION： Serum-free long culture method of H pylorl makes its supernatant and its extract cytotoxic enough to be easily measured by cell viability assay. The probable cytotoxic factor has a unique characteristic and might be a new cytotoxin.

关键词： H pylori cytotoxicity Serum-free longculture

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